MUS81

Summary

Gene Symbol: MUS81
Description: Mus81p
Alias: SLX3, Mus81p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Kang M, Lee C, Kang Y, Cho I, Nguyen T, Seo Y. Genetic and functional interactions between Mus81-Mms4 and Rad27. Nucleic Acids Res. 2010;38:7611-25 pubmed publisher
    ..b>Mus81-Mms4 is a structure-specific endonuclease that can resolve stalled replication forks as well as toxic ..
  2. Gritenaite D, Princz L, Szakal B, Bantele S, Wendeler L, Schilbach S, et al. A cell cycle-regulated Slx4-Dpb11 complex promotes the resolution of DNA repair intermediates linked to stalled replication. Genes Dev. 2014;28:1604-19 pubmed publisher
    ..Here, we identify a regulatory mechanism in yeast that critically controls JM resolution by the Mus81-Mms4 endonuclease...
  3. Interthal H, Heyer W. MUS81 encodes a novel helix-hairpin-helix protein involved in the response to UV- and methylation-induced DNA damage in Saccharomyces cerevisiae. Mol Gen Genet. 2000;263:812-27 pubmed
    ..b>Mus81p also shares homology with motifs found in the XPF endonuclease superfamily...
  4. Fabre F, Chan A, Heyer W, Gangloff S. Alternate pathways involving Sgs1/Top3, Mus81/ Mms4, and Srs2 prevent formation of toxic recombination intermediates from single-stranded gaps created by DNA replication. Proc Natl Acad Sci U S A. 2002;99:16887-92 pubmed
    ..Here, we identify a similar interaction involving double mutations affecting Sgs1 or Top3 and Mus81 or Mms4...
  5. Coté A, Lewis S. Mus81-dependent double-strand DNA breaks at in vivo-generated cruciform structures in S. cerevisiae. Mol Cell. 2008;31:800-12 pubmed publisher
    ..In vivo resolution requires Mus81, for which the bacterial HJ resolvase RusA will substitute...
  6. Fu Y, Xiao W. Functional domains required for the Saccharomyces cerevisiae Mus81-Mms4 endonuclease complex formation and nuclear localization. DNA Repair (Amst). 2003;2:1435-47 pubmed
    The Saccharomyces cerevisiae Mms4 and Mus81 proteins form a specific complex, which functions as an endonuclease specific for branched DNA molecules and protects cells from killing by DNA alkylation damage, but not damage induced by ..
  7. Jessop L, Lichten M. Mus81/Mms4 endonuclease and Sgs1 helicase collaborate to ensure proper recombination intermediate metabolism during meiosis. Mol Cell. 2008;31:313-23 pubmed publisher
    Budding yeast lacking the Sgs1 helicase and the Mus81/Mms4 endonuclease are inviable, and indirect studies implicate homologous recombination gone awry as the cause of death...
  8. Mullen J, Kaliraman V, Ibrahim S, Brill S. Requirement for three novel protein complexes in the absence of the Sgs1 DNA helicase in Saccharomyces cerevisiae. Genetics. 2001;157:103-18 pubmed
    ..Mutations in MMS4 (SLX2) and SLX3 generate identical phenotypes, including weak UV and strong MMS hypersensitivity, complete loss of sporulation, and ..
  9. Ehmsen K, Heyer W. Saccharomyces cerevisiae Mus81-Mms4 is a catalytic, DNA structure-selective endonuclease. Nucleic Acids Res. 2008;36:2182-95 pubmed publisher
    The DNA structure-selective endonuclease Mus81-Mms4/Eme1 is a context-specific recombination factor that supports DNA replication, but is not essential for DSB repair in Saccharomyces cerevisiae. We overexpressed Mus81-Mms4 in S...

More Information

Publications67

  1. Ii M, Ii T, Brill S. Mus81 functions in the quality control of replication forks at the rDNA and is involved in the maintenance of rDNA repeat number in Saccharomyces cerevisiae. Mutat Res. 2007;625:1-19 pubmed
    Previous studies in yeast have suggested that the SGS1 DNA helicase or the Mus81-Mms4 structure-specific endonuclease is required to suppress the accumulation of lethal recombination intermediates during DNA replication...
  2. Ii M, Brill S. Roles of SGS1, MUS81, and RAD51 in the repair of lagging-strand replication defects in Saccharomyces cerevisiae. Curr Genet. 2005;48:213-25 pubmed
    Yeast cells lacking the SGS1 DNA helicase and the MUS81 structure-specific endonuclease display a synthetic lethality that is suppressed by loss of the RAD51 recombinase...
  3. Szakal B, Branzei D. Premature Cdk1/Cdc5/Mus81 pathway activation induces aberrant replication and deleterious crossover. EMBO J. 2013;32:1155-67 pubmed publisher
    ..a G2/M damage checkpoint-independent, but Cdk1/Cdc5-dependent pathway that proceeds via a previously described Mus81-Mms4-activating phosphorylation...
  4. Mazon G, Symington L. Mph1 and Mus81-Mms4 prevent aberrant processing of mitotic recombination intermediates. Mol Cell. 2013;52:63-74 pubmed publisher
    ..Saccharomyces cerevisiae Mph1 helicase prevents crossovers between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10 cleavage. A role for Yen1 is only apparent in the absence of Mus81...
  5. Hwang J, Smith S, Myung K. The Rad1-Rad10 complex promotes the production of gross chromosomal rearrangements from spontaneous DNA damage in Saccharomyces cerevisiae. Genetics. 2005;169:1927-37 pubmed
    ..Results presented here suggest that Rad1-Rad10 functions at different stages of GCR formation and that there is an alternative pathway for the GCR formation that is independent of Rad1-Rad10. ..
  6. Oh S, Lao J, Taylor A, Smith G, Hunter N. RecQ helicase, Sgs1, and XPF family endonuclease, Mus81-Mms4, resolve aberrant joint molecules during meiotic recombination. Mol Cell. 2008;31:324-36 pubmed publisher
    Saccharomyces cerevisiae RecQ helicase, Sgs1, and XPF family endonuclease, Mus81-Mms4, are implicated in processing joint molecule (JM) recombination intermediates...
  7. Ashton T, Mankouri H, Heidenblut A, McHugh P, Hickson I. Pathways for Holliday junction processing during homologous recombination in Saccharomyces cerevisiae. Mol Cell Biol. 2011;31:1921-33 pubmed publisher
    ..Deletion of SGS1, TOP3, or RMI1 is synthetically lethal when combined with the loss of the Mus81-Mms4 or Slx1-Slx4 endonucleases, which have been implicated in Holliday junction (HJ) resolution...
  8. Tay Y, Wu L. Overlapping roles for Yen1 and Mus81 in cellular Holliday junction processing. J Biol Chem. 2010;285:11427-32 pubmed publisher
    ..We found that Yen1 acts redundantly with Mus81, but not Slx1, to resolve a model HJ in vivo...
  9. Mullen J, Nallaseth F, Lan Y, Slagle C, Brill S. Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex. Mol Cell Biol. 2005;25:4476-87 pubmed
    ..in the SGS1-TOP3 pathway, based on the observation that strains lacking any one of these genes require MUS81 and MMS4 for viability...
  10. Blanco M, Matos J, Rass U, Ip S, West S. Functional overlap between the structure-specific nucleases Yen1 and Mus81-Mms4 for DNA-damage repair in S. cerevisiae. DNA Repair (Amst). 2010;9:394-402 pubmed publisher
    ..is dependent upon RAD52, indicating that toxic recombination intermediates accumulate in the absence of Yen1 and Mus81. After MMS treatment, yen1Delta mus81Delta double mutants arrest with a G2 DNA content and unsegregated ..
  11. Matos J, Blanco M, Maslen S, Skehel J, West S. Regulatory control of the resolution of DNA recombination intermediates during meiosis and mitosis. Cell. 2011;147:158-72 pubmed publisher
    ..In yeast meiosis, Mus81-Mms4 and Yen1 are controlled by phosphorylation events that lead to their sequential activation...
  12. Pardo B, Aguilera A. Complex chromosomal rearrangements mediated by break-induced replication involve structure-selective endonucleases. PLoS Genet. 2012;8:e1002979 pubmed publisher
    ..Using a newly designed substrate for the analysis of DSB-induced chromosomal translocations, we show that Mus81 and Yen1 structure-selective endonucleases (SSEs) promote BIR, thus causing NRTs...
  13. Ho C, Mazon G, Lam A, Symington L. Mus81 and Yen1 promote reciprocal exchange during mitotic recombination to maintain genome integrity in budding yeast. Mol Cell. 2010;40:988-1000 pubmed publisher
    ..products of mitotic recombination, we found a significant decrease in crossovers in the Saccharomyces cerevisiae mus81? mutant...
  14. Moriel Carretero M, Aguilera A. A postincision-deficient TFIIH causes replication fork breakage and uncovers alternative Rad51- or Pol32-mediated restart mechanisms. Mol Cell. 2010;37:690-701 pubmed publisher
    ..Our results define the genetic and molecular hallmarks of replication fork breakage and restart and bring insights to understand specific NER-related human syndromes. ..
  15. Bastin Shanower S, Fricke W, Mullen J, Brill S. The mechanism of Mus81-Mms4 cleavage site selection distinguishes it from the homologous endonuclease Rad1-Rad10. Mol Cell Biol. 2003;23:3487-96 pubmed
    b>Mus81-Mms4 and Rad1-Rad10 are homologous structure-specific endonucleases that cleave 3' branches from distinct substrates and are required for replication fork stability and nucleotide excision repair, respectively, in the yeast ..
  16. Gaskell L, Osman F, Gilbert R, Whitby M. Mus81 cleavage of Holliday junctions: a failsafe for processing meiotic recombination intermediates?. EMBO J. 2007;26:1891-901 pubmed
    ..Controversially, the Mus81-Eme1 endonuclease has been proposed to be an example of a eukaryotic nuclear resolvase...
  17. Smith C, Lam A, Symington L. Aberrant double-strand break repair resulting in half crossovers in mutants defective for Rad51 or the DNA polymerase delta complex. Mol Cell Biol. 2009;29:1432-41 pubmed publisher
    ..formed in Pol delta complex mutants show evidence of limited homology-dependent DNA synthesis and are partially Mus81 dependent, suggesting that strand invasion occurs and the stalled intermediate is subsequently cleaved...
  18. Kaliraman V, Mullen J, Fricke W, Bastin Shanower S, Brill S. Functional overlap between Sgs1-Top3 and the Mms4-Mus81 endonuclease. Genes Dev. 2001;15:2730-40 pubmed
    ..We have shown previously that yeast cells lacking SGS1 or TOP3 require MMS4 and MUS81 for viability...
  19. Schwartz E, Wright W, Ehmsen K, Evans J, Stahlberg H, Heyer W. Mus81-Mms4 functions as a single heterodimer to cleave nicked intermediates in recombinational DNA repair. Mol Cell Biol. 2012;32:3065-80 pubmed publisher
    The formation of crossovers is a fundamental genetic process. The XPF-family endonuclease Mus81-Mms4 (Eme1) contributes significantly to crossing over in eukaryotes...
  20. Ehmsen K, Heyer W. A junction branch point adjacent to a DNA backbone nick directs substrate cleavage by Saccharomyces cerevisiae Mus81-Mms4. Nucleic Acids Res. 2009;37:2026-36 pubmed publisher
    The DNA structure-selective endonuclease Mus81-Mms4/Eme1 incises a number of nicked joint molecule substrates in vitro. 3'-flaps are an excellent in vitro substrate for Mus81-Mms4/Eme1...
  21. Matulova P, Marini V, Burgess R, Sisakova A, Kwon Y, Rothstein R, et al. Cooperativity of Mus81.Mms4 with Rad54 in the resolution of recombination and replication intermediates. J Biol Chem. 2009;284:7733-45 pubmed publisher
    The Saccharomyces cerevisiae Mus81.Mms4 protein complex, a DNA structure-specific endonuclease, helps preserve genomic integrity by resolving pathological DNA structures that arise from damaged or aborted replication forks and may also ..
  22. Blanco M, Matos J, West S. Dual control of Yen1 nuclease activity and cellular localization by Cdk and Cdc14 prevents genome instability. Mol Cell. 2014;54:94-106 pubmed publisher
  23. Muñoz Galvan S, Tous C, Blanco M, Schwartz E, Ehmsen K, West S, et al. Distinct roles of Mus81, Yen1, Slx1-Slx4, and Rad1 nucleases in the repair of replication-born double-strand breaks by sister chromatid exchange. Mol Cell Biol. 2012;32:1592-603 pubmed publisher
    ..We analyzed the functional relevance of four structure-selective endonucleases (SSEs), Yen1, Mus81-Mms4, Slx1-Slx4, and Rad1, on SCR in Saccharomyces cerevisiae...
  24. Princz L, Wild P, Bittmann J, Aguado F, Blanco M, Matos J, et al. Dbf4-dependent kinase and the Rtt107 scaffold promote Mus81-Mms4 resolvase activation during mitosis. EMBO J. 2017;36:664-678 pubmed publisher
    ..In case of budding yeast Mus81-Mms4, this cell cycle stage-specific activation is known to depend on phosphorylation by CDK and Cdc5 kinases...
  25. Chang M, Bellaoui M, Zhang C, Desai R, Morozov P, Delgado Cruzata L, et al. RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex. EMBO J. 2005;24:2024-33 pubmed
    ..In addition, rmi1Delta strains fail to fully activate Rad53 upon exposure to DNA-damaging agents, suggesting that Rmi1 is also an important part of the Rad53-dependent DNA damage response. ..
  26. Sebesta M, Urulangodi M, Stefanovie B, Szakal B, Pacesa M, Lisby M, et al. Esc2 promotes Mus81 complex-activity via its SUMO-like and DNA binding domains. Nucleic Acids Res. 2017;45:215-230 pubmed publisher
    ..Here we show interaction between Esc2 and a structure-specific endonuclease Mus81-Mms4 (the Mus81 complex), their involvement in the metabolism of SCJs, and the effects Esc2 has on the enzymatic ..
  27. Panico E, Ede C, Schildmann M, Schürer K, Kramer W. Genetic evidence for a role of Saccharomyces cerevisiae Mph1 in recombinational DNA repair under replicative stress. Yeast. 2010;27:11-27 pubmed publisher
    ..Finally, we observe an epistatic relationship in terms of sensitivity to camptothecin of mms4 or mus81 to mph1. Overall, our results support a role of Mph1 in assisting replication progression...
  28. Saugar I, Jiménez Martín A, Tercero J. Subnuclear Relocalization of Structure-Specific Endonucleases in Response to DNA Damage. Cell Rep. 2017;20:1553-1562 pubmed publisher
    ..Here, we show that the structure-specific endonuclease Mus81-Mms4/EME1 relocalizes to subnuclear foci following DNA damage and colocalizes with the endonucleases Rad1-Rad10 (..
  29. Deng C, Brown J, You D, Brown J. Multiple endonucleases function to repair covalent topoisomerase I complexes in Saccharomyces cerevisiae. Genetics. 2005;170:591-600 pubmed
    ..Previous work has demonstrated that two structure-specific nucleases, Rad1 and Mus81, protect cells from camptothecin toxicity...
  30. Mischo H, Gómez González B, Grzechnik P, Rondon A, Wei W, Steinmetz L, et al. Yeast Sen1 helicase protects the genome from transcription-associated instability. Mol Cell. 2011;41:21-32 pubmed publisher
    ..Based on these findings, we propose that R loop formation is a frequent event during transcription and a key function of Sen1 is to prevent their accumulation and associated genome instability. ..
  31. Mayle R, Campbell I, Beck C, Yu Y, Wilson M, Shaw C, et al. DNA REPAIR. Mus81 and converging forks limit the mutagenicity of replication fork breakage. Science. 2015;349:742-7 pubmed publisher
    ..Pol32-dependent synthesis, but that mutagenic synthesis is limited to within a few kilobases from the break by Mus81 endonuclease and a converging fork...
  32. Ang J, Duffy S, Segovia R, Stirling P, Hieter P. Dosage Mutator Genes in Saccharomyces cerevisiae: A Novel Mutator Mode-of-Action of the Mph1 DNA Helicase. Genetics. 2016;204:975-986 pubmed publisher
    ..We believe this represents a novel mutator mode-of-action and opens up new prospects to understand how upregulation of DNA repair proteins may contribute to mutagenesis. ..
  33. Menolfi D, Delamarre A, Lengronne A, Pasero P, Branzei D. Essential Roles of the Smc5/6 Complex in Replication through Natural Pausing Sites and Endogenous DNA Damage Tolerance. Mol Cell. 2015;60:835-46 pubmed publisher
  34. Haber J, Braberg H, Wu Q, Alexander R, Haase J, Ryan C, et al. Systematic triple-mutant analysis uncovers functional connectivity between pathways involved in chromosome regulation. Cell Rep. 2013;3:2168-78 pubmed publisher
    ..This approach can reveal functional redundancies that cannot be uncovered through traditional double-mutant analyses. ..
  35. Böhm S, Szakal B, Herken B, Sullivan M, Mihalevic M, Kabbinavar F, et al. The Budding Yeast Ubiquitin Protease Ubp7 Is a Novel Component Involved in S Phase Progression. J Biol Chem. 2016;291:4442-52 pubmed publisher
    ..In summary, our results suggest that Ubp7 contributes to S phase progression by affecting the chromatin state at replication forks, and we propose histone H2B ubiquitination as a potential substrate of Ubp7. ..
  36. Ii M, Ii T, Mironova L, Brill S. Epistasis analysis between homologous recombination genes in Saccharomyces cerevisiae identifies multiple repair pathways for Sgs1, Mus81-Mms4 and RNase H2. Mutat Res. 2011;714:33-43 pubmed publisher
    The DNA repair genes SGS1 and MUS81 of Saccharomyces cerevisiae are thought to control alternative pathways for the repair of toxic recombination intermediates based on the fact that sgs1? mus81? synthetic lethality is suppressed in the ..
  37. Keyamura K, Arai K, Hishida T. Srs2 and Mus81-Mms4 Prevent Accumulation of Toxic Inter-Homolog Recombination Intermediates. PLoS Genet. 2016;12:e1006136 pubmed publisher
    ..The structure-specific complex of crossover junction endonucleases Mus81 and Mms4 was also required for viability of diploid, but not haploid, SRS2 deletion mutants (srs2?), and diploid ..
  38. Kramarz K, Litwin I, Cal Bakowska M, Szakal B, Branzei D, Wysocki R, et al. Swi2/Snf2-like protein Uls1 functions in the Sgs1-dependent pathway of maintenance of rDNA stability and alleviation of replication stress. DNA Repair (Amst). 2014;21:24-35 pubmed publisher
    ..implicated in DNA repair independently of the replication stress response pathways mediated by the endonucleases Mus81 and Yen1 and the helicases Mph1 and Srs2...
  39. Colosio A, Frattini C, Pellicanò G, Villa Hernández S, Bermejo R. Nucleolytic processing of aberrant replication intermediates by an Exo1-Dna2-Sae2 axis counteracts fork collapse-driven chromosome instability. Nucleic Acids Res. 2016;44:10676-10690 pubmed
    ..Genetic analyses demonstrated a functional interplay of Exo1 with Mus81, Dna2 and Sae2 nucleases in promoting cell survival following replication stress, suggestive of concerted ..
  40. Kim J, Harris S, Dinter T, Shah K, Mirkin S. The role of break-induced replication in large-scale expansions of (CAG)n/(CTG)n repeats. Nat Struct Mol Biol. 2017;24:55-60 pubmed publisher
    ..Genetic analysis revealed that Rad51, Rad52, Mre11, Pol32, Pif1, and Mus81 and/or Yen1 proteins are required for large-scale expansions, whereas proteins previously implicated in small-..
  41. Kramarz K, Mucha S, Litwin I, Barg Wojas A, Wysocki R, Dziadkowiec D. DNA Damage Tolerance Pathway Choice Through Uls1 Modulation of Srs2 SUMOylation in Saccharomyces cerevisiae. Genetics. 2017;206:513-525 pubmed publisher
    ..Conversely, SUMOylation of Srs2 attenuates its interaction with PCNA Sgs1 helicase and Mus81 endonuclease are crucial for disentanglement of repair intermediates at the replication fork...
  42. Chavdarova M, Marini V, Sisakova A, Sedlackova H, Vigasová D, Brill S, et al. Srs2 promotes Mus81-Mms4-mediated resolution of recombination intermediates. Nucleic Acids Res. 2015;43:3626-42 pubmed publisher
    ..Recovery of such forks is essential for the maintenance of genomic stability. The structure-specific endonuclease Mus81-Mms4 has been implicated in processing DNA intermediates that arise from collapsed forks and homologous ..
  43. Putnam C, Hayes T, Kolodner R. Post-replication repair suppresses duplication-mediated genome instability. PLoS Genet. 2010;6:e1000933 pubmed publisher
    ..Our analysis is consistent with models in which PRR prevents replication damage from becoming double strand breaks (DSBs) and/or regulates the activity of HR on DSBs. ..
  44. Stafa A, Donnianni R, Timashev L, Lam A, Symington L. Template switching during break-induced replication is promoted by the Mph1 helicase in Saccharomyces cerevisiae. Genetics. 2014;196:1017-28 pubmed publisher
    ..In addition, we show that the Mus81, Rad1, and Yen1 structure-selective nucleases act redundantly to facilitate BIR.
  45. Cal Bakowska M, Litwin I, Bocer T, Wysocki R, Dziadkowiec D. The Swi2-Snf2-like protein Uls1 is involved in replication stress response. Nucleic Acids Res. 2011;39:8765-77 pubmed publisher
    ..Consistently, cells lacking Uls1 and endonuclease Mus81 were more sensitive to HU, MMS and CPT than single mus81?...
  46. Ölmezer G, Klein D, Rass U. DNA repair defects ascribed to pby1 are caused by disruption of Holliday junction resolvase Mus81-Mms4. DNA Repair (Amst). 2015;33:17-23 pubmed publisher
    ..On the basis of genetic interaction profile similarity, we pinpoint disruption of Holliday junction resolvase Mus81-Mms4 as the mutation responsible for DNA repair phenotypes currently ascribed to pby1...
  47. Guillet M, Boiteux S. Endogenous DNA abasic sites cause cell death in the absence of Apn1, Apn2 and Rad1/Rad10 in Saccharomyces cerevisiae. EMBO J. 2002;21:2833-41 pubmed
    ..The activation of this checkpoint is RAD9 dependent and allows residual DNA repair. The Mus81/Mms4 complex was identified as one of these back-up repair activities...
  48. Thu H, Nguyen T, Munashingha P, Kwon B, Dao Van Q, Seo Y. A physiological significance of the functional interaction between Mus81 and Rad27 in homologous recombination repair. Nucleic Acids Res. 2015;43:1684-99 pubmed publisher
    Fen1 and Mus81-Mms4 are endonucleases involved in the processing of various DNA structural intermediates, and they were shown to have genetic and functional interactions with each other...
  49. Eissler C, Mazon G, Powers B, Savinov S, Symington L, Hall M. The Cdk/cDc14 module controls activation of the Yen1 holliday junction resolvase to promote genome stability. Mol Cell. 2014;54:80-93 pubmed publisher
    ..The precise timing of Yen1 activation, governed by core cell-cycle regulators, helps coordinate DNA repair with chromosome segregation and safeguards against genome destabilization. ..
  50. Allen Soltero S, Martinez S, Putnam C, Kolodner R. A saccharomyces cerevisiae RNase H2 interaction network functions to suppress genome instability. Mol Cell Biol. 2014;34:1521-34 pubmed publisher
    ..This analysis suggests that cells with RNase H2 defects have increased levels of DNA damage and depend on other pathways of DNA metabolism to overcome the deleterious effects of this DNA damage. ..
  51. Herrero A, Moreno S. Lsm1 promotes genomic stability by controlling histone mRNA decay. EMBO J. 2011;30:2008-18 pubmed publisher
    ..Our results demonstrate that improper histone stoichiometry leads to genomic instability and highlight the importance of regulating histone mRNA decay in the tight control of histone levels in yeast. ..
  52. Saugar I, V zquez M, Gallo Fern ndez M, Ortiz Baz n M, Segurado M, Calzada A, et al. Temporal regulation of the Mus81-Mms4 endonuclease ensures cell survival under conditions of DNA damage. Nucleic Acids Res. 2013;41:8943-58 pubmed publisher
    The structure-specific Mus81-Eme1/Mms4 endonuclease contributes importantly to DNA repair and genome integrity maintenance...
  53. Yin Y, Petes T. Recombination between homologous chromosomes induced by unrepaired UV-generated DNA damage requires Mus81p and is suppressed by Mms2p. PLoS Genet. 2015;11:e1005026 pubmed publisher
    ..We found that the resolvase Mus81p is required for most of the UV-induced inter-homolog recombination events...
  54. García Luis J, Machín F. Mus81-Mms4 and Yen1 resolve a novel anaphase bridge formed by noncanonical Holliday junctions. Nat Commun. 2014;5:5652 pubmed publisher
    ..Here we report that the overlapping actions of the structure-selective endonucleases (SSEs) Mus81-Mms4/EME1 and Yen1/GEN1, but not Slx1-Slx4, are also essential to prevent the formation of spontaneous SCBs that ..
  55. Oke A, Anderson C, Yam P, Fung J. Controlling meiotic recombinational repair - specifying the roles of ZMMs, Sgs1 and Mus81/Mms4 in crossover formation. PLoS Genet. 2014;10:e1004690 pubmed publisher
    ..Our analysis suggests that Mus81/Mms4 (Eme1), rather than just being a minor resolvase for COs is crucial for both COs and NCOs in preventing ..
  56. Mukherjee S, Wright W, Ehmsen K, Heyer W. The Mus81-Mms4 structure-selective endonuclease requires nicked DNA junctions to undergo conformational changes and bend its DNA substrates for cleavage. Nucleic Acids Res. 2014;42:6511-22 pubmed publisher
    b>Mus81-Mms4/EME1 is a DNA structure-selective endonuclease that cleaves joint DNA molecules that form during homologous recombination in mitotic and meiotic cells...
  57. Chin J, Bashkirov V, Heyer W, Romesberg F. Esc4/Rtt107 and the control of recombination during replication. DNA Repair (Amst). 2006;5:618-28 pubmed
    ..Thus, we propose that Esc4 associates with ssDNA of stalled forks and acts as a scaffolding protein to recruit and/or modulate the function of other proteins required to reinitiate DNA synthesis. ..
  58. Díaz de la Loza M, Gallardo M, García Rubio M, Izquierdo A, Herrero E, Aguilera A, et al. Zim17/Tim15 links mitochondrial iron-sulfur cluster biosynthesis to nuclear genome stability. Nucleic Acids Res. 2011;39:6002-15 pubmed publisher
    ..We propose that compromised ribosome biosynthesis and cell-cycle progression are interconnected, together contributing to replicative stress and nuclear genome instability in zim17? mutants. ..