NAB2

Summary

Gene Symbol: NAB2
Description: mRNA-binding protein NAB2
Alias: mRNA-binding protein NAB2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Schmid M, Poulsen M, Olszewski P, Pelechano V, Saguez C, Gupta I, et al. Rrp6p controls mRNA poly(A) tail length and its decoration with poly(A) binding proteins. Mol Cell. 2012;47:267-80 pubmed publisher
    ..We suggest that a nuclear mRNP surveillance step involves targeting of Rrp6p by Nab2p-bound pA-tailed RNPs and that pre-mRNA abundance is regulated at this level. ..
  2. Viphakone N, Voisinet Hakil F, Minvielle Sebastia L. Molecular dissection of mRNA poly(A) tail length control in yeast. Nucleic Acids Res. 2008;36:2418-33 pubmed publisher
  3. Kelly S, Leung S, Apponi L, Bramley A, Tran E, Chekanova J, et al. Recognition of polyadenosine RNA by the zinc finger domain of nuclear poly(A) RNA-binding protein 2 (Nab2) is required for correct mRNA 3'-end formation. J Biol Chem. 2010;285:26022-32 pubmed publisher
    ..coupled processes, we defined the mode of polyadenosine RNA recognition for the conserved Saccharomyces cerevisiae Nab2 protein and assessed in vivo consequences caused by disruption of RNA binding...
  4. Siomi M, Fromont M, Rain J, Wan L, Wang F, Legrain P, et al. Functional conservation of the transportin nuclear import pathway in divergent organisms. Mol Cell Biol. 1998;18:4141-8 pubmed
    ..Together, these findings demonstrate that both the M9 signal and the nuclear import machinery utilized by the transportin pathway are conserved in evolution. ..
  5. Zheng C, Fasken M, Marshall N, Brockmann C, Rubinson M, Wente S, et al. Structural basis for the function of the Saccharomyces cerevisiae Gfd1 protein in mRNA nuclear export. J Biol Chem. 2010;285:20704-15 pubmed publisher
    ..In Saccharomyces cerevisiae, the conserved poly(A) RNA-binding protein, Nab2, facilitates NPC targeting of transcripts and also modulates poly(A) tail length...
  6. Iglesias N, Tutucci E, Gwizdek C, Vinciguerra P, Von Dach E, Corbett A, et al. Ubiquitin-mediated mRNP dynamics and surveillance prior to budding yeast mRNA export. Genes Dev. 2010;24:1927-38 pubmed publisher
    ..Here we report that Mex67 interacts directly with Nab2, an essential shuttling mRNA-binding protein required for export...
  7. Grant R, Marshall N, Yang J, Fasken M, Kelly S, Harreman M, et al. Structure of the N-terminal Mlp1-binding domain of the Saccharomyces cerevisiae mRNA-binding protein, Nab2. J Mol Biol. 2008;376:1048-59 pubmed publisher
    Nuclear abundant poly(A) RNA-binding protein 2 (Nab2) is an essential yeast heterogeneous nuclear ribonucleoprotein that modulates both mRNA nuclear export and poly(A) tail length...
  8. Green D, Johnson C, Hagan H, Corbett A. The C-terminal domain of myosin-like protein 1 (Mlp1p) is a docking site for heterogeneous nuclear ribonucleoproteins that are required for mRNA export. Proc Natl Acad Sci U S A. 2003;100:1010-5 pubmed
    ..This study identifies Nab2p as a heterogeneous nuclear ribonucleoprotein found in complex with Mlp1p and begins to delineate the path that mRNA travels from the chromatin to the nuclear pore. ..
  9. Vinciguerra P, Iglesias N, Camblong J, Zenklusen D, Stutz F. Perinuclear Mlp proteins downregulate gene expression in response to a defect in mRNA export. EMBO J. 2005;24:813-23 pubmed
    ..interacts with the shuttling mRNA-binding protein Nab2p and that loss of Mlps rescues the growth defect of yra1 and nab2 but not other mRNA export mutants...

More Information

Publications42

  1. Anderson J, Wilson S, Datar K, Swanson M. NAB2: a yeast nuclear polyadenylated RNA-binding protein essential for cell viability. Mol Cell Biol. 1993;13:2730-41 pubmed
    ..Here we characterize one of these proteins, NAB2. NAB2 is one of the major proteins associated with nuclear polyadenylated RNA in vivo, as detected by UV light-..
  2. Suntharalingam M, Alcázar Román A, Wente S. Nuclear export of the yeast mRNA-binding protein Nab2 is linked to a direct interaction with Gfd1 and to Gle1 function. J Biol Chem. 2004;279:35384-91 pubmed
    ..In Saccharomyces cerevisiae, Nab2 is an essential RNA-binding protein that shuttles between the nucleus and cytoplasm...
  3. Xu C, Henry P, Setya A, Henry M. In vivo analysis of nucleolar proteins modified by the yeast arginine methyltransferase Hmt1/Rmt1p. RNA. 2003;9:746-59 pubmed
    ..The observation that three nucleolar proteins are modified by Hmt1p but are not exported from the nucleolus implies an alternate role for arginine methylation. ..
  4. Dheur S, Nykamp K, Viphakone N, Swanson M, Minvielle Sebastia L. Yeast mRNA Poly(A) tail length control can be reconstituted in vitro in the absence of Pab1p-dependent Poly(A) nuclease activity. J Biol Chem. 2005;280:24532-8 pubmed
    ..Based on these findings, we propose that Nab2p is necessary and sufficient to regulate poly(A) tail length during de novo synthesis in yeast. ..
  5. Kelly S, Pabit S, Kitchen C, Guo P, Marfatia K, Murphy T, et al. Recognition of polyadenosine RNA by zinc finger proteins. Proc Natl Acad Sci U S A. 2007;104:12306-11 pubmed
    ..We have probed the nucleic acid binding properties of a yeast protein, Nab2, that contains this zinc finger motif...
  6. González Aguilera C, Tous C, Gómez González B, Huertas P, Luna R, Aguilera A. The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability. Mol Biol Cell. 2008;19:4310-8 pubmed publisher
  7. Green D, Marfatia K, Crafton E, Zhang X, Cheng X, Corbett A. Nab2p is required for poly(A) RNA export in Saccharomyces cerevisiae and is regulated by arginine methylation via Hmt1p. J Biol Chem. 2002;277:7752-60 pubmed
    ..In this study, we characterize the Saccharomyces cerevisiae Nab2 protein, which was first identified as a poly(A) RNA-binding protein (Anderson, J. T., Wilson, S. M., Datar, K. V...
  8. Wilson S, Datar K, Paddy M, Swedlow J, Swanson M. Characterization of nuclear polyadenylated RNA-binding proteins in Saccharomyces cerevisiae. J Cell Biol. 1994;127:1173-84 pubmed
    ..These results suggest that the Nab proteins may be required for packaging pre-mRNAs into ribonucleoprotein structures amenable to efficient nuclear RNA processing. ..
  9. Gallardo M, Luna R, Erdjument Bromage H, Tempst P, Aguilera A. Nab2p and the Thp1p-Sac3p complex functionally interact at the interface between transcription and mRNA metabolism. J Biol Chem. 2003;278:24225-32 pubmed
    ..Multicopy NAB2 also suppressed the RNA export defect of thp1Delta cells...
  10. Hector R, Nykamp K, Dheur S, Anderson J, Non P, Urbinati C, et al. Dual requirement for yeast hnRNP Nab2p in mRNA poly(A) tail length control and nuclear export. EMBO J. 2002;21:1800-10 pubmed
    ..Loss of NAB2 expression leads to hyperadenylation and nuclear accumulation of poly(A)(+) RNA but, in contrast to mRNA export ..
  11. Lee D, Aitchison J. Kap104p-mediated nuclear import. Nuclear localization signals in mRNA-binding proteins and the role of Ran and Rna. J Biol Chem. 1999;274:29031-7 pubmed
    ..The resulting ribonucleoprotein complexes are exported to the cytoplasm, where Kap104p rebinds to Nab2p and Nab4p/Hrp1p, contributing to their release from mRNA. ..
  12. Truant R, Fridell R, Benson R, Bogerd H, Cullen B. Identification and functional characterization of a novel nuclear localization signal present in the yeast Nab2 poly(A)+ RNA binding protein. Mol Cell Biol. 1998;18:1449-58 pubmed
    ..These data therefore identify a novel NLS sequence, active in both yeast and mammalian cells, that is functionally distinct from both basic and M9 NLS sequences. ..
  13. Fasken M, Stewart M, Corbett A. Functional significance of the interaction between the mRNA-binding protein, Nab2, and the nuclear pore-associated protein, Mlp1, in mRNA export. J Biol Chem. 2008;283:27130-43 pubmed publisher
    ..In Saccharomyces cerevisiae, the shuttling heterogeneous nuclear ribonucleoprotein, Nab2, which is essential for mRNA export, specifically recognizes poly(A) RNA and binds to the nuclear pore-associated ..
  14. Reuter L, Meinel D, Sträßer K. The poly(A)-binding protein Nab2 functions in RNA polymerase III transcription. Genes Dev. 2015;29:1565-75 pubmed publisher
    ..Here, we discovered by a genome-wide approach that Nab2, a poly(A)-binding protein important for correct poly(A) tail length and nuclear mRNA export, is present at all ..
  15. Schmid M, Olszewski P, Pelechano V, Gupta I, Steinmetz L, Jensen T. The Nuclear PolyA-Binding Protein Nab2p Is Essential for mRNA Production. Cell Rep. 2015;12:128-139 pubmed publisher
    ..We conclude that Nab2p is required to protect early mRNA and therefore constitutes a crucial nuclear mRNA biogenesis factor. ..
  16. Santos Pereira J, Herrero A, García Rubio M, Marin A, Moreno S, Aguilera A. The Npl3 hnRNP prevents R-loop-mediated transcription-replication conflicts and genome instability. Genes Dev. 2013;27:2445-58 pubmed publisher
    ..Therefore, our work demonstrates that mRNP factors are critical for genome integrity and opens the option of using them as therapeutic targets in anti-cancer treatment. ..
  17. Vitaliano Prunier A, Babour A, Hérissant L, Apponi L, Margaritis T, Holstege F, et al. H2B ubiquitylation controls the formation of export-competent mRNP. Mol Cell. 2012;45:132-9 pubmed publisher
    ..recruitment of the different Mex67 adaptors to mRNPs, the effect of Swd2 ubiquitylation is restricted to Yra1 and Nab2, which, in turn, controls poly(A) tail length...
  18. Inoue K, Mizuno T, Wada K, Hagiwara M. Novel RING finger proteins, Air1p and Air2p, interact with Hmt1p and inhibit the arginine methylation of Npl3p. J Biol Chem. 2000;275:32793-9 pubmed
    ..Thus, Air1p and Air2p may affect mRNA transport by regulating the arginine methylation state of heterogeneous nuclear ribonucleoproteins...
  19. Aibara S, Gordon J, Riesterer A, McLaughlin S, Stewart M. Structural basis for the dimerization of Nab2 generated by RNA binding provides insight into its contribution to both poly(A) tail length determination and transcript compaction in Saccharomyces cerevisiae. Nucleic Acids Res. 2017;45:1529-1538 pubmed publisher
    ..b>Nab2 functions in this process to control both mRNP compaction that facilitates movement through nuclear pore complexes ..
  20. Reuter L, Sträßer K. Falling for the dark side of transcription: Nab2 fosters RNA polymerase III transcription. Transcription. 2016;7:69-74 pubmed publisher
    ..One of the open questions of RNAPIII transcription is whether and how additional factors are involved. Recently, Nab2 was identified as the first messenger ribonucleoprotein particle (mRNP) biogenesis factor with a function in ..
  21. Senissar M, Le Saux A, Belgareh Touzé N, Adam C, Banroques J, Tanner N. The DEAD-box helicase Ded1 from yeast is an mRNP cap-associated protein that shuttles between the cytoplasm and nucleus. Nucleic Acids Res. 2014;42:10005-22 pubmed publisher
    ..We conclude that Ded1 is an mRNP cofactor of the cap complex that may function to remodel the different mRNPs and thereby regulate the expression of the mRNAs. ..
  22. González Aguilera C, Tous C, Babiano R, de la Cruz J, Luna R, Aguilera A. Nab2 functions in the metabolism of RNA driven by polymerases II and III. Mol Biol Cell. 2011;22:2729-40 pubmed publisher
    ..One important player in this interface is the poly(A)(+)-RNA-binding protein Nab2, which regulates the mRNA poly(A)(+)-tail length and export...
  23. Kerr S, Azzouz N, Fuchs S, Collart M, Strahl B, Corbett A, et al. The Ccr4-Not complex interacts with the mRNA export machinery. PLoS ONE. 2011;6:e18302 pubmed publisher
    ..that Ccr4-Not physically and functionally interacts with the heterogenous nuclear ribonucleoproteins (hnRNPs) Nab2 and Hrp1, and that the physical association depends on Hmt1 methyltransferase activity...
  24. Süel K, Gu H, Chook Y. Modular organization and combinatorial energetics of proline-tyrosine nuclear localization signals. PLoS Biol. 2008;6:e137 pubmed publisher
    ..The modular organization of the PY-NLS coupled with its combinatorial energetics lays a path to decode this diverse and evolvable signal for future comprehensive genome-scale identification of nuclear import substrates. ..
  25. Mart nez Lumbreras S, Santiveri C, Mirassou Y, Zorrilla S, P rez Ca adillas J. Two singular types of CCCH tandem zinc finger in Nab2p contribute to polyadenosine RNA recognition. Structure. 2013;21:1800-11 pubmed publisher
    ..Moreover, we describe a hypothetical mechanism for controlling poly(A) tail length with specific roles for TZF12, TZF34, and Zf5-7 domains...
  26. Brockmann C, Soucek S, Kuhlmann S, Mills Lujan K, Kelly S, Yang J, et al. Structural basis for polyadenosine-RNA binding by Nab2 Zn fingers and its function in mRNA nuclear export. Structure. 2012;20:1007-18 pubmed publisher
    ..regulation and efficient nuclear export of mature mRNPs both require the polyadenosine-RNA-binding protein, Nab2, which contains seven CCCH Zn fingers...
  27. Soniat M, Sampathkumar P, Collett G, Gizzi A, Banu R, Bhosle R, et al. Crystal structure of human Karyopherin ?2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2. J Struct Funct Genomics. 2013;14:31-5 pubmed publisher
    ..Kap104, the Kap?2 homolog in Saccharomyces cerevisiae, recognizes PY-NLSs in cargos Nab2, Hrp1, and Tfg2...
  28. Palancade B, Zuccolo M, Loeillet S, Nicolas A, Doye V. Pml39, a novel protein of the nuclear periphery required for nuclear retention of improper messenger ribonucleoparticles. Mol Biol Cell. 2005;16:5258-68 pubmed
    ..trapping of mRNAs transcribed from an intron-containing reporter and of the heterogenous nuclear ribonucleoprotein Nab2 within discrete nuclear domains...
  29. Meinel D, Burkert Kautzsch C, Kieser A, O Duibhir E, Siebert M, Mayer A, et al. Recruitment of TREX to the transcription machinery by its direct binding to the phospho-CTD of RNA polymerase II. PLoS Genet. 2013;9:e1003914 pubmed publisher
    ..In summary, we provide insight into how the phospho-code of the CTD directs mRNP formation and export through TREX recruitment...
  30. Adams R, Terry L, Wente S. Nucleoporin FG domains facilitate mRNP remodeling at the cytoplasmic face of the nuclear pore complex. Genetics. 2014;197:1213-24 pubmed publisher
    ..Moreover, these results provide key evidence that character and context play a direct role in FG domain function and mRNA export. ..
  31. Zander G, Hackmann A, Bender L, Becker D, Lingner T, Salinas G, et al. mRNA quality control is bypassed for immediate export of stress-responsive transcripts. Nature. 2016;: pubmed publisher
    ..In Saccharomyces cerevisiae, the shuttling RNA adaptor proteins Npl3, Gbp2, Hrb1 and Nab2 are loaded co-transcriptionally onto growing pre-mRNAs...
  32. Apponi L, Kelly S, Harreman M, Lehner A, Corbett A, Valentini S. An interaction between two RNA binding proteins, Nab2 and Pub1, links mRNA processing/export and mRNA stability. Mol Cell Biol. 2007;27:6569-79 pubmed
    ..In a yeast two-hybrid screen, we identified nuclear poly(A) binding protein 2 (Nab2) as being a Pub1-interacting protein...