Gene Symbol: NSA2
Description: rRNA-processing protein NSA2
Alias: rRNA-processing protein NSA2
Species: Saccharomyces cerevisiae S288c
Harnpicharnchai P, Jakovljevic J, Horsey E, Miles T, Roman J, Rout M, et al
. Composition and functional characterization of yeast 66S ribosome assembly intermediates. Mol Cell. 2001;8:505-15 pubmed
..These results demonstrate that proteomic approaches in concert with genetic tools provide powerful means to purify and characterize ribosome assembly intermediates. ..
Lebreton A, Saveanu C, Decourty L, Jacquier A, Fromont Racine M. Nsa2 is an unstable, conserved factor required for the maturation of 27 SB pre-rRNAs. J Biol Chem. 2006;281:27099-108 pubmed
..The essential pre-ribosomal 30-kDa protein, Nsa2, was characterized as one of the most conserved proteins from yeast to human...
Dembowski J, Ramesh M, McManus C, Woolford J. Identification of the binding site of Rlp7 on assembling 60S ribosomal subunits in Saccharomyces cerevisiae. RNA. 2013;19:1639-47 pubmed publisher
..Thus, we propose that Rlp7 coevolved with these RNA elements to facilitate eukaryotic-specific functions in ribosome assembly and pre-rRNA processing. ..
McCann K, Teramoto T, Zhang J, Tanaka Hall T, Baserga S. The molecular basis for ANE syndrome revealed by the large ribosomal subunit processome interactome. elife. 2016;5: pubmed publisher
Occhipinti L, Chang Y, Altvater M, Menet A, Kemmler S, Panse V. Non-FG mediated transport of the large pre-ribosomal subunit through the nuclear pore complex by the mRNA export factor Gle2. Nucleic Acids Res. 2013;41:8266-79 pubmed publisher
..We propose that large complex cargos rely on non-FG as well as FG-interactions for their efficient translocation through the nuclear pore complex channel. ..
Fuentes J, Datta K, Sullivan S, Walker A, Maddock J. In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1. Mol Genet Genomics. 2007;278:105-23 pubmed
..Several conserved charge-to-alanine substitutions outside the GTPase domain display modest growth phenotypes indicating that these residues are not critical for function. ..
Matsuo Y, Granneman S, Thoms M, Manikas R, Tollervey D, Hurt E. Coupled GTPase and remodelling ATPase activities form a checkpoint for ribosome export. Nature. 2014;505:112-116 pubmed publisher
..We conclude that Nug2 is a regulatory GTPase that monitors pre-60S maturation, with release from its placeholder site linked to recruitment of the nuclear export machinery. ..
Talkish J, Zhang J, Jakovljevic J, Horsey E, Woolford J. Hierarchical recruitment into nascent ribosomes of assembly factors required for 27SB pre-rRNA processing in Saccharomyces cerevisiae. Nucleic Acids Res. 2012;40:8646-61 pubmed
..Inhibition of TOR kinase decreases association of Rpf2, Spb4, Nog1 and Nog2 with pre-ribosomes. ..