Gene Symbol: NBP35
Description: Fe-S cluster-binding ATPase
Alias: Fe-S cluster-binding ATPase
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Vitale G, Fabre E, Hurt E. NBP35 encodes an essential and evolutionary conserved protein in Saccharomyces cerevisiae with homology to a superfamily of bacterial ATPases. Gene. 1996;178:97-106 pubmed
    We have cloned a novel and essential gene, NBP35, from Saccharomyces cerevisiae that encodes a putative Nucleotide Binding Protein of 35 kDa...
  2. Netz D, Pierik A, Stümpfig M, Muhlenhoff U, Lill R. The Cfd1-Nbp35 complex acts as a scaffold for iron-sulfur protein assembly in the yeast cytosol. Nat Chem Biol. 2007;3:278-86 pubmed
    ..So far, four highly conserved proteins (Cfd1, Nbp35, Nar1 and Cia1) have been identified as members of the cytosolic [Fe-S] protein assembly machinery, but their ..
  3. Huang B, Lu J, Byström A. A genome-wide screen identifies genes required for formation of the wobble nucleoside 5-methoxycarbonylmethyl-2-thiouridine in Saccharomyces cerevisiae. RNA. 2008;14:2183-94 pubmed publisher
    ..Like the absence of the mcm(5) side chain, the lack of the s(2) group renders tRNA(mcm5s2UUC Glu) less sensitive to gamma-toxin, reinforcing the importance of the wobble nucleoside mcm(5)s(2)U for tRNA cleavage by gamma-toxin. ..
  4. Netz D, Pierik A, Stümpfig M, Bill E, Sharma A, Pallesen L, et al. A bridging [4Fe-4S] cluster and nucleotide binding are essential for function of the Cfd1-Nbp35 complex as a scaffold in iron-sulfur protein maturation. J Biol Chem. 2012;287:12365-78 pubmed publisher
    The essential P-loop NTPases Cfd1 and Nbp35 of the cytosolic iron-sulfur (Fe-S) protein assembly machinery perform a scaffold function for Fe-S cluster synthesis...
  5. Netz D, Stümpfig M, Dore C, Muhlenhoff U, Pierik A, Lill R. Tah18 transfers electrons to Dre2 in cytosolic iron-sulfur protein biogenesis. Nat Chem Biol. 2010;6:758-65 pubmed publisher
    ..The pathway is conserved in eukaryotes, as human Ndor1-Ciapin1 proteins can functionally replace yeast Tah18-Dre2. ..
  6. Chanet R, Heude M. Characterization of mutations that are synthetic lethal with pol3-13, a mutated allele of DNA polymerase delta in Saccharomyces cerevisiae. Curr Genet. 2003;43:337-50 pubmed
    ..Finally, mutations in three genes of unknown function were isolated (NBP35, DRE2, TAH18)...
  7. Yarunin A, Panse V, Petfalski E, Dez C, Tollervey D, Hurt E. Functional link between ribosome formation and biogenesis of iron-sulfur proteins. EMBO J. 2005;24:580-8 pubmed
    In genetic screens for ribosomal export mutants, we identified CFD1, NBP35 and NAR1 as factors involved in ribosome biogenesis...
  8. Pallesen L, Solodovnikova N, Sharma A, Walden W. Interaction with Cfd1 increases the kinetic lability of FeS on the Nbp35 scaffold. J Biol Chem. 2013;288:23358-67 pubmed publisher
    P-loop NTPases of the ApbC/Nbp35 family are involved in FeS protein maturation in nearly all organisms and are proposed to function as scaffolds for initial FeS cluster assembly...
  9. Hausmann A, Aguilar Netz D, Balk J, Pierik A, Muhlenhoff U, Lill R. The eukaryotic P loop NTPase Nbp35: an essential component of the cytosolic and nuclear iron-sulfur protein assembly machinery. Proc Natl Acad Sci U S A. 2005;102:3266-71 pubmed
    ..Here, we functionally characterized the first member of the Mrp/Nbp35 subbranch of this family, the essential Nbp35p of Saccharomyces cerevisiae...

More Information


  1. Balk J, Aguilar Netz D, Tepper K, Pierik A, Lill R. The essential WD40 protein Cia1 is involved in a late step of cytosolic and nuclear iron-sulfur protein assembly. Mol Cell Biol. 2005;25:10833-41 pubmed
    ..machineries in mitochondria and three recently identified extramitochondrial proteins, the P-loop NTPases Cfd1 and Nbp35 and the hydrogenase-like Nar1...
  2. Ueta R, Fujiwara N, Iwai K, Yamaguchi Iwai Y. Iron-induced dissociation of the Aft1p transcriptional regulator from target gene promoters is an initial event in iron-dependent gene suppression. Mol Cell Biol. 2012;32:4998-5008 pubmed publisher
  3. Camire E, Grossman J, Thole G, Fleischman N, Perlstein D. The Yeast Nbp35-Cfd1 Cytosolic Iron-Sulfur Cluster Scaffold Is an ATPase. J Biol Chem. 2015;290:23793-802 pubmed publisher
    b>Nbp35 and Cfd1 are prototypical members of the MRP/Nbp35 class of iron-sulfur (FeS) cluster scaffolds that function to assemble nascent FeS clusters for transfer to FeS-requiring enzymes...