NOP7

Summary

Gene Symbol: NOP7
Description: mRNA-binding ribosome synthesis protein NOP7
Alias: YPH1, mRNA-binding ribosome synthesis protein NOP7
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Adams C, Jakovljevic J, Roman J, Harnpicharnchai P, Woolford J. Saccharomyces cerevisiae nucleolar protein Nop7p is necessary for biogenesis of 60S ribosomal subunits. RNA. 2002;8:150-65 pubmed
    ..We identified the gene NOP7that encodes an essential protein. The temperature-sensitive nop7-1 mutation or metabolic depletion of Nop7p results in a deficiency of 60S ribosomal subunits and accumulation of ..
  2. Merl J, Jakob S, Ridinger K, Hierlmeier T, Deutzmann R, Milkereit P, et al. Analysis of ribosome biogenesis factor-modules in yeast cells depleted from pre-ribosomes. Nucleic Acids Res. 2010;38:3068-80 pubmed publisher
    ..The data presented strengthen the idea that assembly of eukaryotic pre-ribosomal particles can result from transient association of distinct building blocks. ..
  3. Bassler J, Grandi P, Gadal O, Lessmann T, Petfalski E, Tollervey D, et al. Identification of a 60S preribosomal particle that is closely linked to nuclear export. Mol Cell. 2001;8:517-29 pubmed
    ..Eight of these proteins were copurified with nuclear pore complexes, suggesting that this complex represents the transport intermediate for 60S subunit export. ..
  4. Talkish J, Zhang J, Jakovljevic J, Horsey E, Woolford J. Hierarchical recruitment into nascent ribosomes of assembly factors required for 27SB pre-rRNA processing in Saccharomyces cerevisiae. Nucleic Acids Res. 2012;40:8646-61 pubmed
    ..Inhibition of TOR kinase decreases association of Rpf2, Spb4, Nog1 and Nog2 with pre-ribosomes. ..
  5. Dosil M, Bustelo X. Functional characterization of Pwp2, a WD family protein essential for the assembly of the 90 S pre-ribosomal particle. J Biol Chem. 2004;279:37385-97 pubmed
    ..Taken together, these results indicate that Pwp2 forms part of a stable particle subunit independent of the U3 small nucleolar ribonucleoprotein that is essential for the initial assembly steps of the 90 S pre-ribosome. ..
  6. Miles T, Jakovljevic J, Horsey E, Harnpicharnchai P, Tang L, Woolford J. Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes. Mol Cell Biol. 2005;25:10419-32 pubmed
    ..Ytm1 binds directly to Erb1 and is present in a heterotrimeric subcomplex together with Erb1 and Nop7, both within preribosomes and independently of preribosomes...
  7. Bassler J, Kallas M, Hurt E. The NUG1 GTPase reveals and N-terminal RNA-binding domain that is essential for association with 60 S pre-ribosomal particles. J Biol Chem. 2006;281:24737-44 pubmed
    ..Our findings suggest that Nug1 associates with pre-60 S subunits via its essential N-terminal RNA-binding domain and exerts a non-essential regulative role in pre-60 S subunit biogenesis via its central GTPase domain. ..
  8. Lebreton A, Saveanu C, Decourty L, Jacquier A, Fromont Racine M. Nsa2 is an unstable, conserved factor required for the maturation of 27 SB pre-rRNAs. J Biol Chem. 2006;281:27099-108 pubmed
    ..Indeed, when ribosome biogenesis was blocked upstream of Nsa2, this short half-lived protein was largely depleted, suggesting that its cellular levels are tightly regulated. ..
  9. Honma Y, Kitamura A, Shioda R, Maruyama H, Ozaki K, Oda Y, et al. TOR regulates late steps of ribosome maturation in the nucleoplasm via Nog1 in response to nutrients. EMBO J. 2006;25:3832-42 pubmed
    ..Nog1 formed a complex that included 60S ribosomal proteins and pre-ribosomal proteins Nop7 and Rlp24...

More Information

Publications28

  1. Harnpicharnchai P, Jakovljevic J, Horsey E, Miles T, Roman J, Rout M, et al. Composition and functional characterization of yeast 66S ribosome assembly intermediates. Mol Cell. 2001;8:505-15 pubmed
    ..These results demonstrate that proteomic approaches in concert with genetic tools provide powerful means to purify and characterize ribosome assembly intermediates. ..
  2. Tang L, Sahasranaman A, Jakovljevic J, Schleifman E, Woolford J. Interactions among Ytm1, Erb1, and Nop7 required for assembly of the Nop7-subcomplex in yeast preribosomes. Mol Biol Cell. 2008;19:2844-56 pubmed publisher
    ..The Nop7-subcomplex contains three assembly factors: Nop7, Erb1, and Ytm1, each of which is necessary for conversion of 27SA(..
  3. Lebreton A, Rousselle J, Lenormand P, Namane A, Jacquier A, Fromont Racine M, et al. 60S ribosomal subunit assembly dynamics defined by semi-quantitative mass spectrometry of purified complexes. Nucleic Acids Res. 2008;36:4988-99 pubmed publisher
    ..These results led to the characterisation of the composition of early, intermediate and late pre-ribosomal complexes, specific for crucial maturation steps during 60S assembly in eukaryotes. ..
  4. Granneman S, Petfalski E, Tollervey D. A cluster of ribosome synthesis factors regulate pre-rRNA folding and 5.8S rRNA maturation by the Rat1 exonuclease. EMBO J. 2011;30:4006-19 pubmed publisher
    ..Processing from A(3) requires the 'A(3)-cluster' proteins, including Cic1, Erb1, Nop7, Nop12 and Nop15, which show interdependent pre-rRNA binding...
  5. Oeffinger M, Leung A, Lamond A, Tollervey D, Lueng A. Yeast Pescadillo is required for multiple activities during 60S ribosomal subunit synthesis. RNA. 2002;8:626-36 pubmed
    ..Nop7p is a component of at least three different pre-60S particles, and we propose that it carries out distinct functions in each of these complexes. ..
  6. Pratte D, Singh U, Murat G, Kressler D. Mak5 and Ebp2 act together on early pre-60S particles and their reduced functionality bypasses the requirement for the essential pre-60S factor Nsa1. PLoS ONE. 2013;8:e82741 pubmed publisher
  7. Panse V, Kressler D, Pauli A, Petfalski E, Gnädig M, Tollervey D, et al. Formation and nuclear export of preribosomes are functionally linked to the small-ubiquitin-related modifier pathway. Traffic. 2006;7:1311-21 pubmed
    ..Together our data suggest that sumoylation of preribosomal particles in the nucleus and subsequent desumoylation at the NPC is necessary for efficient ribosome biogenesis and export in eukaryotes. ..
  8. Sakumoto N, Yamashita H, Mukai Y, Kaneko Y, Harashima S. Dual-specificity protein phosphatase Yvh1p, which is required for vegetative growth and sporulation, interacts with yeast pescadillo homolog in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2001;289:608-15 pubmed
    ..Two clones were identified encoding a segment of YPH1 (yeast pescadillo homolog), which is essential for cell cycle progression in yeast...
  9. Dembowski J, Kuo B, Woolford J. Has1 regulates consecutive maturation and processing steps for assembly of 60S ribosomal subunits. Nucleic Acids Res. 2013;41:7889-904 pubmed publisher
    ..Thus, our data support a model in which Has1 helps to establish domain I architecture to prevent pre-rRNA turnover and couples domain I folding with consecutive pre-rRNA processing steps. ..
  10. Matsuo Y, Granneman S, Thoms M, Manikas R, Tollervey D, Hurt E. Coupled GTPase and remodelling ATPase activities form a checkpoint for ribosome export. Nature. 2014;505:112-116 pubmed publisher
    ..We conclude that Nug2 is a regulatory GTPase that monitors pre-60S maturation, with release from its placeholder site linked to recruitment of the nuclear export machinery. ..
  11. Rodríguez Galán O, García Gómez J, Kressler D, de la Cruz J. Immature large ribosomal subunits containing the 7S pre-rRNA can engage in translation in Saccharomyces cerevisiae. RNA Biol. 2015;12:838-46 pubmed publisher
    ..8s rrna. ..
  12. Trahan C, Oeffinger M. Targeted cross-linking-mass spectrometry determines vicinal interactomes within heterogeneous RNP complexes. Nucleic Acids Res. 2016;44:1354-69 pubmed publisher
    ..RNA-associated complexes in Saccharomyces cerevisae, the mRNA export receptor Mex67:Mtr2 and the pre-ribosomal Nop7 subcomplex, we identified dynamic vicinal interactomes within those complexes and along their changing pathway ..
  13. Martin Marcos P, Hinnebusch A, Tamame M. Ribosomal protein L33 is required for ribosome biogenesis, subunit joining, and repression of GCN4 translation. Mol Cell Biol. 2007;27:5968-85 pubmed
    ..We further demonstrate that rpl33a-G76R impairs the efficient processing of 35S and 27S pre-rRNAs and reduces the accumulation of all four mature rRNAs, indicating an important role for L33 in the biogenesis of both ribosomal subunits. ..
  14. Killian A, Le Meur N, Sesboué R, Bourguignon J, Bougeard G, Gautherot J, et al. Inactivation of the RRB1-Pescadillo pathway involved in ribosome biogenesis induces chromosomal instability. Oncogene. 2004;23:8597-602 pubmed
    ..We demonstrate that RRB1 interacts with YPH1 (yeast pescadillo homologue 1) and other members of the Yph1 complex, RPL3, ERB1 and ORC6, involved in ribosome ..
  15. Yao Y, Demoinet E, Saveanu C, Lenormand P, Jacquier A, Fromont Racine M. Ecm1 is a new pre-ribosomal factor involved in pre-60S particle export. RNA. 2010;16:1007-17 pubmed publisher
    ..These data highlight the involvement of many factors acting together to export pre-60S particles. ..
  16. Fernández Pévida A, Rodríguez Galán O, Díaz Quintana A, Kressler D, de la Cruz J. Yeast ribosomal protein L40 assembles late into precursor 60 S ribosomes and is required for their cytoplasmic maturation. J Biol Chem. 2012;287:38390-407 pubmed publisher
  17. Sarkar A, Pech M, Thoms M, Beckmann R, Hurt E. Ribosome-stalk biogenesis is coupled with recruitment of nuclear-export factor to the nascent 60S subunit. Nat Struct Mol Biol. 2016;23:1074-1082 pubmed publisher
    ..Thus, a spatiotemporal mark on the ribosomal stalk controls the recruitment of an RNA-export receptor to the nascent 60S subunit. ..
  18. Daoud R, Forget L, Lang B. Yeast mitochondrial RNase P, RNase Z and the RNA degradosome are part of a stable supercomplex. Nucleic Acids Res. 2012;40:1728-36 pubmed publisher
    ..Finally, the question of mt-RNase P localization within mitochondria was investigated, by GFP-tracing of a known protein subunit (Rpm2p). We find that about equal fractions of RNase P are soluble versus membrane-attached. ..
  19. Francisco Velilla R, Remacha M, Ballesta J. Carboxy terminal modifications of the P0 protein reveal alternative mechanisms of nuclear ribosomal stalk assembly. Nucleic Acids Res. 2013;41:8628-36 pubmed publisher
    ..Furthermore, in wild-type cells, the presence of nuclear pre-ribosome complexes containing P0 but not L12 is compatible with the existence of an alternative stalk assembly process. ..