Gene Symbol: NDI1
Description: NADH-ubiquinone reductase (H(+)-translocating) NDI1
Alias: NADH-ubiquinone reductase (H(+)-translocating) NDI1
Species: Saccharomyces cerevisiae S288c
Products:     NDI1

Top Publications

  1. Bahadorani S, Cho J, Lo T, Contreras H, Lawal H, Krantz D, et al. Neuronal expression of a single-subunit yeast NADH-ubiquinone oxidoreductase (Ndi1) extends Drosophila lifespan. Aging Cell. 2010;9:191-202 pubmed publisher
    ..of the fruit fly Drosophila melanogaster with the alternative single-subunit NADH-ubiquinone oxidoreductase (Ndi1) of the baker's yeast Saccharomyces cerevisiae...
  2. de Vries S, Grivell L. Purification and characterization of a rotenone-insensitive NADH:Q6 oxidoreductase from mitochondria of Saccharomyces cerevisiae. Eur J Biochem. 1988;176:377-84 pubmed
    ..The relation of this enzyme with respect to various other NADH dehydrogenases from yeast and plant mitochondria is discussed. ..
  3. Marres C, de Vries S, Grivell L. Isolation and inactivation of the nuclear gene encoding the rotenone-insensitive internal NADH: ubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae. Eur J Biochem. 1991;195:857-62 pubmed
    ..This phenotype is discussed in terms of the interplay between different metabolic pathways in yeast. ..
  4. Li W, Sun L, Liang Q, Wang J, Mo W, Zhou B. Yeast AMID homologue Ndi1p displays respiration-restricted apoptotic activity and is involved in chronological aging. Mol Biol Cell. 2006;17:1802-11 pubmed
    ..Here, we show that overexpression of yeast AMID homologue internal NADH dehydrogenase (NDI1), but not external NADH dehydrogenase (NDE1), can cause apoptosis-like cell death, and this effect can be repressed ..
  5. Yamashita T, Nakamaru Ogiso E, Miyoshi H, Matsuno Yagi A, Yagi T. Roles of bound quinone in the single subunit NADH-quinone oxidoreductase (Ndi1) from Saccharomyces cerevisiae. J Biol Chem. 2007;282:6012-20 pubmed
    ..the biochemical basis for the function of the rotenone-insensitive internal NADH-quinone (Q) oxidoreductase (Ndi1), we have overexpressed mature Ndi1 in Escherichia coli membranes...
  6. Sanz A, Soikkeli M, Portero Otin M, Wilson A, Kemppainen E, McIlroy G, et al. Expression of the yeast NADH dehydrogenase Ndi1 in Drosophila confers increased lifespan independently of dietary restriction. Proc Natl Acad Sci U S A. 2010;107:9105-10 pubmed publisher
    ..In yeast, the single-subunit NADH dehydrogenase Ndi1 serves as a non-proton-translocating alternative enzyme that replaces complex I, bringing about the reoxidation of ..
  7. Seo B, Marella M, Yagi T, Matsuno Yagi A. The single subunit NADH dehydrogenase reduces generation of reactive oxygen species from complex I. FEBS Lett. 2006;580:6105-8 pubmed
    Using rat dopaminergic and human neuroblastoma cell lines transduced with the NDI1 gene encoding the internal NADH dehydrogenase (Ndi1) from Saccharomyces cerevisiae, we investigated reactive oxygen species (ROS) generation caused by ..
  8. Gomes F, Tahara E, Busso C, Kowaltowski A, Barros M. nde1 deletion improves mitochondrial DNA maintenance in Saccharomyces cerevisiae coenzyme Q mutants. Biochem J. 2013;449:595-603 pubmed publisher
    ..Altogether, our results indicate that impairment in the oxidation of cytosolic NADH by Nde1p is deleterious towards mitochondrial biogenesis due to an increase in reactive oxygen species release. ..
  9. Iwata M, Lee Y, Yamashita T, Yagi T, Iwata S, Cameron A, et al. The structure of the yeast NADH dehydrogenase (Ndi1) reveals overlapping binding sites for water- and lipid-soluble substrates. Proc Natl Acad Sci U S A. 2012;109:15247-52 pubmed
    ..The Ndi1 protein from Saccharomyces cerevisiae is a monotopic membrane protein, directed to the matrix...

More Information


  1. Zhao H, Wang Q, Liu C, Shang Y, Wen F, Wang F, et al. A Role for the Respiratory Chain in Regulating Meiosis Initiation in Saccharomyces cerevisiae. Genetics. 2018;208:1181-1194 pubmed publisher
    ..In summary, our studies unveiled the close relationship between mitochondria and sporulation, and uncover a novel meiosis initiation pathway that is regulated by the respiratory chain. ..
  2. Park J, Li Y, Bai Y. Yeast NDI1 improves oxidative phosphorylation capacity and increases protection against oxidative stress and cell death in cells carrying a Leber's hereditary optic neuropathy mutation. Biochim Biophys Acta. 2007;1772:533-42 pubmed
    ..The NDI1 gene, which encodes the internal NADH-quinone oxidoreductase in Saccharomyces cerevisiae, was introduced into the ..
  3. Matus Ortega M, Cárdenas Monroy C, Flores Herrera O, Mendoza Hernández G, Miranda M, González Pedrajo B, et al. New complexes containing the internal alternative NADH dehydrogenase (Ndi1) in mitochondria of Saccharomyces cerevisiae. Yeast. 2015;32:629-41 pubmed publisher
    ..three rotenone-insensitive NADH dehydrogenases distributed on both the external (Nde1 and Nde2) and internal (Ndi1) surfaces of the inner mitochondrial membrane...
  4. Grandier Vazeille X, Bathany K, Chaignepain S, Camougrand N, Manon S, Schmitter J. Yeast mitochondrial dehydrogenases are associated in a supramolecular complex. Biochemistry. 2001;40:9758-69 pubmed
    ..The association of these proteins is discussed in terms of NADH-channeling. ..
  5. Cui Y, Zhao S, Wu Z, Dai P, Zhou B. Mitochondrial release of the NADH dehydrogenase Ndi1 induces apoptosis in yeast. Mol Biol Cell. 2012;23:4373-82 pubmed publisher
    Saccharomyces cerevisiae NDI1 codes for the internal mitochondrial ubiquinone oxidoreductase, which transfers electrons from NADH to ubiquinone in the respiratory chain...
  6. Yang Y, Yamashita T, Nakamaru Ogiso E, Hashimoto T, Murai M, Igarashi J, et al. Reaction mechanism of single subunit NADH-ubiquinone oxidoreductase (Ndi1) from Saccharomyces cerevisiae: evidence for a ternary complex mechanism. J Biol Chem. 2011;286:9287-97 pubmed publisher
    The flavoprotein rotenone-insensitive internal NADH-ubiquinone (UQ) oxidoreductase (Ndi1) is a member of the respiratory chain in Saccharomyces cerevisiae...
  7. Perry C, Huang C, Liu W, Magee N, Carreira R, Gottlieb R. Xenotransplantation of mitochondrial electron transfer enzyme, Ndi1, in myocardial reperfusion injury. PLoS ONE. 2011;6:e16288 pubmed publisher
    ..The yeast, S. cerevisiae, expresses internal rotenone insensitive NADH-quinone oxidoreductase (Ndi1); a single 56 kDa polypeptide which, like the multi-subunit mammalian complex I, serves as the entry site of ..
  8. Murai M, Yamashita T, Senoh M, Mashimo Y, Kataoka M, Kosaka H, et al. Characterization of the ubiquinone binding site in the alternative NADH-quinone oxidoreductase of Saccharomyces cerevisiae by photoaffinity labeling. Biochemistry. 2010;49:2973-80 pubmed publisher
    The Ndi1 enzyme found in the mitochondrial membrane of Saccharomyces cerevisiae is an NDH-2-type alternative NADH-quinone oxidoreductase...
  9. Bakker B, Bro C, Kotter P, Luttik M, van Dijken J, Pronk J. The mitochondrial alcohol dehydrogenase Adh3p is involved in a redox shuttle in Saccharomyces cerevisiae. J Bacteriol. 2000;182:4730-7 pubmed
    b>NDI1 is the unique gene encoding the internal mitochondrial NADH dehydrogenase of Saccharomyces cerevisiae. The enzyme catalyzes the transfer of electrons from intramitochondrial NADH to ubiquinone...
  10. Feng Y, Li W, Li J, Wang J, Ge J, Xu D, et al. Structural insight into the type-II mitochondrial NADH dehydrogenases. Nature. 2012;491:478-82 pubmed publisher
    ..3) in catalysing electron transfer from NADH to ubiquinone in the mitochondrial respiratory chain. The yeast NDH-2 (Ndi1) oxidizes NADH on the matrix side and reduces ubiquinone to maintain mitochondrial NADH/NAD(+) homeostasis...
  11. Vilain S, Esposito G, Haddad D, Schaap O, Dobreva M, Vos M, et al. The yeast complex I equivalent NADH dehydrogenase rescues pink1 mutants. PLoS Genet. 2012;8:e1002456 pubmed publisher