Gene Symbol: NAM7
Description: ATP-dependent RNA helicase NAM7
Alias: IFS2, MOF4, SUP113, SUT2, UPF1, ATP-dependent RNA helicase NAM7
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Sheth U, Parker R. Targeting of aberrant mRNAs to cytoplasmic processing bodies. Cell. 2006;125:1095-109 pubmed
    ..Moreover, Upf1p can target normal mRNAs to P-bodies but not promote their degradation. These observations lead us to propose a new model for NMD wherein two successive steps are used to distinguish normal and aberrant mRNAs...
  2. Takahashi S, Araki Y, Sakuno T, Katada T. Interaction between Ski7p and Upf1p is required for nonsense-mediated 3'-to-5' mRNA decay in yeast. EMBO J. 2003;22:3951-9 pubmed
    ..These data suggest that the activation of 3'-to-5' directed NMD is mediated through the interaction between Upf1p and the Ski7p N domain. ..
  3. Mitchell P, Tollervey D. An NMD pathway in yeast involving accelerated deadenylation and exosome-mediated 3'-->5' degradation. Mol Cell. 2003;11:1405-13 pubmed
    ..We conclude that recognition of NMD substrates by the Upf surveillance complex can target mRNAs to rapid deadenylation and exosome-mediated degradation. ..
  4. Sayani S, Chanfreau G. Sequential RNA degradation pathways provide a fail-safe mechanism to limit the accumulation of unspliced transcripts in Saccharomyces cerevisiae. RNA. 2012;18:1563-72 pubmed publisher
    ..The presence of these two sequential degradation pathways for unspliced pre-mRNAs underscores the importance of limiting their accumulation and might serve as a fail-safe mechanism to prevent the expression of these nonfunctional RNAs. ..
  5. Ford A, Guan Q, Neeno Eckwall E, Culbertson M. Ebs1p, a negative regulator of gene expression controlled by the Upf proteins in the yeast Saccharomyces cerevisiae. Eukaryot Cell. 2006;5:301-12 pubmed
    ..Finally, EBS1 transcript levels are under the control of nonsense-mediated mRNA decay (NMD), providing the first example of an NMD-sensitive transcript whose protein product influences a step in gene expression required for NMD. ..
  6. Czaplinski K, Ruiz Echevarria M, Paushkin S, Han X, Weng Y, Perlick H, et al. The surveillance complex interacts with the translation release factors to enhance termination and degrade aberrant mRNAs. Genes Dev. 1998;12:1665-77 pubmed
    ..The product of the UPF1 gene is a necessary component of the putative surveillance complex that recognizes and degrades aberrant mRNAs...
  7. Kawashima T, Pellegrini M, Chanfreau G. Nonsense-mediated mRNA decay mutes the splicing defects of spliceosome component mutations. RNA. 2009;15:2236-47 pubmed publisher
    ..In addition, deletion of UPF1 partially suppresses the growth defects associated with the prp17Delta or prp22-1 mutations, demonstrating a ..
  8. Takahashi S, Araki Y, Ohya Y, Sakuno T, Hoshino S, Kontani K, et al. Upf1 potentially serves as a RING-related E3 ubiquitin ligase via its association with Upf3 in yeast. RNA. 2008;14:1950-8 pubmed publisher
    ..The Cys- and His-rich repeated N terminus (CH domain) of Upf1 has been implicated in its binding to Upf2...
  9. Zhou Y, Chen C, Johansson M. The pre-mRNA retention and splicing complex controls tRNA maturation by promoting TAN1 expression. Nucleic Acids Res. 2013;41:5669-78 pubmed publisher
    ..Our results demonstrate that TAN1 pre-mRNA processing has an unprecedented requirement for RES factors and that the complex controls the formation of ac(4)C in tRNA. ..

More Information


  1. Kobayashi T, Funakoshi Y, Hoshino S, Katada T. The GTP-binding release factor eRF3 as a key mediator coupling translation termination to mRNA decay. J Biol Chem. 2004;279:45693-700 pubmed
    ..the termination-coupled events, eRF3 interacts with poly(A)-binding protein (Pab1) and the surveillance factor Upf1 to mediate normal and nonsense-mediated mRNA decay...
  2. Wilson M, Meaux S, Parker R, van Hoof A. Genetic interactions between [PSI+] and nonstop mRNA decay affect phenotypic variation. Proc Natl Acad Sci U S A. 2005;102:10244-9 pubmed
    ..Such a process would allow periodic sampling of the 3' UTR, which can diverge rapidly, for novel and beneficial protein extensions. ..
  3. Wang W, Cajigas I, Peltz S, Wilkinson M, Gonzalez C. Role for Upf2p phosphorylation in Saccharomyces cerevisiae nonsense-mediated mRNA decay. Mol Cell Biol. 2006;26:3390-400 pubmed
    ..Two conserved proteins essential for NMD, UPF1 and UPF2, are phosphorylated in higher eukaryotes...
  4. De la Cruz J, Kressler D, Linder P. Unwinding RNA in Saccharomyces cerevisiae: DEAD-box proteins and related families. Trends Biochem Sci. 1999;24:192-8 pubmed
    ..Recent progress in the functional analysis of various family members has given new insights into, and confirmed the significance of these proteins for, most cellular RNA metabolic processes. ..
  5. Johansson M, Jacobson A. Nonsense-mediated mRNA decay maintains translational fidelity by limiting magnesium uptake. Genes Dev. 2010;24:1491-5 pubmed publisher
    ..Alr1p levels increase because an upstream ORF in ALR1 mRNA targets the transcript for NMD. Our results demonstrate that NMD, at least in yeast, controls Mg(2+) homeostasis and, consequently, translational fidelity. ..
  6. He F, Brown A, Jacobson A. Upf1p, Nmd2p, and Upf3p are interacting components of the yeast nonsense-mediated mRNA decay pathway. Mol Cell Biol. 1997;17:1580-94 pubmed
    ..nonsense-containing mRNAs in Saccharomyces cerevisiae is dependent on Upf1p, Nmd2p, and Upf3p, the products of the UPF1, NMD2/UPF2, and UPF3 genes, respectively...
  7. He F, Brown A, Jacobson A. Interaction between Nmd2p and Upf1p is required for activity but not for dominant-negative inhibition of the nonsense-mediated mRNA decay pathway in yeast. RNA. 1996;2:153-70 pubmed
    ..Rapid turnover of nonsense-containing mRNAs in the yeast Saccharomyces cerevisiae is dependent on the products of the UPF1 (Upf1p), NMD2/UPF2 (Nmd2p) and UPF3 (Upf3p) genes...
  8. Johansson M, He F, Spatrick P, Li C, Jacobson A. Association of yeast Upf1p with direct substrates of the NMD pathway. Proc Natl Acad Sci U S A. 2007;104:20872-7 pubmed
    ..we distinguished direct from indirect substrates by virtue of their association with the Saccharomyces cerevisiae Upf1 protein...
  9. Rutz B, Seraphin B. A dual role for BBP/ScSF1 in nuclear pre-mRNA retention and splicing. EMBO J. 2000;19:1873-86 pubmed
    ..A combination of ts mutants with a disruption of upf1, a gene involved in nonsense-mediated decay, resulted in a specific synthetic growth phenotype, suggesting that the ..
  10. Gonzalez C, Ruiz Echevarria M, Vasudevan S, Henry M, Peltz S. The yeast hnRNP-like protein Hrp1/Nab4 marks a transcript for nonsense-mediated mRNA decay. Mol Cell. 2000;5:489-99 pubmed
    ..A mutation in HRP1 that stabilizes nonsense-containing mRNAs abolishes its affinity for the DSE and fails to interact with Upf1p. We present a model describing how Hrp1p marks a transcript for rapid decay. ..
  11. Gaba A, Jacobson A, Sachs M. Ribosome occupancy of the yeast CPA1 upstream open reading frame termination codon modulates nonsense-mediated mRNA decay. Mol Cell. 2005;20:449-60 pubmed
    ..Improving the D13N uORF initiation context also promoted NMD. Hence, NMD appears to be triggered by increased ribosomal occupancy of the uORF termination codon. ..
  12. Nazarenus T, Cedarberg R, Bell R, Cheatle J, Forch A, Haifley A, et al. Upf1p, a highly conserved protein required for nonsense-mediated mRNA decay, interacts with the nuclear pore proteins Nup100p and Nup116p. Gene. 2005;345:199-212 pubmed
    ..Finally, we see a genetic interaction between UPF1 and NUP100. The growth of upf1Delta, can1-100 cells is inhibited by canavanine...
  13. Weng Y, Czaplinski K, Peltz S. Genetic and biochemical characterization of mutations in the ATPase and helicase regions of the Upf1 protein. Mol Cell Biol. 1996;16:5477-90 pubmed
    ..For the yeast Saccharomyces cerevisiae, the Upf1 protein (Upf1p), which contains a cysteine- and histidine-rich region and nucleoside triphosphate hydrolysis and ..
  14. He F, Jacobson A. Identification of a novel component of the nonsense-mediated mRNA decay pathway by use of an interacting protein screen. Genes Dev. 1995;9:437-54 pubmed
    Rapid turnover of nonsense-containing mRNAs in yeast in dependent on the product of the UPF1 gene (Upf1p)...
  15. Maderazo A, He F, Mangus D, Jacobson A. Upf1p control of nonsense mRNA translation is regulated by Nmd2p and Upf3p. Mol Cell Biol. 2000;20:4591-603 pubmed
    ..Deletion of UPF1, NMD2, or UPF3 stabilized the can1-100 transcript and promoted can1-100 nonsense suppression...
  16. Estrella L, Wilkinson M, Gonzalez C. The shuttling protein Npl3 promotes translation termination accuracy in Saccharomyces cerevisiae. J Mol Biol. 2009;394:410-22 pubmed publisher
    ..Together, these results lead us to propose a model in which Npl3p engenders translational fidelity by promoting the remodeling of mRNPs during translation termination. ..
  17. Kuroha K, Tatematsu T, Inada T. Upf1 stimulates degradation of the product derived from aberrant messenger RNA containing a specific nonsense mutation by the proteasome. EMBO Rep. 2009;10:1265-71 pubmed publisher
    ..Here, we show that a crucial NMD factor, up frameshift 1 protein (Upf1), is required for rapid proteasome-mediated degradation of an aberrant protein (PTC product) derived from a PTC-..
  18. Luke B, Azzalin C, Hug N, Deplazes A, Peter M, Lingner J. Saccharomyces cerevisiae Ebs1p is a putative ortholog of human Smg7 and promotes nonsense-mediated mRNA decay. Nucleic Acids Res. 2007;35:7688-97 pubmed
    ..Overall our findings suggest that NMD is more conserved in evolution than previously thought, and that at least one of the Smg5-7 proteins is conserved in budding yeast. ..
  19. Kervestin S, Li C, Buckingham R, Jacobson A. Testing the faux-UTR model for NMD: analysis of Upf1p and Pab1p competition for binding to eRF3/Sup35p. Biochimie. 2012;94:1560-71 pubmed publisher
    ..We conclude that activation of NMD at a premature termination codon is not solely based on the absence of Pab1p and suggest that a specific recruitment step must commit Upf1p to the process and Upf1p-associated mRNAs to NMD. ..
  20. Weng Y, Czaplinski K, Peltz S. Identification and characterization of mutations in the UPF1 gene that affect nonsense suppression and the formation of the Upf protein complex but not mRNA turnover. Mol Cell Biol. 1996;16:5491-506 pubmed
    ..In the yeast Saccharomyces cerevisiae, the Upf1 protein (Upf1p), which contains a cysteine- and histidine-rich region and nucleoside triphosphate hydrolysis and ..
  21. Penheiter K, Washburn T, Porter S, Hoffman M, Jaehning J. A posttranscriptional role for the yeast Paf1-RNA polymerase II complex is revealed by identification of primary targets. Mol Cell. 2005;20:213-23 pubmed
    ..MAK21 RNA is sensitive to nonsense-mediated decay (NMD), as revealed by its increased abundance in the absence of Upf1. Therefore, although the Paf1C is associated with Pol II at initiation and during elongation, these critical Paf1-..
  22. Wang W, Czaplinski K, Rao Y, Peltz S. The role of Upf proteins in modulating the translation read-through of nonsense-containing transcripts. EMBO J. 2001;20:880-90 pubmed
    The yeast UPF1, UPF2 and UPF3 genes encode trans-acting factors of the nonsense-mediated mRNA decay pathway...
  23. Culbertson M. RNA surveillance. Unforeseen consequences for gene expression, inherited genetic disorders and cancer. Trends Genet. 1999;15:74-80 pubmed
    ..The NMD pathway has a direct impact on hundreds of genetic disorders in the human population, where about a quarter of all known mutations are predicted to trigger NMD...
  24. de Pinto B, Lippolis R, Castaldo R, Altamura N. Overexpression of Upf1p compensates for mitochondrial splicing deficiency independently of its role in mRNA surveillance. Mol Microbiol. 2004;51:1129-42 pubmed
    In yeast the UPF1, UPF2 and UPF3 genes encode three interacting factors involved in translation termination and nonsense-mediated mRNA decay (NMD). UPF1 plays a central role in both processes...
  25. Dahlseid J, Puziss J, Shirley R, Atkin A, Hieter P, Culbertson M. Accumulation of mRNA coding for the ctf13p kinetochore subunit of Saccharomyces cerevisiae depends on the same factors that promote rapid decay of nonsense mRNAs. Genetics. 1998;150:1019-35 pubmed
    ..Four genes, UPF1, UPF2, UPF3, and ICK1, were represented among extragenic suppressors of ctf13-30...
  26. Min E, Roy B, Amrani N, He F, Jacobson A. Yeast Upf1 CH domain interacts with Rps26 of the 40S ribosomal subunit. RNA. 2013;19:1105-15 pubmed publisher
    The central nonsense-mediated mRNA decay (NMD) regulator, Upf1, selectively targets nonsense-containing mRNAs for rapid degradation...
  27. Neves L, Douglass S, Spreafico R, Venkataramanan S, Kress T, Johnson T. The histone variant H2A.Z promotes efficient cotranscriptional splicing in S. cerevisiae. Genes Dev. 2017;31:702-717 pubmed publisher
    ..Together, these data demonstrate that H2A.Z is required for efficient pre-mRNA splicing and indicate a role for H2A.Z in coordinating the kinetics of transcription elongation and splicing. ..
  28. Kvas S, Gloor G, Brandl C. Loss of nonsense mediated decay suppresses mutations in Saccharomyces cerevisiae TRA1. BMC Genet. 2012;13:19 pubmed publisher
    ..Using whole genome sequencing we identified one of the mutations as an opal mutation at tryptophan 165 of UPF1/NAM7...
  29. Harger J, Dinman J. Evidence against a direct role for the Upf proteins in frameshifting or nonsense codon readthrough. RNA. 2004;10:1721-9 pubmed
    ..The demonstration that upf sui1 double mutants are synthetically lethal demonstrates an important functional interaction between the NMD and translation initiation pathway. ..
  30. Messenguy F, Vierendeels F, Pierard A, Delbecq P. Role of RNA surveillance proteins Upf1/CpaR, Upf2 and Upf3 in the translational regulation of yeast CPA1 gene. Curr Genet. 2002;41:224-31 pubmed
    ..In this work, we show that the CPAR gene is identical to the UPF1 gene, encoding a protein responsible for the premature termination step of RNA surveillance by nonsense-mediated ..
  31. Swisher K, Parker R. Interactions between Upf1 and the decapping factors Edc3 and Pat1 in Saccharomyces cerevisiae. PLoS ONE. 2011;6:e26547 pubmed publisher
    ..Critical factors in NMD include Upf1, Upf2, and Upf3, as well as the decapping enzyme, Dcp2/Dcp1...
  32. Khmelinskii A, Blaszczak E, Pantazopoulou M, Fischer B, Omnus D, Le Dez G, et al. Protein quality control at the inner nuclear membrane. Nature. 2014;516:410-3 pubmed publisher
    ..We show that the Asi ubiquitin ligase is involved in degradation of mislocalized integral membrane proteins, thus acting to maintain and safeguard the identity of the INM. ..
  33. Barnes C. Upf1 and Upf2 proteins mediate normal yeast mRNA degradation when translation initiation is limited. Nucleic Acids Res. 1998;26:2433-41 pubmed
    ..This nonsense-mediated mRNA decay in the yeast Saccharomyces cerevisiae is mediated by the Upf1 and Upf2 proteins. Yeast mRNAs can also be selectively destabilized by limiting the rate of translation initiation...
  34. Zuk D, Belk J, Jacobson A. Temperature-sensitive mutations in the Saccharomyces cerevisiae MRT4, GRC5, SLA2 and THS1 genes result in defects in mRNA turnover. Genetics. 1999;153:35-47 pubmed
    ..The growth and mRNA decay defects of ts942 (grc5) cells were suppressed by overexpression of the NMD3 gene, encoding a protein shown to participate in a two-hybrid interaction with the nonsense-mediated decay protein Upf1p. ..
  35. Zheng W, Finkel J, Landers S, Long R, Culbertson M. Nonsense-mediated decay of ash1 nonsense transcripts in Saccharomyces cerevisiae. Genetics. 2008;180:1391-405 pubmed publisher
    ..Overall, our results suggest a model in which ash1 mRNAs are insensitive to NMD while translation is repressed during transport but become sensitive once repression is relieved. ..
  36. Sugiyama T, Nobuta R, Ando K, Matsuki Y, Inada T. Crucial role of ATP-bound Sse1 in Upf1-dependent degradation of the truncated product. Biochem Biophys Res Commun. 2017;488:122-128 pubmed publisher
    ..We propose that the Sse1/Hsp70 complex maintains the solubility of FLAG-Pgk1-300, thereby stimulating its Upf-dependent degradation by the proteasomes. ..
  37. Janke R, Kong J, Braberg H, Cantin G, Yates J, Krogan N, et al. Nonsense-mediated decay regulates key components of homologous recombination. Nucleic Acids Res. 2016;44:5218-30 pubmed publisher
  38. Das S, Saha U, Das B. Cbc2p, Upf3p and eIF4G are components of the DRN (Degradation of mRNA in the Nucleus) in Saccharomyces cerevisiae. FEMS Yeast Res. 2014;14:922-32 pubmed publisher
    ..These findings clearly establish that CBC2, UPF3, and TIF4631/32 gene products participate in DRN function. ..
  39. Chabelskaya S, Gryzina V, Moskalenko S, Le Goff C, Zhouravleva G. Inactivation of NMD increases viability of sup45 nonsense mutants in Saccharomyces cerevisiae. BMC Mol Biol. 2007;8:71 pubmed
    ..synthesis but also in mRNA degradation and translation initiation via interaction with such proteins as Pab1, Upf1, Upf2 and Upf3. In this work we have used previously isolated sup45 mutants of S...
  40. Enomoto S, Glowczewski L, Lew Smith J, Berman J. Telomere cap components influence the rate of senescence in telomerase-deficient yeast cells. Mol Cell Biol. 2004;24:837-45 pubmed
    ..Saccharomyces cerevisiae cells lacking components of the nonsense-mediated mRNA decay (NMD) pathway (Upf1,Upf2, or Upf3 proteins), senescence is delayed, with crisis occurring approximately 10 to 25 population doublings ..
  41. Deliz Aguirre R, Atkin A, Kebaara B. Copper tolerance of Saccharomyces cerevisiae nonsense-mediated mRNA decay mutants. Curr Genet. 2011;57:421-30 pubmed publisher
  42. He F, Jacobson A. Control of mRNA decapping by positive and negative regulatory elements in the Dcp2 C-terminal domain. RNA. 2015;21:1633-47 pubmed publisher
    ..The positive elements recruit the specific decapping activators Edc3, Pat1, and Upf1 to form distinct decapping complexes and control the enzyme's substrate specificity and final activation...
  43. Das B, Guo Z, Russo P, Chartrand P, Sherman F. The role of nuclear cap binding protein Cbc1p of yeast in mRNA termination and degradation. Mol Cell Biol. 2000;20:2827-38 pubmed
    ..CBP80 and CBP20 subunits of the nuclear cap binding complex, respectively, or by deletion of the nonessential gene UPF1, which encodes a major component of the mRNA surveillance complex...
  44. Kuroha K, Ando K, Nakagawa R, Inada T. The Upf factor complex interacts with aberrant products derived from mRNAs containing a premature termination codon and facilitates their proteasomal degradation. J Biol Chem. 2013;288:28630-40 pubmed publisher
    ..These findings suggest that the Upf complex may be recruited to ribosomes in a faux 3'-UTR-dependent manner and then associates with aberrant products to facilitate their degradation by the proteasome...
  45. Kodama H, Ito K, Nakamura Y. The role of N-terminal domain of translational release factor eRF3 for the control of functionality and stability in S. cerevisiae. Genes Cells. 2007;12:639-50 pubmed
    ..These findings suggest that NED functions to switch the functional mode of eRF3 depending on the nature of binding factors. ..
  46. Wang X, Okonkwo O, Kebaara B. Physiological basis of copper tolerance of Saccharomyces cerevisiae nonsense-mediated mRNA decay mutants. Yeast. 2013;30:179-90 pubmed publisher
    ..We found that wild-type (UPF1) and upf1? mutants accumulate similar amounts of total copper when grown in medium containing elevated levels of ..
  47. Huang S, Zhou H, Katzmann D, Hochstrasser M, Atanasova E, Zhang Z. Rtt106p is a histone chaperone involved in heterochromatin-mediated silencing. Proc Natl Acad Sci U S A. 2005;102:13410-5 pubmed
    ..The predicted gene products that are affected in three other mutants (nam7, msh2, and rtt106) have not been implicated previously in silencing...
  48. He F, Ganesan R, Jacobson A. Intra- and intermolecular regulatory interactions in Upf1, the RNA helicase central to nonsense-mediated mRNA decay in yeast. Mol Cell Biol. 2013;33:4672-84 pubmed publisher
    ..the stability and translational fidelity of nonsense-containing mRNAs are controlled by the group I RNA helicase Upf1 and the proteins it interacts with, Upf2 and Upf3...
  49. Kawashima T, Douglass S, Gabunilas J, Pellegrini M, Chanfreau G. Widespread use of non-productive alternative splice sites in Saccharomyces cerevisiae. PLoS Genet. 2014;10:e1004249 pubmed publisher
    ..cerevisiae but masked by RNA degradation and that the use of alternative splice sites in this organism is mostly aimed at controlling transcript levels rather than increasing proteome diversity. ..
  50. Ghosh S, Ganesan R, Amrani N, Jacobson A. Translational competence of ribosomes released from a premature termination codon is modulated by NMD factors. RNA. 2010;16:1832-47 pubmed publisher
    ..addition to their well-documented roles in the promotion of nonsense-mediated mRNA decay (NMD), yeast Upf proteins (Upf1, Upf2/Nmd2, and Upf3) also manifest translational regulatory functions, at least in vitro, including roles in ..
  51. Colin J, Candelli T, PorrĂșa O, Boulay J, Zhu C, Lacroute F, et al. Roadblock termination by reb1p restricts cryptic and readthrough transcription. Mol Cell. 2014;56:667-80 pubmed publisher
    ..This work demonstrates the importance of roadblock termination for controlling pervasive transcription and preventing transcription through gene regulatory regions. ..
  52. Dutko J, Kenny A, Gamache E, Curcio M. 5' to 3' mRNA decay factors colocalize with Ty1 gag and human APOBEC3G and promote Ty1 retrotransposition. J Virol. 2010;84:5052-66 pubmed publisher
    ..Together, the results suggest that VLPs assemble in P bodies and that 5' to 3' mRNA decay is essential for the packaging of Ty1 RNA in VLPs. ..
  53. Asher E, Groudinsky O, Dujardin G, Altamura N, Kermorgant M, Slonimski P. Novel class of nuclear genes involved in both mRNA splicing and protein synthesis in Saccharomyces cerevisiae mitochondria. Mol Gen Genet. 1989;215:517-28 pubmed
    We have cloned three distinct nuclear genes, NAM1, NAM7, and NAM8, which alleviate mitochondrial intron mutations of the cytochrome b and COXI (subunit I of cytochrome oxidase) genes when present on multicopy plasmids...
  54. Ma Z, Atencio D, Barnes C, Defiglio H, Hanes S. Multiple roles for the Ess1 prolyl isomerase in the RNA polymerase II transcription cycle. Mol Cell Biol. 2012;32:3594-607 pubmed publisher
    ..Thus, Ess1 has direct effects on RNA polymerase transcription by controlling cofactor binding via conformationally induced changes in the CTD and indirect effects by influencing chromatin modification. ..
  55. Lasalde C, Rivera A, Le n A, Gonz lez Feliciano J, Estrella L, Rodr guez Cruz E, et al. Identification and functional analysis of novel phosphorylation sites in the RNA surveillance protein Upf1. Nucleic Acids Res. 2014;42:1916-29 pubmed publisher
    ..A central component of the NMD pathway is Upf1, an RNA-dependent ATPase and helicase...
  56. He F, Jacobson A. Upf1p, Nmd2p, and Upf3p regulate the decapping and exonucleolytic degradation of both nonsense-containing mRNAs and wild-type mRNAs. Mol Cell Biol. 2001;21:1515-30 pubmed
    ..the NMD factors, we analyzed the mRNA decay phenotypes of yeast strains containing deletions of DCP1 or XRN1 and UPF1, NMD2, or UPF3...
  57. Zhuravleva G, Gryzina V. [The influence of UPF genes on the severity of SUP45 mutations]. Mol Biol (Mosk). 2012;46:285-97 pubmed
    ..of the PTC by the translational machinery and interaction of translation termination factors eRF1 and eRF3 with Upf1, Upf2 and Upf3 proteins. Previously we have shown that decreasing of eRF1 amount causes an impairment of NMD...
  58. Belk J, He F, Jacobson A. Overexpression of truncated Nmd3p inhibits protein synthesis in yeast. RNA. 1999;5:1055-70 pubmed
    ..Our data suggest that Nmd3p has a role in the formation, function, or maintenance of the 60S ribosomal subunit and may provide a link for Upf1p to 80S monosomes. ..
  59. Bond A, Mangus D, He F, Jacobson A. Absence of Dbp2p alters both nonsense-mediated mRNA decay and rRNA processing. Mol Cell Biol. 2001;21:7366-79 pubmed
  60. Taoka M, Yamauchi Y, Nobe Y, Masaki S, Nakayama H, Ishikawa H, et al. An analytical platform for mass spectrometry-based identification and chemical analysis of RNA in ribonucleoprotein complexes. Nucleic Acids Res. 2009;37:e140 pubmed publisher