Gene Symbol: NCS2
Alias: TUC2, Ncs2p
Species: Saccharomyces cerevisiae S288c
Goehring A, Rivers D, Sprague G. Urmylation: a ubiquitin-like pathway that functions during invasive growth and budding in yeast. Mol Biol Cell. 2003;14:4329-41 pubmed
..Loss of five genes that are also essential in a cla4Delta strain, NCS2, NCS6, ELP2, ELP6, and URE2, affect the level of at least one Urm1p conjugate...
Esberg A, Huang B, Johansson M, Byström A. Elevated levels of two tRNA species bypass the requirement for elongator complex in transcription and exocytosis. Mol Cell. 2006;24:139-48 pubmed
..Our results indicate that the physiologically relevant function of the evolutionary-conserved Elongator complex is in formation of modified nucleosides in tRNAs. ..
Nakai Y, Nakai M, Hayashi H. Thio-modification of yeast cytosolic tRNA requires a ubiquitin-related system that resembles bacterial sulfur transfer systems. J Biol Chem. 2008;283:27469-76 pubmed publisher
..We found that ubiquitin-like protein Urm1 and ubiquitin-activating enzyme-like protein Uba4, as well as Tuc1 and Tuc2, were strictly required for the s(2) modification...
Huang B, Lu J, Byström A. A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. RNA. 2008;14:2183-94 pubmed publisher
..In addition to earlier described mutants, formation of the s(2) group was also abolished in urm1, uba4, and ncs2 mutants and decreased in the yor251c mutant...
Noma A, Sakaguchi Y, Suzuki T. Mechanistic characterization of the sulfur-relay system for eukaryotic 2-thiouridine biogenesis at tRNA wobble positions. Nucleic Acids Res. 2009;37:1335-52 pubmed publisher
..The activated thiocarboxylate can be utilized in the subsequent reactions for 2-thiouridine formation, mediated by Ncs2p/Ncs6p. We could successfully reconstitute the 2-thiouridine formation in vitro using recombinant proteins...
Chen C, Huang B, Eliasson M, Ryden P, Byström A. Elongator complex influences telomeric gene silencing and DNA damage response by its role in wobble uridine tRNA modification. PLoS Genet. 2011;7:e1002258 pubmed publisher
..Defects in telomeric gene silencing and DNA damage response are also observed in strains with the tuc2? mutation, which abolish the formation of the 2-thio group of the mcm?s²U nucleoside in tRNA(Lys)(mcm?s²UUU), ..
Lee M, Dohlman H. Coactivation of G protein signaling by cell-surface receptors and an intracellular exchange factor. Curr Biol. 2008;18:211-5 pubmed publisher
..Our data suggest that intracellular proteins function in cooperation with mating pheromones to amplify G protein signaling, thereby leading to full pathway activation. ..
Sinha H, David L, Pascon R, Clauder Münster S, Krishnakumar S, Nguyen M, et al
. Sequential elimination of major-effect contributors identifies additional quantitative trait loci conditioning high-temperature growth in yeast. Genetics. 2008;180:1661-70 pubmed publisher
..One QTL, which is linked to the previously identified major-effect QTL, was dissected, and NCS2 was identified as the causative gene...
Scheidt V, Jüdes A, Bär C, Klassen R, Schaffrath R. Loss of wobble uridine modification in tRNA anticodons interferes with TOR pathway signaling. Microb Cell. 2014;1:416-424 pubmed publisher
..i>ppm1?, rrd1?) and U34 modification defects (elp3?, kti12?, urm1?, ncs2?) and found the rapamycin hypersensitivity in the latter is epistatic to drug resistance of the former...