PAF1

Summary

Gene Symbol: PAF1
Description: Paf1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. ncbi A novel collection of accessory factors associated with yeast RNA polymerase II
    P A Wade
    Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver, Colorado, 80262, USA
    Protein Expr Purif 8:85-90. 1996
  2. ncbi Kinase Cak1 functionally interacts with the PAF1 complex and phosphatase Ssu72 via kinases Ctk1 and Bur1
    Carine Ganem
    Institut Curie, UMR2027 CNRS, Centre Universitaire, Orsay, France
    Mol Genet Genomics 275:136-47. 2006
  3. ncbi The stress-activated Hog1 kinase is a selective transcriptional elongation factor for genes responding to osmotic stress
    Markus Proft
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 23:241-50. 2006
  4. ncbi The Paf1 complex is essential for histone monoubiquitination by the Rad6-Bre1 complex, which signals for histone methylation by COMPASS and Dot1p
    Adam Wood
    Department of Biochemistry, Saint Louis University School of Medicine, St Louis, Missouri 63104, USA
    J Biol Chem 278:34739-42. 2003
  5. pmc Identification of Rkr1, a nuclear RING domain protein with functional connections to chromatin modification in Saccharomyces cerevisiae
    Mary A Braun
    Department of Biological Sciences, University of Pittsburgh, 269 Crawford Hall, 4249 Fifth Avenue, Pittsburgh, PA 15260, USA
    Mol Cell Biol 27:2800-11. 2007
  6. ncbi Phenotypic analysis of Paf1/RNA polymerase II complex mutations reveals connections to cell cycle regulation, protein synthesis, and lipid and nucleic acid metabolism
    J L Betz
    Department of Biology, Regis University, Denver, CO 80221, USA
    Mol Genet Genomics 268:272-85. 2002
  7. ncbi The Paf1 complex is required for histone H3 methylation by COMPASS and Dot1p: linking transcriptional elongation to histone methylation
    Nevan J Krogan
    Banting and Best Department of Medical Research, Department of Molecular and Medical Genetics, University of Toronto, Toronto, M5G 1L6, Ontario, Canada
    Mol Cell 11:721-9. 2003
  8. pmc The Paf1 complex promotes displacement of histones upon rapid induction of transcription by RNA polymerase II
    Heather A Marton
    Department of Molecular Biology and Genetics and Program in Immunology, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA
    BMC Mol Biol 9:4. 2008
  9. pmc Direct interactions between the Paf1 complex and a cleavage and polyadenylation factor are revealed by dissociation of Paf1 from RNA polymerase II
    Kristen Nordick
    Department of Biochemistry and Molecular Genetics and Molecular Biology Program, University of Colorado School of Medicine, 12801 East 17th Ave, P O Box 6511, Aurora, CO 80045, USA
    Eukaryot Cell 7:1158-67. 2008
  10. pmc Molecular evidence indicating that the yeast PAF complex is required for transcription elongation
    Ana G Rondon
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avd Reina Mercedes 6, 41012 Sevilla, Spain
    EMBO Rep 5:47-53. 2004

Research Grants

  1. TRANSCRIPTION FACTOR MUTANTS OF YEAST
    Karen Arndt; Fiscal Year: 2007
  2. Role of the Snf1 protein kinase in regulating eukaryotic gene expression
    Karen Arndt; Fiscal Year: 2007
  3. TRANSCRIPTION FACTOR MUTANTS OF YEAST
    Karen M Arndt; Fiscal Year: 2010
  4. TRANSCRIPTION FACTOR MUTANTS OF YEAST
    Karen Arndt; Fiscal Year: 2004
  5. TRANSCRIPTION FACTOR MUTANTS OF YEAST
    Karen Arndt; Fiscal Year: 2004
  6. TRANSCRIPTION FACTOR MUTANTS OF YEAST
    Karen Arndt; Fiscal Year: 1999
  7. TRANSCRIPTION FACTOR MUTANTS OF YEAST
    Karen M Arndt; Fiscal Year: 2011
  8. Biochemical analysis of Pol II elongation complexes and mechanism
    CRAIG KAPLAN; Fiscal Year: 2007

Scientific Experts

  • Karen M Arndt
  • Amy D Mozdy
  • Heather A Marton
  • Craig D Kaplan
  • C Koch
  • Elia M Crisucci
  • Judith A Jaehning
  • Stephanie E Porter
  • Yinfeng Zhang
  • Hongfang Qiu
  • Marcie H Warner
  • Manasi K Mayekar
  • Cherie L Mueller
  • Matthew G Hoffman
  • Munshi Azad Hossain
  • Brett N Tomson
  • Kristi L Penheiter
  • Richard G Gardner
  • Christopher P Davis
  • Anthony S Piro
  • Aurelie Massoni-Laporte
  • Christopher G Amrich
  • Justin A Pruneski
  • Jennifer R Stevens
  • Edel M Hyland
  • Ki Young Kim
  • Danielle Tatum
  • John A Latham
  • Nevan J Krogan
  • JESSICA L DERMODY
  • David A Schneider
  • Adam Wood
  • Cuihua Hu
  • Alan G Hinnebusch
  • Hélène Gaillard
  • Jaehoon Kim
  • Lisa A Strawn
  • M Chang
  • Kristen Nordick
  • Yaya Chu
  • Mary A Braun
  • J A Jaehning
  • Andres Aguilera
  • Carine Ganem
  • Amine Nourani
  • Markus Proft
  • John E Mueller
  • Kathryn E Sheldon
  • R Nicholas Laribee
  • Rajna Simic
  • Patrick J Costa
  • Ana G Rondon
  • Christopher D Carvin
  • Jack F Greenblatt
  • Taylor M Washburn
  • Jim Dover
  • Ali Shilatifard
  • Jessica Schneider
  • Mark Johnston
  • Dmitry K Pokholok
  • J L Betz
  • Sharon L Squazzo
  • Tracy L Johnson
  • Marinella Gebbia
  • Lawrence E Heisler
  • Christina Chung
  • Corey Nislow
  • Suman K Pradhan
  • Margaret K Shirra
  • Andrew P VanDemark
  • Annie Heroux
  • Naseem A Gaur
  • Loic Ponger
  • Anne Laure Guieysse-Peugeot
  • Helian Boucherie
  • Walter P Rogal
  • Michel Perrot
  • Sharon Y R Dent
  • Zhi Xie
  • David T Auble
  • Shisheng Li
  • David E Levin
  • Christine A Barnes
  • Shanzhi Wang
  • Kunal Poorey
  • Margaret Placer
  • Jef D Boeke
  • Troy E Perry
  • Richard A Singer
  • Joseph A Martens

Detail Information

Publications52

  1. ncbi A novel collection of accessory factors associated with yeast RNA polymerase II
    P A Wade
    Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver, Colorado, 80262, USA
    Protein Expr Purif 8:85-90. 1996
    ..Mutations in CDC73 and PAF1 affect cell growth and the abundance of transcripts from a subset of yeast genes (X. Shi et al., Mol. Cell. Biol...
  2. ncbi Kinase Cak1 functionally interacts with the PAF1 complex and phosphatase Ssu72 via kinases Ctk1 and Bur1
    Carine Ganem
    Institut Curie, UMR2027 CNRS, Centre Universitaire, Orsay, France
    Mol Genet Genomics 275:136-47. 2006
    ..Using genetic methods, we found that Cak1 genetically interacts with Paf1 and Ctr9, two components belonging to the PAF1 elongation complex needed for histone modifications, and with Ssu72, ..
  3. ncbi The stress-activated Hog1 kinase is a selective transcriptional elongation factor for genes responding to osmotic stress
    Markus Proft
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Mol Cell 23:241-50. 2006
    ..Thus, in addition to its various functions during transcriptional initiation, Hog1 behaves as a transcriptional elongation factor that is selective for genes induced upon osmotic stress...
  4. ncbi The Paf1 complex is essential for histone monoubiquitination by the Rad6-Bre1 complex, which signals for histone methylation by COMPASS and Dot1p
    Adam Wood
    Department of Biochemistry, Saint Louis University School of Medicine, St Louis, Missouri 63104, USA
    J Biol Chem 278:34739-42. 2003
    ..The Paf1 protein complex, which associates with RNA polymerase II, is known to be required for these histone H3 methylation ..
  5. pmc Identification of Rkr1, a nuclear RING domain protein with functional connections to chromatin modification in Saccharomyces cerevisiae
    Mary A Braun
    Department of Biological Sciences, University of Pittsburgh, 269 Crawford Hall, 4249 Fifth Avenue, Pittsburgh, PA 15260, USA
    Mol Cell Biol 27:2800-11. 2007
    Proper transcription by RNA polymerase II is dependent on the modification state of the chromatin template. The Paf1 complex is associated with RNA polymerase II during transcription elongation and is required for several histone ..
  6. ncbi Phenotypic analysis of Paf1/RNA polymerase II complex mutations reveals connections to cell cycle regulation, protein synthesis, and lipid and nucleic acid metabolism
    J L Betz
    Department of Biology, Regis University, Denver, CO 80221, USA
    Mol Genet Genomics 268:272-85. 2002
    b>Paf1 is an RNA polymerase II-associated protein in yeast, which defines a complex that is distinct from the Srb/Mediator holoenzyme...
  7. ncbi The Paf1 complex is required for histone H3 methylation by COMPASS and Dot1p: linking transcriptional elongation to histone methylation
    Nevan J Krogan
    Banting and Best Department of Medical Research, Department of Molecular and Medical Genetics, University of Toronto, Toronto, M5G 1L6, Ontario, Canada
    Mol Cell 11:721-9. 2003
    ..We report that the Paf1 protein complex, which is associated with the elongating RNA polymerase II, is required for methylation of lysines ..
  8. pmc The Paf1 complex promotes displacement of histones upon rapid induction of transcription by RNA polymerase II
    Heather A Marton
    Department of Molecular Biology and Genetics and Program in Immunology, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA
    BMC Mol Biol 9:4. 2008
    The yeast Paf1 protein complex is required for efficient transcription elongation by RNA polymerase II (RNA pol II), but the precise role of the complex has been unclear.
  9. pmc Direct interactions between the Paf1 complex and a cleavage and polyadenylation factor are revealed by dissociation of Paf1 from RNA polymerase II
    Kristen Nordick
    Department of Biochemistry and Molecular Genetics and Molecular Biology Program, University of Colorado School of Medicine, 12801 East 17th Ave, P O Box 6511, Aurora, CO 80045, USA
    Eukaryot Cell 7:1158-67. 2008
    The Paf1 complex (Paf1, Ctr9, Cdc73, Rtf1, and Leo1) is normally associated with RNA polymerase II (Pol II) throughout the transcription cycle...
  10. pmc Molecular evidence indicating that the yeast PAF complex is required for transcription elongation
    Ana G Rondon
    Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, Avd Reina Mercedes 6, 41012 Sevilla, Spain
    EMBO Rep 5:47-53. 2004
    PAF is a five-subunit protein complex composed of Paf1, Cdc73, Leo1, Rtf1 and Ctr9, which was purified from yeast in association with RNA polymerase II and which is believed to function in transcription elongation...
  11. pmc The Spt4p subunit of yeast DSIF stimulates association of the Paf1 complex with elongating RNA polymerase II
    Hongfang Qiu
    Laboratory of Gene Regulation and Development, National Institute of Child Health and Human Development, National Institutes of Health, Bldg 6A Rm B1A 13, Bethesda, MD 20892, USA
    Mol Cell Biol 26:3135-48. 2006
    The Paf1 complex (Paf1C) interacts with RNA polymerase II (Pol II) and promotes histone methylation of transcribed coding sequences, but the mechanism of Paf1C recruitment is unknown...
  12. pmc Regulation of histone modification and cryptic transcription by the Bur1 and Paf1 complexes
    Yaya Chu
    Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    EMBO J 26:4646-56. 2007
    The Bur1-Bur2 and Paf1 complexes function during transcription elongation and affect histone modifications. Here we describe new roles for Bur1-Bur2 and the Paf1 complex...
  13. pmc A Requirement for the Saccharomyces cerevisiae Paf1 complex in snoRNA 3' end formation
    Kathryn E Sheldon
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Mol Cell 20:225-36. 2005
    ..The yeast Paf1 complex interacts with RNA pol II and mediates histone modifications during elongation...
  14. ncbi Exchange of RNA polymerase II initiation and elongation factors during gene expression in vivo
    Dmitry K Pokholok
    Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA
    Mol Cell 9:799-809. 2002
    ..RNA polymerase II, TFIIS, Spt5, and, unexpectedly, the Paf1/Cdc73 complex, were found associated with open reading frames...
  15. pmc The Paf1 complex physically and functionally associates with transcription elongation factors in vivo
    Sharon L Squazzo
    Department of MCD Biology, University of California, Santa Cruz, CA 95064, USA
    EMBO J 21:1764-74. 2002
    ..we show that Rtf1 and Leo1, a protein of unknown function, are members of the RNA polymerase II-associated Paf1 complex...
  16. pmc Cdc73p and Paf1p are found in a novel RNA polymerase II-containing complex distinct from the Srbp-containing holoenzyme
    X Shi
    Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver 80262, USA
    Mol Cell Biol 17:1160-9. 1997
    The products of the yeast CDC73 and PAF1 genes were originally identified as RNA polymerase II-associated proteins. Paf1p is a nuclear protein important for cell growth and transcriptional regulation of a subset of yeast genes...
  17. ncbi A posttranscriptional role for the yeast Paf1-RNA polymerase II complex is revealed by identification of primary targets
    Kristi L Penheiter
    Department of Biochemistry and Molecular Genetics and Molecular Biology Program, University of Colorado, Denver, USA
    Mol Cell 20:213-23. 2005
    The yeast Paf1 complex (Paf1C: Paf1, Cdc73, Ctr9, Rtf1, and Leo1) is associated with RNA Polymerase II (Pol II) at promoters and coding regions of transcriptionally active genes, but transcript abundance for only a small subset of genes ..
  18. pmc Ctr9, Rtf1, and Leo1 are components of the Paf1/RNA polymerase II complex
    Cherie L Mueller
    Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    Mol Cell Biol 22:1971-80. 2002
    The Saccharomyces cerevisiae Paf1-RNA polymerase II (Pol II) complex is biochemically and functionally distinct from the Srb-mediator form of Pol II holoenzyme and is required for full expression of a subset of genes...
  19. pmc A complex containing RNA polymerase II, Paf1p, Cdc73p, Hpr1p, and Ccr4p plays a role in protein kinase C signaling
    M Chang
    Department of Biochemistry and Molecular Genetics and Program in Molecular Biology, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    Mol Cell Biol 19:1056-67. 1999
    ..Additionally, as previously observed for hpr1Delta, deleting PAF1 or CDC73 leads to elevated recombination between direct repeats...
  20. pmc Cdc73 subunit of Paf1 complex contains C-terminal Ras-like domain that promotes association of Paf1 complex with chromatin
    Christopher G Amrich
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Biol Chem 287:10863-75. 2012
    The conserved Paf1 complex localizes to the coding regions of genes and facilitates multiple processes during transcription elongation, including the regulation of histone modifications...
  21. pmc The yeast cap binding complex modulates transcription factor recruitment and establishes proper histone H3K36 trimethylation during active transcription
    Munshi Azad Hossain
    University of California, San Diego, Division of Biological Sciences, Molecular Biology Section, La Jolla, California, USA
    Mol Cell Biol 33:785-99. 2013
    ..This work provides evidence for an emerging model that RNA processing factors can modulate the recruitment of transcription factors and influence histone modification during elongation...
  22. pmc Effects of the Paf1 complex and histone modifications on snoRNA 3'-end formation reveal broad and locus-specific regulation
    Brett N Tomson
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Mol Cell Biol 33:170-82. 2013
    Across diverse eukaryotes, the Paf1 complex (Paf1C) plays critical roles in RNA polymerase II transcription elongation and regulation of histone modifications...
  23. pmc Pol II CTD kinases Bur1 and Kin28 promote Spt5 CTR-independent recruitment of Paf1 complex
    Hongfang Qiu
    Laboratory of Gene Regulation and Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, MD, USA
    EMBO J 31:3494-505. 2012
    b>Paf1 complex (Paf1C) is a transcription elongation factor whose recruitment is stimulated by Spt5 and the CDKs Kin28 and Bur1, which phosphorylate the Pol II C-terminal domain (CTD) on Serines 2, 5, and 7...
  24. pmc The RNA polymerase-associated factor 1 complex (Paf1C) directly increases the elongation rate of RNA polymerase I and is required for efficient regulation of rRNA synthesis
    Yinfeng Zhang
    Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama 35294 0024, USA
    J Biol Chem 285:14152-9. 2010
    ..These studies demonstrate that Paf1C plays an important direct role in cellular control of rRNA expression...
  25. pmc The recruitment of the Saccharomyces cerevisiae Paf1 complex to active genes requires a domain of Rtf1 that directly interacts with the Spt4-Spt5 complex
    Manasi K Mayekar
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Mol Cell Biol 33:3259-73. 2013
    ..One such factor is the conserved Paf1 complex (Paf1C), which regulates gene expression through several mechanisms, including the stimulation of ..
  26. pmc The Paf1 complex represses SER3 transcription in Saccharomyces cerevisiae by facilitating intergenic transcription-dependent nucleosome occupancy of the SER3 promoter
    Justin A Pruneski
    Department of Biological Sciences, University of Pittsburgh, A314 Langley Hall, 4249 Fifth Avenue, Pittsburgh, PA 15260, USA
    Eukaryot Cell 10:1283-94. 2011
    ..In this study, we uncover a role for the Paf1 transcription elongation complex in SER3 repression...
  27. pmc Paf1 restricts Gcn4 occupancy and antisense transcription at the ARG1 promoter
    Elia M Crisucci
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, USA
    Mol Cell Biol 32:1150-63. 2012
    The conserved Paf1 complex negatively regulates the expression of numerous genes, yet the mechanisms by which it represses gene expression are not well understood...
  28. pmc Small region of Rtf1 protein can substitute for complete Paf1 complex in facilitating global histone H2B ubiquitylation in yeast
    Anthony S Piro
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Proc Natl Acad Sci U S A 109:10837-42. 2012
    ..The conserved Paf1 complex (Paf1C) promotes specific histone modifications during transcription elongation, but the mechanisms by ..
  29. doi Proteome analysis of a CTR9 deficient yeast strain suggests that Ctr9 has function(s) independent of the Paf1 complex
    Aurelie Massoni-Laporte
    UMR CNRS 5095, Bordeaux, France
    Biochim Biophys Acta 1824:759-68. 2012
    The Ctr9 protein is a member of the Paf1 complex implicated in multiple functions: transcription initiation and elongation by RNA pol II, RNA processing and histone modifications...
  30. pmc Diverse roles of RNA polymerase II-associated factor 1 complex in different subpathways of nucleotide excision repair
    Danielle Tatum
    Department of Comparative Biomedical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, Louisiana 70803, USA
    J Biol Chem 286:30304-13. 2011
    ..To our best knowledge, among the NER-modulating factors documented so far, Paf1C appears to have the most diverse functions in different NER pathways or subpathways...
  31. ncbi Interaction between transcription elongation factors and mRNA 3'-end formation at the Saccharomyces cerevisiae GAL10-GAL7 locus
    Craig D Kaplan
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 280:913-22. 2005
    ..analysis suggests that Spt6 is required for cotranscriptional association of the factor Ctr9, a member of the Paf1 complex, with GAL10 and GAL7, and that Ctr9 association with chromatin 3' of GAL10 is regulated by the GAL10 ..
  32. pmc Chromatin signaling to kinetochores: transregulation of Dam1 methylation by histone H2B ubiquitination
    John A Latham
    Program in Genes and Development, University of Texas M D Anderson Cancer Center, Smithville, TX 78957, USA
    Cell 146:709-19. 2011
    ..Deletion of RAD6, BRE1, or Paf1 complex members abolishes Dam1 methylation, as does mutation of H2BK123...
  33. pmc FACT, the Bur kinase pathway, and the histone co-repressor HirC have overlapping nucleosome-related roles in yeast transcription elongation
    Jennifer R Stevens
    Department of Biochemistry and Molecular Biology, Dalhousie University, Halifax, Nova Scotia, Canada
    PLoS ONE 6:e25644. 2011
    ....
  34. pmc Effectors of lysine 4 methylation of histone H3 in Saccharomyces cerevisiae are negative regulators of PHO5 and GAL1-10
    Christopher D Carvin
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, Texas 77843 2128, USA
    J Biol Chem 279:33057-62. 2004
    ..Set1, a component of the Saccharomyces cerevisiae COMPASS complex, is regulated by the transcriptional elongation Paf1-Rtf1 and histone ubiquitination Rad6-Bre1 complexes, which are required for the expression of a subset of genes...
  35. pmc Rtf1 is a multifunctional component of the Paf1 complex that regulates gene expression by directing cotranscriptional histone modification
    Marcie H Warner
    Department of Biological Sciences, University of Pittsburgh, 269 Crawford Hall, 4249 Fifth Avenue, Pittsburgh, PA 15260, USA
    Mol Cell Biol 27:6103-15. 2007
    ..The Saccharomyces cerevisiae Paf1 complex colocalizes with actively transcribing Pol II and orchestrates modifications to the chromatin template ..
  36. ncbi The Paf1 complex has functions independent of actively transcribing RNA polymerase II
    Cherie L Mueller
    Department of Biochemistry and Molecular Genetics, Molecular Biology Program, University of Colorado Health Science Center, B121, 4200 East Ninth Avenue, Denver, CO 80262, USA
    Mol Cell 14:447-56. 2004
    The yeast Paf1 complex, minimally composed of Paf1, Ctr9, Cdc73, Rtf1, and Leo1, was originally isolated in association with RNA polymerase II (Pol II)...
  37. pmc The yeast pafl-rNA polymerase II complex is required for full expression of a subset of cell cycle-regulated genes
    Stephanie E Porter
    Department of Biochemistry and Molecular Genetics and Molecular Biology Program, University of Colorado Health Sciences Center, Denver, Colorado 80262, USA
    Eukaryot Cell 1:830-42. 2002
    ..The Pafl-RNA polymerase II complex (Paf1 complex) acts in the same pathway as the Pkc1-mitogen-activated protein kinase cascade and is required for full ..
  38. ncbi The essential function of Not1 lies within the Ccr4-Not complex
    L Maillet
    Departement de Biochimie Medicale, Centre Medical Universitaire, 1211, Geneva 4, Switzerland
    J Mol Biol 303:131-43. 2000
    ..These results are consistent with the essential function of Not1p lying within the Ccr4-Not complex...
  39. pmc A role for Ctr9p and Paf1p in the regulation G1 cyclin expression in yeast
    C Koch
    Institut für Genetik der Universität München, Maria Ward Strasse 1a, D 80638 München, Germany
    Nucleic Acids Res 27:2126-34. 1999
    ..Paf1p and Cdc73p were previously reported to be RNA poly-merase II-associated proteins, suggesting that the Ctr9p complex may interact with the general transcription apparatus...
  40. pmc Separation of the Saccharomyces cerevisiae Paf1 complex from RNA polymerase II results in changes in its subnuclear localization
    Stephanie E Porter
    Department of Biochemistry and Molecular Genetics, Molecular Biology Program, University of Colorado Health Sciences Center, Denver, CO 80262, USA
    Eukaryot Cell 4:209-20. 2005
    The yeast Paf1 complex (Paf1C), composed of Paf1, Ctr9, Cdc73, Rtf1, and Leo1, associates with RNA polymerase II (Pol II) at promoters and in the actively transcribed portions of mRNA genes...
  41. pmc The Paf1 complex represses ARG1 transcription in Saccharomyces cerevisiae by promoting histone modifications
    Elia M Crisucci
    Department of Biological Sciences, University of Pittsburgh, A316 Langley Hall, 4249 Fifth Ave, Pittsburgh, PA 15260, USA
    Eukaryot Cell 10:712-23. 2011
    The conserved multifunctional Paf1 complex is important for the proper transcription of numerous genes, and yet the exact mechanisms by which it controls gene expression remain unclear...
  42. pmc The requirements for COMPASS and Paf1 in transcriptional silencing and methylation of histone H3 in Saccharomyces cerevisiae
    John E Mueller
    Department of Biochemistry and Biophysics, Texas A and M University, College Station, Texas 77843 2128, USA
    Genetics 173:557-67. 2006
    ..Finally, we show Paf1 is required for silencing and K4-methylated H3 at the rDNA, suggesting a possible direct role for K4-methylated H3 ..
  43. pmc Evidence that Spt2/Sin1, an HMG-like factor, plays roles in transcription elongation, chromatin structure, and genome stability in Saccharomyces cerevisiae
    Amine Nourani
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, MA 02115, USA
    Mol Cell Biol 26:1496-509. 2006
    ..Taken together, our results suggest a direct link for Spt2 with transcription elongation, chromatin dynamics, and genome stability...
  44. pmc Multiple yeast genes, including Paf1 complex genes, affect telomere length via telomerase RNA abundance
    Amy D Mozdy
    Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Mol Cell Biol 28:4152-61. 2008
    ..This report establishes that a subset of telomere length genes act by modulating telomerase RNA abundance...
  45. pmc The Paf1 complex is required for efficient transcription elongation by RNA polymerase I
    Yinfeng Zhang
    Department of Biochemistry and Molecular Genetics, University of Alabama, Birmingham, AL 35294 0024, USA
    Proc Natl Acad Sci U S A 106:2153-8. 2009
    ..To date, the factors involved in the control of Pol I transcription elongation are poorly understood. The Paf1 complex (Paf1C) is a well-defined factor that influences polymerase II (Pol II) transcription elongation...
  46. pmc Genome-wide analysis of factors affecting transcription elongation and DNA repair: a new role for PAF and Ccr4-not in transcription-coupled repair
    Hélène Gaillard
    Centro Andaluz de Biologia Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla CSIC, Sevilla, Spain
    PLoS Genet 5:e1000364. 2009
    ....
  47. pmc Mutants of the Paf1 complex alter phenotypic expression of the yeast prion [PSI+]
    Lisa A Strawn
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Biol Cell 20:2229-41. 2009
    ..We have identified one such global regulator, the Paf1 complex (Paf1C)...
  48. pmc Direct Bre1-Paf1 complex interactions and RING finger-independent Bre1-Rad6 interactions mediate histone H2B ubiquitylation in yeast
    Jaehoon Kim
    Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, New York 10065, USA
    J Biol Chem 284:20582-92. 2009
    ..These studies provide a more detailed mechanistic basis for H2B ubiquitylation in yeast...
  49. ncbi BUR kinase selectively regulates H3 K4 trimethylation and H2B ubiquitylation through recruitment of the PAF elongation complex
    R Nicholas Laribee
    Department of Biochemistry and Biophysics, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27599, USA
    Curr Biol 15:1487-93. 2005
    ..Our data reveal a novel function for the BUR kinase in transcriptional regulation through the selective control of histone modifications...
  50. pmc Leo1 subunit of the yeast paf1 complex binds RNA and contributes to complex recruitment
    JESSICA L DERMODY
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 285:33671-9. 2010
    The Paf1 complex (Paf1C) affects RNA polymerase II transcription by coordinating co-transcriptional chromatin modifications and helping recruit mRNA 3' end processing factors...
  51. pmc Mpk1 MAPK association with the Paf1 complex blocks Sen1-mediated premature transcription termination
    Ki Young Kim
    Department of Molecular and Cell Biology, Boston University Goldman School of Dental Medicine, Boston University, Boston, MA 02118, USA
    Cell 144:745-56. 2011
    ..This function is mediated by an interaction between Mpk1 and the Paf1 subunit of the Paf1C elongation complex...
  52. pmc An evolutionarily 'young' lysine residue in histone H3 attenuates transcriptional output in Saccharomyces cerevisiae
    Edel M Hyland
    High Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA
    Genes Dev 25:1306-19. 2011
    ..Interestingly, this site of methylation is unique to Ascomycota, suggesting a recent evolutionary innovation that highlights the evolvability of post-translational modifications of chromatin...

Research Grants21

  1. TRANSCRIPTION FACTOR MUTANTS OF YEAST
    Karen Arndt; Fiscal Year: 2007
    ..focuses on transcription elongation and a recently discovered elongation factor in Saccharomyces cerevisiae, the Paf1 complex...
  2. Role of the Snf1 protein kinase in regulating eukaryotic gene expression
    Karen Arndt; Fiscal Year: 2007
    ..The conservation between Snf1 and AMPK and between their potential targets strongly suggests that the proposed studies will provide new insights into the function of AMPK. ..
  3. TRANSCRIPTION FACTOR MUTANTS OF YEAST
    Karen M Arndt; Fiscal Year: 2010
    ..Specific Aims 1 and 2 focus on the conserved Paf1 complex, which interacts with RNA pol II and couples chromatin changes to transcription elongation...
  4. TRANSCRIPTION FACTOR MUTANTS OF YEAST
    Karen Arndt; Fiscal Year: 2004
    ....
  5. TRANSCRIPTION FACTOR MUTANTS OF YEAST
    Karen Arndt; Fiscal Year: 2004
    ..abstract_text> ..
  6. TRANSCRIPTION FACTOR MUTANTS OF YEAST
    Karen Arndt; Fiscal Year: 1999
    ....
  7. TRANSCRIPTION FACTOR MUTANTS OF YEAST
    Karen M Arndt; Fiscal Year: 2011
    ..Specific Aims 1 and 2 focus on the conserved Paf1 complex, which interacts with RNA pol II and couples chromatin changes to transcription elongation...
  8. Biochemical analysis of Pol II elongation complexes and mechanism
    CRAIG KAPLAN; Fiscal Year: 2007
    ..Insights into Pol II function will allow a better understanding of how Pol II may be misregulated in disease states in other species, such as humans. ..