Gene Symbol: NUP53
Description: FG-nucleoporin NUP53
Alias: FG-nucleoporin NUP53
Species: Saccharomyces cerevisiae S288c
Products:     NUP53

Top Publications

  1. Marelli M, Aitchison J, Wozniak R. Specific binding of the karyopherin Kap121p to a subunit of the nuclear pore complex containing Nup53p, Nup59p, and Nup170p. J Cell Biol. 1998;143:1813-30 pubmed
    ..relevance of the interaction between Nup53p and Kap121p was further underscored by the observation that NUP53 mutations alter the subcellular distribution of Kap121p and the Kap121p- mediated import of a ribosomal L25 ..
  2. Damelin M, Silver P. Mapping interactions between nuclear transport factors in living cells reveals pathways through the nuclear pore complex. Mol Cell. 2000;5:133-40 pubmed
    ..These data demonstrate the utility of FRET in mapping dynamic protein interactions in a genetic system. Furthermore, the data indicate that an importin and exportin have overlapping pathways through the NPC. ..
  3. Chadrin A, Hess B, San Roman M, Gatti X, Lombard B, Loew D, et al. Pom33, a novel transmembrane nucleoporin required for proper nuclear pore complex distribution. J Cell Biol. 2010;189:795-811 pubmed publisher
    ..We hypothesize that, by modifying or stabilizing the nuclear envelope-NPC interface, Pom33 may contribute to proper distribution and/or efficient assembly of nuclear pores. ..
  4. Lusk C, Makhnevych T, Marelli M, Aitchison J, Wozniak R. Karyopherins in nuclear pore biogenesis: a role for Kap121p in the assembly of Nup53p into nuclear pore complexes. J Cell Biol. 2002;159:267-78 pubmed
    ..We propose that fluctuations between these two states drive the binding and release of Kap121p from Nup53p, thus facilitating Kap121p's movement through the NPC. ..
  5. Onischenko E, Stanton L, Madrid A, Kieselbach T, Weis K. Role of the Ndc1 interaction network in yeast nuclear pore complex assembly and maintenance. J Cell Biol. 2009;185:475-91 pubmed publisher
    ..with the transmembrane proteins Pom152 and Pom34 and two alternative complexes with the soluble nucleoporins Nup53 and Nup59, which in turn bind to Nup170 and Nup157...
  6. Patel S, Rexach M. Discovering novel interactions at the nuclear pore complex using bead halo: a rapid method for detecting molecular interactions of high and low affinity at equilibrium. Mol Cell Proteomics. 2008;7:121-31 pubmed
    ..The hypothesis that Nup53 and Nup59 bind directly to membranes through a C-terminal amphipathic alpha helix and to DNA via an RNA ..
  7. Lusk C, Waller D, Makhnevych T, Dienemann A, Whiteway M, Thomas D, et al. Nup53p is a target of two mitotic kinases, Cdk1p and Hrr25p. Traffic. 2007;8:647-60 pubmed
    ..The ability of nup53 alleles containing Cdk1p site mutations to complement synthetic defects of nup53 Delta nup170 Delta strains is ..
  8. Alber F, Dokudovskaya S, Veenhoff L, Zhang W, Kipper J, Devos D, et al. The molecular architecture of the nuclear pore complex. Nature. 2007;450:695-701 pubmed
    ..These findings provide clues to the evolutionary origins of the NPC. ..
  9. Fabre E, Hurt E. Yeast genetics to dissect the nuclear pore complex and nucleocytoplasmic trafficking. Annu Rev Genet. 1997;31:277-313 pubmed
    ..We review here our current knowledge on the role of nucleoporins, and on the mechanism of nucleocytoplasmic transport, with emphasis on the yeast Saccharomyces cerevisiae. ..

More Information


  1. Makhnevych T, Lusk C, Anderson A, Aitchison J, Wozniak R. Cell cycle regulated transport controlled by alterations in the nuclear pore complex. Cell. 2003;115:813-23 pubmed
    ..We propose that fluctuations in Kap121p transport mediated by the NPC contribute to controlling the subcellular distribution of molecules that direct progression through mitosis. ..
  2. Fahrenkrog B, Hubner W, Mandinova A, Pante N, Keller W, Aebi U. The yeast nucleoporin Nup53p specifically interacts with Nic96p and is directly involved in nuclear protein import. Mol Biol Cell. 2000;11:3885-96 pubmed
    ..Whereas Nup53p is directly involved in NLS-mediated protein import by its interaction with the yeast nuclear import receptor Kap95p, it appears not to participate in NES-dependent nuclear export. ..
  3. Cairo L, Wozniak R. The Nuclear Transport Factor Kap121 Is Required for Stability of the Dam1 Complex and Mitotic Kinetochore Bi-orientation. Cell Rep. 2016;14:2440-50 pubmed publisher
    ..We propose that Kap121 stabilizes the Dam1 complex and participates in escorting it to spindle MTs. ..
  4. Damelin M, Silver P. In situ analysis of spatial relationships between proteins of the nuclear pore complex. Biophys J. 2002;83:3626-36 pubmed
    ..We suggest that the approach may serve as a prototype for the in situ study of other large macromolecular complexes. ..
  5. Kobayashi J, Matsuura Y. Structural basis for cell-cycle-dependent nuclear import mediated by the karyopherin Kap121p. J Mol Biol. 2013;425:1852-1868 pubmed publisher
    ..Comparison of the NLS and RanGTP complexes reveals that RanGTP binding not only occludes the cargo-binding site but also forces Kap121p into a conformation that is incompatible with NLS recognition. ..
  6. Yewdell W, Colombi P, Makhnevych T, Lusk C. Lumenal interactions in nuclear pore complex assembly and stability. Mol Biol Cell. 2011;22:1375-88 pubmed publisher
    ..These findings support a role for Heh1p in the assembly or stability of the NPC, potentially through the formation of a lumenal bridge with Pom152p...
  7. Hayakawa A, Babour A, Sengmanivong L, Dargemont C. Ubiquitylation of the nuclear pore complex controls nuclear migration during mitosis in S. cerevisiae. J Cell Biol. 2012;196:19-27 pubmed publisher
    ..This led to defects in nuclear segregation at the onset of mitosis. Thus, defining ubiquitylation of the yeast NPC highlights yet-unexplored functions of this essential organelle in cell division. ..
  8. Tetenbaum Novatt J, Hough L, Mironska R, McKenney A, Rout M. Nucleocytoplasmic transport: a role for nonspecific competition in karyopherin-nucleoporin interactions. Mol Cell Proteomics. 2012;11:31-46 pubmed publisher
  9. Sarma N, Buford T, Haley T, Barbara Haley K, Santangelo G, Willis K. The nuclear pore complex mediates binding of the Mig1 repressor to target promoters. PLoS ONE. 2011;6:e27117 pubmed publisher
    ..We propose that the NPC contributes to both repression and activation at the level of transcription. ..
  10. Mészáros N, Cibulka J, Mendiburo M, Romanauska A, Schneider M, Köhler A. Nuclear pore basket proteins are tethered to the nuclear envelope and can regulate membrane curvature. Dev Cell. 2015;33:285-98 pubmed publisher
    ..Basket amphipathic helices are functionally linked to distinct transmembrane nucleoporins of the NPC core, suggesting a key contribution to the membrane remodeling events that underlie NPC assembly. ..
  11. Weberruss M, Savulescu A, Jando J, Bissinger T, Harel A, Glickman M, et al. Blm10 facilitates nuclear import of proteasome core particles. EMBO J. 2013;32:2697-707 pubmed publisher
    ..Thus, Blm10 represents the first CP-dedicated nuclear import receptor in yeast...
  12. Cairo L, Ptak C, Wozniak R. Mitosis-specific regulation of nuclear transport by the spindle assembly checkpoint protein Mad1p. Mol Cell. 2013;49:109-20 pubmed publisher
    ..We propose that a distinct branch of the SAC exists in which Mad1p senses unattached kinetochores and, by altering NPC transport activity, regulates the nuclear environment of the spindle. ..
  13. Makhnevych T, Ptak C, Lusk C, Aitchison J, Wozniak R. The role of karyopherins in the regulated sumoylation of septins. J Cell Biol. 2007;177:39-49 pubmed
    ..We present a model in which Ulp1p is maintained at the NPC during interphase and transiently interacts with the septin ring during mitosis. ..
  14. Iouk T, Kerscher O, Scott R, Basrai M, Wozniak R. The yeast nuclear pore complex functionally interacts with components of the spindle assembly checkpoint. J Cell Biol. 2002;159:807-19 pubmed
    ..Furthermore, we show that the association of Mad1p with the NPC is not passive and that it plays a role in nuclear transport. ..
  15. Scott R, Lusk C, Dilworth D, Aitchison J, Wozniak R. Interactions between Mad1p and the nuclear transport machinery in the yeast Saccharomyces cerevisiae. Mol Biol Cell. 2005;16:4362-74 pubmed
    ..Our further analysis also showed that only the C terminus of Mad1p is required for SAC function and that the NPC, through Nup53p, may act to regulate the duration of the SAC response. ..
  16. Patel S, Belmont B, Sante J, Rexach M. Natively unfolded nucleoporins gate protein diffusion across the nuclear pore complex. Cell. 2007;129:83-96 pubmed
    ..The results support a two-gate model of NPC architecture featuring a central diffusion gate formed by a meshwork of cohesive FG nucleoporin filaments and a peripheral gate formed by repulsive FG nucleoporin filaments...
  17. Stuwe T, Lin D, Collins L, Hurt E, Hoelz A. Evidence for an evolutionary relationship between the large adaptor nucleoporin Nup192 and karyopherins. Proc Natl Acad Sci U S A. 2014;111:2530-5 pubmed publisher
    ..Biochemically, we identify a conserved patch that binds an unstructured segment in Nup53 and show that a C-terminal tail region binds to a putative helical fragment in Nic96...