Nmd3p

Summary

Gene Symbol: Nmd3p
Description: ribosome-binding protein NMD3
Alias: SRC5, ribosome-binding protein NMD3
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Belk J, He F, Jacobson A. Overexpression of truncated Nmd3p inhibits protein synthesis in yeast. RNA. 1999;5:1055-70 pubmed
    ..Overexpression of a truncated form of Nmd3p, lacking 100 C-terminal amino acids and most of its Upf1p-interacting domain, had dominant-negative effects on ..
  2. Bussiere C, Hashem Y, Arora S, Frank J, Johnson A. Integrity of the P-site is probed during maturation of the 60S ribosomal subunit. J Cell Biol. 2012;197:747-59 pubmed publisher
    ..We propose that Efl1 promotes a functional check of the integrity of the 60S subunit before its first round of translation. ..
  3. Hackmann A, Gross T, Baierlein C, Krebber H. The mRNA export factor Npl3 mediates the nuclear export of large ribosomal subunits. EMBO Rep. 2011;12:1024-31 pubmed publisher
    ..Mutations in NPL3 lead to export defects of the large subunit and genetic interactions with other pre-60S export factors...
  4. West M, Hedges J, Lo K, Johnson A. Novel interaction of the 60S ribosomal subunit export adapter Nmd3 at the nuclear pore complex. J Biol Chem. 2007;282:14028-37 pubmed
    ..These results suggest that in the absence of the ribosome, Nmd3 is not efficiently released from Crm1 at the NPC. ..
  5. Lo K, Johnson A. Reengineering ribosome export. Mol Biol Cell. 2009;20:1545-54 pubmed publisher
    ..These results imply remarkable flexibility in the export pathway for the 60S subunit and help explain how different export receptors could have evolved in different eukaryotic lineages. ..
  6. Ho J, Johnson A. NMD3 encodes an essential cytoplasmic protein required for stable 60S ribosomal subunits in Saccharomyces cerevisiae. Mol Cell Biol. 1999;19:2389-99 pubmed
    ..However, the mature RNA was rapidly degraded, with a half-life of 4 min. Nmd3p fractionated as a cytoplasmic protein and sedimented in the position of free 60S subunits in sucrose gradients...
  7. Zuk D, Belk J, Jacobson A. Temperature-sensitive mutations in the Saccharomyces cerevisiae MRT4, GRC5, SLA2 and THS1 genes result in defects in mRNA turnover. Genetics. 1999;153:35-47 pubmed
    ..The growth and mRNA decay defects of ts942 (grc5) cells were suppressed by overexpression of the NMD3 gene, encoding a protein shown to participate in a two-hybrid interaction with the nonsense-mediated decay protein Upf1p. ..
  8. Ho J, Kallstrom G, Johnson A. Nascent 60S ribosomal subunits enter the free pool bound by Nmd3p. RNA. 2000;6:1625-34 pubmed
    b>Nmd3p from yeast is required for the export of the large (60S) ribosomal subunit from the nucleus (Ho et al., 2000). Here, we show that Nmd3p forms a stable complex with free 60S subunits...
  9. Gadal O, Strauss D, Kessl J, Trumpower B, Tollervey D, Hurt E. Nuclear export of 60s ribosomal subunits depends on Xpo1p and requires a nuclear export sequence-containing factor, Nmd3p, that associates with the large subunit protein Rpl10p. Mol Cell Biol. 2001;21:3405-15 pubmed
    ..One of these corresponds to the ribosomal protein Rpl10p, which interacts directly with Nmd3p, a conserved and essential protein associated with 60S subunits...

More Information

Publications36

  1. Hedges J, West M, Johnson A. Release of the export adapter, Nmd3p, from the 60S ribosomal subunit requires Rpl10p and the cytoplasmic GTPase Lsg1p. EMBO J. 2005;24:567-79 pubmed
    In eukaryotes, nuclear export of the large (60S) ribosomal subunit requires the adapter protein Nmd3p to provide the nuclear export signal...
  2. Hofer A, Bussiere C, Johnson A. Mutational analysis of the ribosomal protein Rpl10 from yeast. J Biol Chem. 2007;282:32630-9 pubmed
    ..We also found that mutant Rpl10 proteins were engineered to be unable to bind to the ribosome accumulated in the nucleus. This was unexpected and may suggest a nuclear role for Rpl10. ..
  3. Hung N, Lo K, Patel S, Helmke K, Johnson A. Arx1 is a nuclear export receptor for the 60S ribosomal subunit in yeast. Mol Biol Cell. 2008;19:735-44 pubmed
    ..These results show that Arx1 can directly bridge the interaction between the pre-60S particle and the NPC and thus is a third export receptor for the 60S subunit in yeast. ..
  4. Lo K, Li Z, Wang F, Marcotte E, Johnson A. Ribosome stalk assembly requires the dual-specificity phosphatase Yvh1 for the exchange of Mrt4 with P0. J Cell Biol. 2009;186:849-62 pubmed publisher
    ..The initial assembly of the ribosome with Mrt4 may provide functional compartmentalization of ribosome assembly in addition to the spatial separation afforded by the nuclear envelope. ..
  5. Lo K, Li Z, Bussiere C, Bresson S, Marcotte E, Johnson A. Defining the pathway of cytoplasmic maturation of the 60S ribosomal subunit. Mol Cell. 2010;39:196-208 pubmed publisher
    ..Finally, the release of Tif6 is a prerequisite for the release of the nuclear export adaptor Nmd3. Establishing this pathway provides an important conceptual framework for understanding ribosome maturation. ..
  6. Sengupta J, Bussiere C, Pallesen J, West M, Johnson A, Frank J. Characterization of the nuclear export adaptor protein Nmd3 in association with the 60S ribosomal subunit. J Cell Biol. 2010;189:1079-86 pubmed publisher
    ..This cryo-EM map is a first step toward a molecular understanding of the functional role and release mechanism of Nmd3. ..
  7. Yao Y, Demoinet E, Saveanu C, Lenormand P, Jacquier A, Fromont Racine M. Ecm1 is a new pre-ribosomal factor involved in pre-60S particle export. RNA. 2010;16:1007-17 pubmed publisher
    ..These data highlight the involvement of many factors acting together to export pre-60S particles. ..
  8. Kallstrom G, Hedges J, Johnson A. The putative GTPases Nog1p and Lsg1p are required for 60S ribosomal subunit biogenesis and are localized to the nucleus and cytoplasm, respectively. Mol Cell Biol. 2003;23:4344-55 pubmed
    ..that are found associated with free 60S ribosomal subunits affinity purified with the nuclear export adapter Nmd3p. Nog1p and Lsg1p are nucleolar and cytoplasmic, respectively, and are not simultaneously on the same particle, ..
  9. Ho J, Kallstrom G, Johnson A. Nmd3p is a Crm1p-dependent adapter protein for nuclear export of the large ribosomal subunit. J Cell Biol. 2000;151:1057-66 pubmed
    ..We showed previously that Nmd3p is an essential protein from yeast that is required for a late step in biogenesis of the large (60S) ribosomal ..
  10. Hedges J, Chen Y, West M, Bussiere C, Johnson A. Mapping the functional domains of yeast NMD3, the nuclear export adapter for the 60 S ribosomal subunit. J Biol Chem. 2006;281:36579-87 pubmed
    Nuclear export of the large ribosomal subunit requires the adapter protein Nmd3p to provide a leucine-rich nuclear export signal that is recognized by the export receptor Crm1. Nmd3p binds to the pre-60 S subunit in the nucleus...
  11. West M, Hedges J, Chen A, Johnson A. Defining the order in which Nmd3p and Rpl10p load onto nascent 60S ribosomal subunits. Mol Cell Biol. 2005;25:3802-13 pubmed
    ..that Rpl10p as well as the cytoplasmic GTPase Lsg1p are required for releasing the 60S nuclear export adapter Nmd3p from subunits in the cytoplasm...
  12. Malyutin A, Musalgaonkar S, Patchett S, Frank J, Johnson A. Nmd3 is a structural mimic of eIF5A, and activates the cpGTPase Lsg1 during 60S ribosome biogenesis. EMBO J. 2017;36:854-868 pubmed publisher
    ..Nmd3 and Lsg1 together embrace helix 69 of the B2a intersubunit bridge, inducing base flipping that we suggest may activate the GTPase activity of Lsg1. ..
  13. Karl T, Onder K, Kodzius R, Pichova A, Wimmer H, Th r A, et al. GRC5 and NMD3 function in translational control of gene expression and interact genetically. Curr Genet. 1999;34:419-29 pubmed
    ..NMD3 was previously identified as a component of the nonsense-mediated mRNA decay pathway. The point mutations within NMD3 reported here may define a domain important for the functional interaction of Grc5p and Nmd3p.
  14. Merl J, Jakob S, Ridinger K, Hierlmeier T, Deutzmann R, Milkereit P, et al. Analysis of ribosome biogenesis factor-modules in yeast cells depleted from pre-ribosomes. Nucleic Acids Res. 2010;38:3068-80 pubmed publisher
    ..The data presented strengthen the idea that assembly of eukaryotic pre-ribosomal particles can result from transient association of distinct building blocks. ..
  15. Neumann B, Wu H, Hackmann A, Krebber H. Nuclear Export of Pre-Ribosomal Subunits Requires Dbp5, but Not as an RNA-Helicase as for mRNA Export. PLoS ONE. 2016;11:e0149571 pubmed publisher
    ..Thus, our findings reveal that although different ribonucleoparticles, mRNAs and pre-ribosomal subunits, use shared export factors, they utilize different transport mechanisms. ..
  16. Occhipinti L, Chang Y, Altvater M, Menet A, Kemmler S, Panse V. Non-FG mediated transport of the large pre-ribosomal subunit through the nuclear pore complex by the mRNA export factor Gle2. Nucleic Acids Res. 2013;41:8266-79 pubmed publisher
    ..We propose that large complex cargos rely on non-FG as well as FG-interactions for their efficient translocation through the nuclear pore complex channel. ..
  17. Loibl M, Klein I, Prattes M, Schmidt C, Kappel L, Zisser G, et al. The drug diazaborine blocks ribosome biogenesis by inhibiting the AAA-ATPase Drg1. J Biol Chem. 2014;289:3913-22 pubmed publisher
    ..Our results identify the first target of an inhibitor of ribosome biogenesis and provide the mechanism of inhibition of a key step in large ribosomal subunit formation. ..
  18. Parnell K, Bass B. Functional redundancy of yeast proteins Reh1 and Rei1 in cytoplasmic 60S subunit maturation. Mol Cell Biol. 2009;29:4014-23 pubmed publisher
    ..We observe that strains lacking both Reh1 and Rei1 accumulate salt-labile 60S subunits, suggesting that Reh1/Rei1 is necessary for the cytoplasmic 60S subunit to adopt its mature, stable form. ..
  19. Ma C, Wu S, Li N, Chen Y, Yan K, Li Z, et al. Structural snapshot of cytoplasmic pre-60S ribosomal particles bound by Nmd3, Lsg1, Tif6 and Reh1. Nat Struct Mol Biol. 2017;24:214-220 pubmed publisher
    ..These findings pinpoint a structural checkpoint role for Nmd3 in PTC assembly, and provide information about functional and mechanistic roles of these assembly factors in the maturation of the 60S ribosomal subunit. ..
  20. Sulima S, Patchett S, Advani V, De Keersmaecker K, Johnson A, Dinman J. Bypass of the pre-60S ribosomal quality control as a pathway to oncogenesis. Proc Natl Acad Sci U S A. 2014;111:5640-5 pubmed publisher
  21. Albanèse V, Reissmann S, Frydman J. A ribosome-anchored chaperone network that facilitates eukaryotic ribosome biogenesis. J Cell Biol. 2010;189:69-81 pubmed publisher
    ..Our results demonstrate that, in addition to their known cytoplasmic roles in de novo protein folding, some ribosome-anchored CLIPS chaperones play a critical role in nuclear steps of ribosome biogenesis. ..
  22. Yao W, Lutzmann M, Hurt E. A versatile interaction platform on the Mex67-Mtr2 receptor creates an overlap between mRNA and ribosome export. EMBO J. 2008;27:6-16 pubmed
    ..Thus, the Mex67-Mtr2 export receptor employs a versatile binding platform on its surface that could create a crosstalk between mRNA and ribosome export pathways. ..
  23. Matsuo Y, Granneman S, Thoms M, Manikas R, Tollervey D, Hurt E. Coupled GTPase and remodelling ATPase activities form a checkpoint for ribosome export. Nature. 2014;505:112-116 pubmed publisher
    ..We conclude that Nug2 is a regulatory GTPase that monitors pre-60S maturation, with release from its placeholder site linked to recruitment of the nuclear export machinery. ..
  24. Kappel L, Loibl M, Zisser G, Klein I, Fruhmann G, Gruber C, et al. Rlp24 activates the AAA-ATPase Drg1 to initiate cytoplasmic pre-60S maturation. J Cell Biol. 2012;199:771-82 pubmed publisher
    ..Our results show that release of Rlp24 by Drg1 defines a key event in large subunit formation that is a prerequisite for progression of cytoplasmic pre-60S maturation. ..
  25. Sarkar A, Pech M, Thoms M, Beckmann R, Hurt E. Ribosome-stalk biogenesis is coupled with recruitment of nuclear-export factor to the nascent 60S subunit. Nat Struct Mol Biol. 2016;23:1074-1082 pubmed publisher
    ..Thus, a spatiotemporal mark on the ribosomal stalk controls the recruitment of an RNA-export receptor to the nascent 60S subunit. ..
  26. Ting Y, Lu T, Johnson A, Shie J, Chen B, Kumar S S, et al. Bcp1 Is the Nuclear Chaperone of Rpl23 in Saccharomyces cerevisiae. J Biol Chem. 2017;292:585-596 pubmed publisher
    ..Loss of Bcp1 causes instability of Rpl23 and deficiency of 60S subunits. In summary, Bcp1 is a novel 60S biogenesis factor via chaperoning Rpl23 in the nucleus. ..
  27. Lee I, Li Z, Marcotte E. An improved, bias-reduced probabilistic functional gene network of baker's yeast, Saccharomyces cerevisiae. PLoS ONE. 2007;2:e988 pubmed
    ..YeastNet is available from http://www.yeastnet.org. ..