Gene Symbol: NSE5
Description: Smc5-Smc6 complex subunit NSE5
Alias: Smc5-Smc6 complex subunit NSE5
Species: Saccharomyces cerevisiae S288c
Zhao X, Blobel G. A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization. Proc Natl Acad Sci U S A. 2005;102:4777-82 pubmed
..We propose that Mms21 sumoylates proteins involved in these diverse processes and that the other members of the complex, particularly Smc5/6, facilitate proper substrate sumoylation by localizing Mms21 to specific chromosomal regions. ..
Bustard D, Menolfi D, Jeppsson K, Ball L, Dewey S, Shirahige K, et al
. During replication stress, non-SMC element 5 (NSE5) is required for Smc5/6 protein complex functionality at stalled forks. J Biol Chem. 2012;287:11374-83 pubmed publisher
..Characterization of two temperature-sensitive mutants, nse5-ts1 and nse5-ts2, demonstrated the importance of Nse5 within the Smc5/6 complex for its stability and functionality ..
Duan X, Yang Y, Chen Y, Arenz J, Rangi G, Zhao X, et al
. Architecture of the Smc5/6 Complex of Saccharomyces cerevisiae Reveals a Unique Interaction between the Nse5-6 Subcomplex and the Hinge Regions of Smc5 and Smc6. J Biol Chem. 2009;284:8507-15 pubmed publisher
..Nse2 binds to the middle of the coiled-coil region of Smc5. Nse5 and Nse6 interact with each other and, as a heterodimer, bind to the hinge regions of Smc5 and Smc6...
BermÃºdez LÃ³pez M, PociÃ±o Merino I, SÃ¡nchez H, Bueno A, Guasch C, Almedawar S, et al
. ATPase-dependent control of the Mms21 SUMO ligase during DNA repair. PLoS Biol. 2015;13:e1002089 pubmed publisher
Kanno T, Berta D, SjÃ¶gren C. The Smc5/6 Complex Is an ATP-Dependent Intermolecular DNA Linker. Cell Rep. 2015;12:1471-82 pubmed publisher
..Taken together, these results indicate that Smc5/6 executes its cellular functions through ATP-regulated intermolecular DNA linking. ..
Horigome C, Bustard D, Marcomini I, Delgoshaie N, Tsai Pflugfelder M, Cobb J, et al
. PolySUMOylation by Siz2 and Mms21 triggers relocation of DNA breaks to nuclear pores through the Slx5/Slx8 STUbL. Genes Dev. 2016;30:931-45 pubmed publisher
..Slx5/Slx8 and binding to pores favor repair by ectopic break-induced replication and imprecise end-joining. ..
Bustard D, Ball L, Cobb J. Non-Smc element 5 (Nse5) of the Smc5/6 complex interacts with SUMO pathway components. Biol Open. 2016;5:777-85 pubmed publisher
..Our characterization of Nse5 establishes a previously unidentified relationship between the Smc5/6 complex and factors of the SUMO pathway...
Ohouo P, Bastos de Oliveira F, Almeida B, Smolka M. DNA damage signaling recruits the Rtt107-Slx4 scaffolds via Dpb11 to mediate replication stress response. Mol Cell. 2010;39:300-6 pubmed publisher
..Our results unveil how Mec1 regulates the Slx4 and Rtt107 scaffolds and establish a mechanistic link between DNA damage signaling and fork repair. ..
Leung G, Lee L, Schmidt T, Shirahige K, Kobor M. Rtt107 is required for recruitment of the SMC5/6 complex to DNA double strand breaks. J Biol Chem. 2011;286:26250-7 pubmed publisher
..Genetic analyses revealed that, although a subset of Rtt107 and SMC5/6 functions was shared, these proteins also contributed independently to maintenance of genome integrity. ..