OLE1

Summary

Gene Symbol: OLE1
Description: stearoyl-CoA 9-desaturase
Alias: MDM2, stearoyl-CoA 9-desaturase
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Garbarino J, Padamsee M, Wilcox L, Oelkers P, D Ambrosio D, Ruggles K, et al. Sterol and diacylglycerol acyltransferase deficiency triggers fatty acid-mediated cell death. J Biol Chem. 2009;284:30994-1005 pubmed publisher
    ..In metazoans, many of these processes are implicated in insulin secretion thus linking lipotoxicity with early aspects of pancreatic beta-cell dysfunction, diabetes, and the metabolic syndrome. ..
  2. Tatzer V, Zellnig G, Kohlwein S, Schneiter R. Lipid-dependent subcellular relocalization of the acyl chain desaturase in yeast. Mol Biol Cell. 2002;13:4429-42 pubmed
    ..A model is discussed in which the accumulation of saturated lipids in a microdomain around the desaturase could induce the observed segregation and relocalization of the enzyme. ..
  3. Trotter P, Hagerman R, Voelker D. A yeast strain defective in oleic acid utilization has a mutation in the RML2 gene. Biochim Biophys Acta. 1999;1438:223-38 pubmed
    ..The fat21 mutant allele displays conditional synthetic lethality in combination with a null allele of the OLE1 gene, which encodes Delta9-desaturase and is required for proper mitochondrial segregation...
  4. Stukey J, McDonough V, Martin C. The OLE1 gene of Saccharomyces cerevisiae encodes the delta 9 fatty acid desaturase and can be functionally replaced by the rat stearoyl-CoA desaturase gene. J Biol Chem. 1990;265:20144-9 pubmed
    Strains of Saccharomyces cerevisiae bearing the ole1 mutation are defective in unsaturated fatty acid (UFA) synthesis and require UFAs for growth...
  5. Kojima R, Kajiura S, Sesaki H, Endo T, Tamura Y. Identification of multi-copy suppressors for endoplasmic reticulum-mitochondria tethering proteins in Saccharomyces cerevisiae. FEBS Lett. 2016;590:3061-70 pubmed publisher
    ..Spt23 and Mga2 are homologous transcription factors known to activate transcription of the OLE1 gene, which encodes the fatty acid ?9 desaturase...
  6. Hitchcock A, Krebber H, Frietze S, Lin A, Latterich M, Silver P. The conserved npl4 protein complex mediates proteasome-dependent membrane-bound transcription factor activation. Mol Biol Cell. 2001;12:3226-41 pubmed
    ..cerevisiae proteins Mga2p and Spt23p, which direct transcription of the Delta9-fatty acid desaturase gene OLE1. We now show that a membrane-associated complex containing the highly conserved Npl4p, Ufd1p, and Cdc48p proteins ..
  7. Li X, Li Y, Arendt C, Hochstrasser M. Distinct Elements in the Proteasomal β5 Subunit Propeptide Required for Autocatalytic Processing and Proteasome Assembly. J Biol Chem. 2016;291:1991-2003 pubmed publisher
    ..Our results suggest that β7 insertion precedes half-mer dimerization, and the β7 tail and β5 propeptide have unequal roles in half-mer joining. ..
  8. Fang Z, Chen Z, Wang S, Shi P, Shen Y, Zhang Y, et al. Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae. Appl Environ Microbiol. 2017;83: pubmed
    ..A candidate gene, OLE1, encodes a delta-9 desaturase and was associated with high anti-cadmium-stress activity...
  9. Mitchell A, Martin C. A novel cytochrome b5-like domain is linked to the carboxyl terminus of the Saccharomyces cerevisiae delta-9 fatty acid desaturase. J Biol Chem. 1995;270:29766-72 pubmed
    ..does not appear to require cytochrome b5, introduction of the rat liver stearoyl-CoA desaturase gene into an ole1-disrupted, cytochrome b5-disrupted yeast strain revealed that this enzyme specifically requires cytochrome b5 to ..

More Information

Publications19

  1. Schneiter R, Tatzer V, Gogg G, Leitner E, Kohlwein S. Elo1p-dependent carboxy-terminal elongation of C14:1Delta(9) to C16:1Delta(11) fatty acids in Saccharomyces cerevisiae. J Bacteriol. 2000;182:3655-60 pubmed
    ..These results will be discussed in terms of biosynthesis of different phospholipid classes via either the de novo or the Kennedy pathway. ..
  2. Siepe D, Jentsch S. Prolyl isomerase Pin1 acts as a switch to control the degree of substrate ubiquitylation. Nat Cell Biol. 2009;11:967-72 pubmed publisher
    ..This suggests that the Pin1 activity is often translated into a fate-determining ubiquitylation switch, and that Pin1 may affect the degree of substrate ubiquitylation in other pathways as well. ..
  3. Ballweg S, Ernst R. Control of membrane fluidity: the OLE pathway in focus. Biol Chem. 2017;398:215-228 pubmed publisher
    ..of the transcription factors Mga2 and Spt23 from the endoplasmic reticulum (ER), which then drive the expression of OLE1 in the nucleus...
  4. Nasution O, Lee Y, Kim E, Lee Y, Kim W, Choi W. Overexpression of OLE1 enhances stress tolerance and constitutively activates the MAPK HOG pathway in Saccharomyces cerevisiae. Biotechnol Bioeng. 2017;114:620-631 pubmed publisher
    b>OLE1 of Saccharomyces cerevisiae encodes the sole and essential ?-9 desaturase catalyzing the conversion of saturated to unsaturated fatty acids. Upon ectopic overexpression of OLE1 in S...
  5. De Smet C, Vittone E, Scherer M, Houweling M, Liebisch G, Brouwers J, et al. The yeast acyltransferase Sct1p regulates fatty acid desaturation by competing with the desaturase Ole1p. Mol Biol Cell. 2012;23:1146-56 pubmed publisher
    ..The level of Sct1p phosphorylation is increased when cells are supplemented with saturated fatty acids, demonstrating the physiological relevance of our findings. ..
  6. Jacquier N, Schneiter R. Ypk1, the yeast orthologue of the human serum- and glucocorticoid-induced kinase, is required for efficient uptake of fatty acids. J Cell Sci. 2010;123:2218-27 pubmed publisher
    ..Consistent with such a proposition, mutations that block early steps of endocytosis display reduced levels of fatty-acid uptake. ..
  7. McConnell S, Stewart L, Talin A, Yaffe M. Temperature-sensitive yeast mutants defective in mitochondrial inheritance. J Cell Biol. 1990;111:967-76 pubmed
    ..Two of these mutants contain single, recessive, nuclear mutations, mdm1 and mdm2, that cause temperature-sensitive growth and aberrant mitochondrial distribution at the nonpermissive temperature...
  8. Lou Y, Shanklin J. Evidence that the yeast desaturase Ole1p exists as a dimer in vivo. J Biol Chem. 2010;285:19384-90 pubmed publisher
  9. Yahara N, Sato K, Nakano A. The Arf1p GTPase-activating protein Glo3p executes its regulatory function through a conserved repeat motif at its C-terminus. J Cell Sci. 2006;119:2604-12 pubmed
    ..We name this region the Glo3 motif and present evidence that the motif is required for the function of Glo3p in vivo. ..
  10. Zhang S, Skalsky Y, Garfinkel D. MGA2 or SPT23 is required for transcription of the delta9 fatty acid desaturase gene, OLE1, and nuclear membrane integrity in Saccharomyces cerevisiae. Genetics. 1999;151:473-83 pubmed
    ..We have isolated a gene-specific activator, SWI5, and the Delta9 fatty acid desaturase of yeast, OLE1, as multicopy suppressors of an mga2Delta spt23 temperature-sensitive mutation (spt23-ts)...