Gene Symbol: Nip7p
Description: ribosome biosynthesis protein NIP7
Alias: ribosome biosynthesis protein NIP7
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Miles T, Jakovljevic J, Horsey E, Harnpicharnchai P, Tang L, Woolford J. Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes. Mol Cell Biol. 2005;25:10419-32 pubmed
    ..Mutations in the WD40 motifs of Ytm1 disrupt binding to Erb1, destabilize the heterotrimer, and delay pre-rRNA processing and nuclear export of preribosomes. Nevertheless, 66S preribosomes lacking Ytm1 remain otherwise intact. ..
  2. Granato D, Gonzales F, Luz J, Cassiola F, Machado Santelli G, Oliveira C. Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae. FEBS J. 2005;272:4450-63 pubmed
    ..Nop53p also interacts with a second nucleolar protein, Nip7p. A carbon source-conditional strain with the NOP53 coding sequence under the control of the GAL1 promoter did not ..
  3. Venema J, Tollervey D. Ribosome synthesis in Saccharomyces cerevisiae. Annu Rev Genet. 1999;33:261-311 pubmed
    ..Here, we will discuss the recent, and often surprising, advances in our understanding of ribosome synthesis in the yeast Saccharomyces cerevisiae. These will underscore the unexpected complexity of eukaryotic ribosome synthesis. ..
  4. Bassler J, Grandi P, Gadal O, Lessmann T, Petfalski E, Tollervey D, et al. Identification of a 60S preribosomal particle that is closely linked to nuclear export. Mol Cell. 2001;8:517-29 pubmed
    ..Eight of these proteins were copurified with nuclear pore complexes, suggesting that this complex represents the transport intermediate for 60S subunit export. ..
  5. Zanchin N, Roberts P, DeSilva A, Sherman F, Goldfarb D. Saccharomyces cerevisiae Nip7p is required for efficient 60S ribosome subunit biogenesis. Mol Cell Biol. 1997;17:5001-15 pubmed
    ..products of open reading frames from humans, Caenorhabditis elegans, and Arabidopsis thaliana, indicating that Nip7p function is evolutionarily conserved. Gene disruption analysis demonstrated that NIP7 is essential for growth...
  6. Luz J, Georg R, Gomes C, Machado Santelli G, Oliveira C. Sdo1p, the yeast orthologue of Shwachman-Bodian-Diamond syndrome protein, binds RNA and interacts with nuclear rRNA-processing factors. Yeast. 2009;26:287-98 pubmed publisher
    ..Sdo1p binds RNA directly, suggesting that it may associate with the ribosomal subunits also through RNA interaction. ..
  7. Zanchin N, Goldfarb D. Nip7p interacts with Nop8p, an essential nucleolar protein required for 60S ribosome biogenesis, and the exosome subunit Rrp43p. Mol Cell Biol. 1999;19:1518-25 pubmed
    ..17:5001-5015, 1997). Rrp43p and a second essential protein, Nop8p, were identified in a two-hybrid screen as Nip7p-interacting proteins...
  8. Coltri P, Guimar es B, Granato D, Luz J, Teixeira E, Oliveira C, et al. Structural insights into the interaction of the Nip7 PUA domain with polyuridine RNA. Biochemistry. 2007;46:14177-87 pubmed publisher
    ..Yeast Nip7p interacts with nucleolar proteins and with the exosome subunit Rrp43p, but its molecular function remains to be ..
  9. Harnpicharnchai P, Jakovljevic J, Horsey E, Miles T, Roman J, Rout M, et al. Composition and functional characterization of yeast 66S ribosome assembly intermediates. Mol Cell. 2001;8:505-15 pubmed
    ..These results demonstrate that proteomic approaches in concert with genetic tools provide powerful means to purify and characterize ribosome assembly intermediates. ..

More Information


  1. Fuentes J, Datta K, Sullivan S, Walker A, Maddock J. In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1. Mol Genet Genomics. 2007;278:105-23 pubmed
    ..Several conserved charge-to-alanine substitutions outside the GTPase domain display modest growth phenotypes indicating that these residues are not critical for function. ..
  2. Goldfeder M, Oliveira C. Utp25p, a nucleolar Saccharomyces cerevisiae protein, interacts with U3 snoRNP subunits and affects processing of the 35S pre-rRNA. FEBS J. 2010;277:2838-52 pubmed publisher
    ..Our results indicate that Utp25p is a novel SSU processome subunit involved in pre-40S maturation. ..
  3. Santos M, Goldfeder M, Zanchin N, Oliveira C. The essential nucleolar yeast protein Nop8p controls the exosome function during 60S ribosomal subunit maturation. PLoS ONE. 2011;6:e21686 pubmed publisher
    The yeast nucleolar protein Nop8p has previously been shown to interact with Nip7p and to be required for 60S ribosomal subunit formation...
  4. Talkish J, Zhang J, Jakovljevic J, Horsey E, Woolford J. Hierarchical recruitment into nascent ribosomes of assembly factors required for 27SB pre-rRNA processing in Saccharomyces cerevisiae. Nucleic Acids Res. 2012;40:8646-61 pubmed
    ..Inhibition of TOR kinase decreases association of Rpf2, Spb4, Nog1 and Nog2 with pre-ribosomes. ..
  5. Goldfeder M, Oliveira C. Cwc24p, a novel Saccharomyces cerevisiae nuclear ring finger protein, affects pre-snoRNA U3 splicing. J Biol Chem. 2008;283:2644-53 pubmed
    ..These results led us to the conclusion that Cwc24p is involved in pre-U3 snoRNA splicing, indirectly affecting pre-rRNA processing. ..