MYO1

Summary

Gene Symbol: MYO1
Description: myosin 1
Alias: myosin 1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Schmidt M, Bowers B, Varma A, Roh D, Cabib E. In budding yeast, contraction of the actomyosin ring and formation of the primary septum at cytokinesis depend on each other. J Cell Sci. 2002;115:293-302 pubmed
    Saccharomyces cerevisiae chs2 mutants are unable to synthesize primary septum chitin, and myo1 mutants cannot construct a functional contractile ring. The morphology of the two mutants, as observed by electron microscopy, is very similar...
  2. Wang T, Bretscher A. The rho-GAP encoded by BEM2 regulates cytoskeletal structure in budding yeast. Mol Biol Cell. 1995;6:1011-24 pubmed
    ..bem2 mutations also show synthetic lethality with rho1 and mutations in certain other cytoskeletal genes (ACT1, MYO1, MYO2, and SAC6) but not with mutations in several noncytoskeletal genes...
  3. Ko N, Nishihama R, Tully G, Ostapenko D, Solomon M, Morgan D, et al. Identification of yeast IQGAP (Iqg1p) as an anaphase-promoting-complex substrate and its role in actomyosin-ring-independent cytokinesis. Mol Biol Cell. 2007;18:5139-53 pubmed
    ..Deletion of MYO1 (the only myosin II gene) is lethal on rich medium in the W303 strain background and causes slow-growth and delayed-..
  4. Lord M, Laves E, Pollard T. Cytokinesis depends on the motor domains of myosin-II in fission yeast but not in budding yeast. Mol Biol Cell. 2005;16:5346-55 pubmed
    ..Myo1p is the first example of a myosin whose cellular function does not require a catalytic motor domain revealing a novel mechanism of action for budding yeast myosin-II independent of actin binding and ATPase activity. ..
  5. Jendretzki A, Ciklic I, Rodicio R, Schmitz H, Heinisch J. Cyk3 acts in actomyosin ring independent cytokinesis by recruiting Inn1 to the yeast bud neck. Mol Genet Genomics. 2009;282:437-51 pubmed publisher
    ..Moreover, overexpression of CYK3 in a myo1 or an iqg1 deletion not only restores viability, but also the recruitment of Inn1 to the bud neck...
  6. Watts F, Shiels G, Orr E. The yeast MYO1 gene encoding a myosin-like protein required for cell division. EMBO J. 1987;6:3499-505 pubmed
    A yeast gene MYO1 that contains regions of substantial sequence homology with the nematode muscle myosin gene (unc54) has been isolated and sequenced...
  7. Meitinger F, Boehm M, Hofmann A, Hub B, Zentgraf H, Lehmann W, et al. Phosphorylation-dependent regulation of the F-BAR protein Hof1 during cytokinesis. Genes Dev. 2011;25:875-88 pubmed publisher
    ..Genetic and functional data support a model in which Dbf2-Mob1 regulates Hof1 by inducing domain rearrangements, leading to the exposure of the Hof1 RLS domain during telophase. ..
  8. Nishihama R, Schreiter J, Onishi M, Vallen E, Hanna J, Moravcevic K, et al. Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae. J Cell Biol. 2009;185:995-1012 pubmed publisher
    ..Thus, Inn1 and Cyk3 appear to cooperate in activating Chs2 for PS formation, which allows coordination of AMR contraction with ingression of the cleavage furrow. ..
  9. Wloka C, Nishihama R, Onishi M, Oh Y, Hanna J, Pringle J, et al. Evidence that a septin diffusion barrier is dispensable for cytokinesis in budding yeast. Biol Chem. 2011;392:813-29 pubmed publisher

More Information

Publications50

  1. Bi E, Maddox P, Lew D, Salmon E, McMillan J, Yeh E, et al. Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis. J Cell Biol. 1998;142:1301-12 pubmed
    ..Strains deleted for MYO1 are viable; they fail to form the actin ring but show apparently normal congregation of actin patches at the neck...
  2. Vallen E, Caviston J, Bi E. Roles of Hof1p, Bni1p, Bnr1p, and myo1p in cytokinesis in Saccharomyces cerevisiae. Mol Biol Cell. 2000;11:593-611 pubmed
    ..In bni1Delta cells that lack the actomyosin contraction, septum formation is often slow and asymmetric, suggesting that actomyosin contraction may provide directionality for efficient septum formation. ..
  3. Luo J, Vallen E, Dravis C, Tcheperegine S, Drees B, Bi E. Identification and functional analysis of the essential and regulatory light chains of the only type II myosin Myo1p in Saccharomyces cerevisiae. J Cell Biol. 2004;165:843-55 pubmed
    ..We also identify Mlc2p as the regulatory light chain for Myo1p and demonstrate its role in Myo1p ring disassembly, a function likely conserved among eukaryotes. ..
  4. Meitinger F, Petrova B, Lombardi I, Bertazzi D, Hub B, Zentgraf H, et al. Targeted localization of Inn1, Cyk3 and Chs2 by the mitotic-exit network regulates cytokinesis in budding yeast. J Cell Sci. 2010;123:1851-61 pubmed publisher
    ..This defect was partially suppressed by increased levels of Cyk3. We therefore propose that the MEN directly controls cytokinesis via targeting of Inn1, Cyk3 and Chs2 to the bud neck...
  5. Rivera Ruiz M, Rodríguez Quiñones J, Akamine P, RODRIGUEZ MEDINA J. Post-transcriptional regulation in the myo1? mutant of Saccharomyces cerevisiae. BMC Genomics. 2010;11:690 pubmed publisher
    Saccharomyces cerevisiae myosin type II-deficient (myo1?) strains remain viable and divide, despite the absence of a cytokinetic ring, by activation of the PKC1-dependent cell wall integrity pathway (CWIP)...
  6. Díaz Blanco N, RODRIGUEZ MEDINA J. Dosage rescue by UBC4 restores cell wall integrity in Saccharomyces cerevisiae lacking the myosin type II gene MYO1. Yeast. 2007;24:343-55 pubmed
    Myosin II is important for normal cytokinesis and cell wall maintenance in yeast cells. Myosin II-deficient (myo1) strains of the budding yeast Saccharomyces cerevisiae are hypersensitive to nikkomycin Z (NZ), a competitive inhibitor of ..
  7. Boyne J, Yosuf H, Bieganowski P, Brenner C, Price C. Yeast myosin light chain, Mlc1p, interacts with both IQGAP and class II myosin to effect cytokinesis. J Cell Sci. 2000;113 Pt 24:4533-43 pubmed
    ..It is proposed that Mlc1p therefore functions to recruit Iqg1p and in turn actin to the actomyosin ring and that it is also required for Myo1p function during ring contraction. ..
  8. Rodríguez Quiñones J, Irizarry R, Díaz Blanco N, Rivera Molina F, Gómez Garzón D, RODRIGUEZ MEDINA J. Global mRNA expression analysis in myosin II deficient strains of Saccharomyces cerevisiae reveals an impairment of cell integrity functions. BMC Genomics. 2008;9:34 pubmed publisher
    The Saccharomyces cerevisiae MYO1 gene encodes the myosin II heavy chain (Myo1p), a protein required for normal cytokinesis in budding yeast...
  9. Lippincott J, Li R. Sequential assembly of myosin II, an IQGAP-like protein, and filamentous actin to a ring structure involved in budding yeast cytokinesis. J Cell Biol. 1998;140:355-66 pubmed
  10. Korinek W, Bi E, Epp J, Wang L, Ho J, Chant J. Cyk3, a novel SH3-domain protein, affects cytokinesis in yeast. Curr Biol. 2000;10:947-50 pubmed
    ..Thus, Cyk3 appears to be important for cytokinesis and acts potentially downstream of Iqg1. ..
  11. Fang X, Luo J, Nishihama R, Wloka C, Dravis C, Travaglia M, et al. Biphasic targeting and cleavage furrow ingression directed by the tail of a myosin II. J Cell Biol. 2010;191:1333-50 pubmed publisher
    ..Here we show that Myo1 is a two-headed myosin II in Saccharomyces cerevisiae, and that Myo1 localizes to the division site via two ..
  12. Wagner W, Bielli P, Wacha S, Ragnini Wilson A. Mlc1p promotes septum closure during cytokinesis via the IQ motifs of the vesicle motor Myo2p. EMBO J. 2002;21:6397-408 pubmed
    ..Further more, myosin light chains, via their ability to be transported by secretory vesicles and to interact with class V myosin IQ motifs, can regulate vesicle polarization processes at a specific location and stage of the cell cycle. ..
  13. Yoshida S, Bartolini S, Pellman D. Mechanisms for concentrating Rho1 during cytokinesis. Genes Dev. 2009;23:810-23 pubmed publisher
    ..These findings comprehensively define the targeting mechanisms of Rho1 essential for cytokinesis in yeast, and are likely to be relevant to cytokinesis in other organisms. ..
  14. Tolliday N, Pitcher M, Li R. Direct evidence for a critical role of myosin II in budding yeast cytokinesis and the evolvability of new cytokinetic mechanisms in the absence of myosin II. Mol Biol Cell. 2003;14:798-809 pubmed
    ..Here we show that deletion of MYO1, the single myosin II gene, is lethal in a commonly used strain background...
  15. Stark B, Wen K, Allingham J, Rubenstein P, Lord M. Functional adaptation between yeast actin and its cognate myosin motors. J Biol Chem. 2011;286:30384-92 pubmed publisher
    ..Our study also highlights the importance of native actin isoforms when considering the function of myosins. ..
  16. Pagán Mercado G, Santiago Cartagena E, Akamine P, RODRIGUEZ MEDINA J. Functional and genetic interactions of TOR in the budding yeast Saccharomyces cerevisiae with myosin type II-deficiency (myo1?). BMC Cell Biol. 2012;13:13 pubmed publisher
    Yeast has numerous mechanisms to survive stress. Deletion of myosin type II (myo1?) in Saccharomyces cerevisiae results in a cell that has defective cytokinesis...
  17. Makanae K, Kintaka R, Makino T, Kitano H, Moriya H. Identification of dosage-sensitive genes in Saccharomyces cerevisiae using the genetic tug-of-war method. Genome Res. 2013;23:300-11 pubmed publisher
    ..The results obtained in this study will provide basic knowledge about the physiology of chromosomal abnormalities and the evolution of chromosomal composition. ..
  18. Roelants F, Su B, von Wulffen J, Ramachandran S, Sartorel E, TROTT A, et al. Protein kinase Gin4 negatively regulates flippase function and controls plasma membrane asymmetry. J Cell Biol. 2015;208:299-311 pubmed publisher
  19. Atkins B, Yoshida S, Saito K, Wu C, Lew D, Pellman D. Inhibition of Cdc42 during mitotic exit is required for cytokinesis. J Cell Biol. 2013;202:231-40 pubmed publisher
    ..The effects of Cdc42 hyperactivation are largely mediated by the Cdc42 effector p21-activated kinase Ste20. Inhibition of Cdc42 and related Rho guanosine triphosphatases may be a general feature of cytokinesis in eukaryotes. ..
  20. Gao L, Bretscher A. Polarized growth in budding yeast in the absence of a localized formin. Mol Biol Cell. 2009;20:2540-8 pubmed publisher
    ..Our results show that multiple mechanisms contribute to cable orientation and polarized growth, with localized formins and myosin II being two major contributors. ..
  21. Feng L, Kim E, Lee W, Miller C, Kuang B, Reisler E, et al. Fluorescence probing of yeast actin subdomain 3/4 hydrophobic loop 262-274. Actin-actin and actin-myosin interactions in actin filaments. J Biol Chem. 1997;272:16829-37 pubmed
    ..These results help establish the orientation of monomers in F-actin and show that the binding of S1 to actin subdomains 1 and 2 affects the environment of the loop between subdomains 3 and 4. ..
  22. Palani S, Meitinger F, Boehm M, Lehmann W, Pereira G. Cdc14-dependent dephosphorylation of Inn1 contributes to Inn1-Cyk3 complex formation. J Cell Sci. 2012;125:3091-6 pubmed publisher
    ..We propose that Cdc14 counteracts Cdk1 phosphorylation of Inn1 to facilitate Inn1-Cyk3 complex formation and so promote cytokinesis. ..
  23. Oh Y, Schreiter J, Nishihama R, Wloka C, Bi E. Targeting and functional mechanisms of the cytokinesis-related F-BAR protein Hof1 during the cell cycle. Mol Biol Cell. 2013;24:1305-20 pubmed publisher
    ..In contrast, the C-terminal half of Hof1 interacts with Myo1, the sole myosin-II heavy chain in budding yeast, and localizes to the bud neck in a Myo1-dependent manner from the ..
  24. Shih J, Reck Peterson S, Newitt R, Mooseker M, Aebersold R, Herskowitz I. Cell polarity protein Spa2P associates with proteins involved in actin function in Saccharomyces cerevisiae. Mol Biol Cell. 2005;16:4595-608 pubmed
    ..In addition, we observe an enhanced cell-separation defect in a myo1spa2 strain at 37 degrees C. This provides further evidence that Spa2p is involved in cytokinesis and cell wall morphogenesis. ..
  25. Mancini Lombardi I, Palani S, Meitinger F, Darieva Z, Hofmann A, Sharrocks A, et al. Lre1 directly inhibits the NDR/Lats kinase Cbk1 at the cell division site in a phosphorylation-dependent manner. Curr Biol. 2013;23:1736-45 pubmed publisher
    ..We propose that similar inhibitory proteins may also provide fine tuning for the activity of NDR kinases in other organisms. ..
  26. Sirotkin V, Beltzner C, Marchand J, Pollard T. Interactions of WASp, myosin-I, and verprolin with Arp2/3 complex during actin patch assembly in fission yeast. J Cell Biol. 2005;170:637-48 pubmed
    ..Genetic dependencies of patch formation suggest that patch formation involves cross talk between Myo1p and Wsp1p/Vrp1p pathways. ..
  27. Lillie S, Brown S. Smy1p, a kinesin-related protein that does not require microtubules. J Cell Biol. 1998;140:873-83 pubmed
    ..We conclude that Smy1p does not act as a microtubule-based motor to localize properly or to compensate for defective Myo2p, but that it must instead act in some novel way. ..
  28. Grosshans B, Grötsch H, Mukhopadhyay D, Fernández I, Pfannstiel J, Idrissi F, et al. TEDS site phosphorylation of the yeast myosins I is required for ligand-induced but not for constitutive endocytosis of the G protein-coupled receptor Ste2p. J Biol Chem. 2006;281:11104-14 pubmed
  29. May K, Win T, Hyams J. Yeast myosin II: a new subclass of unconventional conventional myosins?. Cell Motil Cytoskeleton. 1998;39:195-200 pubmed
    ..On the basis of these findings, we propose that the yeast myosin IIs constitute a divergent fifth class of "unconventional" conventional myosins. ..
  30. Rancati G, Pavelka N, Fleharty B, Noll A, Trimble R, Walton K, et al. Aneuploidy underlies rapid adaptive evolution of yeast cells deprived of a conserved cytokinesis motor. Cell. 2008;135:879-93 pubmed publisher
    ..We show that yeast cells deleted of MYO1, encoding the only myosin II normally required for cytokinesis, rapidly evolved divergent pathways to restore ..
  31. Toi H, Fujimura Kamada K, Irie K, Takai Y, Todo S, Tanaka K. She4p/Dim1p interacts with the motor domain of unconventional myosins in the budding yeast, Saccharomyces cerevisiae. Mol Biol Cell. 2003;14:2237-49 pubmed
    ..Our results suggest that She4p/Dim1p is required for structural integrity or regulation of the motor domain of unconventional myosins. ..
  32. Kagami M, Toh e A, Matsui Y. Sro7p, a Saccharomyces cerevisiae counterpart of the tumor suppressor l(2)gl protein, is related to myosins in function. Genetics. 1998;149:1717-27 pubmed
    ..Our genetic analysis revealed that deletion of SRO7 and SRO77 showed reciprocal suppression with deletion of MYO1 (i.e., the sro7Delta sro77Delta defect was suppressed by myo1Delta and vice versa)...
  33. Song S, Lee K. A novel function of Saccharomyces cerevisiae CDC5 in cytokinesis. J Cell Biol. 2001;152:451-69 pubmed
    ..and septins (Cdc11 and Cdc12) and genetic interactions between the dominant-negative cdc5DeltaN and Cyk2/Hof1 or Myo1 suggest that direct interactions between cdc5DeltaN and septins resulted in inhibition of Cyk2/Hof1- and Myo1-..
  34. Lewellyn E, Pedersen R, Hong J, Lu R, Morrison H, Drubin D. An Engineered Minimal WASP-Myosin Fusion Protein Reveals Essential Functions for Endocytosis. Dev Cell. 2015;35:281-94 pubmed publisher
    ..The requirement for both membrane and F-actin binding reveals that myosin-mediated coupling between actin filaments and the base of endocytic sites is essential for allowing actin polymerization to drive membrane invagination. ..
  35. Roumanie O, Peypouquet M, Bonneu M, Thoraval D, Doignon F, Crouzet M. Evidence for the genetic interaction between the actin-binding protein Vrp1 and the RhoGAP Rgd1 mediated through Rho3p and Rho4p in Saccharomyces cerevisiae. Mol Microbiol. 2000;36:1403-14 pubmed
    ..This protein structure might act with myosins in various actin cytoskeleton-based activities, in co-operation with a Rho3p/Rho4p signalling pathway that is negatively regulated by Rgd1p GAP activity. ..
  36. Tian C, Wu Y, Johnsson N. Stepwise and cooperative assembly of a cytokinetic core complex in Saccharomyces cerevisiae. J Cell Sci. 2014;127:3614-24 pubmed publisher
    ..We propose that the MIH complex acts as hinge between the AMR and the proteins involved in cell wall synthesis and membrane attachment. ..
  37. Breton A, Aigle M. Genetic and functional relationship between Rvsp, myosin and actin in Saccharomyces cerevisiae. Curr Genet. 1998;34:280-6 pubmed
    ..fulfilled both by the Rvsp proteins and actin is strengthened further by the fact that the double mutants rvs167, myo1 and rvs167, myo2 are lethal and severely affected in growth respectively...
  38. Ishiwata Kimata Y, Yamamoto Y, Takizawa K, Kohno K, Kimata Y. F-actin and a type-II myosin are required for efficient clustering of the ER stress sensor Ire1. Cell Struct Funct. 2013;38:135-43 pubmed
    ..Ire1 cluster formation was also impaired by deletion of the type-II myosin gene MYO1 or SAC6, which encodes the actin-bundling protein fimbrin...
  39. Feng Z, Okada S, Cai G, Zhou B, Bi E. Myosin‑II heavy chain and formin mediate the targeting of myosin essential light chain to the division site before and during cytokinesis. Mol Biol Cell. 2015;26:1211-24 pubmed publisher
    MLC1 is a haploinsufficient gene encoding the essential light chain for Myo1, the sole myosin‑II heavy chain in the budding yeast Saccharomyces cerevisiae...
  40. Roumanie O, Peypouquet M, Thoraval D, Doignon F, Crouzet M. Functional interactions between the VRP1-LAS17 and RHO3-RHO4 genes involved in actin cytoskeleton organization in Saccharomyces cerevisiae. Curr Genet. 2002;40:317-25 pubmed
    ..In addition, other observations lead us to propose that Rvs167p may act as a linking protein between the two cellular elements. ..
  41. Foltman M, Molist I, Arcones I, Sacristan C, Filali Mouncef Y, Roncero C, et al. Ingression Progression Complexes Control Extracellular Matrix Remodelling during Cytokinesis in Budding Yeast. PLoS Genet. 2016;12:e1005864 pubmed publisher
    ..These findings support a model for the co-ordinated regulation of cell division in budding yeast, in which IPCs play a central role. ..