PAM17

Summary

Gene Symbol: PAM17
Description: Pam17p
Alias: FMP18, Pam17p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. van der Laan M, Chacinska A, Lind M, Perschil I, Sickmann A, Meyer H, et al. Pam17 is required for architecture and translocation activity of the mitochondrial protein import motor. Mol Cell Biol. 2005;25:7449-58 pubmed
    ..We have identified a sixth motor subunit. Pam17 (encoded by the open reading frame YKR065c) is anchored in the inner membrane and exposed to the matrix...
  2. Hutu D, Guiard B, Chacinska A, Becker D, Pfanner N, Rehling P, et al. Mitochondrial protein import motor: differential role of Tim44 in the recruitment of Pam17 and J-complex to the presequence translocase. Mol Biol Cell. 2008;19:2642-9 pubmed publisher
    ..by four membrane-associated cochaperones: the adaptor protein Tim44, the stimulatory J-complex Pam18/Pam16, and Pam17. It has been proposed that Tim44 serves as molecular platform to localize mtHsp70 and the J-complex at the TIM23 ..
  3. Chacinska A, van der Laan M, Mehnert C, Guiard B, Mick D, Hutu D, et al. Distinct forms of mitochondrial TOM-TIM supercomplexes define signal-dependent states of preprotein sorting. Mol Cell Biol. 2010;30:307-18 pubmed publisher
    ..Depending on the signals of the preproteins, switches between the different forms of supercomplex and TIM23 are required for the completion of preprotein import. ..
  4. Popov Celeketic D, Mapa K, Neupert W, Mokranjac D. Active remodelling of the TIM23 complex during translocation of preproteins into mitochondria. EMBO J. 2008;27:1469-80 pubmed publisher
    ..Two non-essential subunits of the complex, Tim21 and Pam17, modulate its activity in an antagonistic manner...
  5. Ting S, Yan N, Schilke B, Craig E. Dual interaction of scaffold protein Tim44 of mitochondrial import motor with channel-forming translocase subunit Tim23. elife. 2017;6: pubmed publisher
    ..Our data suggest a model in which the CTD serves primarily to anchor Tim44 to the translocon, whereas the NTD is a dynamic arm, interacting with multiple components to drive efficient translocation. ..
  6. Tamura Y, Endo T, Iijima M, Sesaki H. Ups1p and Ups2p antagonistically regulate cardiolipin metabolism in mitochondria. J Cell Biol. 2009;185:1029-45 pubmed publisher
    ..Furthermore, we observed synthetic growth defects in ups mutants in combination with loss of Pam17p, which controls the integrity of PAM...
  7. Schiller D. Pam17 and Tim44 act sequentially in protein import into the mitochondrial matrix. Int J Biochem Cell Biol. 2009;41:2343-9 pubmed publisher
    ..The recently identified non-essential Pam17 is a further member of PAM. Genetic and biochemical analyses reveal synthetic interactions between PAM17 and TIM44...
  8. Schilke B, Hayashi M, Craig E. Genetic analysis of complex interactions among components of the mitochondrial import motor and translocon in Saccharomyces cerevisiae. Genetics. 2012;190:1341-53 pubmed publisher
    ..We also report that higher amounts of Pam17, a nonessential motor component, are found associated with the translocon in both PAM16 and TIM44 mutants that ..
  9. Pareek G, Krishnamoorthy V, D Silva P. Molecular insights revealing interaction of Tim23 and channel subunits of presequence translocase. Mol Cell Biol. 2013;33:4641-59 pubmed publisher
    ..Our model conceptually explains the cooperative function between Tam41 and Pam17 subunits, while the antagonistic activity of Tim21 predominantly determines the bound and free forms of the PAM ..

More Information

Publications14

  1. Schendzielorz A, Schulz C, Lytovchenko O, Clancy A, Guiard B, Ieva R, et al. Two distinct membrane potential-dependent steps drive mitochondrial matrix protein translocation. J Cell Biol. 2017;216:83-92 pubmed publisher
    ..The presequence translocase constituent Pam17 is specifically recruited by the receptor Tim50 to promote the transport of hypersensitive precursors into the ..
  2. Lytovchenko O, Melin J, Schulz C, Kilisch M, Hutu D, Rehling P. Signal recognition initiates reorganization of the presequence translocase during protein import. EMBO J. 2013;32:886-98 pubmed publisher
    ..The signal-driven release of Tim21 from Tim50 promotes recruitment of Pam17 and thus triggers formation of the motor-associated form of the TIM23 complex required for matrix transport.
  3. Ting S, Schilke B, Hayashi M, Craig E. Architecture of the TIM23 inner mitochondrial translocon and interactions with the matrix import motor. J Biol Chem. 2014;289:28689-96 pubmed publisher
    ..cross-linker in the matrix exposed loop between transmembrane segments 1 and 2 (loop 1) cross-linked to Pam17. Alterations in this loop caused destabilization of the interaction of Pam17 with the translocon...
  4. Gebert M, Schrempp S, Mehnert C, Heißwolf A, Oeljeklaus S, Ieva R, et al. Mgr2 promotes coupling of the mitochondrial presequence translocase to partner complexes. J Cell Biol. 2012;197:595-604 pubmed publisher
    ..We conclude that Mgr2 is a coupling factor of the presequence translocase crucial for cell growth at elevated temperature and for efficient protein import. ..
  5. Schulz C, Rehling P. Remodelling of the active presequence translocase drives motor-dependent mitochondrial protein translocation. Nat Commun. 2014;5:4349 pubmed publisher
    ..Thus, a replenishment cycle of co-chaperones at the TIM23 complex is an integral part of Hsp70's ATPase cycle at the channel exit site and essential to maintain motor-driven mitochondrial protein import. ..