Gene Symbol: NEO1
Description: putative aminophospholipid-translocating P4-type ATPase NEO1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Wicky S, Schwarz H, Singer Krüger B. Molecular interactions of yeast Neo1p, an essential member of the Drs2 family of aminophospholipid translocases, and its role in membrane trafficking within the endomembrane system. Mol Cell Biol. 2004;24:7402-18 pubmed
    ..In agreement with that finding, the temperature-sensitive neo1-37 and neo1-69 mutants exhibit defects in receptor-mediated endocytosis, vacuole biogenesis, and vacuolar protein ..
  2. Hua Z, Graham T. Requirement for neo1p in retrograde transport from the Golgi complex to the endoplasmic reticulum. Mol Biol Cell. 2003;14:4971-83 pubmed
    ..Using conditional alleles of NEO1, we find that loss of Neo1p function causes cargo-specific defects in anterograde protein transport early in the ..
  3. Takeda M, Yamagami K, Tanaka K. Role of phosphatidylserine in phospholipid flippase-mediated vesicle transport in Saccharomyces cerevisiae. Eukaryot Cell. 2014;13:363-75 pubmed publisher
    ..These results suggest that flippase-dependent vesicle formation is mediated by phospholipid flipping, not by flipped phospholipids. ..
  4. Hua Z, Fatheddin P, Graham T. An essential subfamily of Drs2p-related P-type ATPases is required for protein trafficking between Golgi complex and endosomal/vacuolar system. Mol Biol Cell. 2002;13:3162-77 pubmed
    ..genome contains five genes encoding P-type ATPases that are potential aminophospholipid translocases (APTs): DRS2, NEO1, and three uncharacterized open reading frames that we have named DNF1, DNF2, and DNF3 for DRS2/NEO1 family...
  5. Saito K, Fujimura Kamada K, Furuta N, Kato U, Umeda M, Tanaka K. Cdc50p, a protein required for polarized growth, associates with the Drs2p P-type ATPase implicated in phospholipid translocation in Saccharomyces cerevisiae. Mol Biol Cell. 2004;15:3418-32 pubmed
    ..Thus, phospholipid asymmetry plays an important role in the establishment of cell polarity; the Cdc50p/Lem3p family likely constitute potential subunits specific to unique P-type ATPases of the APT subfamily...
  6. Yamagami K, Yamamoto T, Sakai S, Mioka T, Sano T, Igarashi Y, et al. Inositol depletion restores vesicle transport in yeast phospholipid flippase mutants. PLoS ONE. 2015;10:e0120108 pubmed publisher
    ..These results suggest that inositol depletion changes the lipid composition of the endosomal/TGN membranes, which results in vesicle formation from these membranes in the absence of flippases. ..
  7. Manlandro C, Palanivel V, Schorr E, Mihatov N, Antony A, Rosenwald A. Mon2 is a negative regulator of the monomeric G protein, Arl1. FEMS Yeast Res. 2012;12:637-50 pubmed publisher
    ..Our study expands understanding of the roles of Arl1 in vivo. ..
  8. Takar M, Wu Y, Graham T. The Essential Neo1 Protein from Budding Yeast Plays a Role in Establishing Aminophospholipid Asymmetry of the Plasma Membrane. J Biol Chem. 2016;291:15727-39 pubmed publisher
    ..Saccharomyces cerevisiae, for example, expresses five P4-ATPases, including Neo1, Drs2, Dnf1, Dnf2, and Dnf3...
  9. Wu Y, Takar M, Cuentas Condori A, Graham T. Neo1 and phosphatidylethanolamine contribute to vacuole membrane fusion in Saccharomyces cerevisiae. Cell Logist. 2016;6:e1228791 pubmed
    i>NEO1 is an essential gene in budding yeast and belongs to a highly conserved subfamily of P-type ATPase genes that encode phospholipid flippases...

More Information


  1. Singer Krüger B, Lasić M, Bürger A, Hausser A, Pipkorn R, Wang Y. Yeast and human Ysl2p/hMon2 interact with Gga adaptors and mediate their subcellular distribution. EMBO J. 2008;27:1423-35 pubmed publisher
    ..Deletion of either YSL2 or ARL1 causes mislocalization of Gga2p, whereas a neo1-69 mutant accumulates Gga2p on aberrant structures...
  2. Barbosa S, Pratte D, Schwarz H, Pipkorn R, Singer Krüger B. Oligomeric Dop1p is part of the endosomal Neo1p-Ysl2p-Arl1p membrane remodeling complex. Traffic. 2010;11:1092-106 pubmed publisher
    ..dop1-3 cells accumulate multivesicular, elongated tubular and ring-like structures similar to those displayed by neo1 and ysl2 mutants...
  3. Catty P, de Kerchove d Exaerde A, Goffeau A. The complete inventory of the yeast Saccharomyces cerevisiae P-type transport ATPases. FEBS Lett. 1997;409:325-32 pubmed
    ..It is proposed that the latter family which has homologs in Tetrahymena thermophila, Plasmodium falciparum and Caenorhabditis elegans constitutes a new group called P4-ATPases with characteristic topology and aminoacid signatures. ..
  4. Brett C, Kallay L, Hua Z, Green R, Chyou A, Zhang Y, et al. Genome-wide analysis reveals the vacuolar pH-stat of Saccharomyces cerevisiae. PLoS ONE. 2011;6:e17619 pubmed publisher
    ..An independent genetic screen revealed that correction of pH(v) dysregulation in a neo1(ts) mutant restored viability whereas cholesterol accumulation in human NPC1(-/-) fibroblasts diminished upon ..
  5. Yamamoto T, Fujimura Kamada K, Shioji E, Suzuki R, Tanaka K. Cfs1p, a Novel Membrane Protein in the PQ-Loop Family, Is Involved in Phospholipid Flippase Functions in Yeast. G3 (Bethesda). 2017;7:179-192 pubmed publisher
    ..Cfs1p may thus be a novel regulator of phospholipid asymmetry. ..