Gene Symbol: NTC20
Description: Ntc20p
Alias: Ntc20p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Vincent K, Wang Q, Jay S, Hobbs K, Rymond B. Genetic interactions with CLF1 identify additional pre-mRNA splicing factors and a link between activators of yeast vesicular transport and splicing. Genetics. 2003;164:895-907 pubmed
    ..e., clf1Delta2). The identified genes encode the Mud2, Ntc20, Prp16, Prp17, Prp19, Prp22, and Syf2 splicing factors and four proteins without established contribution to ..
  2. Yeh T, Liu H, Chung C, Wu N, Liu Y, Cheng S. Splicing factor Cwc22 is required for the function of Prp2 and for the spliceosome to escape from a futile pathway. Mol Cell Biol. 2011;31:43-53 pubmed publisher
    ..Thus, Cwc22 represents a novel ATP-dependent step one factor besides Prp2 and Spp2 and has a distinct role from that of Spp2 in mediating the function of Prp2...
  3. Chen H, Tsao T, Chen C, Tsai W, Her L, Hsu M, et al. Snt309p modulates interactions of Prp19p with its associated components to stabilize the Prp19p-associated complex essential for pre-mRNA splicing. Proc Natl Acad Sci U S A. 1999;96:5406-11 pubmed
    ..A model for how Snt309p modulates such interactions is proposed. ..
  4. Tsai W, Chow Y, Chen H, Huang K, Hong R, Jan S, et al. Cef1p is a component of the Prp19p-associated complex and essential for pre-mRNA splicing. J Biol Chem. 1999;274:9455-62 pubmed
  5. Chen C, Tsai W, Chen H, Wang C, Cheng S. Identification and characterization of two novel components of the Prp19p-associated complex, Ntc30p and Ntc20p. J Biol Chem. 2001;276:488-94 pubmed
    ..We have identified two novel components of the Prp19p-associated complex, Ntc30p and Ntc20p. Like other identified components of the complex, both Ntc30p and Ntc20p are associated with the spliceosome in ..
  6. Dahan O, Kupiec M. Mutations in genes of Saccharomyces cerevisiae encoding pre-mRNA splicing factors cause cell cycle arrest through activation of the spindle checkpoint. Nucleic Acids Res. 2002;30:4361-70 pubmed
    ..We show that mutations in ISY1 cause lethality in the absence of Ntc20p, and that the double mutant isy1Delta syf2Delta shows a temperature-dependent cell cycle arrest...
  7. Tsai R, Fu R, Yeh F, Tseng C, Lin Y, Huang Y, et al. Spliceosome disassembly catalyzed by Prp43 and its associated components Ntr1 and Ntr2. Genes Dev. 2005;19:2991-3003 pubmed
    ..This is the first demonstration of physical disassembly of the spliceosome, catalyzed by a complex containing a DExD/H-box RNA helicase and two accessory factors, which might function in targeting the helicase to the correct substrate. ..
  8. Gahura O, Abrhámová K, Skruzny M, Valentová A, Munzarová V, Folk P, et al. Prp45 affects Prp22 partition in spliceosomal complexes and splicing efficiency of non-consensus substrates. J Cell Biochem. 2009;106:139-51 pubmed publisher
    ..lethality screen, we found that prp45(1-169) genetically interacts with alleles of NTC members SYF1, CLF1/SYF3, NTC20, and CEF1, and 2nd step splicing factors SLU7, PRP17, PRP18, and PRP22...
  9. Chiu Y, Liu Y, Chiang T, Yeh T, Tseng C, Wu N, et al. Cwc25 is a novel splicing factor required after Prp2 and Yju2 to facilitate the first catalytic reaction. Mol Cell Biol. 2009;29:5671-8 pubmed publisher
    ..These results have implications for the possible roles of Cwc25 and HP-X in facilitating juxtaposition of the 5' splice site and the branch point during the first catalytic reaction...

More Information


  1. Ben Yehuda S, Dix I, Russell C, McGarvey M, Beggs J, Kupiec M. Genetic and physical interactions between factors involved in both cell cycle progression and pre-mRNA splicing in Saccharomyces cerevisiae. Genetics. 2000;156:1503-17 pubmed
    ..and biochemical analysis, we show that the SYF gene products interact with each other and with four other proteins: Isy1p, Cef1p, Prp22p, and Ntc20p. We discuss the role played by these proteins in splicing and cell cycle progression.
  2. Chen C, Yu W, Tsao T, Wang L, Chen H, Lin J, et al. Functional and physical interactions between components of the Prp19p-associated complex. Nucleic Acids Res. 2002;30:1029-37 pubmed
    ..and biochemical methods we previously identified four components of this complex, Ntc25p, Ntc85p, Ntc30p and Ntc20p, all of them being novel splicing factors...
  3. Liu Y, Chen H, Wu N, Cheng S. A novel splicing factor, Yju2, is associated with NTC and acts after Prp2 in promoting the first catalytic reaction of pre-mRNA splicing. Mol Cell Biol. 2007;27:5403-13 pubmed
  4. Chang K, Chen H, Cheng S. Ntc90 is required for recruiting first step factor Yju2 but not for spliceosome activation. RNA. 2009;15:1729-39 pubmed publisher
    ..It forms a stable complex with Ntc31, Ntc30, and Ntc20 in the absence of Ntc25, when other interactions between NTC components are disrupted...
  5. Villa T, Guthrie C. The Isy1p component of the NineTeen complex interacts with the ATPase Prp16p to regulate the fidelity of pre-mRNA splicing. Genes Dev. 2005;19:1894-904 pubmed
    ..We propose that deletion of ISY1 favors the premature release of Prp16p, thus promoting second-step chemistry of precursors with inappropriate 3'-splice sites. ..