Gene Symbol: MTF2
Alias: NAM1, Mtf2p
Species: Saccharomyces cerevisiae S288c
Wallis M, Groudinsky O, Slonimski P, Dujardin G. The NAM1 protein (NAM1p), which is selectively required for cox1, cytb and atp6 transcript processing/stabilisation, is located in the yeast mitochondrial matrix. Eur J Biochem. 1994;222:27-32 pubmed
The NAM1 nuclear gene was shown to control the stability and/or processing of mitochondrial transcripts of the cytochrome b, cytochrome oxidase subunit I and ATP synthase subunit VI genes [Groudinsky O., Bousquet I., Wallis M. G...
Rodeheffer M, Boone B, Bryan A, Shadel G. Nam1p, a protein involved in RNA processing and translation, is coupled to transcription through an interaction with yeast mitochondrial RNA polymerase. J Biol Chem. 2001;276:8616-22 pubmed
..steady-state levels of mature mitochondrial CYTB and COXI transcripts, which is a primary defect observed in NAM1 null mutant yeast strains...
Bryan A, Rodeheffer M, Wearn C, Shadel G. Sls1p is a membrane-bound regulator of transcription-coupled processes involved in Saccharomyces cerevisiae mitochondrial gene expression. Genetics. 2002;160:75-82 pubmed
..This was supported by the observation that SLS1 overexpression rescued the petite phenotype of a NAM1 null mutation...
Rodeheffer M, Shadel G. Multiple interactions involving the amino-terminal domain of yeast mtRNA polymerase determine the efficiency of mitochondrial protein synthesis. J Biol Chem. 2003;278:18695-701 pubmed
Markov D, Savkina M, Anikin M, Del Campo M, Ecker K, Lambowitz A, et al
. Identification of proteins associated with the yeast mitochondrial RNA polymerase by tandem affinity purification. Yeast. 2009;26:423-40 pubmed publisher
..Further, we found that Mss116p inhibits transcription by mtRNAP in vitro in a steady-state reaction. Our results support the hypothesis that Mss116p and Pet127p are involved in modulation of mtRNAP activity. ..
Asher E, Groudinsky O, Dujardin G, Altamura N, Kermorgant M, Slonimski P. Novel class of nuclear genes involved in both mRNA splicing and protein synthesis in Saccharomyces cerevisiae mitochondria. Mol Gen Genet. 1989;215:517-28 pubmed
We have cloned three distinct nuclear genes, NAM1, NAM7, and NAM8, which alleviate mitochondrial intron mutations of the cytochrome b and COXI (subunit I of cytochrome oxidase) genes when present on multicopy plasmids...
Groudinsky O, Bousquet I, Wallis M, Slonimski P, Dujardin G. The NAM1/MTF2 nuclear gene product is selectively required for the stability and/or processing of mitochondrial transcripts of the atp6 and of the mosaic, cox1 and cytb genes in Saccharomyces cerevisiae. Mol Gen Genet. 1993;240:419-27 pubmed
The NAM1/MTF2 gene was firstly isolated as a multicopy suppressor of mitochondrial splicing deficiencies and independently as a gene of which a thermosensitive allele affects mitochondrial transcription in organello...
Li G, Becam A, Slonimski P, Herbert C. In vitro mutagenesis of the mitochondrial leucyl tRNA synthetase of Saccharomyces cerevisiae shows that the suppressor activity of the mutant proteins is related to the splicing function of the wild-type protein. Mol Gen Genet. 1996;252:667-75 pubmed
..These data show that the splicing and suppressor functions are linked and that the suppressor activity of the mutant alleles results from a modification of the wild-type splicing activity. ..
Naithani S, Saracco S, Butler C, Fox T. Interactions among COX1, COX2, and COX3 mRNA-specific translational activator proteins on the inner surface of the mitochondrial inner membrane of Saccharomyces cerevisiae. Mol Biol Cell. 2003;14:324-33 pubmed
..In addition, we found interactions between Nam1p/Mtf2p and the translational activators, suggesting an organized delivery of mitochondrial mRNAs to the translation ..