NPL3

Summary

Gene Symbol: NPL3
Description: mRNA-binding protein NPL3
Alias: MTR13, MTS1, NAB1, NOP3, mRNA-binding protein NPL3
Species: Saccharomyces cerevisiae S288c
Products:     NPL3

Top Publications

  1. Deka P, Bucheli M, Moore C, Buratowski S, Varani G. Structure of the yeast SR protein Npl3 and Interaction with mRNA 3'-end processing signals. J Mol Biol. 2008;375:136-50 pubmed
    Yeast Npl3 is homologous to SR proteins in higher eukaryotes, a family of RNA-binding proteins that have multiple essential roles in RNA metabolism...
  2. Lei E, Krebber H, Silver P. Messenger RNAs are recruited for nuclear export during transcription. Genes Dev. 2001;15:1771-82 pubmed
    ..Combined mutations in the Saccharomyces cerevisiae hnRNP Npl3 and TATA-binding protein (TBP) block mRNA export, implying that cotranscriptional recruitment of Npl3 is required ..
  3. Windgassen M, Sturm D, Cajigas I, Gonzalez C, Seedorf M, Bastians H, et al. Yeast shuttling SR proteins Npl3p, Gbp2p, and Hrb1p are part of the translating mRNPs, and Npl3p can function as a translational repressor. Mol Cell Biol. 2004;24:10479-91 pubmed
    ..Consistent with this idea, a mutation in NPL3 that slows down translation suppresses growth defects caused by the presence of translation inhibitors or a ..
  4. Gilbert W, Siebel C, Guthrie C. Phosphorylation by Sky1p promotes Npl3p shuttling and mRNA dissociation. RNA. 2001;7:302-13 pubmed
    ..b>Npl3 is a shuttling protein in budding yeast that we showed previously to be a substrate for the mammalian SR protein ..
  5. McBride A, Cook J, Stemmler E, Rutledge K, McGrath K, Rubens J. Arginine methylation of yeast mRNA-binding protein Npl3 directly affects its function, nuclear export, and intranuclear protein interactions. J Biol Chem. 2005;280:30888-98 pubmed
    ..We have used the yeast mRNA-binding protein Npl3 to address these questions in vivo...
  6. Siebel C, Feng L, Guthrie C, Fu X. Conservation in budding yeast of a kinase specific for SR splicing factors. Proc Natl Acad Sci U S A. 1999;96:5440-5 pubmed
    ..The unexpected discovery of an SR protein kinase in budding yeast provides a foundation for genetic dissection of the biological functions of SR proteins and their kinases. ..
  7. Xu C, Henry P, Setya A, Henry M. In vivo analysis of nucleolar proteins modified by the yeast arginine methyltransferase Hmt1/Rmt1p. RNA. 2003;9:746-59 pubmed
    ..The observation that three nucleolar proteins are modified by Hmt1p but are not exported from the nucleolus implies an alternate role for arginine methylation. ..
  8. Bucheli M, He X, Kaplan C, Moore C, Buratowski S. Polyadenylation site choice in yeast is affected by competition between Npl3 and polyadenylation factor CFI. RNA. 2007;13:1756-64 pubmed
    ..Previously, we found that the yeast hnRNP protein Npl3 can negatively regulate 3' end mRNA formation and termination at the GAL1 gene...
  9. DERMODY J, Dreyfuss J, Villen J, Ogundipe B, Gygi S, Park P, et al. Unphosphorylated SR-like protein Npl3 stimulates RNA polymerase II elongation. PLoS ONE. 2008;3:e3273 pubmed publisher
    ..The S. cerevisiae SR-like protein Npl3 functions to negatively regulate transcription termination by antagonizing the binding of polyA/termination ..

More Information

Publications68

  1. Bucheli M, Buratowski S. Npl3 is an antagonist of mRNA 3' end formation by RNA polymerase II. EMBO J. 2005;24:2150-60 pubmed
    ..Interestingly, mutations in the mRNA-binding protein Npl3 improve transcription termination...
  2. McBride A, Weiss V, Kim H, Hogle J, Silver P. Analysis of the yeast arginine methyltransferase Hmt1p/Rmt1p and its in vivo function. Cofactor binding and substrate interactions. J Biol Chem. 2000;275:3128-36 pubmed
    ..A cold-sensitive mutant of Hmt1p was generated and showed reduced methylation of Npl3p, but not of other substrates, at 14 degrees C. These results define new aspects of Hmt1 and reveal the importance of its activity in vivo. ..
  3. Wilson S, Datar K, Paddy M, Swedlow J, Swanson M. Characterization of nuclear polyadenylated RNA-binding proteins in Saccharomyces cerevisiae. J Cell Biol. 1994;127:1173-84 pubmed
    ..These results suggest that the Nab proteins may be required for packaging pre-mRNAs into ribonucleoprotein structures amenable to efficient nuclear RNA processing. ..
  4. Wong C, Qiu H, Hu C, Dong J, Hinnebusch A. Yeast cap binding complex impedes recruitment of cleavage factor IA to weak termination sites. Mol Cell Biol. 2007;27:6520-31 pubmed
    ..Importantly, deletion of CBC subunits or NPL3 also increases termination at a naturally occurring weak poly(A) site in the RNA14 coding sequences...
  5. Lukasiewicz R, Nolen B, Adams J, Ghosh G. The RGG domain of Npl3p recruits Sky1p through docking interactions. J Mol Biol. 2007;367:249-61 pubmed
    ..We suggest that docking interaction between Sky1p and Npl3p is essential for substrate recruitment and binding specificity. ..
  6. Kress T, Krogan N, Guthrie C. A single SR-like protein, Npl3, promotes pre-mRNA splicing in budding yeast. Mol Cell. 2008;32:727-34 pubmed publisher
    ..While budding yeast lack alternative splicing, they do have three SR-like proteins: Npl3, Gbp2, and Hrb1...
  7. Shen E, Henry M, Weiss V, Valentini S, Silver P, Lee M. Arginine methylation facilitates the nuclear export of hnRNP proteins. Genes Dev. 1998;12:679-91 pubmed
    ..Together, these findings establish that one biological role for arginine methylation is in facilitating the export of certain hnRNPs out of the nucleus. ..
  8. Senger B, Simos G, Bischoff F, Podtelejnikov A, Mann M, Hurt E. Mtr10p functions as a nuclear import receptor for the mRNA-binding protein Npl3p. EMBO J. 1998;17:2196-207 pubmed
    ..This suggests that Npl3p follows a distinct nuclear import pathway and that intranuclear release from its specific import receptor Mtr10p requires the cooperative action of both Ran-GTP and newly synthesized mRNA. ..
  9. Weiss V, McBride A, Soriano M, Filman D, Silver P, Hogle J. The structure and oligomerization of the yeast arginine methyltransferase, Hmt1. Nat Struct Biol. 2000;7:1165-71 pubmed
    ..Mutation of dimer contact sites eliminates activity of Hmt1 both in vivo and in vitro. Mutating residues in the acidic cavity significantly reduces binding and methylation of the substrate Npl3.
  10. Yun C, Fu X. Conserved SR protein kinase functions in nuclear import and its action is counteracted by arginine methylation in Saccharomyces cerevisiae. J Cell Biol. 2000;150:707-18 pubmed
  11. Lund M, Kress T, Guthrie C. Autoregulation of Npl3, a yeast SR protein, requires a novel downstream region and serine phosphorylation. Mol Cell Biol. 2008;28:3873-81 pubmed publisher
    b>Npl3 is an SR-like protein with documented roles in mRNA export and transcription termination. Maintaining appropriate levels of Npl3 protein is critical for cell survival...
  12. Pemberton L, Rosenblum J, Blobel G. A distinct and parallel pathway for the nuclear import of an mRNA-binding protein. J Cell Biol. 1997;139:1645-53 pubmed
    ..Thus, at least two parallel pathways function in the import of mRNA-binding proteins, suggesting the need for the coordination of these pathways. ..
  13. Gilbert W, Guthrie C. The Glc7p nuclear phosphatase promotes mRNA export by facilitating association of Mex67p with mRNA. Mol Cell. 2004;13:201-12 pubmed
    ..We propose a model whereby a cycle of cytoplasmic phosphorylation and nuclear dephosphorylation of shuttling SR adaptor proteins regulates Mex67p:Mtr2p/NXF1:p15-dependent mRNA export. ..
  14. Krebber H, Taura T, Lee M, Silver P. Uncoupling of the hnRNP Npl3p from mRNAs during the stress-induced block in mRNA export. Genes Dev. 1999;13:1994-2004 pubmed
    ..However, under conditions of stress, Npl3p no longer associates with the export complex, rendering it export incompetent and thus nuclear. ..
  15. Lee M, Henry M, Silver P. A protein that shuttles between the nucleus and the cytoplasm is an important mediator of RNA export. Genes Dev. 1996;10:1233-46 pubmed
    ..b>NPL3 encodes an RNA-binding protein that shuttles in and out of the nucleus...
  16. Xu C, Henry M. Nuclear export of hnRNP Hrp1p and nuclear export of hnRNP Npl3p are linked and influenced by the methylation state of Npl3p. Mol Cell Biol. 2004;24:10742-56 pubmed
    ..In contrast, we found that Npl3 arginine methylation not only facilitates its own export but also is required for Hrp1p to efficiently exit the ..
  17. Shen E, Stage Zimmermann T, Chui P, Silver P. 7The yeast mRNA-binding protein Npl3p interacts with the cap-binding complex. J Biol Chem. 2000;275:23718-24 pubmed
    ..In this report, we define a genetic relationship between NPL3 and the nonessential genes encoding the subunits of the cap-binding complex (CBP80 and CBP20)...
  18. Inoue K, Mizuno T, Wada K, Hagiwara M. Novel RING finger proteins, Air1p and Air2p, interact with Hmt1p and inhibit the arginine methylation of Npl3p. J Biol Chem. 2000;275:32793-9 pubmed
    ..Thus, Air1p and Air2p may affect mRNA transport by regulating the arginine methylation state of heterogeneous nuclear ribonucleoproteins...
  19. Hackmann A, Gross T, Baierlein C, Krebber H. The mRNA export factor Npl3 mediates the nuclear export of large ribosomal subunits. EMBO Rep. 2011;12:1024-31 pubmed publisher
    ..Messenger RNAs are exported through the RNA-binding protein Npl3 and the interacting export receptor Mex67...
  20. Henry M, Borland C, Bossie M, Silver P. Potential RNA binding proteins in Saccharomyces cerevisiae identified as suppressors of temperature-sensitive mutations in NPL3. Genetics. 1996;142:103-15 pubmed
    The NPL3 gene of the yeast Saccharomyces cerevisiae encodes a protein with similarity to heterogeneous nuclear ribonucleoproteins (hnRNPs)...
  21. Henry M, Silver P. A novel methyltransferase (Hmt1p) modifies poly(A)+-RNA-binding proteins. Mol Cell Biol. 1996;16:3668-78 pubmed
    ..We have previously shown that npl3-1 mutants are temperature sensitive for growth and defective for export of mRNA from the nucleus...
  22. Green D, Johnson C, Hagan H, Corbett A. The C-terminal domain of myosin-like protein 1 (Mlp1p) is a docking site for heterogeneous nuclear ribonucleoproteins that are required for mRNA export. Proc Natl Acad Sci U S A. 2003;100:1010-5 pubmed
    ..This study identifies Nab2p as a heterogeneous nuclear ribonucleoprotein found in complex with Mlp1p and begins to delineate the path that mRNA travels from the chromatin to the nuclear pore. ..
  23. Tsvetanova N, Klass D, Salzman J, Brown P. Proteome-wide search reveals unexpected RNA-binding proteins in Saccharomyces cerevisiae. PLoS ONE. 2010;5: pubmed publisher
    ..Our results suggest that many more RBPs still remain to be identified and provide a set of candidates for further investigation. ..
  24. Gomar Alba M, del Olmo M. Hot1 factor recruits co-activator Sub1 and elongation complex Spt4/5 to osmostress genes. Biochem J. 2016;473:3065-79 pubmed publisher
    ..Instead, other data presented herein indicate a key function of the Hot1 transcription factor in the recruitment of these proteins to the promoter or the 5'-coding region of the genes under its control. ..
  25. Wahba L, Amon J, Koshland D, Vuica Ross M. RNase H and multiple RNA biogenesis factors cooperate to prevent RNA:DNA hybrids from generating genome instability. Mol Cell. 2011;44:978-88 pubmed publisher
    ..In summary, RNA:DNA hybrids are a potent source for changing genome structure. By preventing their formation and accumulation, multiple RNA biogenesis factors and RNase H act as guardians of the genome. ..
  26. Skrisovska L, Allain F. Improved segmental isotope labeling methods for the NMR study of multidomain or large proteins: application to the RRMs of Npl3p and hnRNP L. J Mol Biol. 2008;375:151-64 pubmed
    ..Subsequently, the structures of the two RRMs of Npl3p were determined on the basis of samples in which each RRM was expressed individually. The two Npl3p RRMs adopt the expected beta alpha beta beta alpha beta fold. ..
  27. Chen Y, Milliman E, Goulet I, Cote J, Jackson C, Vollbracht J, et al. Protein arginine methylation facilitates cotranscriptional recruitment of pre-mRNA splicing factors. Mol Cell Biol. 2010;30:5245-56 pubmed publisher
    ..in the absence of either Hmt1 or of its catalytic activity, an association between Snp1 and the SR-like protein Npl3 is substantially increased...
  28. Gottschalk A, Tang J, Puig O, Salgado J, Neubauer G, Colot H, et al. A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins. RNA. 1998;4:374-93 pubmed
    ..Finally, we show that Nam8p/Mud15p contributes to the stability of U1 snRNP. ..
  29. Hackmann A, Wu H, Schneider U, Meyer K, Jung K, Krebber H. Quality control of spliced mRNAs requires the shuttling SR proteins Gbp2 and Hrb1. Nat Commun. 2014;5:3123 pubmed publisher
    ..Altogether, these data identify a role for shuttling SR proteins in mRNA surveillance and nuclear mRNA quality control. ..
  30. Meinel D, Burkert Kautzsch C, Kieser A, O Duibhir E, Siebert M, Mayer A, et al. Recruitment of TREX to the transcription machinery by its direct binding to the phospho-CTD of RNA polymerase II. PLoS Genet. 2013;9:e1003914 pubmed publisher
    ..In summary, we provide insight into how the phospho-code of the CTD directs mRNP formation and export through TREX recruitment...
  31. Aubol B, Ungs L, Lukasiewicz R, Ghosh G, Adams J. Chemical clamping allows for efficient phosphorylation of the RNA carrier protein Npl3. J Biol Chem. 2004;279:30182-8 pubmed
    ..both high substrate affinities and high turnover rates, the phosphorylation of the yeast RNA transport protein Npl3 by its natural protein kinase, Sky1p, was evaluated...
  32. LEE SOETY J, Jones J, Macgibeny M, Remaly E, Daniels L, Ito A, et al. Yeast hnRNP-related proteins contribute to the maintenance of telomeres. Biochem Biophys Res Commun. 2012;426:12-7 pubmed publisher
    ..The Saccharomyces cerevisiae protein Npl3 shares significant amino acid sequence similarities with hnRNP A1...
  33. Zander G, Hackmann A, Bender L, Becker D, Lingner T, Salinas G, et al. mRNA quality control is bypassed for immediate export of stress-responsive transcripts. Nature. 2016;: pubmed publisher
    ..In Saccharomyces cerevisiae, the shuttling RNA adaptor proteins Npl3, Gbp2, Hrb1 and Nab2 are loaded co-transcriptionally onto growing pre-mRNAs...
  34. Rollenhagen C, Hodge C, Cole C. Following temperature stress, export of heat shock mRNA occurs efficiently in cells with mutations in genes normally important for mRNA export. Eukaryot Cell. 2007;6:505-13 pubmed
    ..Heat shock mRNA was exported efficiently in temperature-sensitive npl3, yra1, and npl3 yra1 mutant strains. Nevertheless, Yra1p was recruited to heat shock mRNA, as were Nab2p and Npl3p...
  35. Lotan R, Bar On V, Harel Sharvit L, Duek L, Melamed D, Choder M. The RNA polymerase II subunit Rpb4p mediates decay of a specific class of mRNAs. Genes Dev. 2005;19:3004-16 pubmed
    ..In this way, Rpb4p might link the activity of the basal transcription apparatus with that of the mRNA decay machinery. ..
  36. Gemayel R, Chavali S, Pougach K, Legendre M, Zhu B, Boeynaems S, et al. Variable Glutamine-Rich Repeats Modulate Transcription Factor Activity. Mol Cell. 2015;59:615-27 pubmed publisher
    ..Thus, Q-rich repeats are dynamic functional domains that modulate a regulator's innate function, with the inherent risk of pathogenic repeat expansions. ..
  37. Huang H, Hopper A. In vivo biochemical analyses reveal distinct roles of β-importins and eEF1A in tRNA subcellular traffic. Genes Dev. 2015;29:772-83 pubmed publisher
    ..Assembly and/or stability of this quaternary complex requires Tef1/2, thereby facilitating efficient re-export of aminoacylated tRNAs to the cytoplasm. ..
  38. Balzer R, Henry M. Snu56p is required for Mer1p-activated meiotic splicing. Mol Cell Biol. 2008;28:2497-508 pubmed publisher
    ..Interestingly, these two proteins do not interact, suggesting that Snu56p links pre-mRNA-bound Mer1p to Nam8p in the U1 snRNP. This work demonstrates that the Snu56 protein is required for splicing only during meiosis. ..
  39. Muddukrishna B, Jackson C, Yu M. Protein arginine methylation of Npl3 promotes splicing of the SUS1 intron harboring non-consensus 5' splice site and branch site. Biochim Biophys Acta Gene Regul Mech. 2017;1860:730-739 pubmed publisher
    ..Here we provide evidence that protein arginine methylation of the yeast SR-/hnRNP-like protein Npl3 plays a role in facilitating efficient splicing of the SUS1 intron that harbors a non-consensus 5' splice site and ..
  40. Baierlein C, Hackmann A, Gross T, Henker L, Hinz F, Krebber H. Monosome formation during translation initiation requires the serine/arginine-rich protein Npl3. Mol Cell Biol. 2013;33:4811-23 pubmed publisher
    The yeast shuttling serine/arginine-rich protein Npl3 is required for the export of mRNAs and pre-60S ribosomal subunits from the nucleus to the cytoplasm. Here, we report a novel function of Npl3 in translation initiation...
  41. Erce M, Abeygunawardena D, Low J, Hart Smith G, Wilkins M. Interactions affected by arginine methylation in the yeast protein-protein interaction network. Mol Cell Proteomics. 2013;12:3184-98 pubmed publisher
    ..Interactions between the yeast hub protein Npl3 and yeast proteins Air2, Ded1, Gbp2, Snp1, and Yra1 were first validated in the absence of methylation...
  42. Erce M, Low J, Hart Smith G, Wilkins M. A conditional two-hybrid (C2H) system for the detection of protein-protein interactions that are mediated by post-translational modification. Proteomics. 2013;13:1059-64 pubmed publisher
    ..We demonstrate the use of the C2H system to study the dimerization of the yeast protein Npl3, which is increased when methylated by the methyltransferase Hmt1.
  43. Rajyaguru P, She M, Parker R. Scd6 targets eIF4G to repress translation: RGG motif proteins as a class of eIF4G-binding proteins. Mol Cell. 2012;45:244-54 pubmed publisher
    ..other RGG domain-containing proteins in yeast copurify with eIF4E/G and we demonstrate that two such proteins, Npl3 and Sbp1, also directly bind eIF4G and repress translation in a manner dependent on their RGG motifs...
  44. Hsieh C, Huang S, Wu Y, Liu L, Han C, Liu Y, et al. Expression of proteins with dimethylarginines in Escherichia coli for protein-protein interaction studies. Protein Sci. 2007;16:919-28 pubmed
    ..Sbp1p and Sbp1p/hmt1 were covalently attached to solid supports for the isolation of interacting proteins. The results indicate that arginine methylation on Sbp1p exerts both positive and negative effects on protein-protein interaction. ..
  45. Skruzny M, Schneider C, Rácz A, Weng J, Tollervey D, Hurt E. An endoribonuclease functionally linked to perinuclear mRNP quality control associates with the nuclear pore complexes. PLoS Biol. 2009;7:e8 pubmed publisher
    ..The data suggest that Swt1 endoribonuclease might be transiently recruited to NPCs to initiate the degradation of defective pre-mRNPs or mRNPs trapped at nuclear periphery in order to avoid their cytoplasmic export and translation...
  46. Burkard K, Butler J. A nuclear 3'-5' exonuclease involved in mRNA degradation interacts with Poly(A) polymerase and the hnRNA protein Npl3p. Mol Cell Biol. 2000;20:604-16 pubmed
    ..These findings suggest that Rrp6p may interact with the mRNA polyadenylation system and thereby play a role in a nuclear pathway for the degradation of aberrantly processed precursor mRNAs. ..
  47. Laxman S, Tu B. Multiple TORC1-associated proteins regulate nitrogen starvation-dependent cellular differentiation in Saccharomyces cerevisiae. PLoS ONE. 2011;6:e26081 pubmed publisher
    ..Our studies also suggest the CEN.PK strain background of S. cerevisiae may be particularly useful for investigations of nitrogen starvation-induced diploid pseudohyphal growth. ..
  48. Estrella L, Wilkinson M, Gonzalez C. The shuttling protein Npl3 promotes translation termination accuracy in Saccharomyces cerevisiae. J Mol Biol. 2009;394:410-22 pubmed publisher
    ..Yeast harboring the npl3-95 mutant allele have an impaired ability to translate lacZ, enhanced sensitivity to cycloheximide and paromomycin, ..
  49. Hart Smith G, Low J, Erce M, Wilkins M. Enhanced methylarginine characterization by post-translational modification-specific targeted data acquisition and electron-transfer dissociation mass spectrometry. J Am Soc Mass Spectrom. 2012;23:1376-89 pubmed publisher
    ..Using trypsin digested Saccharomyces cerevisiae Npl3, in which evidence is presented for 18 methylarginine sites-17 of which fall within a glycine-arginine-rich (GAR) ..
  50. Sobti M, Cubeddu L, Haynes P, Mabbutt B. Engineered rings of mixed yeast Lsm proteins show differential interactions with translation factors and U-rich RNA. Biochemistry. 2010;49:2335-45 pubmed publisher
    ..Our findings suggest Lsm1 and/or Lsm4 can interact with translationally active mRNA. ..
  51. Yagoub D, Hart Smith G, Moecking J, Erce M, Wilkins M. Yeast proteins Gar1p, Nop1p, Npl3p, Nsr1p, and Rps2p are natively methylated and are substrates of the arginine methyltransferase Hmt1p. Proteomics. 2015;15:3209-18 pubmed publisher
    ..This brings the total of Hmt1 substrate proteins for which native methylation sites have been identified to five. ..
  52. Savchenko A, Krogan N, Cort J, Evdokimova E, Lew J, Yee A, et al. The Shwachman-Bodian-Diamond syndrome protein family is involved in RNA metabolism. J Biol Chem. 2005;280:19213-20 pubmed publisher
    ..revealed genetic interactions with proteins involved in RNA and rRNA processing including Mdm20/Nat3, Nsr1, and Npl3. Our observations, taken together with previous reports, support the conclusion that SBDS and its homologues play a ..
  53. Occhipinti L, Chang Y, Altvater M, Menet A, Kemmler S, Panse V. Non-FG mediated transport of the large pre-ribosomal subunit through the nuclear pore complex by the mRNA export factor Gle2. Nucleic Acids Res. 2013;41:8266-79 pubmed publisher
    ..We propose that large complex cargos rely on non-FG as well as FG-interactions for their efficient translocation through the nuclear pore complex channel. ..
  54. Wong C, Tang H, Kong K, Wong G, Qiu H, Jin D, et al. Yeast arginine methyltransferase Hmt1p regulates transcription elongation and termination by methylating Npl3p. Nucleic Acids Res. 2010;38:2217-28 pubmed publisher
    ..As Hmt1p stimulates dissociation of Tho2p from an Npl3p-mRNP complex, it could act to recycle these elongation and antitermination factors back to sites of ongoing transcription. ..
  55. Santos Pereira J, Herrero A, García Rubio M, Marin A, Moreno S, Aguilera A. The Npl3 hnRNP prevents R-loop-mediated transcription-replication conflicts and genome instability. Genes Dev. 2013;27:2445-58 pubmed publisher
    ..Here we show that yeast Npl3, the most abundant RNA-binding hnRNP, prevents R-loop-mediated genome instability...
  56. Holmes R, Tuck A, Zhu C, Dunn Davies H, Kudla G, Clauder Münster S, et al. Loss of the Yeast SR Protein Npl3 Alters Gene Expression Due to Transcription Readthrough. PLoS Genet. 2015;11:e1005735 pubmed publisher
    Yeast Npl3 is a highly abundant, nuclear-cytoplasmic shuttling, RNA-binding protein, related to metazoan SR proteins. Reported functions of Npl3 include transcription elongation, splicing and RNA 3' end processing...
  57. Vitaliano Prunier A, Babour A, Hérissant L, Apponi L, Margaritis T, Holstege F, et al. H2B ubiquitylation controls the formation of export-competent mRNP. Mol Cell. 2012;45:132-9 pubmed publisher
    ..Modification of H2B thus participates in the crosstalk between cotranscriptional events and assembly of mRNPs linking nuclear processing and mRNA export. ..
  58. Lukasiewicz R, Velazquez Dones A, Huynh N, Hagopian J, Fu X, Adams J, et al. Structurally unique yeast and mammalian serine-arginine protein kinases catalyze evolutionarily conserved phosphorylation reactions. J Biol Chem. 2007;282:23036-43 pubmed
    ..Thus, there are physical attributes of SR and SR-like substrates that dictate the mechanism of phosphorylation, whereas the ability to processively phosphorylate substrates is inherent to SR protein kinases. ..
  59. Colombo C, Trovesi C, Menin L, Longhese M, Clerici M. The RNA binding protein Npl3 promotes resection of DNA double-strand breaks by regulating the levels of Exo1. Nucleic Acids Res. 2017;45:6530-6545 pubmed publisher
    ..Here, we identify the Saccharomyces cerevisiae RNA binding protein Npl3 as a new player in DSB resection...