Gene Symbol: NHA1
Description: Nha1p
Alias: Nha1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Ohgaki R, Nakamura N, Mitsui K, Kanazawa H. Characterization of the ion transport activity of the budding yeast Na+/H+ antiporter, Nha1p, using isolated secretory vesicles. Biochim Biophys Acta. 2005;1712:185-96 pubmed
    The Saccharomyces cerevisiae Nha1p, a plasma membrane protein belonging to the monovalent cation/proton antiporter family, plays a key role in the salt tolerance and pH regulation of cells...
  2. Bañuelos M, Sychrova H, Bleykasten Grosshans C, Souciet J, Potier S. The Nha1 antiporter of Saccharomyces cerevisiae mediates sodium and potassium efflux. Microbiology. 1998;144 ( Pt 10):2749-58 pubmed
    ..In addition, high K+ and Na+ efflux observed upon alkalinization of the cytoplasm implies a role of Nha1p in the regulation of intracellular pH...
  3. Proft M, Struhl K. MAP kinase-mediated stress relief that precedes and regulates the timing of transcriptional induction. Cell. 2004;118:351-61 pubmed
    ..stress activates Hog1 MAP kinase, which phosphorylates at least two proteins located at the plasma membrane, the Nha1 Na+/H+ antiporter and the Tok1 potassium channel...
  4. Kinclova Zimmermannova O, Gaskova D, Sychrova H. The Na+,K+/H+ -antiporter Nha1 influences the plasma membrane potential of Saccharomyces cerevisiae. FEMS Yeast Res. 2006;6:792-800 pubmed
    There are three different sodium transport systems (Ena1-4p, Nha1p, Nhx1p) in Saccharomyces cerevisiae. The effect of their absence on the tolerance to alkali-metal cations and on the membrane potential was studied...
  5. Zahradka J, Van Heusden G, Sychrova H. Yeast 14-3-3 proteins participate in the regulation of cell cation homeostasis via interaction with Nha1 alkali-metal-cation/proton antiporter. Biochim Biophys Acta. 2012;1820:849-58 pubmed publisher
    ..Instead, a genetic interaction was identified between BMH1 and NHA1 which encodes an Na+(K+)/H+ antiporter...
  6. Kinclova Zimmermannova O, Sychrova H. Functional study of the Nha1p C-terminus: involvement in cell response to changes in external osmolarity. Curr Genet. 2006;49:229-36 pubmed
    ..Upon sorbitol-mediated stress, the Nha1p potassium export activity decreases in order to maintain a higher intracellular concentration of solutes...
  7. Brett C, Tukaye D, Mukherjee S, Rao R. The yeast endosomal Na+K+/H+ exchanger Nhx1 regulates cellular pH to control vesicle trafficking. Mol Biol Cell. 2005;16:1396-405 pubmed
    ..This presents the newly defined family of eukaryotic endosomal NHE as novel targets for pharmacological inhibition to alleviate pathological states associated with organellar alkalinization. ..
  8. Petrezselyova S, Kinclova Zimmermannova O, Sychrova H. Vhc1, a novel transporter belonging to the family of electroneutral cation-Cl(-) cotransporters, participates in the regulation of cation content and morphology of Saccharomyces cerevisiae vacuoles. Biochim Biophys Acta. 2013;1828:623-31 pubmed publisher
    ..Considering its identified function, we propose to refer to the yeast YBR235w gene as VHC1 (vacuolar protein homologous to CCC family 1). ..
  9. Navarre C, Goffeau A. Membrane hyperpolarization and salt sensitivity induced by deletion of PMP3, a highly conserved small protein of yeast plasma membrane. EMBO J. 2000;19:2515-24 pubmed
    ..of PMP3 exacerbates the NaCl sensitivity phenotype of a mutant strain lacking the Pmr2p/Enap Na(+)-ATPases and the Nha1p Na(+)/H(+) antiporter, and suppresses the potassium dependency of a strain lacking the K(+) transporters, Trk1p ..

More Information


  1. Cagnac O, Aranda Sicilia M, Leterrier M, Rodriguez Rosales M, Venema K. Vacuolar cation/H+ antiporters of Saccharomyces cerevisiae. J Biol Chem. 2010;285:33914-22 pubmed publisher
    ..The relative importance of Nhx1p, Vnx1p, and Vcx1p in the vacuolar accumulation of monovalent cations will be discussed. ..
  2. Mitsui K, Yasui H, Nakamura N, Kanazawa H. Oligomerization of the Saccharomyces cerevisiae Na+/H+ antiporter Nha1p: implications for its antiporter activity. Biochim Biophys Acta. 2005;1720:125-36 pubmed
    The Na(+)/H(+) antiporter (Nha1p) from the budding yeast Saccharomyces cerevisiae plays an important role in intracellular pH and Na(+) homeostasis...
  3. Prior C, Potier S, Souciet J, Sychrova H. Characterization of the NHA1 gene encoding a Na+/H+-antiporter of the yeast Saccharomyces cerevisiae. FEBS Lett. 1996;387:89-93 pubmed
    The NHA1 gene (2958 nt) encoding a putative Na(+)/H(+) antiporter (986 aa) in Saccharomyces cerevisiae was cloned by selection based on increased NaCl tolerance...
  4. Mitsui K, Hatakeyama K, Matsushita M, Kanazawa H. Saccharomyces cerevisiae Na+/H+ antiporter Nha1p associates with lipid rafts and requires sphingolipid for stable localization to the plasma membrane. J Biochem. 2009;145:709-20 pubmed publisher
    The plasma membrane-type Na+/H+ antiporter Nha1p from budding yeast plays an important role in intracellular Na+ and pH homeostasis by mediating the exchange of Na+ for H+ across the plasma membrane...
  5. Hoeberichts F, Perez Valle J, Montesinos C, Mulet J, Planes M, Hueso G, et al. The role of K(+) and H(+) transport systems during glucose- and H(2)O(2)-induced cell death in Saccharomyces cerevisiae. Yeast. 2010;27:713-25 pubmed publisher
    ..A triple mutant ena1-4 tok1 nha1, devoid of K(+) efflux systems, is more tolerant to both glucose- and H(2)O(2)-induced cell death...
  6. Mitsui K, Kamauchi S, Nakamura N, Inoue H, Kanazawa H. A conserved domain in the tail region of the Saccharomyces cerevisiae Na+/H+ antiporter (Nha1p) plays important roles in localization and salinity-resistant cell-growth. J Biochem. 2004;135:139-48 pubmed
    The Saccharomyces cerevisiae Na(+)/H(+) antiporter Nha1p has a two-domain structure consisting of an N-terminal integral membrane region and a C-terminal cytoplasmic region...
  7. Pribylova L, Papouskova K, Zavrel M, Souciet J, Sychrova H. Exploration of yeast alkali metal cation/H+ antiporters: sequence and structure comparison. Folia Microbiol (Praha). 2006;51:413-24 pubmed
    The Saccharomyces cerevisiae genome contains three genes encoding alkali metal cation/H+ antiporters (Nha1p, Nhx1p, Kha1p) that differ in cell localization, substrate specificity and physiological function...
  8. Sant Ana G, Paes L, Paiva A, Fietto L, Totola A, Trópia M, et al. Protective effect of ions against cell death induced by acid stress in Saccharomyces. FEMS Yeast Res. 2009;9:701-12 pubmed publisher
    ..Additionally, the absence or low expression/activity of Ena proteins seems to be closely related to the basal membrane potential of the cells...
  9. Rosas Santiago P, Lagunas Gomez D, Yáñez Domínguez C, Vera Estrella R, Zimmermannova O, Sychrova H, et al. Plant and yeast cornichon possess a conserved acidic motif required for correct targeting of plasma membrane cargos. Biochim Biophys Acta Mol Cell Res. 2017;1864:1809-1818 pubmed publisher
    ..of these cargo receptors, however it modified the proper targeting of the plasma membrane transporters Nha1p, Pdr12p and Qdr2p...
  10. Merchan S, Pedelini L, Hueso G, Calzada A, Serrano R, Yenush L. Genetic alterations leading to increases in internal potassium concentrations are detrimental for DNA integrity in Saccharomyces cerevisiae. Genes Cells. 2011;16:152-65 pubmed publisher
  11. Maresova L, Sychrova H. Genetic interactions among the Arl1 GTPase and intracellular Na(+) /H(+) antiporters in pH homeostasis and cation detoxification. FEMS Yeast Res. 2010;10:802-11 pubmed publisher
    ..We conclude that Arl1p, Kha1p and Nhx1p collaborate in survival strategies at nonoptimal pH, temperatures and cation concentrations, but work independent of each other. ..
  12. Maresova L, Sychrova H. Physiological characterization of Saccharomyces cerevisiae kha1 deletion mutants. Mol Microbiol. 2005;55:588-600 pubmed
    ..One of the transporters formerly believed to extrude K+ from the yeast cells (besides Ena1-4p and Nha1p) was named Kha1p and presumed as a putative plasma membrane K+/H+ antiporter...
  13. Simon E, Barceló A, Arino J. Mutagenesis analysis of the yeast Nha1 Na+/H+ antiporter carboxy-terminal tail reveals residues required for function in cell cycle. FEBS Lett. 2003;545:239-45 pubmed
    The yeast Nha1 Na(+),K(+)/H(+) antiporter may play an important role in regulation of cell cycle, as high-copy expression of the NHA1 gene is able to rescue the blockage at the G(1)/S transition of cells lacking Sit4 protein phosphatase ..
  14. Snowdon C, Schierholtz R, Poliszczuk P, Hughes S, van der Merwe G. ETP1/YHL010c is a novel gene needed for the adaptation of Saccharomyces cerevisiae to ethanol. FEMS Yeast Res. 2009;9:372-80 pubmed publisher
    ..In addition, the hypersensitivity of etp1Delta to ethanol stress is partly due to the inability of the mutant to control the level of the cation/H(+) antiporter Nha1p in the cell.
  15. Simon E, Clotet J, Calero F, Ramos J, Arino J. A screening for high copy suppressors of the sit4 hal3 synthetically lethal phenotype reveals a role for the yeast Nha1 antiporter in cell cycle regulation. J Biol Chem. 2001;276:29740-7 pubmed
    A screening for multicopy suppressors of the G(1)/S blockage of a conditional sit4 hal3 mutant yielded the NHA1 gene, encoding a Na(+),K(+)/H(+) antiporter, composed of a transmembrane domain and a large carboxyl-terminal tail, which has ..
  16. Munoz I, Simon E, Casals N, Clotet J, Arino J. Identification of multicopy suppressors of cell cycle arrest at the G1-S transition in Saccharomyces cerevisiae. Yeast. 2003;20:157-69 pubmed
    ..The possible relationship between the known functions of these suppressor genes and the progress through the G(1)-S transition is discussed. ..
  17. Rosas Santiago P, Zimmermannova O, Vera Estrella R, Sychrová H, Pantoja O. Erv14 cargo receptor participates in yeast salt tolerance via its interaction with the plasma-membrane Nha1 cation/proton antiporter. Biochim Biophys Acta. 2016;1858:67-74 pubmed publisher
    The yeast Nha1p Na(+), K(+)/H(+) antiporter has a house-keeping role in pH and cation homeostasis. It is also needed to alleviate excess Na(+) or K(+) from the cytoplasm under high external concentrations of these cations...
  18. Mitsui K, Ochi F, Nakamura N, Doi Y, Inoue H, Kanazawa H. A novel membrane protein capable of binding the Na+/H+ antiporter (Nha1p) enhances the salinity-resistant cell growth of Saccharomyces cerevisiae. J Biol Chem. 2004;279:12438-47 pubmed
    The Na+/H+ antiporter Nha1p of Saccharomyces cerevisiae plays an important role in maintaining intracellular pH and Na+ homeostasis. Nha1p has a two-domain structure composed of integral membrane and hydrophilic tail regions...
  19. Zahradka J, Sychrova H. Plasma-membrane hyperpolarization diminishes the cation efflux via Nha1 antiporter and Ena ATPase under potassium-limiting conditions. FEMS Yeast Res. 2012;12:439-46 pubmed publisher
    ..various combinations of potassium efflux and uptake systems revealed that all three potassium-exporting systems the Nha1 antiporter, Ena ATPase and Tok1 channel contribute to potassium homeostasis and are active upon potassium ..