ORM2

Summary

Gene Symbol: ORM2
Description: sphingolipid homeostasis protein ORM2
Alias: sphingolipid homeostasis protein ORM2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Breslow D, Collins S, Bodenmiller B, Aebersold R, Simons K, Shevchenko A, et al. Orm family proteins mediate sphingolipid homeostasis. Nature. 2010;463:1048-53 pubmed publisher
    ..Our work identifies the Orm proteins as critical mediators of sphingolipid homeostasis and raises the possibility that sphingolipid misregulation contributes to the development of childhood asthma. ..
  2. Han S, Lone M, Schneiter R, Chang A. Orm1 and Orm2 are conserved endoplasmic reticulum membrane proteins regulating lipid homeostasis and protein quality control. Proc Natl Acad Sci U S A. 2010;107:5851-6 pubmed publisher
    ..In the absence of yeast Orm1 and Orm2, accumulation of long chain base, a sphingolipid precursor, suggests dysregulation of sphingolipid synthesis...
  3. Roelants F, Breslow D, Muir A, Weissman J, Thorner J. Protein kinase Ypk1 phosphorylates regulatory proteins Orm1 and Orm2 to control sphingolipid homeostasis in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2011;108:19222-7 pubmed publisher
    ..In Saccharomyces cerevisiae, Orm1 and Orm2 are inactivated by phosphorylation in response to compromised sphingolipid synthesis (e.g...
  4. Sun Y, Miao Y, Yamane Y, Zhang C, Shokat K, Takematsu H, et al. Orm protein phosphoregulation mediates transient sphingolipid biosynthesis response to heat stress via the Pkh-Ypk and Cdc55-PP2A pathways. Mol Biol Cell. 2012;23:2388-98 pubmed publisher
    ..We propose that this mechanism serves as the basis for how Orm phosphoregulation controls sphingolipid biosynthesis in response to stress in a kinetically coupled manner. ..
  5. Toume M, Tani M. Yeast lacking the amphiphysin family protein Rvs167 is sensitive to disruptions in sphingolipid levels. FEBS J. 2016;283:2911-28 pubmed publisher
    ..In rvs167? cells, the expression level of Orm2, a negative regulator of serine palmitoyltransferase (SPT) catalyzing the initial step of sphingolipid biosynthesis,..
  6. Lindahl L, Santos A, Olsson H, Olsson L, Bettiga M. Membrane engineering of S. cerevisiae targeting sphingolipid metabolism. Sci Rep. 2017;7:41868 pubmed publisher
    ..Deletion of the genes ORM1 and ORM2, encoding negative regulators of sphingolipid biosynthesis, decreased cell viability and considerably reduced ..
  7. Martínez Montañés F, Lone M, Hsu F, Schneiter R. Accumulation of long-chain bases in yeast promotes their conversion to a long-chain base vinyl ether. J Lipid Res. 2016;57:2040-2050 pubmed
    ..Consistent with a role of PHS vinyl ether formation in LCB detoxification, the lipid is efficiently exported from the cells. ..
  8. Rispal D, Eltschinger S, Stahl M, Vaga S, Bodenmiller B, Abraham Y, et al. Target of Rapamycin Complex 2 Regulates Actin Polarization and Endocytosis via Multiple Pathways. J Biol Chem. 2015;290:14963-78 pubmed publisher
    ..Additional hits in our phosphoproteomic screens highlight the intricate control TORC2 exerts over diverse aspects of eukaryote cell physiology. ..
  9. Surlow B, Cooley B, Needham P, Brodsky J, Patton Vogt J. Loss of Ypk1, the yeast homolog to the human serum- and glucocorticoid-induced protein kinase, accelerates phospholipase B1-mediated phosphatidylcholine deacylation. J Biol Chem. 2014;289:31591-604 pubmed publisher
    ..Taken together, these results suggest a functional interaction between Ypk1 and Plb1 in which altered sphingolipid metabolism up-regulates PLB1 expression via Crz1. ..

More Information

Publications18

  1. Toume M, Tani M. Change in activity of serine palmitoyltransferase affects sensitivity to syringomycin E in yeast Saccharomyces cerevisiae. FEMS Microbiol Lett. 2014;358:64-71 pubmed publisher
    ..found a novel mutation that confers resistance to syringomycin E on yeast; that is, a deletion mutant of ORM1 and ORM2, which encode negative regulators of serine palmitoyltransferase catalyzing the initial step of sphingolipid ..
  2. Gururaj C, Federman R, Federman R, Chang A. Orm proteins integrate multiple signals to maintain sphingolipid homeostasis. J Biol Chem. 2013;288:20453-63 pubmed publisher
    ..The recently discovered SPT-associated proteins, Orm1 and Orm2, are critical regulators of sphingolipids...
  3. Shimobayashi M, Oppliger W, Moes S, Jeno P, Hall M. TORC1-regulated protein kinase Npr1 phosphorylates Orm to stimulate complex sphingolipid synthesis. Mol Biol Cell. 2013;24:870-81 pubmed publisher
    The evolutionarily conserved Orm1 and Orm2 proteins mediate sphingolipid homeostasis. However, the homologous Orm proteins and the signaling pathways modulating their phosphorylation and function are incompletely characterized...
  4. Lester R, Withers B, Schultz M, Dickson R. Iron, glucose and intrinsic factors alter sphingolipid composition as yeast cells enter stationary phase. Biochim Biophys Acta. 2013;1831:726-36 pubmed publisher
    ..These changes are due to intrinsic factors including Orm1 and Orm2, ceramide synthase, Lcb4 kinase and the Tsc3 subunit of serine palmitoyltransferase as well as extrinsic factors ..
  5. Lee Y, Huang X, Kropat J, Henras A, Merchant S, Dickson R, et al. Sphingolipid signaling mediates iron toxicity. Cell Metab. 2012;16:90-6 pubmed publisher
    ..These results demonstrate an unexpected connection between sphingolipid flux and iron toxicity and show that activation of a signal transduction cascade contributes to iron-mediated cellular toxicity. ..
  6. Liu M, Huang C, Polu S, Schneiter R, Chang A. Regulation of sphingolipid synthesis through Orm1 and Orm2 in yeast. J Cell Sci. 2012;125:2428-35 pubmed publisher
    ..Yeast Orm1 and Orm2 belong to a conserved family of ER membrane proteins that regulate serine palmitoyltransferase, which catalyzes the ..
  7. Zimmermann C, Santos A, Gable K, Epstein S, Gururaj C, Chymkowitch P, et al. TORC1 inhibits GSK3-mediated Elo2 phosphorylation to regulate very long chain fatty acid synthesis and autophagy. Cell Rep. 2013;5:1036-46 pubmed publisher
    ..Together, our data reveal a function for TORC1 and GSK3 in the regulation of VLCFA synthesis that has important implications for autophagy and cell homeostasis. ..
  8. Chauhan N, Han G, Somashekarappa N, Gable K, Dunn T, Kohlwein S. Regulation of Sphingolipid Biosynthesis by the Morphogenesis Checkpoint Kinase Swe1. J Biol Chem. 2016;291:2524-34 pubmed publisher
  9. Hjelmqvist L, Tuson M, Marfany G, Herrero E, Balcells S, Gonzalez Duarte R. ORMDL proteins are a conserved new family of endoplasmic reticulum membrane proteins. Genome Biol. 2002;3:RESEARCH0027 pubmed
    ..Subcellular localization and the response of yeast mutants to specific agents point to the involvement of ORMDL in protein folding in the ER. ..