NUM1

Summary

Gene Symbol: NUM1
Description: Num1p
Alias: PAC12, Num1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Farkasovsky M, Küntzel H. Yeast Num1p associates with the mother cell cortex during S/G2 phase and affects microtubular functions. J Cell Biol. 1995;131:1003-14 pubmed
    ..of NUM1 mRNA fluctuates during the cell cycle, reaching a maximum at S/G2 phase, and the translation product Num1p associates with the cortex of mother cells mainly during S, G2, and mitosis, as seen by indirect ..
  2. Schwartz K, Richards K, Botstein D. BIM1 encodes a microtubule-binding protein in yeast. Mol Biol Cell. 1997;8:2677-91 pubmed
    ..A bim1 deletion displays synthetic lethality with deletion alleles of bik1, num1, and bub3 as well as a limited subset of tub1 conditional-lethal alleles...
  3. Heil Chapdelaine R, Oberle J, Cooper J. The cortical protein Num1p is essential for dynein-dependent interactions of microtubules with the cortex. J Cell Biol. 2000;151:1337-44 pubmed
    ..How dynein and microtubules interact with the cortex is unknown. We found that cells lacking Num1p failed to exhibit dynein-dependent microtubule sliding in the bud, resulting in defective mitotic spindle movement ..
  4. Cerveny K, Studer S, Jensen R, Sesaki H. Yeast mitochondrial division and distribution require the cortical num1 protein. Dev Cell. 2007;12:363-75 pubmed
    ..of a dominant-negative Dnm1p mutant, we uncovered an unexpected role in mitochondrial division and inheritance for Num1p, a protein previously shown to facilitate nuclear migration...
  5. Farkasovsky M, Küntzel H. Cortical Num1p interacts with the dynein intermediate chain Pac11p and cytoplasmic microtubules in budding yeast. J Cell Biol. 2001;152:251-62 pubmed
    b>Num1p, a cortical 313-kD protein, controls cytoplasmic microtubule (cMT) functions and nuclear migration through the bud neck in anaphase cells...
  6. Geiser J, Schott E, Kingsbury T, Cole N, Totis L, Bhattacharyya G, et al. Saccharomyces cerevisiae genes required in the absence of the CIN8-encoded spindle motor act in functionally diverse mitotic pathways. Mol Biol Cell. 1997;8:1035-50 pubmed
    ..These findings illustrate the complex cellular interactions exhibited by Cin8p, a member of a conserved spindle motor family. ..
  7. Hoepfner D, Schaerer F, Brachat A, Wach A, Philippsen P. Reorientation of mispositioned spindles in short astral microtubule mutant spc72Delta is dependent on spindle pole body outer plaque and Kar3 motor protein. Mol Biol Cell. 2002;13:1366-80 pubmed
    ..plaque and the astral microtubule motor function of Kar3 but not Kip2/Kip3/Dhc1, or the cortex components Kar9/Num1. Our observations suggest that Spc72 is required for astral microtubule formation at the SPB half-bridge and for ..
  8. Scarfone I, Venturetti M, Hotz M, Lengefeld J, Barral Y, Piatti S. Asymmetry of the budding yeast Tem1 GTPase at spindle poles is required for spindle positioning but not for mitotic exit. PLoS Genet. 2015;11:e1004938 pubmed publisher
    ..Thus, asymmetry of the Bub2/Bfa1/Tem1 complex is crucial to control Kar9 distribution and spindle positioning during mitosis. ..
  9. Pernice W, Vevea J, Pon L. A role for Mfb1p in region-specific anchorage of high-functioning mitochondria and lifespan in Saccharomyces cerevisiae. Nat Commun. 2016;7:10595 pubmed publisher
    ..is required for mitochondrial anchorage at that site, independent of the previously identified anchorage protein Num1p. Deletion of MFB1 results in loss of the mother-tip-localized mitochondrial population, defects in mitochondrial ..

More Information

Publications19

  1. Kusch J, Meyer A, Snyder M, Barral Y. Microtubule capture by the cleavage apparatus is required for proper spindle positioning in yeast. Genes Dev. 2002;16:1627-39 pubmed
    ..Together, our results indicate that septins and septin-dependent kinases may coordinate microtubule and actin functions in cell division. ..
  2. Lammers L, Markus S. The dynein cortical anchor Num1 activates dynein motility by relieving Pac1/LIS1-mediated inhibition. J Cell Biol. 2015;211:309-22 pubmed publisher
    ..In budding yeast, dynein is offloaded to Num1 receptors from microtubule plus ends...
  3. Hoffman Sommer M, Kucharczyk R, Piekarska I, Kozlowska E, Rytka J. Mutations in the Saccharomyces cerevisiae vacuolar fusion proteins Ccz1, Mon1 and Ypt7 cause defects in cell cycle progression in a num1Delta background. Eur J Cell Biol. 2009;88:639-52 pubmed publisher
    ..In a genome-wide analysis they have also been found to interact genetically with the nuclear-migration protein Num1. In this study we analyze these synthetic effects and we show that the mutants ccz1Delta num1Delta, mon1Delta ..
  4. Wei W, Smith N, Wu X, Kim H, Seravalli J, Khalimonchuk O, et al. YCF1-mediated cadmium resistance in yeast is dependent on copper metabolism and antioxidant enzymes. Antioxid Redox Signal. 2014;21:1475-89 pubmed publisher
  5. Paumi C, Menendez J, Arnoldo A, Engels K, Iyer K, Thaminy S, et al. Mapping protein-protein interactions for the yeast ABC transporter Ycf1p by integrated split-ubiquitin membrane yeast two-hybrid analysis. Mol Cell. 2007;26:15-25 pubmed
    ..Our study provides proof of principle that iMYTH is an ideal methodology to identify physiological interactors and regulators of ABC transporters and other yeast transmembrane proteins...
  6. Lackner L, Ping H, Graef M, Murley A, Nunnari J. Endoplasmic reticulum-associated mitochondria-cortex tether functions in the distribution and inheritance of mitochondria. Proc Natl Acad Sci U S A. 2013;110:E458-67 pubmed publisher
    ..The screen identified Num1, a cortical protein implicated in mitochondrial division and distribution that also functions in nuclear migration...
  7. Tang X, Germain B, Lee W. A novel patch assembly domain in Num1 mediates dynein anchoring at the cortex during spindle positioning. J Cell Biol. 2012;196:743-56 pubmed publisher
    ..The attachment molecule Num1 is required for dynein anchoring at the cell membrane, but how Num1 assembles into stationary cortical patches and ..
  8. Ping H, Kraft L, Chen W, Nilles A, Lackner L. Num1 anchors mitochondria to the plasma membrane via two domains with different lipid binding specificities. J Cell Biol. 2016;213:513-24 pubmed publisher
    ..The core component of MECA is the multidomain protein Num1, which assembles into clusters at the cell cortex. We show Num1 adopts an extended, polarized conformation...
  9. Klecker T, Scholz D, Förtsch J, Westermann B. The yeast cell cortical protein Num1 integrates mitochondrial dynamics into cellular architecture. J Cell Sci. 2013;126:2924-30 pubmed publisher
    ..We studied the contribution of the cell cortex protein, Num1, to mitochondrial partitioning in yeast...
  10. Chao J, Wong A, Tavassoli S, Young B, Chruscicki A, Fang N, et al. Polarization of the endoplasmic reticulum by ER-septin tethering. Cell. 2014;158:620-32 pubmed publisher
    ..ER-septin tethering polarizes the ER into separate mother and bud domains, one function of which is to position the spindle in the mother until M phase by confining the spindle capture protein Num1 to the mother ER.