Gene Symbol: NOP4
Description: mRNA-binding ribosome biosynthesis protein NOP4
Alias: NOP77, mRNA-binding ribosome biosynthesis protein NOP4
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Sun C, Woolford J. The yeast NOP4 gene product is an essential nucleolar protein required for pre-rRNA processing and accumulation of 60S ribosomal subunits. EMBO J. 1994;13:3127-35 pubmed
    The Saccharomyces cerevisiae NOP4 gene was isolated by screening a lambda gt11 yeast genomic DNA library with a monoclonal antibody against a yeast nucleolar protein...
  2. Berges T, Petfalski E, Tollervey D, Hurt E. Synthetic lethality with fibrillarin identifies NOP77p, a nucleolar protein required for pre-rRNA processing and modification. EMBO J. 1994;13:3136-48 pubmed
    ..mutations showing synthetic lethality with a temperature sensitive nop1-5 allele led to the identification of the NOP77 gene. NOP77 is essential for viability and encodes a nucleolar protein with a predicted molecular weight of 77 kDa...
  3. Venema J, Tollervey D. Ribosome synthesis in Saccharomyces cerevisiae. Annu Rev Genet. 1999;33:261-311 pubmed
    ..Here, we will discuss the recent, and often surprising, advances in our understanding of ribosome synthesis in the yeast Saccharomyces cerevisiae. These will underscore the unexpected complexity of eukaryotic ribosome synthesis. ..
  4. Miles T, Jakovljevic J, Horsey E, Harnpicharnchai P, Tang L, Woolford J. Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes. Mol Cell Biol. 2005;25:10419-32 pubmed
    ..Mutations in the WD40 motifs of Ytm1 disrupt binding to Erb1, destabilize the heterotrimer, and delay pre-rRNA processing and nuclear export of preribosomes. Nevertheless, 66S preribosomes lacking Ytm1 remain otherwise intact. ..
  5. Granneman S, Petfalski E, Tollervey D. A cluster of ribosome synthesis factors regulate pre-rRNA folding and 5.8S rRNA maturation by the Rat1 exonuclease. EMBO J. 2011;30:4006-19 pubmed publisher
    ..In contrast, Nop4, a protein required for endonucleolytic cleavage in ITS1, binds the pre-rRNA near the 5'-end of 5.8S...
  6. Shimoji K, Jakovljevic J, Tsuchihashi K, Umeki Y, Wan K, Kawasaki S, et al. Ebp2 and Brx1 function cooperatively in 60S ribosomal subunit assembly in Saccharomyces cerevisiae. Nucleic Acids Res. 2012;40:4574-88 pubmed publisher
  7. Sun C, Woolford J. The yeast nucleolar protein Nop4p contains four RNA recognition motifs necessary for ribosome biogenesis. J Biol Chem. 1997;272:25345-52 pubmed
    ..To examine the functional importance of these motifs, we isolated random and site-directed mutations in NOP4 and assayed Nop4p function in vivo...
  8. Gautier T, Berges T, Tollervey D, Hurt E. Nucleolar KKE/D repeat proteins Nop56p and Nop58p interact with Nop1p and are required for ribosome biogenesis. Mol Cell Biol. 1997;17:7088-98 pubmed
    ..Well-conserved homologs are present in a range of organisms, including humans (52% identity between human hNop56p and yeast Nop56p), suggesting that these complexes have been conserved in evolution. ..
  9. Pratte D, Singh U, Murat G, Kressler D. Mak5 and Ebp2 act together on early pre-60S particles and their reduced functionality bypasses the requirement for the essential pre-60S factor Nsa1. PLoS ONE. 2013;8:e82741 pubmed publisher
    ..of genes encoding the DEAD-box RNA helicase Mak5, the C/D-box snoRNP component Nop1 and the rRNA-binding protein Nop4 bypass the requirement for Nsa1...

More Information


  1. McCann K, Teramoto T, Zhang J, Tanaka Hall T, Baserga S. The molecular basis for ANE syndrome revealed by the large ribosomal subunit processome interactome. elife. 2016;5: pubmed publisher
    ..a ribosomopathy caused by a mutation in an RNA recognition motif of RBM28, a nucleolar protein conserved to yeast (Nop4)...