Gene Symbol: PAN1
Description: Pan1p
Alias: DIM2, MDP3, MIP3, Pan1p
Species: Saccharomyces cerevisiae S288c
Products:     PAN1

Top Publications

  1. Aguilar R, Watson H, Wendland B. The yeast Epsin Ent1 is recruited to membranes through multiple independent interactions. J Biol Chem. 2003;278:10737-43 pubmed phosphoinositides in vitro and that Ent1 NPF motifs interact with the EH domain-containing proteins Ede1 and Pan1. Our findings indicate that the ENTH domain interaction with membrane lipids cooperates with the binding of ..
  2. Sun Y, Martin A, Drubin D. Endocytic internalization in budding yeast requires coordinated actin nucleation and myosin motor activity. Dev Cell. 2006;11:33-46 pubmed
    ..These results reveal a mechanism in which actin nucleation and myosin motor activity cooperate to promote endocytic internalization. ..
  3. Tang H, Munn A, Cai M. EH domain proteins Pan1p and End3p are components of a complex that plays a dual role in organization of the cortical actin cytoskeleton and endocytosis in Saccharomyces cerevisiae. Mol Cell Biol. 1997;17:4294-304 pubmed
    ..Included in this new protein family, termed EH domain proteins, are two yeast proteins, Pan1p and End3p...
  4. Duncan M, Cope M, Goode B, Wendland B, Drubin D. Yeast Eps15-like endocytic protein, Pan1p, activates the Arp2/3 complex. Nat Cell Biol. 2001;3:687-90 pubmed
    ..Here we show that the yeast endocytic protein Pan1p binds to and activates the Arp2/3 complex...
  5. Galletta B, Chuang D, Cooper J. Distinct roles for Arp2/3 regulators in actin assembly and endocytosis. PLoS Biol. 2008;6:e1 pubmed publisher
    ..Knowledge of these roles provides insight into the in vivo relevance of the dendritic nucleation model for actin assembly. ..
  6. Wendland B, Emr S. Pan1p, yeast eps15, functions as a multivalent adaptor that coordinates protein-protein interactions essential for endocytosis. J Cell Biol. 1998;141:71-84 pubmed
    ..b>Pan1p is a homologue of the mammalian protein eps15, which has been implicated in endocytosis by virtue of its ..
  7. Gagny B, Wiederkehr A, Dumoulin P, Winsor B, Riezman H, Haguenauer Tsapis R. A novel EH domain protein of Saccharomyces cerevisiae, Ede1p, involved in endocytosis. J Cell Sci. 2000;113 ( Pt 18):3309-19 pubmed
    ..Two of the yeast proteins, End3p and Pan1p, are required for the internalization step of endocytosis...
  8. Kaksonen M, Sun Y, Drubin D. A pathway for association of receptors, adaptors, and actin during endocytic internalization. Cell. 2003;115:475-87 pubmed
    ..Attention is now focused on the mechanisms by which the order and timing of events in this endocytic pathway are achieved. ..
  9. Toshima J, Toshima J, Duncan M, Cope M, Sun Y, Martin A, et al. Negative regulation of yeast Eps15-like Arp2/3 complex activator, Pan1p, by the Hip1R-related protein, Sla2p, during endocytosis. Mol Biol Cell. 2007;18:658-68 pubmed
    ..The yeast Eps15-related Arp2/3 complex activator, Pan1p, is essential for endocytic internalization and proper actin organization...

More Information


  1. Toshima J, Toshima J, Martin A, Drubin D. Phosphoregulation of Arp2/3-dependent actin assembly during receptor-mediated endocytosis. Nat Cell Biol. 2005;7:246-54 pubmed
    ..The Eps15-like yeast protein Pan1p is a Prk1p substrate that is essential for endocytic internalization and for proper actin organization...
  2. Tang H, Xu J, Cai M. Pan1p, End3p, and S1a1p, three yeast proteins required for normal cortical actin cytoskeleton organization, associate with each other and play essential roles in cell wall morphogenesis. Mol Cell Biol. 2000;20:12-25 pubmed
    The EH domain proteins Pan1p and End3p of budding yeast have been known to form a complex in vivo and play important roles in organization of the actin cytoskeleton and endocytosis...
  3. Zoladek T, Tobiasz A, Vaduva G, Boguta M, Martin N, Hopper A. MDP1, a Saccharomyces cerevisiae gene involved in mitochondrial/cytoplasmic protein distribution, is identical to the ubiquitin-protein ligase gene RSP5. Genetics. 1997;145:595-603 pubmed
    ..MDP3 is identical to PAN1, which encodes a protein involved in initiation of translation and actin cytoskeleton organization...
  4. Huang B, Cai M. Pan1p: an actin director of endocytosis in yeast. Int J Biochem Cell Biol. 2007;39:1760-4 pubmed
    The yeast protein Pan1p plays a key role in actin-driven endocytosis. The molecular architecture enables the protein to perform multivalent tasks...
  5. Howard J, Hutton J, Olson J, Payne G. Sla1p serves as the targeting signal recognition factor for NPFX(1,2)D-mediated endocytosis. J Cell Biol. 2002;157:315-26 pubmed
    ..Thus, through a novel NPF-binding domain, Sla1p serves as an endocytic targeting signal adaptor, providing a means to couple cargo with clathrin- and actin-based endocytic machineries. ..
  6. Wendland B, McCaffery J, Xiao Q, Emr S. A novel fluorescence-activated cell sorter-based screen for yeast endocytosis mutants identifies a yeast homologue of mammalian eps15. J Cell Biol. 1996;135:1485-500 pubmed
    ..Complementation cloning of the DIM genes revealed identity of DIM1 to SHE4 and DIM2 to PAN1. Pan1p shares homology with the mammalian clathrin adaptor-associated protein, eps15...
  7. D Agostino J, Goode B. Dissection of Arp2/3 complex actin nucleation mechanism and distinct roles for its nucleation-promoting factors in Saccharomyces cerevisiae. Genetics. 2005;171:35-47 pubmed
    ..We examined genetic relationships among the four S. cerevisiae NPFs. Combining las17delta with pan1-101 or myo3delta myo5delta was lethal at all temperatures, whereas combining pan1-101 with myo3delta myo5delta ..
  8. Zeng G, Cai M. Regulation of the actin cytoskeleton organization in yeast by a novel serine/threonine kinase Prk1p. J Cell Biol. 1999;144:71-82 pubmed
    Normal actin cytoskeleton organization in budding yeast requires the function of the Pan1p/ End3p complex. Mutations in PAN1 and END3 cause defects in the organization of actin cytoskeleton and endocytosis...
  9. Tang H, Cai M. The EH-domain-containing protein Pan1 is required for normal organization of the actin cytoskeleton in Saccharomyces cerevisiae. Mol Cell Biol. 1996;16:4897-914 pubmed
    ..isolating rapid-death mutants in the background of the Start-deficient cdc28-4 mutation, the essential yeast gene PAN1, previously thought to encode the yeast poly(A) nuclease, is identified as a new factor required for normal ..
  10. Whitworth K, Bradford M, Camara N, Wendland B. Targeted disruption of an EH-domain protein endocytic complex, Pan1-End3. Traffic. 2014;15:43-59 pubmed publisher
    b>Pan1 is a multi-domain scaffold that enables dynamic interactions with both structural and regulatory components of the endocytic pathway...
  11. Monastyrska I, He C, Geng J, Hoppe A, Li Z, Klionsky D. Arp2 links autophagic machinery with the actin cytoskeleton. Mol Biol Cell. 2008;19:1962-75 pubmed publisher
    ..We propose that proteins of the Arp2/3 complex regulate Atg9 transport for specific types of autophagy. ..
  12. Feliciano D, Di Pietro S. SLAC, a complex between Sla1 and Las17, regulates actin polymerization during clathrin-mediated endocytosis. Mol Biol Cell. 2012;23:4256-72 pubmed publisher
    ..These results advance our understanding of the regulation of actin polymerization in endocytosis. ..
  13. Reiter W, Anrather D, Dohnal I, Pichler P, Veis J, Grøtli M, et al. Validation of regulated protein phosphorylation events in yeast by quantitative mass spectrometry analysis of purified proteins. Proteomics. 2012;12:3030-43 pubmed publisher
    ..We exemplify the value of such a strategy by an in-depth analysis of Pan1, a highly phosphorylated factor involved in early steps of endocytosis...
  14. Chi R, Torres O, SEGARRA V, Lansley T, Chang J, Newpher T, et al. Role of Scd5, a protein phosphatase-1 targeting protein, in phosphoregulation of Sla1 during endocytosis. J Cell Sci. 2012;125:4728-39 pubmed publisher
    ..This was dependent on the LxxQxTG repeats (SR) of Sla1, which are phosphorylated by Prk1 and bind Pan1, another Prk1 target, in the dephosphorylated state...
  15. Watson H, Cope M, Groen A, Drubin D, Wendland B. In vivo role for actin-regulating kinases in endocytosis and yeast epsin phosphorylation. Mol Biol Cell. 2001;12:3668-79 pubmed
    ..Interactions between the endocytic proteins Pan1p and End3p may be regulated by Prk1p-dependent threonine phosphorylation of Pan1p within the consensus sequence [L/..
  16. Suzuki R, Toshima J, Toshima J. Regulation of clathrin coat assembly by Eps15 homology domain-mediated interactions during endocytosis. Mol Biol Cell. 2012;23:687-700 pubmed publisher
    ..Several lines of evidence suggest that the yeast EH domain-containing proteins Pan1p, End3p, and Ede1p play important roles during endocytosis...
  17. Sun Y, Leong N, Jiang T, Tangara A, Darzacq X, Drubin D. Switch-like Arp2/3 activation upon WASP and WIP recruitment to an apparent threshold level by multivalent linker proteins in vivo. elife. 2017;6: pubmed publisher
    ..These studies provide evidence that WASP and WIP play central roles in establishment of a robust multivalent SH3 domain-PRM network in vivo, giving actin assembly onset at endocytic sites a switch-like behavior. ..
  18. Bidlingmaier S, Snyder M. Large-scale identification of genes important for apical growth in Saccharomyces cerevisiae by directed allele replacement technology (DART) screening. Funct Integr Genomics. 2002;1:345-56 pubmed
    ..We identified 29 insertion alleles, containing mutations in 17 different genes ( SMY1, SPA2, PAN1, SLA1, SLA2, CBK1, SEC22, FAB1, VPS36, VID22, RAS2, ECM33, OPI3, API1/YDR372c, API2/YDR525w, API3/YKR020w, and API4/..
  19. Pierce B, Toptygin D, Wendland B. Pan1 is an intrinsically disordered protein with homotypic interactions. Proteins. 2013;81:1944-63 pubmed publisher
    The yeast scaffold protein Pan1 contains two EH domains at its N-terminus, a predicted coiled-coil central region, and a C-terminal proline-rich domain...
  20. Hongay C, Jia N, Bard M, Winston F. Mot3 is a transcriptional repressor of ergosterol biosynthetic genes and is required for normal vacuolar function in Saccharomyces cerevisiae. EMBO J. 2002;21:4114-24 pubmed
    ..To learn more about Mot3 function, we have performed a synthetic lethal screen. This screen identified PAN1, a gene required for normal endocytosis, and VPS41, a gene required for vacuolar fusion and protein targeting, ..
  21. Sun Y, Leong N, Wong T, Drubin D. A Pan1/End3/Sla1 complex links Arp2/3-mediated actin assembly to sites of clathrin-mediated endocytosis. Mol Biol Cell. 2015;26:3841-56 pubmed publisher
    ..The yeast Eps15-related proteins Pan1 and End3 and the CIN85-related protein Sla1 are known to interact with each other in vitro, and they all appear ..
  22. Henry K, D hondt K, Chang J, Newpher T, Huang K, Hudson R, et al. Scd5p and clathrin function are important for cortical actin organization, endocytosis, and localization of sla2p in yeast. Mol Biol Cell. 2002;13:2607-25 pubmed
    ..Therefore, Scd5p and clathrin are important for actin organization and endocytosis, and Sla2p may provide a critical link between clathrin and the actin cytoskeleton in yeast, similar to HIP1(R) in animal cells. ..
  23. Miliaras N, Park J, Wendland B. The function of the endocytic scaffold protein Pan1p depends on multiple domains. Traffic. 2004;5:963-78 pubmed
    b>Pan1p is an essential protein of the yeast Saccharomyces cerevisiae that is required for the internalization step of endocytosis and organization of the actin cytoskeleton...
  24. Kaminska J, Sedek M, Wysocka Kapcinska M, Zoładek T. Characterization of nuclear localization and nuclear export signals of yeast actin-binding protein Pan1. FEBS Lett. 2007;581:5371-6 pubmed
    b>Pan1 is an actin patch-associated protein involved in endocytosis. Our studies revealed that in oleate-grown cells Pan1 is located in the nucleus as well as in patches...
  25. Liu K, Hua Z, Nepute J, Graham T. Yeast P4-ATPases Drs2p and Dnf1p are essential cargos of the NPFXD/Sla1p endocytic pathway. Mol Biol Cell. 2007;18:487-500 pubmed NPFXD endocytosis signal and its recognition by Sla1p, part of an endocytic coat/adaptor complex with clathrin, Pan1p, Sla2p/End4p, and End3p...
  26. Shih J, Reck Peterson S, Newitt R, Mooseker M, Aebersold R, Herskowitz I. Cell polarity protein Spa2P associates with proteins involved in actin function in Saccharomyces cerevisiae. Mol Biol Cell. 2005;16:4595-608 pubmed
    ..We identified Myo1p, Myo2p, Pan1p, and the protein encoded by YFR016c as proteins that interact with Spa2p...
  27. Zeng G, Yu X, Cai M. Regulation of yeast actin cytoskeleton-regulatory complex Pan1p/Sla1p/End3p by serine/threonine kinase Prk1p. Mol Biol Cell. 2001;12:3759-72 pubmed
    ..One possible function of Prk1p is the negative regulation of Pan1p, an actin patch regulatory protein that forms a complex in vivo with at least two other proteins, Sla1p and End3p...
  28. Tronnersjö S, Hanefalk C, Balciunas D, Hu G, Nordberg N, Murén E, et al. The jmjN and jmjC domains of the yeast zinc finger protein Gis1 interact with 19 proteins involved in transcription, sumoylation and DNA repair. Mol Genet Genomics. 2007;277:57-70 pubmed
    ..The human Sgs1 homolog WRN also interacts with the Gis1 jumonji domain. Finally, we note that several jumonji domain interactors are related to proteins that are found in mammalian PML nuclear bodies. ..
  29. Barker S, Lee L, Pierce B, Maldonado Baez L, Drubin D, Wendland B. Interaction of the endocytic scaffold protein Pan1 with the type I myosins contributes to the late stages of endocytosis. Mol Biol Cell. 2007;18:2893-903 pubmed
    The yeast endocytic scaffold Pan1 contains an uncharacterized proline-rich domain (PRD) at its carboxy (C)-terminus...
  30. Ziółkowska N, Karotki L, Rehman M, Huiskonen J, Walther T. Eisosome-driven plasma membrane organization is mediated by BAR domains. Nat Struct Mol Biol. 2011;18:854-6 pubmed publisher
    ..Our data reveal a previously unrecognized family of BAR-domain proteins involved in plasma membrane organization. ..
  31. Yaguchi S, Shen H, Tsurugi K. Localization of Gts1p in cortical actin patches of yeast and its possible role in endocytosis. Eur J Cell Biol. 2007;86:275-85 pubmed
    ..Whereas Gts1p does not directly bind to actin, it associated mainly with the actin-associated protein Pan1p. In the GTS1-deleted transformant gts1Delta, the number of cells containing either a fragmented vacuole or an ..
  32. Huang B, Chua L, Bose N, Cai M. Negative regulation of the actin-regulating kinase Prk1p by patch localization-induced autophosphorylation. Traffic. 2009;10:35-41 pubmed publisher
    ..In budding yeast, Prk1p phosphorylates numerous endocytic proteins including Pan1p and Sla1p. Prk1p has been observed to undergo autophosphorylation in vivo...
  33. Schafer D. Coupling actin dynamics and membrane dynamics during endocytosis. Curr Opin Cell Biol. 2002;14:76-81 pubmed
    ..Three of these proteins, Abp1p, Pan1p and cortactin, are activators of actin assembly nucleated by the Arp2/3 complex, a key regulator of actin assembly ..
  34. Chen C, Ingram M, Rosal P, Graham T. Role for Drs2p, a P-type ATPase and potential aminophospholipid translocase, in yeast late Golgi function. J Cell Biol. 1999;147:1223-36 pubmed
    ..These observations indicate a novel role for a P-type ATPase in late Golgi function and suggest a possible link between membrane asymmetry and clathrin function at the Golgi complex. ..
  35. Zeng G, Huang B, Neo S, Wang J, Cai M. Scd5p mediates phosphoregulation of actin and endocytosis by the type 1 phosphatase Glc7p in yeast. Mol Biol Cell. 2007;18:4885-98 pubmed
    b>Pan1p plays essential roles in both actin and endocytosis in yeast. It interacts with, and regulates the function of, multiple endocytic proteins and actin assembly machinery...
  36. Smaczynska de Rooij I, Costa R, Ayscough K. Yeast Arf3p modulates plasma membrane PtdIns(4,5)P2 levels to facilitate endocytosis. Traffic. 2008;9:559-73 pubmed publisher
    ..Our data provide evidence for a mechanism in yeast to positively regulate plasma membrane production of PtdIns(4,5)P2 levels and that these changes impact on endocytosis. ..
  37. Kaminska J, Wysocka Kapcinska M, Smaczyńska de Rooij I, Rytka J, Zoładek T. Pan1p, an actin cytoskeleton-associated protein, is required for growth of yeast on oleate medium. Exp Cell Res. 2005;310:482-92 pubmed
    b>Pan1p is a yeast actin cytoskeleton-associated protein localized in actin patches. It activates the Arp2/3 complex, which is necessary for actin polymerization and endocytosis...
  38. Jin M, Cai M. A novel function of Arp2p in mediating Prk1p-specific regulation of actin and endocytosis in yeast. Mol Biol Cell. 2008;19:297-307 pubmed
    The yeast protein Pan1p plays essential roles in actin cytoskeleton organization and endocytosis...
  39. Toshima J, Furuya E, Nagano M, Kanno C, Sakamoto Y, Ebihara M, et al. Yeast Eps15-like endocytic protein Pan1p regulates the interaction between endocytic vesicles, endosomes and the actin cytoskeleton. elife. 2016;5: pubmed publisher
    ..The Eps15-like protein Pan1p plays a key role in actin-mediated endocytosis and is negatively regulated by Ark1 and Prk1 kinases...
  40. Bradford M, Whitworth K, Wendland B. Pan1 regulates transitions between stages of clathrin-mediated endocytosis. Mol Biol Cell. 2015;26:1371-85 pubmed publisher
    ..Saccharomyces cerevisiae is a powerful model system with which to study clathrin-mediated endocytosis (CME). Pan1 is believed to be a scaffolding protein due to its interactions with numerous proteins that act throughout the ..