Gene Symbol: NOP1
Description: rRNA methyltransferase NOP1
Alias: LOT3, rRNA methyltransferase NOP1
Species: Saccharomyces cerevisiae S288c
Products:     NOP1

Top Publications

  1. Gonzales F, Zanchin N, Luz J, Oliveira C. Characterization of Saccharomyces cerevisiae Nop17p, a novel Nop58p-interacting protein that is involved in Pre-rRNA processing. J Mol Biol. 2005;346:437-55 pubmed
  2. Tollervey D, Lehtonen H, Jansen R, Kern H, Hurt E. Temperature-sensitive mutations demonstrate roles for yeast fibrillarin in pre-rRNA processing, pre-rRNA methylation, and ribosome assembly. Cell. 1993;72:443-57 pubmed
    ..lethal point mutations in the small nucleolar RNA-associated protein fibrillarin (encoded by the NOP1 gene in yeast) and analyzed their effects on ribosome synthesis...
  3. Billy E, Wegierski T, Nasr F, Filipowicz W. Rcl1p, the yeast protein similar to the RNA 3'-phosphate cyclase, associates with U3 snoRNP and is required for 18S rRNA biogenesis. EMBO J. 2000;19:2115-26 pubmed
    ..Possible functions of Rcl1p in pre-rRNA processing and its relationship to the RNA 3'-phosphate cyclase are discussed. ..
  4. Boulon S, Marmier Gourrier N, Pradet Balade B, Wurth L, Verheggen C, Jady B, et al. The Hsp90 chaperone controls the biogenesis of L7Ae RNPs through conserved machinery. J Cell Biol. 2008;180:579-95 pubmed publisher
    ..Thus, Hsp90 may control the folding of these proteins during the formation of new RNPs. This suggests that Hsp90 functions as a master regulator of cell proliferation by allowing simultaneous control of cell signaling and cell growth. ..
  5. Chen W, Bucaria J, Band D, Sutton A, Sternglanz R. Enp1, a yeast protein associated with U3 and U14 snoRNAs, is required for pre-rRNA processing and 40S subunit synthesis. Nucleic Acids Res. 2003;31:690-9 pubmed
    ..Co-precipitation analysis revealed that Enp1 was associated with Nop1 protein, as well as with U3 and U14 RNAs, two snoRNAs implicated in early pre-rRNA processing steps...
  6. Galardi S, Fatica A, Bachi A, Scaloni A, Presutti C, Bozzoni I. Purified box C/D snoRNPs are able to reproduce site-specific 2'-O-methylation of target RNA in vitro. Mol Cell Biol. 2002;22:6663-8 pubmed
    ..We show that purified snoRNPs are able to reproduce the site-specific methylation pattern on target RNA and that the predicted S-adenosyl-L-methionine-binding region of Nop1p is responsible for the catalytic activity. ..
  7. Gallagher J, Dunbar D, Granneman S, Mitchell B, Osheim Y, Beyer A, et al. RNA polymerase I transcription and pre-rRNA processing are linked by specific SSU processome components. Genes Dev. 2004;18:2506-17 pubmed
    ..This association may thus represent an early step in the formation of the SSU processome. Our results indicate that rRNA transcription and pre-rRNA processing are coordinated via specific components of the SSU processome. ..
  8. Lafontaine D, Tollervey D. Synthesis and assembly of the box C+D small nucleolar RNPs. Mol Cell Biol. 2000;20:2650-9 pubmed
    ..Unexpectedly, we found that Nop1p was specifically required for the synthesis and accumulation of box C+D snoRNAs processed from pre-mRNA introns and polycistronic transcripts. ..
  9. Berges T, Petfalski E, Tollervey D, Hurt E. Synthetic lethality with fibrillarin identifies NOP77p, a nucleolar protein required for pre-rRNA processing and modification. EMBO J. 1994;13:3136-48 pubmed
    The nucleolar protein fibrillarin (encoded by the NOP1 gene in yeast), is required for many post-transcriptional steps in yeast ribosome synthesis...

More Information


  1. Gautier T, Berges T, Tollervey D, Hurt E. Nucleolar KKE/D repeat proteins Nop56p and Nop58p interact with Nop1p and are required for ribosome biogenesis. Mol Cell Biol. 1997;17:7088-98 pubmed
    ..A screen for mutations that are synthetically lethal (sl) with the nop1-5 allele, which inhibits pre-rRNA processing, identified NOP56...
  2. Fath S, Milkereit P, Podtelejnikov A, Bischler N, Schultz P, Bier M, et al. Association of yeast RNA polymerase I with a nucleolar substructure active in rRNA synthesis and processing. J Cell Biol. 2000;149:575-90 pubmed
    ..Our results support the idea that a functional nucleolar subdomain formed independently of the state of rDNA transcription may serve as a scaffold for coordinated rRNA synthesis and processing. ..
  3. Watkins N, Ségault V, Charpentier B, Nottrott S, Fabrizio P, Bachi A, et al. A common core RNP structure shared between the small nucleoar box C/D RNPs and the spliceosomal U4 snRNP. Cell. 2000;103:457-66 pubmed
    ..5 kD in vitro. The similarities in structure and function observed between the U4 snRNP (chaperone for U6) and the box C/D snoRNPs raises the interesting possibility that these particles may have evolved from a common ancestral RNP. ..
  4. Dosil M, Bustelo X. Functional characterization of Pwp2, a WD family protein essential for the assembly of the 90 S pre-ribosomal particle. J Biol Chem. 2004;279:37385-97 pubmed
    ..Taken together, these results indicate that Pwp2 forms part of a stable particle subunit independent of the U3 small nucleolar ribonucleoprotein that is essential for the initial assembly steps of the 90 S pre-ribosome. ..
  5. Pintard L, Kressler D, Lapeyre B. Spb1p is a yeast nucleolar protein associated with Nop1p and Nop58p that is able to bind S-adenosyl-L-methionine in vitro. Mol Cell Biol. 2000;20:1370-81 pubmed
    ..We show here that Spb1p is able to bind [(3)H]AdoMet in vitro, suggesting that it is a novel methylase, whose possible substrates will be discussed. ..
  6. Straight A, Shou W, Dowd G, Turck C, Deshaies R, Johnson A, et al. Net1, a Sir2-associated nucleolar protein required for rDNA silencing and nucleolar integrity. Cell. 1999;97:245-56 pubmed
    ..Our findings suggest that a protein complex shown to regulate exit from mitosis is also involved in gene silencing. ..
  7. Soltanieh S, Osheim Y, Spasov K, Trahan C, Beyer A, Dragon F. DEAD-box RNA helicase Dbp4 is required for small-subunit processome formation and function. Mol Cell Biol. 2015;35:816-30 pubmed publisher
    ..We also found that the C-terminal extension flanking the catalytic core of Dbp4 plays an important role in the release of U14 snoRNA from pre-rRNA. ..
  8. Dez C, Noaillac Depeyre J, Caizergues Ferrer M, Henry Y. Naf1p, an essential nucleoplasmic factor specifically required for accumulation of box H/ACA small nucleolar RNPs. Mol Cell Biol. 2002;22:7053-65 pubmed
    ..Naf1p is unlikely to be directly required for the synthesis of H/ACA snoRNP components. Naf1p could participate in H/ACA snoRNP assembly and/or transport. ..
  9. Skowronek E, Grzechnik P, Späth B, Marchfelder A, Kufel J. tRNA 3' processing in yeast involves tRNase Z, Rex1, and Rrp6. RNA. 2014;20:115-30 pubmed publisher
    ..In addition to its function in the nucleus, Trz1 processes the 3' ends of mitochondrial tRNAs, contributing to the general RNA metabolism in this organelle. ..
  10. Gerus M, Bonnart C, Caizergues Ferrer M, Henry Y, Henras A. Evolutionarily conserved function of RRP36 in early cleavages of the pre-rRNA and production of the 40S ribosomal subunit. Mol Cell Biol. 2010;30:1130-44 pubmed publisher
    ..In parallel, we studied the human orthologue of Rrp36p in HeLa cells, and we show that the function of this protein in early cleavages of the pre-rRNA has been conserved through evolution in eukaryotes. ..
  11. Hsieh C, Huang S, Wu Y, Liu L, Han C, Liu Y, et al. Expression of proteins with dimethylarginines in Escherichia coli for protein-protein interaction studies. Protein Sci. 2007;16:919-28 pubmed
    ..Sbp1p and Sbp1p/hmt1 were covalently attached to solid supports for the isolation of interacting proteins. The results indicate that arginine methylation on Sbp1p exerts both positive and negative effects on protein-protein interaction. ..
  12. Morlando M, Ballarino M, Greco P, Caffarelli E, Dichtl B, Bozzoni I. Coupling between snoRNP assembly and 3' processing controls box C/D snoRNA biosynthesis in yeast. EMBO J. 2004;23:2392-401 pubmed
    ..These data suggest a mechanism of quality control in which efficient transcription and 3'-end formation occur only when nascent snoRNAs are successfully assembled into functional particles. ..
  13. Hoang T, Peng W, Vanrobays E, Krogan N, Hiley S, Beyer A, et al. Esf2p, a U3-associated factor required for small-subunit processome assembly and compaction. Mol Cell Biol. 2005;25:5523-34 pubmed
    ..The identification of ABT1 in a large-scale analysis of the human nucleolar proteome indicates that its role may also be conserved in mammals. ..
  14. Leslie D, Zhang W, Timney B, Chait B, Rout M, Wozniak R, et al. Characterization of karyopherin cargoes reveals unique mechanisms of Kap121p-mediated nuclear import. Mol Cell Biol. 2004;24:8487-503 pubmed
    ..Together, our data elucidate additional levels of complexity in these nuclear transport pathways. ..
  15. Tollervey D, Lehtonen H, Carmo Fonseca M, Hurt E. The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast. EMBO J. 1991;10:573-83 pubmed
    The yeast snoRNP protein, NOP1, is structurally and functionally homologous to vertebrate fibrillarin and is essential for viability...
  16. Yagoub D, Hart Smith G, Moecking J, Erce M, Wilkins M. Yeast proteins Gar1p, Nop1p, Npl3p, Nsr1p, and Rps2p are natively methylated and are substrates of the arginine methyltransferase Hmt1p. Proteomics. 2015;15:3209-18 pubmed publisher
    ..This brings the total of Hmt1 substrate proteins for which native methylation sites have been identified to five. ..
  17. Fernández Pévida A, Rodríguez Galán O, Díaz Quintana A, Kressler D, de la Cruz J. Yeast ribosomal protein L40 assembles late into precursor 60 S ribosomes and is required for their cytoplasmic maturation. J Biol Chem. 2012;287:38390-407 pubmed publisher
  18. Tessarz P, Santos Rosa H, Robson S, Sylvestersen K, Nelson C, Nielsen M, et al. Glutamine methylation in histone H2A is an RNA-polymerase-I-dedicated modification. Nature. 2014;505:564-8 pubmed publisher
    ..We identify Nop1 as the methyltransferase in yeast and demonstrate that fibrillarin is the orthologue enzyme in human cells...
  19. Moriggi G, Nieto B, Dosil M. Rrp12 and the Exportin Crm1 participate in late assembly events in the nucleolus during 40S ribosomal subunit biogenesis. PLoS Genet. 2014;10:e1004836 pubmed publisher
  20. Gemayel R, Chavali S, Pougach K, Legendre M, Zhu B, Boeynaems S, et al. Variable Glutamine-Rich Repeats Modulate Transcription Factor Activity. Mol Cell. 2015;59:615-27 pubmed publisher
    ..Thus, Q-rich repeats are dynamic functional domains that modulate a regulator's innate function, with the inherent risk of pathogenic repeat expansions. ..
  21. Xu C, Henry P, Setya A, Henry M. In vivo analysis of nucleolar proteins modified by the yeast arginine methyltransferase Hmt1/Rmt1p. RNA. 2003;9:746-59 pubmed
    In this report, we have investigated the impact of arginine methylation on the Gar1, Nop1, and Nsr1 nucleolar proteins in Saccharomyces cerevisiae...
  22. Rabitsch K, Petronczki M, Javerzat J, Genier S, Chwalla B, Schleiffer A, et al. Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I. Dev Cell. 2003;4:535-48 pubmed
    ..Csm1 and Pcs1 might clamp together microtubule binding sites on the same (Pcs1) or sister (Csm1) kinetochores...
  23. Jansen R, Tollervey D, Hurt E. A U3 snoRNP protein with homology to splicing factor PRP4 and G beta domains is required for ribosomal RNA processing. EMBO J. 1993;12:2549-58 pubmed
    Yeast fibrillarin (NOP1) is an evolutionarily conserved, nucleolar protein necessary for multiple steps in ribosome biogenesis...
  24. Schimmang T, Tollervey D, Kern H, Frank R, Hurt E. A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability. EMBO J. 1989;8:4015-24 pubmed
    ..We have identified a major 38 kd nucleolar protein (NOP1), which is located within nucleolar structures resembling the dense fibrillar region of mammalian nucleoli...
  25. Lempiäinen H, Uotila A, Urban J, Dohnal I, Ammerer G, Loewith R, et al. Sfp1 interaction with TORC1 and Mrs6 reveals feedback regulation on TOR signaling. Mol Cell. 2009;33:704-16 pubmed publisher
    ..Finally, we show that the Sfp1-interacting protein Mrs6, a Rab escort protein involved in membrane trafficking, regulates both Sfp1 nuclear localization and TORC1 signaling. ..
  26. Philippi A, Steinbauer R, Reiter A, Fath S, Leger Silvestre I, Milkereit P, et al. TOR-dependent reduction in the expression level of Rrn3p lowers the activity of the yeast RNA Pol I machinery, but does not account for the strong inhibition of rRNA production. Nucleic Acids Res. 2010;38:5315-26 pubmed publisher
    ..However, our analysis reveals that the dramatic reduction of rRNA synthesis in the immediate cellular response to impaired TOR signalling cannot be explained by the simple down-regulation of Rrn3p and Pol I-Rrn3p levels. ..
  27. Toh e A, Oguchi T. Genetic characterization of genes encoding enzymes catalyzing addition of phospho-ethanolamine to the glycosylphosphatidylinositol anchor in Saccharomyces cerevisiae. Genes Genet Syst. 2002;77:309-22 pubmed
    ..Las21 did not show preference for the metabolic pathway to produce phosphatidylethanolamine. ..
  28. Pratte D, Singh U, Murat G, Kressler D. Mak5 and Ebp2 act together on early pre-60S particles and their reduced functionality bypasses the requirement for the essential pre-60S factor Nsa1. PLoS ONE. 2013;8:e82741 pubmed publisher
    ..Here we show that mutant alleles of genes encoding the DEAD-box RNA helicase Mak5, the C/D-box snoRNP component Nop1 and the rRNA-binding protein Nop4 bypass the requirement for Nsa1...
  29. Moy T, Boettner D, Rhodes J, Silver P, Askew D. Identification of a role for Saccharomyces cerevisiae Cgr1p in pre-rRNA processing and 60S ribosome subunit synthesis. Microbiology. 2002;148:1081-90 pubmed
    ..Together these findings establish a role for Cgr1p in ribosome biogenesis. ..
  30. Kakihara Y, Makhnevych T, Zhao L, Tang W, Houry W. Nutritional status modulates box C/D snoRNP biogenesis by regulated subcellular relocalization of the R2TP complex. Genome Biol. 2014;15:404 pubmed publisher
    Box C/D snoRNPs, which are typically composed of box C/D snoRNA and the four core protein components Nop1, Nop56, Nop58, and Snu13, play an essential role in the modification and processing of pre-ribosomal RNA...
  31. Rothé B, Saliou J, Quinternet M, Back R, Tiotiu D, Jacquemin C, et al. Protein Hit1, a novel box C/D snoRNP assembly factor, controls cellular concentration of the scaffolding protein Rsa1 by direct interaction. Nucleic Acids Res. 2014;42:10731-47 pubmed publisher
    ..Our biochemical data show that C/D snoRNAs and the core protein Nop58 can interact with the purified Snu13p-Rsa1p-Hit1p heterotrimer. ..
  32. Giorgi C, Fatica A, Nagel R, Bozzoni I. Release of U18 snoRNA from its host intron requires interaction of Nop1p with the Rnt1p endonuclease. EMBO J. 2001;20:6856-65 pubmed
    ..These data indicate that cleavage of U18, snR38 and possibly other intron-encoded snoRNAs is a regulated process, since the stem is cleaved by the Rnt1p endonuclease only when snoRNP assembly has occurred. ..
  33. Lefebvre O, Ruth J, Sentenac A. A mutation in the largest subunit of yeast TFIIIC affects tRNA and 5 S RNA synthesis. Identification of two classes of suppressors. J Biol Chem. 1994;269:23374-81 pubmed
    ..A second class of suppressors is not involved in transcription but alleviates the main physiological defects of mutant cells. It includes RPR1 and NOP1, required for the maturation of pre-tRNA and pre-rRNA, respectively.
  34. Peng W, Krogan N, Richards D, Greenblatt J, Hughes T. ESF1 is required for 18S rRNA synthesis in Saccharomyces cerevisiae. Nucleic Acids Res. 2004;32:1993-9 pubmed
    ..These data indicate that Esf1p plays a direct role in early pre-rRNA processing. ..
  35. Granato D, Machado Santelli G, Oliveira C. Nop53p interacts with 5.8S rRNA co-transcriptionally, and regulates processing of pre-rRNA by the exosome. FEBS J. 2008;275:4164-78 pubmed publisher
    ..Consistent with this observation and similar to the observed in exosome mutants, depletion of Nop53p leads to accumulation of polyadenylated pre-rRNAs. ..
  36. Kammerer D, Stevermann L, Liakopoulos D. Ubiquitylation regulates interactions of astral microtubules with the cleavage apparatus. Curr Biol. 2010;20:1233-43 pubmed publisher
    ..Photoconversion experiments showed that Kar9 association with aMTs is stable. We propose that ubiquitylation controls interactions of aMTs with the cleavage apparatus through localized disassembly of Kar9 complexes. ..