Gene Symbol: OM45
Species: Saccharomyces cerevisiae S288c
Song J, Tamura Y, Yoshihisa T, Endo T. A novel import route for an N-anchor mitochondrial outer membrane protein aided by the TIM23 complex. EMBO Rep. 2014;15:670-7 pubmed publisher
The membrane topology of Om45 in the yeast mitochondrial outer membrane (OM) is under debate. Here, we confirm that Om45 is anchored to the OM from the intermembrane space (IMS) by its N-terminal hydrophobic segment...
Cerveny K, McCaffery J, Jensen R. Division of mitochondria requires a novel DMN1-interacting protein, Net2p. Mol Biol Cell. 2001;12:309-21 pubmed
..Our results suggest that Net2p is a new component of the mitochondrial division machinery. ..
Grandier Vazeille X, Bathany K, Chaignepain S, Camougrand N, Manon S, Schmitter J. Yeast mitochondrial dehydrogenases are associated in a supramolecular complex. Biochemistry. 2001;40:9758-69 pubmed
..The association of these proteins is discussed in terms of NADH-channeling. ..
Dunn C, Lee M, Spencer F, Jensen R. A genomewide screen for petite-negative yeast strains yields a new subunit of the i-AAA protease complex. Mol Biol Cell. 2006;17:213-26 pubmed
..Our results highlight the importance of the i-AAA complex and proteolysis at the inner membrane in cells lacking mitochondrial DNA. ..
Burri L, Vascotto K, Gentle I, Chan N, Beilharz T, Stapleton D, et al
. Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae. FEBS J. 2006;273:1507-15 pubmed
..The alpha-helical outer membrane proteins provide functions derived after the endosymbiotic event, and require the translocase in the outer mitochondrial membrane complex for insertion into the outer membrane. ..
Schmidt O, Harbauer A, Rao S, Eyrich B, Zahedi R, Stojanovski D, et al
. Regulation of mitochondrial protein import by cytosolic kinases. Cell. 2011;144:227-39 pubmed publisher
..We conclude that cytosolic kinases exert stimulatory and inhibitory effects on biogenesis and function of the TOM complex and thus regulate protein import into mitochondria. ..
Merklinger E, Gofman Y, Kedrov A, Driessen A, Ben Tal N, Shai Y, et al
. Membrane integration of a mitochondrial signal-anchored protein does not require additional proteinaceous factors. Biochem J. 2012;442:381-9 pubmed publisher
..To shed light on these issues, we employed a recombinant version of the SA protein OM45 and a synthetic peptide corresponding to its signal-anchor segment...
Lauffer S, Mäbert K, Czupalla C, Pursche T, Hoflack B, Rödel G, et al
. Saccharomyces cerevisiae porin pore forms complexes with mitochondrial outer membrane proteins Om14p and Om45p. J Biol Chem. 2012;287:17447-58 pubmed publisher
..BN)-PAGE analysis, co-immunoprecipitation, and tandem affinity purification that the two mt OM proteins Om14p and Om45p associate with the porin pore. Porin molecules seem to assemble independently to build the core unit...
Wenz L, Opaliński L, Schuler M, Ellenrieder L, Ieva R, Böttinger L, et al
. The presequence pathway is involved in protein sorting to the mitochondrial outer membrane. EMBO Rep. 2014;15:678-85 pubmed publisher
..It has been reported that the biogenesis of Om45, the most abundant signal-anchored protein, does not depend on proteinaceous components, but involves direct ..