NDC80

Summary

Gene Symbol: NDC80
Description: kinetochore-associated Ndc80 complex subunit NDC80
Alias: HEC1, TID3, kinetochore-associated Ndc80 complex subunit NDC80
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Ohkuni K, Abdulle R, Tong A, Boone C, Kitagawa K. Ybp2 associates with the central kinetochore of Saccharomyces cerevisiae and mediates proper mitotic progression. PLoS ONE. 2008;3:e1617 pubmed publisher
    ..physically associates with proteins of the COMA complex (Ctf19, Okp1, Mcm21, and Ame1) and 3 components of the Ndc80 complex (Ndc80, Nuf2, and Spc25 but not Spc24) in the central kinetochore and with Cse4 (the centromeric histone ..
  2. Zheng L, Chen Y, Lee W. Hec1p, an evolutionarily conserved coiled-coil protein, modulates chromosome segregation through interaction with SMC proteins. Mol Cell Biol. 1999;19:5417-28 pubmed
    ..Budding yeast cells deleted in the scHEC1/NDC80 allele are not viable, but this lethal phenotype can be rescued by hsHEC1 under control of the endogenous scHEC1 ..
  3. Kemmler S, Stach M, Knapp M, Ortiz J, Pfannstiel J, Ruppert T, et al. Mimicking Ndc80 phosphorylation triggers spindle assembly checkpoint signalling. EMBO J. 2009;28:1099-110 pubmed publisher
    ..We found that Saccharomyces cerevisiae Mps1 interacts physically with the N-terminal domain of Ndc80 (Ndc80(1-257)), a constituent of the Ndc80 kinetochore complex...
  4. Collins K, Castillo A, Tatsutani S, Biggins S. De novo kinetochore assembly requires the centromeric histone H3 variant. Mol Biol Cell. 2005;16:5649-60 pubmed
    ..Consistent with this observation, Cse4-depleted cells had severe chromosome segregation defects. We therefore propose that yeast kinetochore assembly requires both centromeric DNA specificity and centromeric chromatin. ..
  5. He X, Rines D, Espelin C, Sorger P. Molecular analysis of kinetochore-microtubule attachment in budding yeast. Cell. 2001;106:195-206 pubmed
    ..The proteins include both molecular motors and microtubule associated proteins (MAPs), implying that motors and MAPs function together in binding chromosomes to spindle microtubules. ..
  6. Maure J, Komoto S, Oku Y, Mino A, Pasqualato S, Natsume K, et al. The Ndc80 loop region facilitates formation of kinetochore attachment to the dynamic microtubule plus end. Curr Biol. 2011;21:207-13 pubmed publisher
    ..The mechanism for this conversion is still elusive. The Ndc80 complex is an essential component of the KT-MT interface, and here we studied a role of the Ndc80 loop region, a ..
  7. London N, Biggins S. Mad1 kinetochore recruitment by Mps1-mediated phosphorylation of Bub1 signals the spindle checkpoint. Genes Dev. 2014;28:140-52 pubmed publisher
    ..The Mad1 interaction with Bub1 and kinetochores can be reconstituted in the presence of Mps1 and Mad2. Together, this work reveals a critical mechanism that determines kinetochore activation of the spindle checkpoint. ..
  8. Akiyoshi B, Nelson C, Ranish J, Biggins S. Analysis of Ipl1-mediated phosphorylation of the Ndc80 kinetochore protein in Saccharomyces cerevisiae. Genetics. 2009;183:1591-5 pubmed publisher
    Phosphorylation of the Ndc80 kinetochore protein by the Ipl1/Aurora B kinase reduces its microtubule binding activity in vitro...
  9. London N, Ceto S, Ranish J, Biggins S. Phosphoregulation of Spc105 by Mps1 and PP1 regulates Bub1 localization to kinetochores. Curr Biol. 2012;22:900-6 pubmed publisher
    ..Together, these data identify Spc105 as a key target of the Mps1 kinase and show that the opposing activities of Mps1 and PP1 regulate the kinetochore localization of the Bub1 protein...

More Information

Publications52

  1. Bock L, Pagliuca C, Kobayashi N, Grove R, Oku Y, Shrestha K, et al. Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore. Nat Cell Biol. 2012;14:614-24 pubmed publisher
    ..binding and chromosome segregation are mediated by the multi-copy KNL1(Spc105), MIS12(Mtw1) and NDC80(Ndc80) complexes that form the so-called KMN network...
  2. Wigge P, Kilmartin J. The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol. 2001;152:349-60 pubmed
    ..Temperature-sensitive mutants in NDC80, SPC25, and SPC24 show defects in chromosome segregation...
  3. Janke C, Ortiz J, Lechner J, Shevchenko A, Magiera M, Schramm C, et al. The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control. EMBO J. 2001;20:777-91 pubmed
    ..Since Ndc80p, Nuf2p and Spc24p are conserved proteins, it is likely that similar complexes are part of the kinetochore in other organisms. ..
  4. Hyland K, Kingsbury J, Koshland D, Hieter P. Ctf19p: A novel kinetochore protein in Saccharomyces cerevisiae and a potential link between the kinetochore and mitotic spindle. J Cell Biol. 1999;145:15-28 pubmed
    ..We propose that Ctf19p is part of a macromolecular kinetochore complex, which may function as a link between the kinetochore and the mitotic spindle. ..
  5. Powers A, Franck A, Gestaut D, Cooper J, Gracyzk B, Wei R, et al. The Ndc80 kinetochore complex forms load-bearing attachments to dynamic microtubule tips via biased diffusion. Cell. 2009;136:865-75 pubmed publisher
    ..Genetic, biochemical, and structural studies implicate the Ndc80 complex as a direct point of contact between kinetochores and microtubules, but these approaches provide only a ..
  6. Hornung P, Maier M, Alushin G, Lander G, Nogales E, Westermann S. Molecular architecture and connectivity of the budding yeast Mtw1 kinetochore complex. J Mol Biol. 2011;405:548-59 pubmed publisher
    ..heterodimers consisting of Mtw1p-Nnf1p and Dsn1p-Nsl1p, and it interacts directly with the microtubule-binding Ndc80 kinetochore complex via the centromere-proximal Spc24/Spc25 head domain...
  7. Tien J, Umbreit N, Gestaut D, Franck A, Cooper J, Wordeman L, et al. Cooperation of the Dam1 and Ndc80 kinetochore complexes enhances microtubule coupling and is regulated by aurora B. J Cell Biol. 2010;189:713-23 pubmed publisher
    ..As microtubule-binding elements, the budding yeast Ndc80 and Dam1 kinetochore complexes are essential and not redundant, but their distinct contributions are unknown...
  8. Nekrasov V, Smith M, Peak Chew S, Kilmartin J. Interactions between centromere complexes in Saccharomyces cerevisiae. Mol Biol Cell. 2003;14:4931-46 pubmed
    ..These results show an increasingly complex structure for the S. cerevisiae centromere and a probable conservation of structure between parts of the centromeres of S. cerevisiae and S. pombe. ..
  9. Schmitzberger F, Harrison S. RWD domain: a recurring module in kinetochore architecture shown by a Ctf19-Mcm21 complex structure. EMBO Rep. 2012;13:216-22 pubmed publisher
    ..Like many eukaryotic molecular machines, kinetochores may have evolved from simpler assemblies by multiplication of a few ancestral modules. ..
  10. Wei R, Sorger P, Harrison S. Molecular organization of the Ndc80 complex, an essential kinetochore component. Proc Natl Acad Sci U S A. 2005;102:5363-7 pubmed
    The four-protein Ndc80 complex, an essential kinetochore component conserved from yeast to humans, plays an indispensable role in proper chromosome alignment and segregation during mitosis...
  11. Demirel P, Keyes B, Chaterjee M, Remington C, Burke D. A redundant function for the N-terminal tail of Ndc80 in kinetochore-microtubule interaction in Saccharomyces cerevisiae. Genetics. 2012;192:753-6 pubmed publisher
    The N-terminal tail of Ndc80 is essential for kinetochore-microtubule binding in human cells but is not required for viability in yeast...
  12. Janke C, Ortiz J, Tanaka T, Lechner J, Schiebel E. Four new subunits of the Dam1-Duo1 complex reveal novel functions in sister kinetochore biorientation. EMBO J. 2002;21:181-93 pubmed
    ..Monopolar attached sister centromeres are resolved to bipolar attachment in wild-type cells but persist in spc34-3 cells. ..
  13. Malvezzi F, Litos G, Schleiffer A, Heuck A, Mechtler K, Clausen T, et al. A structural basis for kinetochore recruitment of the Ndc80 complex via two distinct centromere receptors. EMBO J. 2013;32:409-23 pubmed publisher
    The Ndc80 complex is the key microtubule-binding element of the kinetochore...
  14. Akiyoshi B, Nelson C, Biggins S. The aurora B kinase promotes inner and outer kinetochore interactions in budding yeast. Genetics. 2013;194:785-9 pubmed publisher
    ..The Dsn1 component is crucial for kinetochore assembly and is phosphorylated by the Aurora B kinase. We found that Aurora B phosphorylation of Dsn1 promotes the interaction between outer and inner kinetochore proteins in budding yeast. ..
  15. Wang H, Long S, Ciferri C, Westermann S, Drubin D, Barnes G, et al. Architecture and flexibility of the yeast Ndc80 kinetochore complex. J Mol Biol. 2008;383:894-903 pubmed publisher
    ..The highly conserved Ndc80 kinetochore complex makes direct contacts with the microtubule and is essential for spindle checkpoint signaling...
  16. Shang C, Hazbun T, Cheeseman I, Aranda J, Fields S, Drubin D, et al. Kinetochore protein interactions and their regulation by the Aurora kinase Ipl1p. Mol Biol Cell. 2003;14:3342-55 pubmed
    ..Strikingly, we found that the Dam1p-Ndc80p and Dam1p-Spc34p interactions were weakened by mutations mimicking phosphorylation at Ipl1p sites, allowing us to formulate a model for the effects of phosphoregulation on kinetochore function. ..
  17. Schweiggert J, Stevermann L, Panigada D, Kammerer D, Liakopoulos D. Regulation of a Spindle Positioning Factor at Kinetochores by SUMO-Targeted Ubiquitin Ligases. Dev Cell. 2016;36:415-27 pubmed publisher
    ..We propose that kinetochores serve as platforms to recruit STUbLs in a SUMO-dependent manner in order to ensure correct spindle function by regulating levels of microtubule-associated proteins. ..
  18. Dagkessamanskaia A, Durand F, Uversky V, Binda M, Lopez F, El Azzouzi K, et al. Functional dissection of an intrinsically disordered protein: understanding the roles of different domains of Knr4 protein in protein-protein interactions. Protein Sci. 2010;19:1376-85 pubmed publisher
  19. Ortiz J, Funk C, Schäfer A, Lechner J. Stu1 inversely regulates kinetochore capture and spindle stability. Genes Dev. 2009;23:2778-91 pubmed publisher
    ..Furthermore, Stu1 localizes to kinetochores. Here we show that, in prometaphase, Stu1 assembles in an Ndc80-dependent manner exclusively at kinetochores that are not attached to microtubules...
  20. Liu C, van Dyk D, Choe V, Yan J, Majumder S, Costanzo M, et al. Ubiquitin ligase Ufd2 is required for efficient degradation of Mps1 kinase. J Biol Chem. 2011;286:43660-7 pubmed publisher
    ..We demonstrate that Ufd2-mediated Mps1 degradation is conserved in humans. Our results underscore the significance of Ufd2 in proteolysis and further suggest that Ufd2-like enzymes regulate far more substrates than previously envisioned...
  21. Chen Y, Riley D, Zheng L, Chen P, Lee W. Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation. J Biol Chem. 2002;277:49408-16 pubmed
    b>Hec1 (highly expressed in cancer) plays essential roles in chromosome segregation by interacting through its coiled-coil domains with several proteins that modulate the G(2)/M phase...
  22. Aravamudhan P, Felzer Kim I, Gurunathan K, Joglekar A. Assembling the protein architecture of the budding yeast kinetochore-microtubule attachment using FRET. Curr Biol. 2014;24:1437-46 pubmed publisher
    ..FRET and high-resolution colocalization measurements involving the essential MT-binding kinetochore components: Ndc80, Dam1, Spc105, and Stu2...
  23. Kalantzaki M, Kitamura E, Zhang T, Mino A, Novák B, Tanaka T. Kinetochore-microtubule error correction is driven by differentially regulated interaction modes. Nat Cell Biol. 2015;17:421-33 pubmed publisher
    ..how aurora B specifically promotes disruption of the end-on attachment through phospho-regulation of kinetochore components Dam1 and Ndc80. Our results reveal fundamental mechanisms for promoting error correction for bi-orientation.
  24. Meyer R, Chuong H, Hild M, Hansen C, Kinter M, Dawson D. Ipl1/Aurora-B is necessary for kinetochore restructuring in meiosis I in Saccharomyces cerevisiae. Mol Biol Cell. 2015;26:2986-3000 pubmed publisher
    ..The Ndc80 outer kinetochore complex, but not other subcomplexes, is shed upon meiotic entry...
  25. Lampert F, Mieck C, Alushin G, Nogales E, Westermann S. Molecular requirements for the formation of a kinetochore-microtubule interface by Dam1 and Ndc80 complexes. J Cell Biol. 2013;200:21-30 pubmed publisher
    ..kinetochore-microtubule interface is formed by the plus end-associated Dam1 complex and the kinetochore-resident Ndc80 complex, but how they work in combination and whether a physical association between them is critical for ..
  26. Aravamudhan P, Chen R, Roy B, Sim J, Joglekar A. Dual mechanisms regulate the recruitment of spindle assembly checkpoint proteins to the budding yeast kinetochore. Mol Biol Cell. 2016;27:3405-3417 pubmed
    ..These data reveal that kinetochore-intrinsic and -extrinsic mechanisms influence the physiological operation of SAC signaling, potentially to maximize chromosome segregation accuracy. ..
  27. Sarangapani K, Akiyoshi B, Duggan N, Biggins S, Asbury C. Phosphoregulation promotes release of kinetochores from dynamic microtubules via multiple mechanisms. Proc Natl Acad Sci U S A. 2013;110:7282-7 pubmed publisher
    ..The conserved kinase Aurora B phosphorylates the major microtubule-binding kinetochore subcomplexes, Ndc80 and (in yeast) Dam1, to promote release of erroneous attachments, giving another chance for proper attachments to ..
  28. Dresser M, Ewing D, Conrad M, Dominguez A, Barstead R, Jiang H, et al. DMC1 functions in a Saccharomyces cerevisiae meiotic pathway that is largely independent of the RAD51 pathway. Genetics. 1997;147:533-44 pubmed
    ..Interestingly, the pattern on CHEF gels of chromosome fragments that result from meiotic DNA double-strand break formation is different in DMC1 mutant strains from that seen in rad50S strains. ..
  29. Robinson L, Phillips J, Brou L, Boswell E, Tatchell K. Suppressors of ipl1-2 in components of a Glc7 phosphatase complex, Cdc48 AAA ATPase, TORC1, and the kinetochore. G3 (Bethesda). 2012;2:1687-701 pubmed publisher
    ..rev8 contains the missense mutation ndc80-K204E...
  30. Le Masson I, Saveanu C, Chevalier A, Namane A, Gobin R, Fromont Racine M, et al. Spc24 interacts with Mps2 and is required for chromosome segregation, but is not implicated in spindle pole body duplication. Mol Microbiol. 2002;43:1431-43 pubmed
    ..Previous two-hybrid screens with Spc24 as bait had identified Spc25 and Ndc80 as putative interacting partners, and we verified these interactions in vivo by purification of TAP-tagged ..
  31. Winsor B, Schiebel E. Review: an overview of the Saccharomyces cerevisiae microtubule and microfilament cytoskeleton. Yeast. 1997;13:399-434 pubmed
  32. Akiyoshi B, Nelson C, Duggan N, Ceto S, Ranish J, Biggins S. The Mub1/Ubr2 ubiquitin ligase complex regulates the conserved Dsn1 kinetochore protein. PLoS Genet. 2013;9:e1003216 pubmed publisher
    ..We propose that Mub1/Ubr2 are part of a quality control system that monitors kinetochore integrity, thus ensuring genomic stability. ..
  33. Makhnevych T, Wong P, Pogoutse O, Vizeacoumar F, Greenblatt J, Emili A, et al. Hsp110 is required for spindle length control. J Cell Biol. 2012;198:623-36 pubmed publisher
    ..These data suggest a model whereby the Hsp70-Hsp110 chaperone complex antagonizes Cin8 plus-end motility and prevents premature spindle elongation in S phase. ..
  34. Boeckmann L, Takahashi Y, Au W, Mishra P, Choy J, Dawson A, et al. Phosphorylation of centromeric histone H3 variant regulates chromosome segregation in Saccharomyces cerevisiae. Mol Biol Cell. 2013;24:2034-44 pubmed publisher
    ..cse4-4SD mutant suppresses the temperature-sensitive growth of ipl1-2 and Ipl1 substrate mutants dam1 spc34 and ndc80, which are defective for chromosome biorientation...
  35. Gandhi S, Gierlinski M, Mino A, Tanaka K, Kitamura E, Clayton L, et al. Kinetochore-dependent microtubule rescue ensures their efficient and sustained interactions in early mitosis. Dev Cell. 2011;21:920-33 pubmed publisher
    ..Thus, kinetochore-dependent microtubule rescue ensures efficient and sustained kinetochore-microtubule interactions in early mitosis. ..
  36. Araki Y, Gombos L, Migueleti S, Sivashanmugam L, Antony C, Schiebel E. N-terminal regions of Mps1 kinase determine functional bifurcation. J Cell Biol. 2010;189:41-56 pubmed publisher
    ..Mps1 phosphorylation of Cdc31 at the conserved T110 residue controls substrate binding to Kar1 protein. These findings explain the multiple SPB duplication defects of mps1 mutants on a molecular level. ..
  37. Hornung P, Troć P, Malvezzi F, Maier M, Demianova Z, Zimniak T, et al. A cooperative mechanism drives budding yeast kinetochore assembly downstream of CENP-A. J Cell Biol. 2014;206:509-24 pubmed publisher
    ..This two-step mechanism may protect against inappropriate kinetochore assembly similar to rate-limiting nucleation steps used by cytoskeletal polymers. ..
  38. Pagliuca C, Draviam V, Marco E, Sorger P, De Wulf P. Roles for the conserved spc105p/kre28p complex in kinetochore-microtubule binding and the spindle assembly checkpoint. PLoS ONE. 2009;4:e7640 pubmed publisher
    ..We find Spc105p/Kre28p to comprise the third linker complex that, along with the Ndc80 and MIND linker complexes, is responsible for bridging between centromeric heterochromatin and kinetochore MAPs ..
  39. Snead J, Sullivan M, Lowery D, Cohen M, Zhang C, Randle D, et al. A coupled chemical-genetic and bioinformatic approach to Polo-like kinase pathway exploration. Chem Biol. 2007;14:1261-72 pubmed
    ..Finally, an examination of Cdc5 binding by SPB-localized proteins expanded our knowledge of Cdc5 function at the SPB. ..
  40. Miller M, Asbury C, Biggins S. A TOG Protein Confers Tension Sensitivity to Kinetochore-Microtubule Attachments. Cell. 2016;165:1428-1439 pubmed publisher
    ..Stu2 and its human ortholog, ch-TOG, exhibit a conserved interaction with the Ndc80 kinetochore complex that strengthens its attachment to microtubule tips...
  41. Tien J, Fong K, Umbreit N, Payen C, Zelter A, Asbury C, et al. Coupling unbiased mutagenesis to high-throughput DNA sequencing uncovers functional domains in the Ndc80 kinetochore protein of Saccharomyces cerevisiae. Genetics. 2013;195:159-70 pubmed publisher
    ..This linkage depends on the Ndc80 complex, a conserved and essential microtubule-binding component of the kinetochore...
  42. Tien J, Umbreit N, Zelter A, Riffle M, Hoopmann M, Johnson R, et al. Kinetochore biorientation in Saccharomyces cerevisiae requires a tightly folded conformation of the Ndc80 complex. Genetics. 2014;198:1483-93 pubmed publisher
    ..Kinetochore-microtubule attachments require the essential Ndc80 complex, which contains two globular ends linked by large coiled-coil domains...
  43. Kim J, Zelter A, Umbreit N, Bollozos A, Riffle M, Johnson R, et al. The Ndc80 complex bridges two Dam1 complex rings. elife. 2017;6: pubmed publisher
    ..The Dam1 and Ndc80 complexes are the main microtubule binding components of the Saccharomyces cerevisiae kinetochore...