NUP84

Summary

Gene Symbol: NUP84
Description: Nup84p
Alias: Nup84p
Species: Saccharomyces cerevisiae S288c
Products:     NUP84

Top Publications

  1. Alber F, Dokudovskaya S, Veenhoff L, Zhang W, Kipper J, Devos D, et al. The molecular architecture of the nuclear pore complex. Nature. 2007;450:695-701 pubmed
    ..These findings provide clues to the evolutionary origins of the NPC. ..
  2. Light W, Brickner D, Brand V, Brickner J. Interaction of a DNA zip code with the nuclear pore complex promotes H2A.Z incorporation and INO1 transcriptional memory. Mol Cell. 2010;40:112-25 pubmed publisher
    ..Thus, interaction of recently repressed INO1 with the NPC alters its chromatin structure and rate of reactivation...
  3. Nagy V, Hsia K, Debler E, Kampmann M, Davenport A, Blobel G, et al. Structure of a trimeric nucleoporin complex reveals alternate oligomerization states. Proc Natl Acad Sci U S A. 2009;106:17693-8 pubmed publisher
    The heptameric Nup84 complex constitutes an evolutionarily conserved building block of the nuclear pore complex...
  4. Brohawn S, Schwartz T. Molecular architecture of the Nup84-Nup145C-Sec13 edge element in the nuclear pore complex lattice. Nat Struct Mol Biol. 2009;16:1173-7 pubmed publisher
    ..Here we present the crystal structure of the heterotrimeric 134-kDa complex of Nup84-Nup145C-Sec13 of the Y complex...
  5. Nagai S, Dubrana K, Tsai Pflugfelder M, Davidson M, Roberts T, Brown G, et al. Functional targeting of DNA damage to a nuclear pore-associated SUMO-dependent ubiquitin ligase. Science. 2008;322:597-602 pubmed publisher
    ..Relocation required the Nup84 complex and Mec1/Tel1 kinases...
  6. Kampmann M, Blobel G. Three-dimensional structure and flexibility of a membrane-coating module of the nuclear pore complex. Nat Struct Mol Biol. 2009;16:782-8 pubmed publisher
    ..We optimized the isolation of a putative membrane-coating subcomplex of the nuclear pore complex, the heptameric Nup84 complex, and analyzed its structure by EM. Our data confirmed the previously reported 'Y' shape...
  7. Santos Rosa H, Leung J, Grimsey N, Peak Chew S, Siniossoglou S. The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth. EMBO J. 2005;24:1931-41 pubmed
    ..Our data suggest that Smp2 is a critical factor in coordinating phospholipid biosynthesis at the nuclear/ER membrane with nuclear growth during the cell cycle. ..
  8. Gao H, Sumanaweera N, Bailer S, Stochaj U. Nuclear accumulation of the small GTPase Gsp1p depends on nucleoporins Nup133p, Rat2p/Nup120p, Nup85p, Nic96p, and the acetyl-CoA carboxylase Acc1p. J Biol Chem. 2003;278:25331-40 pubmed
    ..Taken together, components of the NPC subcomplex containing Rat2p/Nup120p, Nup133p, and Nup85p, in addition to proteins Nic96p and Mtr7p, are shown to be crucial for the formation of a nucleocytoplasmic Gsp1p gradient. ..
  9. Kosova B, Pante N, Rollenhagen C, Podtelejnikov A, Mann M, Aebi U, et al. Mlp2p, a component of nuclear pore attached intranuclear filaments, associates with nic96p. J Biol Chem. 2000;275:343-50 pubmed
    ..Double disruption mutants of MLP1 and MLP2 are viable and apparently not impaired in nucleocytoplasmic transport. However, overproduction of MLP1 causes nuclear accumulation of poly(A)(+) RNA in a chromatin-free area of the nucleus...

More Information

Publications44

  1. Fabre E, Hurt E. Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet. 1997;31:277-313 pubmed
    ..We review here our current knowledge on the role of nucleoporins, and on the mechanism of nucleocytoplasmic transport, with emphasis on the yeast Saccharomyces cerevisiae. ..
  2. Siniossoglou S, Wimmer C, Rieger M, Doye V, Tekotte H, Weise C, et al. A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores. Cell. 1996;84:265-75 pubmed
    In a genetic screen for nucleoporin-interacting components, a novel nuclear pore protein Nup84p, which exhibits homology to mammalian Nup107p, was isolated...
  3. Belgareh N, Rabut G, Bai S, van Overbeek M, Beaudouin J, Daigle N, et al. An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells. J Cell Biol. 2001;154:1147-60 pubmed
    ..and immunoprecipitation experiments revealed a direct and evolutionarily conserved interaction between Nup133 and Nup84/Nup107 and indicated that hNup133 and hNup107 are part of a NPC subcomplex that contains two other nucleoporins (..
  4. Siniossoglou S, Lutzmann M, Santos Rosa H, Leonard K, Mueller S, Aebi U, et al. Structure and assembly of the Nup84p complex. J Cell Biol. 2000;149:41-54 pubmed
    The Nup84p complex consists of five nucleoporins (Nup84p, Nup85p, Nup120p, Nup145p-C, and Seh1p) and Sec13p, a bona fide subunit of the COPII coat complex...
  5. Siniossoglou S, Santos Rosa H, Rappsilber J, Mann M, Hurt E. A novel complex of membrane proteins required for formation of a spherical nucleus. EMBO J. 1998;17:6449-64 pubmed
    Two membrane proteins were identified through their genetic interaction with the nucleoporin Nup84p and shown to participate in nuclear envelope morphogenesis in yeast...
  6. Palancade B, Liu X, Garcia Rubio M, Aguilera A, Zhao X, Doye V. Nucleoporins prevent DNA damage accumulation by modulating Ulp1-dependent sumoylation processes. Mol Biol Cell. 2007;18:2912-23 pubmed
    ..We previously established that the Nup84 complex, a major NPC building block, is part of a genetic network involved in DNA repair...
  7. Chadrin A, Hess B, San Roman M, Gatti X, Lombard B, Loew D, et al. Pom33, a novel transmembrane nucleoporin required for proper nuclear pore complex distribution. J Cell Biol. 2010;189:795-811 pubmed publisher
    ..Pom33 becomes critical for yeast viability in the absence of a functional Nup84 complex or Ndc1 interaction network, which are two core NPC subcomplexes, and associates with the reticulon Rtn1...
  8. Seo H, Ma Y, Debler E, Wacker D, Kutik S, Blobel G, et al. Structural and functional analysis of Nup120 suggests ring formation of the Nup84 complex. Proc Natl Acad Sci U S A. 2009;106:14281-6 pubmed publisher
    The Nup84 complex constitutes a key building block in the nuclear pore complex (NPC)...
  9. Teixeira M, Siniossoglou S, Podtelejnikov S, Benichou J, Mann M, Dujon B, et al. Two functionally distinct domains generated by in vivo cleavage of Nup145p: a novel biogenesis pathway for nucleoporins. EMBO J. 1997;16:5086-97 pubmed
    ..a carboxy-terminal domain (C-Nup145p) which is located at the nuclear pore complex (NPC) and assembles into the Nup84p complex, and a GLFG-containing amino-terminal domain (N-Nup145p) which is not part of this complex...
  10. Lutzmann M, Kunze R, Buerer A, Aebi U, Hurt E. Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins. EMBO J. 2002;21:387-97 pubmed
    ..Here, we report the reconstitution of seven proteins (Nup133p, Nup145p-C, Nup120p, Nup85p, Nup84p, Seh1p and Sec13p) into a heptameric 0.5 MDa nuclear pore subcomplex...
  11. Therizols P, Fairhead C, Cabal G, Genovesio A, Olivo Marin J, Dujon B, et al. Telomere tethering at the nuclear periphery is essential for efficient DNA double strand break repair in subtelomeric region. J Cell Biol. 2006;172:189-99 pubmed
    ..Here, using fluorescence microscopy in living cells, we show that nuclear pore proteins of the Nup84 core complex, Nup84p, Nup145Cp, Nup120p, and Nup133p, serve to anchor telomere XI-L at the nuclear periphery...
  12. Chang M, Bellaoui M, Boone C, Brown G. A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage. Proc Natl Acad Sci U S A. 2002;99:16934-9 pubmed
    ..These genes may promote replication fork stability or processivity during encounters between replication forks and DNA damage. ..
  13. Tous C, Rondon A, Garcia Rubio M, González Aguilera C, Luna R, Aguilera A. A novel assay identifies transcript elongation roles for the Nup84 complex and RNA processing factors. EMBO J. 2011;30:1953-64 pubmed publisher
    ..Next, we undertook a mutant screen and found out that the Nup84 nucleoporin complex is also required for transcription elongation, as confirmed by the GLRO assay and RNA ..
  14. Damelin M, Silver P. In situ analysis of spatial relationships between proteins of the nuclear pore complex. Biophys J. 2002;83:3626-36 pubmed
    ..We suggest that the approach may serve as a prototype for the in situ study of other large macromolecular complexes. ..
  15. Thomsen R, Saguez C, Nasser T, Jensen T. General, rapid, and transcription-dependent fragmentation of nucleolar antigens in S. cerevisiae mRNA export mutants. RNA. 2008;14:706-16 pubmed publisher
    ..Consistent with a causal role of excess nuclear mRNA, nucleolar morphology in an mRNA export mutant environment remains intact when transcription by RNA polymerase II is inhibited. ..
  16. Gulati S, Balderes D, Kim C, Guo Z, Wilcox L, Area Gomez E, et al. ATP-binding cassette transporters and sterol O-acyltransferases interact at membrane microdomains to modulate sterol uptake and esterification. FASEB J. 2015;29:4682-94 pubmed publisher
  17. Regot S, de Nadal E, Rodriguez Navarro S, Gonzalez Novo A, Pérez Fernández J, Gadal O, et al. The Hog1 stress-activated protein kinase targets nucleoporins to control mRNA export upon stress. J Biol Chem. 2013;288:17384-98 pubmed publisher
    ..Together, proper mRNA biogenesis of stress-responsive genes requires of the coordinate action of synthesis and export machineries by the Hog1 SAPK. ..
  18. Yewdell W, Colombi P, Makhnevych T, Lusk C. Lumenal interactions in nuclear pore complex assembly and stability. Mol Biol Cell. 2011;22:1375-88 pubmed publisher
    ..These findings support a role for Heh1p in the assembly or stability of the NPC, potentially through the formation of a lumenal bridge with Pom152p...
  19. Patel S, Rexach M. Discovering novel interactions at the nuclear pore complex using bead halo: a rapid method for detecting molecular interactions of high and low affinity at equilibrium. Mol Cell Proteomics. 2008;7:121-31 pubmed
    ..Halo uncovered novel interactions between the importin Kap95 and the nucleoporins (nups) Nic96, Pom34, Gle1, Ndc1, Nup84, and Seh1, which likely occur during nuclear pore complex biogenesis...
  20. Chung D, Chan J, Strecker J, Zhang W, Ebrahimi Ardebili S, Lu T, et al. Perinuclear tethers license telomeric DSBs for a broad kinesin- and NPC-dependent DNA repair process. Nat Commun. 2015;6:7742 pubmed publisher
    ..Cik1-Kar3) cooperates with chromatin remodellers to mediate interactions between subtelomeric DSBs and the Nup84 nuclear pore complex to ensure cell survival via break-induced replication (BIR), an error-prone DNA repair ..
  21. Dokudovskaya S, Waharte F, Schlessinger A, Pieper U, Devos D, Cristea I, et al. A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae. Mol Cell Proteomics. 2011;10:M110.006478 pubmed publisher
    ..These data demonstrate that the SEA complex is an additional member of a family of membrane coating and vesicle tethering assemblies, extending the repertoire of protocoatomer-related complexes. ..
  22. Grimsey N, Han G, O Hara L, Rochford J, Carman G, Siniossoglou S. Temporal and spatial regulation of the phosphatidate phosphatases lipin 1 and 2. J Biol Chem. 2008;283:29166-74 pubmed publisher
    ..We propose that distinct and non-redundant functions of lipin 1 and 2 regulate lipid production during the cell cycle and adipocyte differentiation. ..
  23. Ruben G, Kirkland J, Macdonough T, Chen M, Dubey R, Gartenberg M, et al. Nucleoporin mediated nuclear positioning and silencing of HMR. PLoS ONE. 2011;6:e21923 pubmed publisher
    ..We propose that localization of NUPs to the tDNA insulator at HMR helps maintain the intranuclear position of the silent locus, which in turn contributes to the fidelity of silencing at HMR. ..
  24. Hayakawa A, Babour A, Sengmanivong L, Dargemont C. Ubiquitylation of the nuclear pore complex controls nuclear migration during mitosis in S. cerevisiae. J Cell Biol. 2012;196:19-27 pubmed publisher
    ..This led to defects in nuclear segregation at the onset of mitosis. Thus, defining ubiquitylation of the yeast NPC highlights yet-unexplored functions of this essential organelle in cell division. ..
  25. Fernandez Martinez J, Phillips J, Sekedat M, Diaz Avalos R, Velázquez Muriel J, Franke J, et al. Structure-function mapping of a heptameric module in the nuclear pore complex. J Cell Biol. 2012;196:419-34 pubmed publisher
    ..integrative approach to determine the structure of an essential component of the yeast NPC, the ~600-kD heptameric Nup84 complex, to a precision of ~1.5 nm...
  26. Damelin M, Silver P. Mapping interactions between nuclear transport factors in living cells reveals pathways through the nuclear pore complex. Mol Cell. 2000;5:133-40 pubmed
    ..These data demonstrate the utility of FRET in mapping dynamic protein interactions in a genetic system. Furthermore, the data indicate that an importin and exportin have overlapping pathways through the NPC. ..
  27. Su X, Dion V, Gasser S, Freudenreich C. Regulation of recombination at yeast nuclear pores controls repair and triplet repeat stability. Genes Dev. 2015;29:1006-17 pubmed publisher
    ..Relocation requires Nup84 and the Slx5/8 SUMO-dependent ubiquitin ligase but not Rad51, Mec1, or Tel1...
  28. Kim S, Fernandez Martinez J, Sampathkumar P, Martel A, Matsui T, Tsuruta H, et al. Integrative structure-function mapping of the nucleoporin Nup133 suggests a conserved mechanism for membrane anchoring of the nuclear pore complex. Mol Cell Proteomics. 2014;13:2911-26 pubmed publisher
    ..Nup133, a major component in the essential Y-shaped Nup84 complex, is a large scaffold protein of the NPC's outer ring structure...
  29. Yao W, Lutzmann M, Hurt E. A versatile interaction platform on the Mex67-Mtr2 receptor creates an overlap between mRNA and ribosome export. EMBO J. 2008;27:6-16 pubmed
    ..We show that Mex67-Mtr2 through the same surface that recruits pre-60S particles interacts with the Nup84 complex, a structural module of the nuclear pore complex devoid of Phe-Gly domains...
  30. González Aguilera C, Tous C, Gómez González B, Huertas P, Luna R, Aguilera A. The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability. Mol Biol Cell. 2008;19:4310-8 pubmed publisher
  31. Sarma N, Buford T, Haley T, Barbara Haley K, Santangelo G, Willis K. The nuclear pore complex mediates binding of the Mig1 repressor to target promoters. PLoS ONE. 2011;6:e27117 pubmed publisher
    ..We propose that the NPC contributes to both repression and activation at the level of transcription. ..
  32. Casey A, Chen S, Novick P, Ferro Novick S, Wente S. Nuclear pore complex integrity requires Lnp1, a regulator of cortical endoplasmic reticulum. Mol Biol Cell. 2015;26:2833-44 pubmed publisher
    ..These findings suggest that Lnp1's role in NPC integrity is separable from functions in the ER and is linked to Ndc1 and Rtn1 interactions. ..
  33. Lokareddy R, Hapsari R, van Rheenen M, Pumroy R, Bhardwaj A, Steen A, et al. Distinctive Properties of the Nuclear Localization Signals of Inner Nuclear Membrane Proteins Heh1 and Heh2. Structure. 2015;23:1305-1316 pubmed publisher
    ..Thus, h1/h2NLSs delineate a novel class of super-potent, IBB-like membrane protein NLSs, distinct from classical NLSs found in soluble cargos and of general interest in biology. ..
  34. Niño C, Guet D, Gay A, Brutus S, Jourquin F, Mendiratta S, et al. Posttranslational marks control architectural and functional plasticity of the nuclear pore complex basket. J Cell Biol. 2016;212:167-80 pubmed publisher
    ..in regulating the association dynamics of Nup60 and its partner, Nup2, with the NPC through an interaction with Nup84, a component of the Y complex...
  35. Hung N, Lo K, Patel S, Helmke K, Johnson A. Arx1 is a nuclear export receptor for the 60S ribosomal subunit in yeast. Mol Biol Cell. 2008;19:735-44 pubmed
    ..These results show that Arx1 can directly bridge the interaction between the pre-60S particle and the NPC and thus is a third export receptor for the 60S subunit in yeast. ..