PAC10

Summary

Gene Symbol: PAC10
Description: tubulin-binding prefolding complex subunit PAC10
Alias: GIM2, PFD3, RKS2, tubulin-binding prefolding complex subunit PAC10
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Lacefield S, Solomon F. A novel step in beta-tubulin folding is important for heterodimer formation in Saccharomyces cerevisiae. Genetics. 2003;165:531-41 pubmed
    ..As a result, plp1Delta cells have lower levels of heterodimer. Importantly, plp1Delta cells that also lack Pac10, a component of the GimC/PFD complex, are even less affected by free beta-tubulin...
  2. Mayer M, Pot I, Chang M, Xu H, Aneliunas V, Kwok T, et al. Identification of protein complexes required for efficient sister chromatid cohesion. Mol Biol Cell. 2004;15:1736-45 pubmed
    ..Furthermore, we find that genes involved in mitotic spindle integrity and positioning have a previously unrecognized role in sister chromatid cohesion. ..
  3. Lacefield S, Magendantz M, Solomon F. Consequences of defective tubulin folding on heterodimer levels, mitosis and spindle morphology in Saccharomyces cerevisiae. Genetics. 2006;173:635-46 pubmed
  4. Geissler S, Siegers K, Schiebel E. A novel protein complex promoting formation of functional alpha- and gamma-tubulin. EMBO J. 1998;17:952-66 pubmed
    We describe the identification of GIM1/YKE2, GIM2/PAC10, GIM3, GIM4 and GIM5 in a screen for mutants that are synthetically lethal with tub4-1, encoding a mutated yeast gamma-tubulin...
  5. Alvarez P, Smith A, Fleming J, Solomon F. Modulation of tubulin polypeptide ratios by the yeast protein Pac10p. Genetics. 1998;149:857-64 pubmed
    ..Cells deleted for PAC10 have the same phenotypes as do cells that express reduced levels of alpha-tubulin or Rbl2p, two proteins that bind ..
  6. Abruzzi K, Smith A, Chen W, Solomon F. Protection from free beta-tubulin by the beta-tubulin binding protein Rbl2p. Mol Cell Biol. 2002;22:138-47 pubmed
    ..Rescue of beta-tubulin toxicity requires a minimal but substoichiometric ratio of Rbl2p to beta-tubulin. The data suggest that Rbl2p binds transiently to free beta-tubulin, which then passes into an aggregated form that is not toxic. ..
  7. Brew C, Huffaker T. The yeast ubiquitin protease, Ubp3p, promotes protein stability. Genetics. 2002;162:1079-89 pubmed
    ..Overall, these data support the idea that Ubp3p has a general role in the reversal of protein ubiquitination. ..
  8. Ragni E, Piberger H, Neupert C, García Cantalejo J, Popolo L, Arroyo J, et al. The genetic interaction network of CCW12, a Saccharomyces cerevisiae gene required for cell wall integrity during budding and formation of mating projections. BMC Genomics. 2011;12:107 pubmed publisher
    ..Among those are PFD1, WHI3, SRN2, PAC10, FEN1 and YDR417C, which have not been related to cell wall integrity before...
  9. Vainberg I, Lewis S, Rommelaere H, Ampe C, Vandekerckhove J, Klein H, et al. Prefoldin, a chaperone that delivers unfolded proteins to cytosolic chaperonin. Cell. 1998;93:863-73 pubmed
    ..We show that by directing target proteins to chaperonin, prefoldin promotes folding in an environment in which there are many competing pathways for nonnative proteins. ..

More Information

Publications11

  1. Albanèse V, Yam A, Baughman J, Parnot C, Frydman J. Systems analyses reveal two chaperone networks with distinct functions in eukaryotic cells. Cell. 2006;124:75-88 pubmed
    ..The emergence of a translation-linked chaperone network likely underlies the elaborate cotranslational folding process necessary for the evolution of larger multidomain proteins characteristic of eukaryotic cells. ..
  2. Scarfone I, Venturetti M, Hotz M, Lengefeld J, Barral Y, Piatti S. Asymmetry of the budding yeast Tem1 GTPase at spindle poles is required for spindle positioning but not for mitotic exit. PLoS Genet. 2015;11:e1004938 pubmed publisher
    ..Thus, asymmetry of the Bub2/Bfa1/Tem1 complex is crucial to control Kar9 distribution and spindle positioning during mitosis. ..