MYO4

Summary

Gene Symbol: MYO4
Description: myosin 4
Alias: FUN22, SHE1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. ncbi Mother cell-specific HO expression in budding yeast depends on the unconventional myosin myo4p and other cytoplasmic proteins
    R P Jansen
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 84:687-97. 1996
  2. ncbi Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA
    R M Long
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Science 277:383-7. 1997
  3. pmc Primary structure and cellular localization of chicken brain myosin-V (p190), an unconventional myosin with calmodulin light chains
    E M Espreafico
    Department of Biology, Yale University, New Haven, Connecticut 06511
    J Cell Biol 119:1541-57. 1992
  4. pmc Multiple Myo4 motors enhance ASH1 mRNA transport in Saccharomyces cerevisiae
    Sunglan Chung
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
    J Cell Biol 189:755-67. 2010
  5. pmc The Khd1 protein, which has three KH RNA-binding motifs, is required for proper localization of ASH1 mRNA in yeast
    Kenji Irie
    Department of Molecular Biology, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya 464 8602, Japan
    EMBO J 21:1158-67. 2002
  6. pmc She3p possesses a novel activity required for ASH1 mRNA localization in Saccharomyces cerevisiae
    Sharon M Landers
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Eukaryot Cell 8:1072-83. 2009
  7. pmc The yeast class V myosins, Myo2p and Myo4p, are nonprocessive actin-based motors
    S L Reck-Peterson
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 153:1121-6. 2001
  8. ncbi Plasma membrane compartmentalization in yeast by messenger RNA transport and a septin diffusion barrier
    P A Takizawa
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA
    Science 290:341-4. 2000
  9. pmc Monomeric myosin V uses two binding regions for the assembly of stable translocation complexes
    Alexander Heuck
    Institute of Structural Biology, GSF National Research Center, 81377 Munich, Germany
    Proc Natl Acad Sci U S A 104:19778-83. 2007
  10. doi She3p binds to the rod of yeast myosin V and prevents it from dimerizing, forming a single-headed motor complex
    Alex R Hodges
    Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, Vermont 05405, USA
    J Biol Chem 283:6906-14. 2008

Scientific Experts

  • Hang Shi
  • Alexander Heuck
  • Elena B Krementsova
  • Carol S Bookwalter
  • Kathleen M Trybus
  • Alex R Hodges
  • P A Takizawa
  • Kenji Irie
  • Ralf Peter Jansen
  • Peter A Takizawa
  • Koji Makanae
  • Zane J Bergman
  • Rita Gelin-Licht
  • Jeffrey E Gerst
  • Anna Babour
  • Sunglan Chung
  • Tomofumi Tadauchi
  • Meghal Gandhi
  • Boris Slobodin
  • R P Jansen
  • Sharon M Landers
  • Dierk Niessing
  • Brian D Dunn
  • Roy M Long
  • Graydon B Gonsalvez
  • Istvan R Boldogh
  • R D Vale
  • Kunihiro Matsumoto
  • Paula Estrada
  • Hirofumi Toi
  • Stefanie Wesche
  • Reiko Kintaka
  • Hisao Moriya
  • Takashi Makino
  • Hiroaki Kitano
  • K Nasmyth
  • Tim C Huffaker
  • Andre Levchenko
  • S L Reck-Peterson
  • Patrick Conlon
  • Xue Xia
  • Saurabh Paliwal
  • I Alexandra Amaro
  • Mirko Travaglia
  • Thomas E Sladewski
  • H Lee Sweeney
  • Mary Munson
  • Daniel N Brewer
  • Joel Tourtellotte
  • Alicia A Bicknell
  • Daniela Hüls
  • Mohini Jangi
  • Ingrid Fetka
  • F Bohl
  • Bruce L Goode
  • Maho Niwa
  • Michelle R Gallas
  • Jaime Little
  • Matthew Lord
  • S Münchow
  • M P Cosma
  • Klaus Richter
  • Takeshi Sakamoto
  • Sigrun Jaklin
  • Stephan Jellbauer
  • Myoung Soon S Hong
  • Tung Gia Du
  • Marisa Müller
  • James R Sellers
  • Johannes Buchner
  • Claudia Kruse
  • Toshifumi Inada
  • R M Long
  • Sharmilee L Ramcharan
  • Jaime L Little
  • Hyeong Cheol Yang
  • Liza A Pon
  • Susan Ferro-Novick
  • Brian Dunn
  • Peter Takizawa
  • Satoru Todo
  • Kazuma Tanaka
  • M S Mooseker
  • Konomi Fujimura-Kamada
  • Jeff Coleman
  • Yoshimi Takai
  • Marc Arnold
  • Peter Novick
  • Jiwon Kim
  • Lee Walker

Detail Information

Publications33

  1. ncbi Mother cell-specific HO expression in budding yeast depends on the unconventional myosin myo4p and other cytoplasmic proteins
    R P Jansen
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 84:687-97. 1996
    ..We describe the identification of five genes, called SHE1-SHE5, that encode cytoplasmic proteins required for mother-specific HO expression...
  2. ncbi Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA
    R M Long
    Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Science 277:383-7. 1997
    ..A cis-acting element in the 3'-untranslated region of ASH1 mRNA is sufficient to localize a chimeric RNA to daughter cells. These results suggest that localization of mRNA may have been an early property of the eukaryotic lineage...
  3. pmc Primary structure and cellular localization of chicken brain myosin-V (p190), an unconventional myosin with calmodulin light chains
    E M Espreafico
    Department of Biology, Yale University, New Haven, Connecticut 06511
    J Cell Biol 119:1541-57. 1992
    ..Immunolocalization of myosin-V in brain and in cultured cells revealed an unusual distribution for this myosin in both neurons and nonneuronal cells.(ABSTRACT TRUNCATED AT 400 WORDS)..
  4. pmc Multiple Myo4 motors enhance ASH1 mRNA transport in Saccharomyces cerevisiae
    Sunglan Chung
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
    J Cell Biol 189:755-67. 2010
    In Saccharomyces cerevisiae, ASH1 mRNA is transported to the bud tip by the class V myosin Myo4. In vivo, Myo4 moves RNA in a rapid and continuous fashion, but in vitro Myo4 is a nonprocessive, monomeric motor that forms a complex with ..
  5. pmc The Khd1 protein, which has three KH RNA-binding motifs, is required for proper localization of ASH1 mRNA in yeast
    Kenji Irie
    Department of Molecular Biology, Graduate School of Science, Nagoya University, Chikusa ku, Nagoya 464 8602, Japan
    EMBO J 21:1158-67. 2002
    ..This localization depends on the actin cytoskeleton and five She proteins, one of which is a type V myosin motor, Myo4. We show here that a novel RNA-binding protein, Khd1 (KH-domain protein 1), is required for efficient localization ..
  6. pmc She3p possesses a novel activity required for ASH1 mRNA localization in Saccharomyces cerevisiae
    Sharon M Landers
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Eukaryot Cell 8:1072-83. 2009
    ..The present study reveals that the current accepted model for ASH1 mRNA localization does not fully account for the function of She3p in ASH1 mRNA localization...
  7. pmc The yeast class V myosins, Myo2p and Myo4p, are nonprocessive actin-based motors
    S L Reck-Peterson
    Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA
    J Cell Biol 153:1121-6. 2001
    ..These studies suggest that if Myo2p functions in organelle transport, at least five molecules of Myo2p must be present per organelle to promote directed movement...
  8. ncbi Plasma membrane compartmentalization in yeast by messenger RNA transport and a septin diffusion barrier
    P A Takizawa
    Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, University of California, San Francisco, CA 94143, USA
    Science 290:341-4. 2000
    ..These results indicate that yeast creates distinct plasma membrane compartments, as has been described in neurons and epithelial cells...
  9. pmc Monomeric myosin V uses two binding regions for the assembly of stable translocation complexes
    Alexander Heuck
    Institute of Structural Biology, GSF National Research Center, 81377 Munich, Germany
    Proc Natl Acad Sci U S A 104:19778-83. 2007
    ..This heterologous motor successfully translocates its cargo in vivo, suggesting that wild-type Myo4p may also function as a dimer during cargo-complex transport...
  10. doi She3p binds to the rod of yeast myosin V and prevents it from dimerizing, forming a single-headed motor complex
    Alex R Hodges
    Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, Vermont 05405, USA
    J Biol Chem 283:6906-14. 2008
    ..We propose that continuous transport of mRNA by Myo4p-She3p in yeast is accomplished either by multiple high duty cycle monomers or by molecules that may be dimerized by She2p, the homodimeric downstream binding partner of She3p...
  11. pmc Myo4p is a monomeric myosin with motility uniquely adapted to transport mRNA
    Brian D Dunn
    Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06520, USA
    J Cell Biol 178:1193-206. 2007
    ..Our results suggest that the oligomeric state of Myo4p is important for its motility and ability to transport mRNA...
  12. ncbi Actin-dependent localization of an RNA encoding a cell-fate determinant in yeast
    P A Takizawa
    Howard Hughes Medical Institute, Department of Cellular and Molecular Pharmacology, University of California, San Francisco 94143 0450, USA
    Nature 389:90-3. 1997
    ..Ash1 mRNA is not localized correctly in the absence of a myosin (Myo4) and is mislocalized to the mother-bud neck in the absence of a regulator of the actin cytoskeleton known as Bnil...
  13. pmc The structure of the Myo4p globular tail and its function in ASH1 mRNA localization
    Alexander Heuck
    Institute of Structural Biology, Helmholtz Zentrum Munchen, German Research Center for Environmental Health, Munich, Germany
    J Cell Biol 189:497-510. 2010
    ..The structure shows pronounced similarities to membrane-tethering complexes and indicates that Myo4p may not undergo auto-inhibition of its motor domain...
  14. pmc Myo4p and She3p are required for cortical ER inheritance in Saccharomyces cerevisiae
    Paula Estrada
    Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06519, USA
    J Cell Biol 163:1255-66. 2003
    ..Additionally, a reduction in the number of cytoplasmic ER tubules was observed in Lat-A-treated and myo4Delta cells. Our results suggest that Myo4p and She3p facilitate the growth and orientation of ER tubules...
  15. ncbi Association of the class V myosin Myo4p with a localised messenger RNA in budding yeast depends on She proteins
    S Münchow
    ZMBH, University of Heidelberg, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
    J Cell Sci 112:1511-8. 1999
    ..This strongly suggests a direct role of Myo4p myosin as a transporter of localised mRNAs, convincingly supporting the concept of motor-protein based mRNA localisation...
  16. pmc She2p, a novel RNA-binding protein tethers ASH1 mRNA to the Myo4p myosin motor via She3p
    F Bohl
    ZMBH, University of Heidelberg, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
    EMBO J 19:5514-24. 2000
    ..She2p and She3p are the first examples of adapters for tethering a localized mRNA to the motor protein and might serve as prototypes for RNA-motor protein adapters...
  17. pmc The myosin motor, Myo4p, binds Ash1 mRNA via the adapter protein, She3p
    P A Takizawa
    Department of Cellular and Molecular Pharmacology and Howard Hughes Medical Institute, University of California, San Francisco, CA 94143 0450, USA
    Proc Natl Acad Sci U S A 97:5273-8. 2000
    ..These results suggest that She3p acts as an adapter protein that docks the myosin motor onto an Ash1-She2p ribonucleoprotein complex...
  18. ncbi Identification of MYO4, a second class V myosin gene in yeast
    B K Haarer
    Department of Anatomy and Cell Biology, University of Michigan Medical School, Ann Arbor 48109 0616
    J Cell Sci 107:1055-64. 1994
    We have isolated a fourth myosin gene (MYO4) in yeast (Saccharomyces cerevisiae). MYO4 encodes a approximately 170 kDa (1471 amino acid) class V myosin, using the classification devised by Cheney et al. (1993a; Cell Motil. Cytoskel...
  19. pmc Essential features of the class V myosin from budding yeast for ASH1 mRNA transport
    Carol S Bookwalter
    Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, VT 05405, USA
    Mol Biol Cell 20:3414-21. 2009
    ..Our results show that the most important feature for correct localization is the retention of coupling between all the members of the complex (Myo4p-She3p-She2p-ASH1 mRNA), which is aided by She3p being a tightly bound subunit of Myo4p...
  20. pmc Constitutive dynein activity in She1 mutants reveals differences in microtubule attachment at the yeast spindle pole body
    Zane J Bergman
    Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
    Mol Biol Cell 23:2319-26. 2012
    ..In Saccharomyces cerevisiae cells lacking She1, cytoplasmic microtubules detach from the spindle pole body at high rates...
  21. pmc Scp160-dependent mRNA trafficking mediates pheromone gradient sensing and chemotropism in yeast
    Rita Gelin-Licht
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    Cell Rep 1:483-94. 2012
    ..the Scp160 RNA-binding protein binds POL and mating pathway mRNAs and regulates their spatial distribution in a Myo4- and cortical ER-dependent fashion...
  22. pmc Two single-headed myosin V motors bound to a tetrameric adapter protein form a processive complex
    Elena B Krementsova
    Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, VT 05405, USA
    J Cell Biol 195:631-41. 2011
    ..Coupling two high-duty cycle monomeric motors via a common cargo-binding adapter protein creates a complex with transport properties comparable with a single dimeric processive motor such as vertebrate myosin Va...
  23. pmc Identification of dosage-sensitive genes in Saccharomyces cerevisiae using the genetic tug-of-war method
    Koji Makanae
    Research Core for Interdisciplinary Sciences, Okayama University, Okayama 700 8530, Japan
    Genome Res 23:300-11. 2013
    ..The results obtained in this study will provide basic knowledge about the physiology of chromosomal abnormalities and the evolution of chromosomal composition...
  24. ncbi ASH1 mRNA anchoring requires reorganization of the Myo4p-She3p-She2p transport complex
    Graydon B Gonsalvez
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 279:46286-94. 2004
    ..These results are consistent with a model whereby anchoring of ASH1 mRNA requires molecular remodeling of the Myo4p-She3p-She2p heterotrimeric complex, a process that is apparently altered when mRNA is artificially tethered to She2p...
  25. ncbi Ordered recruitment of transcription and chromatin remodeling factors to a cell cycle- and developmentally regulated promoter
    M P Cosma
    Research Institute of Molecular Pathology, Vienna, Austria
    Cell 97:299-311. 1999
    ..Swi5p remains at HO for only 5 min. Swi/Snf's and SAGA's subsequent persistence at HO is self sustaining and constitutes an "epigenetic memory" of HO's transient interaction with Swi5p...
  26. ncbi The UCS domain protein She4p binds to myosin motor domains and is essential for class I and class V myosin function
    Stefanie Wesche
    ZMBH, Universitat Heidelberg, Im Neuenheimer Feld 282, D 69120 Heidelberg, Germany
    Curr Biol 13:715-24. 2003
    ..The Saccharomyces cerevisae UCS domain protein She4p is involved in two myosin-requiring events, endocytosis and mRNA localization...
  27. pmc She4p/Dim1p interacts with the motor domain of unconventional myosins in the budding yeast, Saccharomyces cerevisiae
    Hirofumi Toi
    Division of Molecular Interaction, Institute for Genetic Medicine, Hokkaido University Graduate School of Medicine, Japan
    Mol Biol Cell 14:2237-49. 2003
    ..Our results suggest that She4p/Dim1p is required for structural integrity or regulation of the motor domain of unconventional myosins...
  28. pmc UNC-45/CRO1/She4p (UCS) protein forms elongated dimer and joins two myosin heads near their actin binding region
    Hang Shi
    Laboratory of Cell Biology, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA
    Proc Natl Acad Sci U S A 107:21382-7. 2010
    ..We propose that a UCS dimer links two myosins at their motor domains and thereby functions as one of the determinants for step size of myosin on actin filaments...
  29. pmc A novel mRNA affinity purification technique for the identification of interacting proteins and transcripts in ribonucleoprotein complexes
    Boris Slobodin
    Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
    RNA 16:2277-90. 2010
    ..Finally, RaPID could also be used to show biochemically the coexistence of different RNA species within the same RNP complex (e.g., coprecipitation of the yeast SRO7, WSC2, SEC3, and IST2 mRNAs with ASH1 mRNA) for the first time...
  30. pmc Functional surfaces on the actin-binding protein coronin revealed by systematic mutagenesis
    Meghal Gandhi
    Department of Biology, Rosenstiel Basic Medical Science Research Center, Brandeis University, Waltham, Massachusetts 02454, USA
    J Biol Chem 285:34899-908. 2010
    ..Conservation of the actin-binding surfaces across distant species and in all three major classes of coronin isoforms suggests that the nature of the coronin-actin association may be similar in other family members...
  31. pmc A surveillance pathway monitors the fitness of the endoplasmic reticulum to control its inheritance
    Anna Babour
    Division of Biological Sciences, Section of Molecular Biology, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093 0377, USA
    Cell 142:256-69. 2010
    ..Furthermore, Slt2 is activated via the cell surface receptor Wsc1 by a previously undescribed mechanism. We conclude that the ERSU pathway ensures inheritance of a functional ER...
  32. pmc Posttranscriptional regulation of HO expression by the Mkt1-Pbp1 complex
    Tomofumi Tadauchi
    Department of Molecular Biology, Graduate School of Science, Institute for Advanced Research, Nagoya University, and CREST, Japan Science and Technology Corporation, Chikusa ku, Nagoya, Japan
    Mol Cell Biol 24:3670-81. 2004
    ..These observations suggest that a complex of Mkt1 and Pbp1 regulates the translation of HO mRNA...
  33. pmc A type V myosin (Myo2p) and a Rab-like G-protein (Ypt11p) are required for retention of newly inherited mitochondria in yeast cells during cell division
    Istvan R Boldogh
    Department of Anatomy and Cell Biology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Mol Biol Cell 15:3994-4002. 2004
    ..We found that deletion of YPT11, reduction of the length of the Myo2p lever arm (myo2-Delta6IQ), or deletion of MYO4 (the other yeast myosin V), had no effect on mitochondrial morphology, colocalization of mitochondria with actin ..