NAP1

Summary

Gene Symbol: NAP1
Description: histone chaperone NAP1
Alias: histone chaperone NAP1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Park Y, Luger K. The structure of nucleosome assembly protein 1. Proc Natl Acad Sci U S A. 2006;103:1248-53 pubmed
    ..To our knowledge, this is the first structure of a member of the large NAP family of proteins and suggests a mechanism by which the shuttling of histones to and from the nucleus is regulated. ..
  2. Del Rosario B, Pemberton L. Nap1 links transcription elongation, chromatin assembly, and messenger RNP complex biogenesis. Mol Cell Biol. 2008;28:2113-24 pubmed publisher
    ..We demonstrate that the conserved Saccharomyces cerevisiae histone chaperone Nap1 associates with chromatin...
  3. Kellogg D, Kikuchi A, Fujii Nakata T, Turck C, Murray A. Members of the NAP/SET family of proteins interact specifically with B-type cyclins. J Cell Biol. 1995;130:661-73 pubmed
    ..This approach has led to the identification of a 60-kD protein called NAP1 that interacts specifically with members of the cyclin B family...
  4. Andrews A, Downing G, Brown K, Park Y, Luger K. A thermodynamic model for Nap1-histone interactions. J Biol Chem. 2008;283:32412-8 pubmed publisher
    ..The yNap1 tails contribute synergistically to histone binding while the histone tails have a slightly repressive effect on binding. The (H3/H4)(2) tetramer binds DNA with higher affinity than it binds yNap1. ..
  5. Andrews A, Chen X, Zevin A, Stargell L, Luger K. The histone chaperone Nap1 promotes nucleosome assembly by eliminating nonnucleosomal histone DNA interactions. Mol Cell. 2010;37:834-42 pubmed publisher
    ..Furthermore, our studies reveal the mechanism of action of the histone chaperone nucleosome assembly protein 1 (Nap1)...
  6. Straube K, Blackwell J, Pemberton L. Nap1 and Chz1 have separate Htz1 nuclear import and assembly functions. Traffic. 2010;11:185-97 pubmed publisher
    ..Z), and the role of histone chaperones Nap1 and Chz1 in this process. Copurification suggested that Htz1 and H2B dimerized in the cytoplasm prior to import...
  7. Park Y, Sudhoff K, Andrews A, Stargell L, Luger K. Histone chaperone specificity in Rtt109 activation. Nat Struct Mol Biol. 2008;15:957-64 pubmed
    ..We demonstrate that Vps75 is a structural homolog of yeast Nap1 by solving its crystal structure. Nap1 and Vps75 interact with histones and Rtt109 with comparable affinities...
  8. Kellogg D, Murray A. NAP1 acts with Clb1 to perform mitotic functions and to suppress polar bud growth in budding yeast. J Cell Biol. 1995;130:675-85 pubmed
    b>NAP1 is a 60-kD protein that interacts specifically with mitotic cyclins in budding yeast and frogs. We have examined the ability of the yeast mitotic cyclin Clb2 to function in cells that lack NAP1...
  9. Xue Y, Kowalska A, Grabowska K, Przybyt K, Cichewicz M, Del Rosario B, et al. Histone chaperones Nap1 and Vps75 regulate histone acetylation during transcription elongation. Mol Cell Biol. 2013;33:1645-56 pubmed publisher
    ..The Saccharomyces cerevisiae proteins Nap1 and Vps75 are structurally related, evolutionarily conserved histone chaperones...

More Information

Publications56

  1. Morillo Huesca M, Maya D, Muñoz Centeno M, Singh R, Oreal V, Reddy G, et al. FACT prevents the accumulation of free histones evicted from transcribed chromatin and a subsequent cell cycle delay in G1. PLoS Genet. 2010;6:e1000964 pubmed publisher
  2. Ishimi Y, Kikuchi A. Identification and molecular cloning of yeast homolog of nucleosome assembly protein I which facilitates nucleosome assembly in vitro. J Biol Chem. 1991;266:7025-9 pubmed
    ..Proteins recognized by the anti-NAP-I antibody were also present in Xenopus oocytes and Drosophila cultured cells. Possible roles of NAP-I are discussed in relation to other nucleosome assembly proteins. ..
  3. McBryant S, Park Y, Abernathy S, Laybourn P, Nyborg J, Luger K. Preferential binding of the histone (H3-H4)2 tetramer by NAP1 is mediated by the amino-terminal histone tails. J Biol Chem. 2003;278:44574-83 pubmed
    ..Our results are consistent with recent mechanistic investigations of NAP1 and expand our understanding of the histone chaperone family of assembly factors.
  4. D Arcy S, Martin K, Panchenko T, Chen X, Bergeron S, Stargell L, et al. Chaperone Nap1 shields histone surfaces used in a nucleosome and can put H2A-H2B in an unconventional tetrameric form. Mol Cell. 2013;51:662-77 pubmed publisher
    ..chromatin is regulated by numerous factors, including histone chaperones such as nucleosome assembly protein 1 (Nap1). We use hydrogen-deuterium exchange coupled to mass spectrometry to characterize H2A-H2B and Nap1...
  5. McBryant S, Peersen O. Self-association of the yeast nucleosome assembly protein 1. Biochemistry. 2004;43:10592-9 pubmed
    ..Dissociation of the dimer requires a nearly complete denaturation event. ..
  6. Miyaji Yamaguchi M, Kato K, Nakano R, Akashi T, Kikuchi A, Nagata K. Involvement of nucleocytoplasmic shuttling of yeast Nap1 in mitotic progression. Mol Cell Biol. 2003;23:6672-84 pubmed
    Nucleosome assembly protein 1 (Nap1) is widely conserved from yeasts to humans and facilitates nucleosome formation in vitro as a histone chaperone...
  7. Noda M, Uchiyama S, McKay A, Morimoto A, Misawa S, Yoshida A, et al. Assembly states of the nucleosome assembly protein 1 (NAP-1) revealed by sedimentation velocity and non-denaturing MS. Biochem J. 2011;436:101-12 pubmed publisher
    ..The present study illustrates a rigorous procedure for the analysis of protein assembly and protein-protein interactions in solution. ..
  8. Kuryan B, Kim J, Tran N, Lombardo S, Venkatesh S, Workman J, et al. Histone density is maintained during transcription mediated by the chromatin remodeler RSC and histone chaperone NAP1 in vitro. Proc Natl Acad Sci U S A. 2012;109:1931-6 pubmed publisher
    ..requires the ATPase remodels structure of chromatin (RSC) and the histone chaperone nucleosome assembly protein 1 (NAP1). In the presence of NAP1, RSC generates a hexasome...
  9. Park Y, McBryant S, Luger K. A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. J Mol Biol. 2008;375:1076-85 pubmed
    The histone chaperone nucleosome assembly protein 1 (NAP1) is implicated in histone shuttling as well as nucleosome assembly and disassembly...
  10. Mizuguchi G, Shen X, Landry J, Wu W, Sen S, Wu C. ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex. Science. 2004;303:343-8 pubmed
    ..These findings define a previously unknown role for the adenosine triphosphate-dependent chromatin remodeling machinery. ..
  11. Altman R, Kellogg D. Control of mitotic events by Nap1 and the Gin4 kinase. J Cell Biol. 1997;138:119-30 pubmed
    ..In budding yeast, a protein called Nap1 binds to the mitotic cyclin Clb2, and Nap1 is required for the ability of Clb2 to induce specific mitotic events, ..
  12. Tjandra H, Compton J, Kellogg D. Control of mitotic events by the Cdc42 GTPase, the Clb2 cyclin and a member of the PAK kinase family. Curr Biol. 1998;8:991-1000 pubmed
    ..A protein called Nap1 is required in vivo for the activation of Gin4, and is able to bind to both Gin4 and Clb2...
  13. Mortensen E, McDonald H, Yates J, Kellogg D. Cell cycle-dependent assembly of a Gin4-septin complex. Mol Biol Cell. 2002;13:2091-105 pubmed
    ..We show that during mitosis Gin4 is assembled into a multiprotein complex that includes Nap1, Bni5, the septins, and at least two molecules of Gin4...
  14. Hodges A, Gloss L, Wyrick J. Residues in the Nucleosome Acidic Patch Regulate Histone Occupancy and Are Important for FACT Binding in Saccharomyces cerevisiae. Genetics. 2017;206:1339-1348 pubmed publisher
    ..In summary, we show that residues in the nucleosome acidic patch promote histone occupancy and are important for FACT binding to H2A-H2B dimers in yeast. ..
  15. Tian R, Hoa X, Lambert J, Pezacki J, Veres T, Figeys D. Development of a multiplexed microfluidic proteomic reactor and its application for studying protein-protein interactions. Anal Chem. 2011;83:4095-102 pubmed publisher
  16. Shields C, Taylor R, Nazarenus T, Cheatle J, Hou A, Tapprich A, et al. Saccharomyces cerevisiae Ats1p interacts with Nap1p, a cytoplasmic protein that controls bud morphogenesis. Curr Genet. 2003;44:184-94 pubmed
    ..Our results suggest Ats1p might regulate coordination of the microtubule state with the cell cycle through an interaction with Nap1p. ..
  17. Bowman A, Hammond C, Stirling A, Ward R, Shang W, El Mkami H, et al. The histone chaperones Vps75 and Nap1 form ring-like, tetrameric structures in solution. Nucleic Acids Res. 2014;42:6038-51 pubmed publisher
    ..The two NAP-1 fold histone chaperones in budding yeast, Vps75 and Nap1, have previously been crystalized in a characteristic homodimeric conformation...
  18. Wang A, Aristizabal M, Ryan C, Krogan N, Kobor M. Key functional regions in the histone variant H2A.Z C-terminal docking domain. Mol Cell Biol. 2011;31:3871-84 pubmed publisher
    ..Z deposition complex SWR1-C, the histone chaperone Chz1, and histone H2B. These data are consistent with a model in which retaining the variant in chromatin after its deposition by SWR1-C is a crucial determinant of its function. ..
  19. Shimizu Y, Akashi T, Okuda A, Kikuchi A, Fukui K. NBP1 (Nap1 binding protein 1), an essential gene for G2/M transition of Saccharomyces cerevisiae, encodes a protein of distinct sub-nuclear localization. Gene. 2000;246:395-404 pubmed
    ..we have surveyed for proteins to interact with Nap1p by two-hybrid system and obtained two genes, NBP1 and NBP2 (Nap1 Binding Protein 1 and 2)...
  20. Prasad R, D Arcy S, Hada A, Luger K, Bartholomew B. Coordinated Action of Nap1 and RSC in Disassembly of Tandem Nucleosomes. Mol Cell Biol. 2016;36:2262-71 pubmed publisher
    ..We found that the histone chaperone Nap1 efficiently promotes disassembly of adjacent nucleosomes with which RSC collides and not the disassembly ..
  21. Lorch Y, Maier Davis B, Kornberg R. Chromatin remodeling by nucleosome disassembly in vitro. Proc Natl Acad Sci U S A. 2006;103:3090-3 pubmed
    The RSC chromatin-remodeling complex completely disassembles a nucleosome in the presence of the histone chaperone Nap1 and ATP...
  22. Zabel R, Bär C, Mehlgarten C, Schaffrath R. Yeast alpha-tubulin suppressor Ats1/Kti13 relates to the Elongator complex and interacts with Elongator partner protein Kti11. Mol Microbiol. 2008;69:175-87 pubmed publisher
    ..The Ats1/Kti13 gene product interacts with the cell polarity factor Nap1 and promotes growth inhibition of S. cerevisiae by zymocin, a tRNAse toxin complex from Kluyveromyces lactis...
  23. Mosammaparast N, Jackson K, Guo Y, Brame C, Shabanowitz J, Hunt D, et al. Nuclear import of histone H2A and H2B is mediated by a network of karyopherins. J Cell Biol. 2001;153:251-62 pubmed
    ..These data suggest that the import of H2A and H2B is mediated by a network of Kaps, in which Kap114p may play the major role. ..
  24. Longtine M, Theesfeld C, McMillan J, Weaver E, Pringle J, Lew D. Septin-dependent assembly of a cell cycle-regulatory module in Saccharomyces cerevisiae. Mol Cell Biol. 2000;20:4049-61 pubmed
    ..a kinase related to the Schizosaccharomyces pombe mitotic inducer Nim1p; CLA4, encoding a p21-activated kinase; and NAP1, encoding a Clb2p-interacting protein) also produce perturbations of septin organization associated with an Swe1p-..
  25. Fujii Nakata T, Ishimi Y, Okuda A, Kikuchi A. Functional analysis of nucleosome assembly protein, NAP-1. The negatively charged COOH-terminal region is not necessary for the intrinsic assembly activity. J Biol Chem. 1992;267:20980-6 pubmed
    ..This minimal size fragment could form the 12 S NAP-1-histone complex as the whole protein could, whereas deleted fragments on either side could bind with core histones only to form aggregates. ..
  26. Smart S, Mackintosh S, Edmondson R, Taverna S, Tackett A. Mapping the local protein interactome of the NuA3 histone acetyltransferase. Protein Sci. 2009;18:1987-97 pubmed publisher
    ..These novel, physical associations with yFACT, RSC, and Nap1 provide insight into the mechanism of NuA3-associated transcription and chromatin regulation.
  27. Carroll C, Altman R, Schieltz D, Yates J, Kellogg D. The septins are required for the mitosis-specific activation of the Gin4 kinase. J Cell Biol. 1998;143:709-17 pubmed
    ..These results demonstrate that the septins in budding yeast play an integral role in the mitosis-specific regulation of the Gin4 kinase and that they carry out functions early in mitosis. ..
  28. Meitinger F, Khmelinskii A, Morlot S, Kurtulmus B, Palani S, Andrés Pons A, et al. A memory system of negative polarity cues prevents replicative aging. Cell. 2014;159:1056-1069 pubmed publisher
    ..Our work thus established CRMs as negative polarity cues that prevent Cdc42 reactivation to sustain the fitness of replicating cells. ..
  29. Keck K, Pemberton L. Interaction with the histone chaperone Vps75 promotes nuclear localization and HAT activity of Rtt109 in vivo. Traffic. 2011;12:826-39 pubmed publisher
    ..Rtt109 associates with and is stabilized by Nap1 family histone chaperone Vps75...
  30. Hodges J, Leslie J, Mosammaparast N, Guo Y, Shabanowitz J, Hunt D, et al. Nuclear import of TFIIB is mediated by Kap114p, a karyopherin with multiple cargo-binding domains. Mol Biol Cell. 2005;16:3200-10 pubmed
    ..The import of more than one cargo at a time would increase the efficiency of each import cycle and may allow the regulation of coimported cargoes. ..
  31. Mosammaparast N, Del Rosario B, Pemberton L. Modulation of histone deposition by the karyopherin kap114. Mol Cell Biol. 2005;25:1764-78 pubmed
    ..Our results indicate a novel mechanism by which cells can regulate histone deposition and establish a coordinate link between histone nuclear import and chromatin assembly. ..
  32. Dronamraju R, Ramachandran S, Jha D, Adams A, DiFiore J, Parra M, et al. Redundant Functions for Nap1 and Chz1 in H2A.Z Deposition. Sci Rep. 2017;7:10791 pubmed publisher
    ..Z is deposited by the SWR-C complex, which relies on several histone chaperones including Nap1 and Chz1 to deliver H2A.Z-H2B dimers to SWR-C. However, the mechanisms by which Nap1 and Chz1 cooperate to bind H2A...
  33. Liang D, Burkhart S, Singh R, Kabbaj M, Gunjan A. Histone dosage regulates DNA damage sensitivity in a checkpoint-independent manner by the homologous recombination pathway. Nucleic Acids Res. 2012;40:9604-20 pubmed publisher
    ..Our findings may have important implications for DNA repair, genomic stability, carcinogenesis and aging in human cells that have dozens of histone genes. ..
  34. Kobor M, Venkatasubrahmanyam S, Meneghini M, Gin J, Jennings J, Link A, et al. A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin. PLoS Biol. 2004;2:E131 pubmed
    ..Metazoan orthologs of Swr1p (Drosophila Domino; human SRCAP and p400) may have analogous functions...
  35. Zimmerman Z, Kellogg D. The Sda1 protein is required for passage through start. Mol Biol Cell. 2001;12:201-19 pubmed
    We have used affinity chromatography to identify proteins that interact with Nap1, a protein previously shown to play a role in mitosis...
  36. Iwase M, Toh e A. Nis1 encoded by YNL078W: a new neck protein of Saccharomyces cerevisiae. Genes Genet Syst. 2001;76:335-43 pubmed
    ..Neither disruption nor overexpression of NIS1 caused a prominent phenotypic change. NAP1 was isolated by two-hybrid screening using NIS1 as bait...
  37. Mosammaparast N, Ewart C, Pemberton L. A role for nucleosome assembly protein 1 in the nuclear transport of histones H2A and H2B. EMBO J. 2002;21:6527-38 pubmed
    ..We propose a model in which Nap1p links the nuclear transport of H2A and H2B to chromatin assembly. ..
  38. Souid A, Gao C, Wang L, Milgrom E, Shen W. ELM1 is required for multidrug resistance in Saccharomyces cerevisiae. Genetics. 2006;173:1919-37 pubmed
    ..Collectively, these data show that ELM1, and genes encoding related serine/threonine protein kinases, are required for regulation of multidrug resistance involving, at least in part, control of PDR5 transcription...
  39. Ohkuni K, Okuda A, Kikuchi A. Yeast Nap1-binding protein Nbp2p is required for mitotic growth at high temperatures and for cell wall integrity. Genetics. 2003;165:517-29 pubmed
    Nbp2p is a Nap1-binding protein in Saccharomyces cerevisiae identified by its interaction with Nap1 by a two-hybrid system. NBP2 encodes a novel protein consisting of 236 amino acids with a Src homology 3 (SH3) domain...
  40. Hieb A, D Arcy S, Kramer M, White A, Luger K. Fluorescence strategies for high-throughput quantification of protein interactions. Nucleic Acids Res. 2012;40:e33 pubmed publisher
    ..to describe the preference of linker histone H1 for nucleosomes over DNA, the ionic dependence of the DNA repair enzyme PARP1 in DNA binding, and the interaction between the histone chaperone Nap1 and the histone H2A-H2B heterodimer.
  41. Tessarz P, Santos Rosa H, Robson S, Sylvestersen K, Nelson C, Nielsen M, et al. Glutamine methylation in histone H2A is an RNA-polymerase-I-dedicated modification. Nature. 2014;505:564-8 pubmed publisher
    ..Together these data identify glutamine methylation of H2A as the first histone epigenetic mark dedicated to a specific RNA polymerase and define its function as a regulator of FACT interaction with nucleosomes. ..
  42. Chen X, D Arcy S, Radebaugh C, Krzizike D, Giebler H, Huang L, et al. Histone Chaperone Nap1 Is a Major Regulator of Histone H2A-H2B Dynamics at the Inducible GAL Locus. Mol Cell Biol. 2016;36:1287-96 pubmed publisher
    Histone chaperones, like nucleosome assembly protein 1 (Nap1), play a critical role in the maintenance of chromatin architecture...
  43. Aguilar Gurrieri C, Larabi A, Vinayachandran V, Patel N, Yen K, Reja R, et al. Structural evidence for Nap1-dependent H2A-H2B deposition and nucleosome assembly. EMBO J. 2016;35:1465-82 pubmed publisher
    b>Nap1 is a histone chaperone involved in the nuclear import of H2A-H2B and nucleosome assembly...
  44. Iwase M, Toh e A. Ybr267w is a new cytoplasmic protein belonging to the mitotic signaling network of Saccharomyces cerevisiae. Cell Struct Funct. 2004;29:1-15 pubmed
    ..In view of the fact that members belonging to the mitotic signaling network are localized to the bud neck, at least at some stage of the cell cycle, Rei1 is a unique component of this pathway. ..
  45. Calvert M, Keck K, Ptak C, Shabanowitz J, Hunt D, Pemberton L. Phosphorylation by casein kinase 2 regulates Nap1 localization and function. Mol Cell Biol. 2008;28:1313-25 pubmed
    In Saccharomyces cerevisiae, the evolutionarily conserved nucleocytoplasmic shuttling protein Nap1 is a cofactor for the import of histones H2A and H2B, a chromatin assembly factor and a mitotic factor involved in regulation of bud ..
  46. Luk E, Vu N, Patteson K, Mizuguchi G, Wu W, Ranjan A, et al. Chz1, a nuclear chaperone for histone H2AZ. Mol Cell. 2007;25:357-68 pubmed
    ..In cells, the pool of unincorporated histone H2AZ has previously been found in association with Nap1, a chaperone for conventional histone H2A-H2B...
  47. Liu H, Zhu M, Mu Y, Liu L, Li G, Wan Y. Distinct roles for histone chaperones in the deposition of Htz1 in chromatin. Biosci Rep. 2014;34: pubmed publisher
    ..Histone chaperones Chz1 and Nap1 (nucleosome assembly protein 1) are important for the deposition Htz1 into nucleosomes...