MYO2

Summary

Gene Symbol: MYO2
Description: myosin 2
Alias: CDC66, myosin 2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Yin H, Pruyne D, Huffaker T, Bretscher A. Myosin V orientates the mitotic spindle in yeast. Nature. 2000;406:1013-5 pubmed
    ..Here we show that yeast Myo2, a myosin V that translocates along polarized actin cables into the bud, orientates the spindle early in the cell ..
  2. Wang T, Bretscher A. The rho-GAP encoded by BEM2 regulates cytoskeletal structure in budding yeast. Mol Biol Cell. 1995;6:1011-24 pubmed
    ..mutations also show synthetic lethality with rho1 and mutations in certain other cytoskeletal genes (ACT1, MYO1, MYO2, and SAC6) but not with mutations in several noncytoskeletal genes...
  3. Pashkova N, Catlett N, Novak J, Weisman L. A point mutation in the cargo-binding domain of myosin V affects its interaction with multiple cargoes. Eukaryot Cell. 2005;4:787-98 pubmed
    ..Here we analyze a point mutation in subdomain I, myo2-2(G1248D), which is likely to be this latter type of mutation. myo2-2 has no effect on secretory vesicle movement...
  4. Hoepfner D, Van den Berg M, Philippsen P, Tabak H, Hettema E. A role for Vps1p, actin, and the Myo2p motor in peroxisome abundance and inheritance in Saccharomyces cerevisiae. J Cell Biol. 2001;155:979-90 pubmed
    ..the actin cytoskeleton abolished all peroxisomal movements, and (d) in cells containing thermosensitive alleles of MYO2, all peroxisome movement immediately stopped at the nonpermissive temperature...
  5. Legesse Miller A, Zhang S, Santiago Tirado F, Van Pelt C, Bretscher A. Regulated phosphorylation of budding yeast's essential myosin V heavy chain, Myo2p. Mol Biol Cell. 2006;17:1812-21 pubmed
    ..These results suggest that in yeast, Myo2p is subject to phosphoregulation involving a PKA-related signaling pathway. ..
  6. Itoh T, Watabe A, Toh e A, Matsui Y. Complex formation with Ypt11p, a rab-type small GTPase, is essential to facilitate the function of Myo2p, a class V myosin, in mitochondrial distribution in Saccharomyces cerevisiae. Mol Cell Biol. 2002;22:7744-57 pubmed
    ..We isolated two myo2 mutants, myo2-338 and myo2-573, which showed genetic interactions with YPT11...
  7. Terrak M, Wu G, Stafford W, Lu R, Dominguez R. Two distinct myosin light chain structures are induced by specific variations within the bound IQ motifs-functional implications. EMBO J. 2003;22:362-71 pubmed
    ..The resulting 'free' N-lobes of myosin light chains in the extended conformation could mediate the formation of ternary complexes during protein localization and/or partner recruitment. ..
  8. Santiago Tirado F, Legesse Miller A, Schott D, Bretscher A. PI4P and Rab inputs collaborate in myosin-V-dependent transport of secretory compartments in yeast. Dev Cell. 2011;20:47-59 pubmed publisher
    ..Thus, we show that a coincidence detection mechanism coordinates inputs from PI4P and the appropriate Rab for secretory compartment transport. ..
  9. Reck Peterson S, Tyska M, Novick P, Mooseker M. The yeast class V myosins, Myo2p and Myo4p, are nonprocessive actin-based motors. J Cell Biol. 2001;153:1121-6 pubmed
    ..These studies suggest that if Myo2p functions in organelle transport, at least five molecules of Myo2p must be present per organelle to promote directed movement. ..

More Information

Publications75

  1. Beningo K, Lillie S, Brown S. The yeast kinesin-related protein Smy1p exerts its effects on the class V myosin Myo2p via a physical interaction. Mol Biol Cell. 2000;11:691-702 pubmed
    ..Deletion of this myosin-binding region of Smy1p eliminates its ability to colocalize with Myo2p and to overcome the myo2-66 mutant defects, suggesting that the interaction is necessary for these functions...
  2. Peng Y, Weisman L. The cyclin-dependent kinase Cdk1 directly regulates vacuole inheritance. Dev Cell. 2008;15:478-85 pubmed publisher
    ..and several other organelles is actin-based and is mediated by interaction between the yeast myosin V motor Myo2 and organelle-specific adaptors...
  3. Wagner W, Bielli P, Wacha S, Ragnini Wilson A. Mlc1p promotes septum closure during cytokinesis via the IQ motifs of the vesicle motor Myo2p. EMBO J. 2002;21:6397-408 pubmed
    ..Further more, myosin light chains, via their ability to be transported by secretory vesicles and to interact with class V myosin IQ motifs, can regulate vesicle polarization processes at a specific location and stage of the cell cycle. ..
  4. Luo J, Vallen E, Dravis C, Tcheperegine S, Drees B, Bi E. Identification and functional analysis of the essential and regulatory light chains of the only type II myosin Myo1p in Saccharomyces cerevisiae. J Cell Biol. 2004;165:843-55 pubmed
    ..We also identify Mlc2p as the regulatory light chain for Myo1p and demonstrate its role in Myo1p ring disassembly, a function likely conserved among eukaryotes. ..
  5. Donovan K, Bretscher A. Myosin-V is activated by binding secretory cargo and released in coordination with Rab/exocyst function. Dev Cell. 2012;23:769-81 pubmed publisher
    ..All components of this transport cycle are conserved in vertebrates, so these results should be generally applicable to other myosin-V delivery cycles. ..
  6. Lillie S, Brown S. Smy1p, a kinesin-related protein that does not require microtubules. J Cell Biol. 1998;140:873-83 pubmed
    ..We conclude that Smy1p does not act as a microtubule-based motor to localize properly or to compensate for defective Myo2p, but that it must instead act in some novel way. ..
  7. Tang F, Kauffman E, Novak J, Nau J, Catlett N, Weisman L. Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature. 2003;422:87-92 pubmed
    ..Thus the regulated disruption of this transport complex places the vacuole in its proper location. This may be a general mechanism whereby organelles are deposited at their terminal destination. ..
  8. Gangar A, Rossi G, Andreeva A, Hales R, Brennwald P. Structurally conserved interaction of Lgl family with SNAREs is critical to their cellular function. Curr Biol. 2005;15:1136-42 pubmed
    ..We report a novel interaction between Sro7 and the yeast myosin V, Myo2. However, we find that interactions with either Myo2 or Myo1 (myosin II) cannot account for the dramatic functional ..
  9. Lipatova Z, Tokarev A, Jin Y, Mulholland J, Weisman L, Segev N. Direct interaction between a myosin V motor and the Rab GTPases Ypt31/32 is required for polarized secretion. Mol Biol Cell. 2008;19:4177-87 pubmed publisher
    ..In yeast, the Ypt31/32 functional pair is required for the formation of trans-Golgi vesicles. The myosin V motor Myo2 attaches to these vesicles through its globular-tail domain (GTD) and mediates their polarized delivery to sites of ..
  10. Arai S, Noda Y, Kainuma S, Wada I, Yoda K. Ypt11 functions in bud-directed transport of the Golgi by linking Myo2 to the coatomer subunit Ret2. Curr Biol. 2008;18:987-91 pubmed publisher
    A yeast class V myosin Myo2 transports the Golgi into the bud during its inheritance. However, the mechanism that links the Golgi to Myo2 is unknown...
  11. Liu H, Bretscher A. Characterization of TPM1 disrupted yeast cells indicates an involvement of tropomyosin in directed vesicular transport. J Cell Biol. 1992;118:285-99 pubmed
    ..The phenotype of the TPM1 disrupted cells is very similar to that of a conditional mutation in the MYO2 gene, which encodes a myosin-like protein (Johnston, G. C., J. A. Prendergast, and R. A. Singer. 1991. J. Cell Biol...
  12. Lillie S, Brown S. Suppression of a myosin defect by a kinesin-related gene. Nature. 1992;356:358-61 pubmed
    ..Studies of a temperature-sensitive mutation (myo2-66) in a gene of the myosin superfamily (MYO2) have implicated the Myo2 protein (Myo2p) in the process of polarized ..
  13. Boldogh I, Ramcharan S, Yang H, Pon L. A type V myosin (Myo2p) and a Rab-like G-protein (Ypt11p) are required for retention of newly inherited mitochondria in yeast cells during cell division. Mol Biol Cell. 2004;15:3994-4002 pubmed
    ..We found that deletion of YPT11, reduction of the length of the Myo2p lever arm (myo2-Delta6IQ), or deletion of MYO4 (the other yeast myosin V), had no effect on mitochondrial morphology, ..
  14. Altmann K, Frank M, Neumann D, Jakobs S, Westermann B. The class V myosin motor protein, Myo2, plays a major role in mitochondrial motility in Saccharomyces cerevisiae. J Cell Biol. 2008;181:119-30 pubmed publisher
    ..Here, we establish the class V myosin motor protein, Myo2, as an important mediator of mitochondrial motility in budding yeast...
  15. Hill K, Catlett N, Weisman L. Actin and myosin function in directed vacuole movement during cell division in Saccharomyces cerevisiae. J Cell Biol. 1996;135:1535-49 pubmed
    ..These results present the first demonstration that specific cytoskeletal proteins function in vacuole inheritance. ..
  16. Eves P, Jin Y, Brunner M, Weisman L. Overlap of cargo binding sites on myosin V coordinates the inheritance of diverse cargoes. J Cell Biol. 2012;198:69-85 pubmed publisher
    ..For the yeast vacuole, the myosin V motor, Myo2, and its vacuole-specific cargo adaptor, Vac17, regulate where the vacuole is deposited and the timing of vacuole ..
  17. Govindan B, Bowser R, Novick P. The role of Myo2, a yeast class V myosin, in vesicular transport. J Cell Biol. 1995;128:1055-68 pubmed
    Previous studies have shown that temperature-sensitive, myo2-66 yeast arrest as large, unbudded cells that accumulate vesicles within their cytoplasm (Johnston, G. C., J. A. Prendergast, and R. A. Singer. 1991. J. Cell Biol. 113:539-551)...
  18. Stevens R, Davis T. Mlc1p is a light chain for the unconventional myosin Myo2p in Saccharomyces cerevisiae. J Cell Biol. 1998;142:711-22 pubmed
    ..Surprisingly, we find that precise deletion of the six IQ sites in the neck region results in a myosin, Myo2-Delta6IQp, that can support the growth of a yeast strain at 90% the rate of a wild-type isogenic strain...
  19. Kagami M, Toh e A, Matsui Y. Sro7p, a Saccharomyces cerevisiae counterpart of the tumor suppressor l(2)gl protein, is related to myosins in function. Genetics. 1998;149:1717-27 pubmed
    ..In addition, SRO7 showed genetic interactions with MYO2, encoding an essential type V myosin: Overexpression of SRO7 suppressed a defect in MYO2 and, conversely, ..
  20. Schott D, Ho J, Pruyne D, Bretscher A. The COOH-terminal domain of Myo2p, a yeast myosin V, has a direct role in secretory vesicle targeting. J Cell Biol. 1999;147:791-808 pubmed
    b>MYO2 encodes a type V myosin heavy chain needed for the targeting of vacuoles and secretory vesicles to the growing bud of yeast. Here we describe new myo2 alleles containing conditional lethal mutations in the COOH-terminal tail domain...
  21. Espreafico E, Cheney R, Matteoli M, Nascimento A, De Camilli P, Larson R, et al. Primary structure and cellular localization of chicken brain myosin-V (p190), an unconventional myosin with calmodulin light chains. J Cell Biol. 1992;119:1541-57 pubmed
    ..class of unconventional myosins that includes the gene products encoded by the dilute locus of mouse and the MYO2 gene of Saccharomyces cerevisiae...
  22. Brockerhoff S, Stevens R, Davis T. The unconventional myosin, Myo2p, is a calmodulin target at sites of cell growth in Saccharomyces cerevisiae. J Cell Biol. 1994;124:315-23 pubmed
    ..Second, a genetic analysis indicates that mutations in CMD1 show allele-specific synthetic lethality with the myo2-66 conditional mutation...
  23. Jin Y, Sultana A, Gandhi P, Franklin E, Hamamoto S, Khan A, et al. Myosin V transports secretory vesicles via a Rab GTPase cascade and interaction with the exocyst complex. Dev Cell. 2011;21:1156-70 pubmed publisher
    ..Vesicle production is coupled to transport via direct interaction between Ypt31/32 and the yeast myosin V, Myo2. Here we show that Myo2 interacts directly with Sec4 and the exocyst subunit Sec15...
  24. Förtsch J, Hummel E, Krist M, Westermann B. The myosin-related motor protein Myo2 is an essential mediator of bud-directed mitochondrial movement in yeast. J Cell Biol. 2011;194:473-88 pubmed publisher
    ..It is a matter of debate whether anterograde mitochondrial movement is mediated by the myosin-related motor protein Myo2 or by motor-independent mechanisms...
  25. Johnston G, Prendergast J, Singer R. The Saccharomyces cerevisiae MYO2 gene encodes an essential myosin for vectorial transport of vesicles. J Cell Biol. 1991;113:539-51 pubmed
    ..We show here that this vectorial growth is facilitated by activity of the MYO2 gene...
  26. Fagarasanu A, Fagarasanu M, Eitzen G, Aitchison J, Rachubinski R. The peroxisomal membrane protein Inp2p is the peroxisome-specific receptor for the myosin V motor Myo2p of Saccharomyces cerevisiae. Dev Cell. 2006;10:587-600 pubmed
    ..To our knowledge, Inp2p is the first peroxisomal protein implicated in the vectorial movement of peroxisomes. ..
  27. Pruyne D, Schott D, Bretscher A. Tropomyosin-containing actin cables direct the Myo2p-dependent polarized delivery of secretory vesicles in budding yeast. J Cell Biol. 1998;143:1931-45 pubmed
    ..Therefore, actin cables respond to polarity cues independently of the overall distribution of cortical patches and are able to directly target the Myo2p-dependent delivery of secretory vesicles and polarization of growth. ..
  28. Pashkova N, Catlett N, Novak J, Wu G, Lu R, Cohen R, et al. Myosin V attachment to cargo requires the tight association of two functional subdomains. J Cell Biol. 2005;168:359-64 pubmed
    ..Our results suggest a model whereby intramolecular interactions between the globular tail subdomains help to coordinate the transport of multiple distinct cargoes by myosin V. ..
  29. Itoh T, Toh e A, Matsui Y. Mmr1p is a mitochondrial factor for Myo2p-dependent inheritance of mitochondria in the budding yeast. EMBO J. 2004;23:2520-30 pubmed
    ..We identified MMR1 as a high-dose suppressor of the myo2 mitochondrial defect and that Mmr1p resides restrictively on the bud-localizing mitochondria and forms a complex ..
  30. Lillie S, Brown S. Immunofluorescence localization of the unconventional myosin, Myo2p, and the putative kinesin-related protein, Smy1p, to the same regions of polarized growth in Saccharomyces cerevisiae. J Cell Biol. 1994;125:825-42 pubmed
    b>Myo2 protein (Myo2p), an unconventional myosin in the budding yeast Saccharomyces cerevisiae, has been implicated in polarized growth and secretion by studies of the temperature-sensitive myo2-66 mutant...
  31. Hermann G, King E, Shaw J. The yeast gene, MDM20, is necessary for mitochondrial inheritance and organization of the actin cytoskeleton. J Cell Biol. 1997;137:141-53 pubmed
    ..the same pattern of genetic interactions; mutations in MDM20 are synthetically lethal with mutations in BEM2 and MYO2 but not SAC6...
  32. Rossanese O, Reinke C, Bevis B, Hammond A, Sears I, O CONNOR J, et al. A role for actin, Cdc1p, and Myo2p in the inheritance of late Golgi elements in Saccharomyces cerevisiae. J Cell Biol. 2001;153:47-62 pubmed
    ..We propose that early Golgi elements arise from ER membranes that are present in the bud. These two pathways of Golgi inheritance in S. cerevisiae resemble Golgi inheritance pathways in vertebrate cells. ..
  33. Fagarasanu A, Mast F, Knoblach B, Jin Y, Brunner M, Logan M, et al. Myosin-driven peroxisome partitioning in S. cerevisiae. J Cell Biol. 2009;186:541-54 pubmed publisher
  34. Ishikawa K, Catlett N, Novak J, Tang F, Nau J, Weisman L. Identification of an organelle-specific myosin V receptor. J Cell Biol. 2003;160:887-97 pubmed
    ..Vac17p interacts with the Myo2p cargo-binding domain, but not with vacuole inheritance-defective myo2 mutants that have single amino acid changes within this region...
  35. Robinson N, Guo L, Imai J, Toh e A, Matsui Y, Tamanoi F. Rho3 of Saccharomyces cerevisiae, which regulates the actin cytoskeleton and exocytosis, is a GTPase which interacts with Myo2 and Exo70. Mol Cell Biol. 1999;19:3580-7 pubmed
    ..cerevisiae cDNA library to identify proteins interacting with Rho3. Two proteins, Exo70 and Myo2, were identified in this screen...
  36. Adamo J, Rossi G, Brennwald P. The Rho GTPase Rho3 has a direct role in exocytosis that is distinct from its role in actin polarity. Mol Biol Cell. 1999;10:4121-33 pubmed
    ..We provide evidence that the vesicle delivery function of Rho3 is mediated by the unconventional myosin Myo2 and that the docking and fusion function is mediated by the exocyst component Exo70...
  37. Yau R, Peng Y, Valiathan R, Birkeland S, Wilson T, Weisman L. Release from myosin V via regulated recruitment of an E3 ubiquitin ligase controls organelle localization. Dev Cell. 2014;28:520-33 pubmed publisher
    ..Upon arrival at their correct locations, motors release organelles via unknown mechanisms. The yeast myosin V, Myo2, binds the vacuole-specific adaptor Vac17 to transport the vacuole from the mother cell to the bud...
  38. Ganoth A, Nachliel E, Friedman R, Gutman M. Myosin V movement: lessons from molecular dynamics studies of IQ peptides in the lever arm. Biochemistry. 2007;46:14524-36 pubmed
    ..B., Hodges, A. R., Lu, H., and Trybus, K. M. (2006) J. Biol. Chem. 281, 6079-6086), our observations may account for a general structural feature for the myosins' arm embedded flexibility. ..
  39. Lewandowska A, Macfarlane J, Shaw J. Mitochondrial association, protein phosphorylation, and degradation regulate the availability of the active Rab GTPase Ypt11 for mitochondrial inheritance. Mol Biol Cell. 2013;24:1185-95 pubmed publisher
    The Rab GTPase Ypt11 is a Myo2-binding protein implicated in mother-to-bud transport of the cortical endoplasmic reticulum (ER), late Golgi, and mitochondria during yeast division...
  40. Breton A, Aigle M. Genetic and functional relationship between Rvsp, myosin and actin in Saccharomyces cerevisiae. Curr Genet. 1998;34:280-6 pubmed
    ..by the Rvsp proteins and actin is strengthened further by the fact that the double mutants rvs167, myo1 and rvs167, myo2 are lethal and severely affected in growth respectively...
  41. Wesche S, Arnold M, Jansen R. The UCS domain protein She4p binds to myosin motor domains and is essential for class I and class V myosin function. Curr Biol. 2003;13:715-24 pubmed
    ..This localization requires Myo2p and actin, suggesting a Myo2-dependent mechanism by which She4p is targeted to the bud cap...
  42. Panni S, Montecchi Palazzi L, Kiemer L, Cabibbo A, Paoluzi S, Santonico E, et al. Combining peptide recognition specificity and context information for the prediction of the 14-3-3-mediated interactome in S. cerevisiae and H. sapiens. Proteomics. 2011;11:128-43 pubmed publisher
    ..Our approach provides an orthogonal reliability assessment and maps with high confidence the 14-3-3 peptide target on the partner proteins. ..
  43. Gandhi M, Jangi M, Goode B. Functional surfaces on the actin-binding protein coronin revealed by systematic mutagenesis. J Biol Chem. 2010;285:34899-908 pubmed publisher
    ..Conservation of the actin-binding surfaces across distant species and in all three major classes of coronin isoforms suggests that the nature of the coronin-actin association may be similar in other family members. ..
  44. Toi H, Fujimura Kamada K, Irie K, Takai Y, Todo S, Tanaka K. She4p/Dim1p interacts with the motor domain of unconventional myosins in the budding yeast, Saccharomyces cerevisiae. Mol Biol Cell. 2003;14:2237-49 pubmed
    ..Our results suggest that She4p/Dim1p is required for structural integrity or regulation of the motor domain of unconventional myosins. ..
  45. Sheltzer J, Rose M. The class V myosin Myo2p is required for Fus2p transport and actin polarization during the yeast mating response. Mol Biol Cell. 2009;20:2909-19 pubmed publisher
    ..The different velocities, differential sensitivity to mutation and lack of colocalization suggest that Fus2p and Sec4p, another Myo2p cargo associated with exocytotic vesicles, reside predominantly on different cellular organelles. ..
  46. Kadota J, Yamamoto T, Yoshiuchi S, Bi E, Tanaka K. Septin ring assembly requires concerted action of polarisome components, a PAK kinase Cla4p, and the actin cytoskeleton in Saccharomyces cerevisiae. Mol Biol Cell. 2004;15:5329-45 pubmed
    ..Our results suggest that polarisome components and Cla4p are required for the initial assembly of the septin ring and that the actin cytoskeleton is involved in this process. ..
  47. Casavola E, Catucci A, Bielli P, Di Pentima A, Porcu G, Pennestri M, et al. Ypt32p and Mlc1p bind within the vesicle binding region of the class V myosin Myo2p globular tail domain. Mol Microbiol. 2008;67:1051-66 pubmed publisher
    ..Our data are consistent with a role of Ypt32p and Mlc1p in regulating the interaction of post-Golgi carriers with Myo2p subdomain II. ..
  48. Demmel L, Beck M, Klose C, Schlaitz A, Gloor Y, Hsu P, et al. Nucleocytoplasmic shuttling of the Golgi phosphatidylinositol 4-kinase Pik1 is regulated by 14-3-3 proteins and coordinates Golgi function with cell growth. Mol Biol Cell. 2008;19:1046-61 pubmed publisher
    ..These data suggest a role of Pik1p nucleocytoplasmic shuttling in coordination of biosynthetic transport from the Golgi with nutrient signaling. ..
  49. Rossi G, Brennwald P. Yeast homologues of lethal giant larvae and type V myosin cooperate in the regulation of Rab-dependent vesicle clustering and polarized exocytosis. Mol Biol Cell. 2011;22:842-57 pubmed publisher
    ..In this article, we characterize the interaction between Sro7 and the type V myosin Myo2 and show that this interaction is important for two distinct aspects of Sro7 function...
  50. Shih J, Reck Peterson S, Newitt R, Mooseker M, Aebersold R, Herskowitz I. Cell polarity protein Spa2P associates with proteins involved in actin function in Saccharomyces cerevisiae. Mol Biol Cell. 2005;16:4595-608 pubmed
    ..In addition, we observe an enhanced cell-separation defect in a myo1spa2 strain at 37 degrees C. This provides further evidence that Spa2p is involved in cytokinesis and cell wall morphogenesis. ..
  51. Cuschieri L, Miller R, Vogel J. Gamma-tubulin is required for proper recruitment and assembly of Kar9-Bim1 complexes in budding yeast. Mol Biol Cell. 2006;17:4420-34 pubmed
    ..Moreover, Myo2- and F-actin-dependent dwelling of Kar9 in the bud is observed in tub4-delta dsyl cells, suggesting defective Kar9 ..
  52. Steffens A, Jaegle B, Tresch A, Hulskamp M, Jakoby M. Processing-body movement in Arabidopsis depends on an interaction between myosins and DECAPPING PROTEIN1. Plant Physiol. 2014;164:1879-92 pubmed publisher
    ..Interspecies and intraspecies interaction approaches with mammalian and yeast protein homologs suggest that this mechanism is evolutionarily conserved among eukaryotes. ..
  53. Chang W, Zaarour R, Reck Peterson S, Rinn J, Singer R, Snyder M, et al. Myo2p, a class V myosin in budding yeast, associates with a large ribonucleic acid-protein complex that contains mRNAs and subunits of the RNA-processing body. RNA. 2008;14:491-502 pubmed publisher
    ..P-body disassembly is delayed in the motor mutant, myo2-66, indicating that Myo2p may facilitate the release of mRNAs from the P-body.
  54. Freedman T, Porter A, Haarer B. Mutational and hyperexpression-induced disruption of bipolar budding in yeast. Microbiology. 2000;146 ( Pt 11):2833-43 pubmed
    ..A mutant allele of the MYO2 gene, encoding a class-V unconventional myosin was identified that perturbs bipolar budding without affecting axial ..
  55. Otzen M, Rucktäschel R, Thoms S, Emmrich K, Krikken A, Erdmann R, et al. Pex19p contributes to peroxisome inheritance in the association of peroxisomes to Myo2p. Traffic. 2012;13:947-59 pubmed publisher
    ..Together these data suggest that both Inp2p and Pex19p are required for proper association of peroxisomes to Myo2p...
  56. Finger F, Novick P. Synthetic interactions of the post-Golgi sec mutations of Saccharomyces cerevisiae. Genetics. 2000;156:943-51 pubmed
    ..The significance of these results is discussed in the context of both secretory pathway function and the utility of synthetic lethality studies and their interpretation. ..
  57. Tomala K, Korona R. Alleviation of deleterious effects of protein mutation through inactivation of molecular chaperones. Mol Genet Genomics. 2008;280:409-17 pubmed publisher
    ..We suggest that under non-stressful conditions, molecular chaperones are tuned to support folding of native proteins, but not that of mutated ones. ..
  58. Terrak M, Otterbein L, Wu G, Palecanda L, Lu R, Dominguez R. Crystallization, X-ray characterization and selenomethionine phasing of Mlc1p bound to IQ motifs from myosin V. Acta Crystallogr D Biol Crystallogr. 2002;58:1882-5 pubmed
    ..The other two structures (Mlc1p-IQ2 and Mlc1p-IQ3) were determined by molecular replacement using the partially refined structure of Mlc1p-IQ2,3 as a search model. ..
  59. Makanae K, Kintaka R, Makino T, Kitano H, Moriya H. Identification of dosage-sensitive genes in Saccharomyces cerevisiae using the genetic tug-of-war method. Genome Res. 2013;23:300-11 pubmed publisher
    ..The results obtained in this study will provide basic knowledge about the physiology of chromosomal abnormalities and the evolution of chromosomal composition. ..
  60. Hodges A, Krementsova E, Bookwalter C, Fagnant P, Sladewski T, Trybus K. Tropomyosin is essential for processive movement of a class V myosin from budding yeast. Curr Biol. 2012;22:1410-6 pubmed publisher
    ..Tropomyosin slows the rate of MgADP release, thus increasing the time the motor spends strongly attached to actin. This is the first example of tropomyosin switching a motor from nonprocessive to processive motion on actin. ..
  61. Mazzoni C, Zarov P, Rambourg A, Mann C. The SLT2 (MPK1) MAP kinase homolog is involved in polarized cell growth in Saccharomyces cerevisiae. J Cell Biol. 1993;123:1821-33 pubmed
    ..Furthermore, slt2::HIS3 act1-1 and slt2::HIS3 myo2-66 double mutants are inviable...
  62. Dunn B, Sakamoto T, Hong M, Sellers J, Takizawa P. Myo4p is a monomeric myosin with motility uniquely adapted to transport mRNA. J Cell Biol. 2007;178:1193-206 pubmed
    ..Our results suggest that the oligomeric state of Myo4p is important for its motility and ability to transport mRNA. ..
  63. Yakir Tamang L, Gerst J. A phosphatidylinositol-transfer protein and phosphatidylinositol-4-phosphate 5-kinase control Cdc42 to regulate the actin cytoskeleton and secretory pathway in yeast. Mol Biol Cell. 2009;20:3583-97 pubmed publisher
    ..Similar defects were observed in sec, myo2-66, and cdc42-6 mutants at elevated temperatures and were rescued by the overexpression of MSS4...
  64. Alfaro G, Johansen J, Dighe S, Duamel G, Kozminski K, Beh C. The sterol-binding protein Kes1/Osh4p is a regulator of polarized exocytosis. Traffic. 2011;12:1521-36 pubmed publisher
    ..These results support a model in which Osh4p acts as a sterol-dependent regulator of polarized vesicle transport, as opposed to being a sterol-transfer protein. ..
  65. Pennestri M, Melino S, Contessa G, Casavola E, Paci M, Ragnini Wilson A, et al. Structural basis for the interaction of the myosin light chain Mlc1p with the myosin V Myo2p IQ motifs. J Biol Chem. 2007;282:667-79 pubmed
    ..This finding gives us a novel view of how calmodulin and essential light chain, through a differential binding to IQ1 of class V myosin motor, regulate this activity during vegetative growth and cytokinesis. ..
  66. Chang J, Mast F, Fagarasanu A, Rachubinski D, Eitzen G, Dacks J, et al. Pex3 peroxisome biogenesis proteins function in peroxisome inheritance as class V myosin receptors. J Cell Biol. 2009;187:233-46 pubmed publisher
    ..Our results point to a temporal link between peroxisome formation and inheritance and delineate a general mechanism of peroxisome inheritance in eukaryotic cells. ..