Gene Symbol: OPI3
Description: bifunctional phosphatidyl-N-methylethanolamine N-methyltransferase/phosphatidyl-N-dimethylethanolamine N-methyltransferase
Alias: PEM2, bifunctional phosphatidyl-N-methylethanolamine N-methyltransferase/phosphatidyl-N-dimethylethanolamine N-methyltransferase
Species: Saccharomyces cerevisiae S288c
Products:     OPI3

Top Publications

  1. Kodaki T, Yamashita S. Characterization of the methyltransferases in the yeast phosphatidylethanolamine methylation pathway by selective gene disruption. Eur J Biochem. 1989;185:243-51 pubmed
    PEM1 and PEM2 are structural genes for the yeast phosphatidylethanolamine methylation pathway which mediates the three-step methylation of phosphatidylethanolamine to phosphatidylcholine...
  2. Kodaki T, Yamashita S. Yeast phosphatidylethanolamine methylation pathway. Cloning and characterization of two distinct methyltransferase genes. J Biol Chem. 1987;262:15428-35 pubmed
    The structural genes (PEM1 and PEM2) encoding the enzymes involved in the yeast phosphatidylethanolamine (PE) methylation pathway were cloned by means of genetic complementation using yeast mutants...
  3. Thibault G, Shui G, Kim W, McAlister G, Ismail N, Gygi S, et al. The membrane stress response buffers lethal effects of lipid disequilibrium by reprogramming the protein homeostasis network. Mol Cell. 2012;48:16-27 pubmed publisher
    ..We demonstrate that the UPR maintains protein biogenesis, quality control, and membrane integrity-functions otherwise lethally compromised in lipid dysregulated cells. ..
  4. Preitschopf W, Lückl H, Summers E, Henry S, Paltauf F, Kohlwein S. Molecular cloning of the yeast OPI3 gene as a high copy number suppressor of the cho2 mutation. Curr Genet. 1993;23:95-101 pubmed
    ..the restriction pattern of the cloned gene to published sequences, unequivocally identified the cloned gene as the OPI3 gene, encoding phospholipid-N-methyltransferase in yeast...
  5. Bilgin M, Markgraf D, Duchoslav E, Knudsen J, Jensen O, de Kroon A, et al. Quantitative profiling of PE, MMPE, DMPE, and PC lipid species by multiple precursor ion scanning: a tool for monitoring PE metabolism. Biochim Biophys Acta. 2011;1811:1081-9 pubmed publisher
    ..Finally, we benchmarked the mass-tag strategy by specific and sensitive profiling of intermediate MMPE and DMPE species in liver. ..
  6. Oki M, Ma L, Wang Y, Hatanaka A, Miyazato C, Tatebayashi K, et al. Identification of novel suppressors for Mog1 implies its involvement in RNA metabolism, lipid metabolism and signal transduction. Gene. 2007;400:114-21 pubmed
    ..Furthermore, SpMog1 was co-precipitated with Nxt2 and Cid13. ..
  7. Sakakibara K, Eiyama A, Suzuki S, Sakoh Nakatogawa M, Okumura N, Tani M, et al. Phospholipid methylation controls Atg32-mediated mitophagy and Atg8 recycling. EMBO J. 2015;34:2703-19 pubmed publisher
    ..pathway for conversion of phosphatidylethanolamine to phosphatidylcholine by the two methyltransferases Cho2 and Opi3. Under mitophagy-inducing conditions, cells lacking Opi3 exhibit retardation of Cho2 repression that causes an ..
  8. Garbarino J, Padamsee M, Wilcox L, Oelkers P, D Ambrosio D, Ruggles K, et al. Sterol and diacylglycerol acyltransferase deficiency triggers fatty acid-mediated cell death. J Biol Chem. 2009;284:30994-1005 pubmed publisher
    ..In metazoans, many of these processes are implicated in insulin secretion thus linking lipotoxicity with early aspects of pancreatic beta-cell dysfunction, diabetes, and the metabolic syndrome. ..
  9. Federovitch C, Jones Y, Tong A, Boone C, Prinz W, Hampton R. Genetic and structural analysis of Hmg2p-induced endoplasmic reticulum remodeling in Saccharomyces cerevisiae. Mol Biol Cell. 2008;19:4506-20 pubmed publisher
    ..These phospholipid effects, unlike Hmg2p-induced ER remodeling, required the enzymatic activity of Hmg2p. Together, our results indicate that, although related, Hmg2p- and Hmg1p-induced ER remodeling are mechanistically distinct. ..

More Information


  1. Riekhof W, Wu J, Gijón M, Zarini S, Murphy R, Voelker D. Lysophosphatidylcholine metabolism in Saccharomyces cerevisiae: the role of P-type ATPases in transport and a broad specificity acyltransferase in acylation. J Biol Chem. 2007;282:36853-61 pubmed
    ..These results establish a new pathway for the net synthesis of PtdCho in yeast and provide new tools for the study of PtdCho synthesis, transport, and remodeling...
  2. Bidlingmaier S, Snyder M. Large-scale identification of genes important for apical growth in Saccharomyces cerevisiae by directed allele replacement technology (DART) screening. Funct Integr Genomics. 2002;1:345-56 pubmed
    ..mutations in 17 different genes ( SMY1, SPA2, PAN1, SLA1, SLA2, CBK1, SEC22, FAB1, VPS36, VID22, RAS2, ECM33, OPI3, API1/YDR372c, API2/YDR525w, API3/YKR020w, and API4/YNL051w), which alter the elongated bud morphology of cdc34-2 ..
  3. Greenberg M, Klig L, Letts V, Loewy B, Henry S. Yeast mutant defective in phosphatidylcholine synthesis. J Bacteriol. 1983;153:791-9 pubmed
    The Saccharomyces cerevisiae opi3-3 mutant was shown to be defective in the synthesis of phosphatidylcholine via methylation of phosphatidylethanolamine...
  4. Boumann H, Gubbens J, Koorengevel M, Oh C, Martin C, Heck A, et al. Depletion of phosphatidylcholine in yeast induces shortening and increased saturation of the lipid acyl chains: evidence for regulation of intrinsic membrane curvature in a eukaryote. Mol Biol Cell. 2006;17:1006-17 pubmed
    ..The shortening and increased saturation of the PE acyl chains were shown to decrease the nonbilayer propensity of PE. The results point to a regulatory mechanism in yeast that maintains intrinsic membrane curvature in an optimal range. ..
  5. BeGora M, Macleod M, McCarry B, Summers P, Weretilnyk E. Identification of phosphomethylethanolamine N-methyltransferase from Arabidopsis and its role in choline and phospholipid metabolism. J Biol Chem. 2010;285:29147-55 pubmed publisher
    ..methylation steps was cloned from Arabidopsis by heterologous complementation of a Saccharomyces cerevisiae cho2, opi3 mutant...
  6. Gulshan K, Moye Rowley W. Vacuolar import of phosphatidylcholine requires the ATP-binding cassette transporter Ybt1. Traffic. 2011;12:1257-68 pubmed publisher
    ..Our data describe a novel vacuolar route for lipid remodeling and reutilization in addition to previously described enzymatic avenues in the cytoplasm...
  7. Tavassoli S, Chao J, Young B, Cox R, Prinz W, de Kroon A, et al. Plasma membrane--endoplasmic reticulum contact sites regulate phosphatidylcholine synthesis. EMBO Rep. 2013;14:434-40 pubmed publisher
    ..phosphatidylcholine synthesis and regulate the activity of the phosphatidylethanolamine N-methyltransferase enzyme, Opi3. Opi3 activity requires Osh3, which localizes to PM-ER contacts where it might facilitate in trans catalysis by ..
  8. Gaynor P, Carman G. Phosphatidylethanolamine methyltransferase and phospholipid methyltransferase activities from Saccharomyces cerevisiae. Enzymological and kinetic properties. Biochim Biophys Acta. 1990;1045:156-63 pubmed
    ..AdoHcy was a noncompetitive inhibitor of PEMT (Ki = 160 microM) and PLMT (Ki = 120 microM) with respect to PE and PMME and PDME, respectively. ..