Gene Symbol: MSH5
Description: MutS family protein MSH5
Alias: MutS family protein MSH5
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Agarwal S, Roeder G. Zip3 provides a link between recombination enzymes and synaptonemal complex proteins. Cell. 2000;102:245-55 pubmed meiotic recombination, including proteins that act at both early (Mre11, Rad51, and Rad57) and late (Msh4 and Msh5) steps in the exchange process...
  2. Oh S, Lao J, Hwang P, Taylor A, Smith G, Hunter N. BLM ortholog, Sgs1, prevents aberrant crossing-over by suppressing formation of multichromatid joint molecules. Cell. 2007;130:259-72 pubmed
    ..Sgs1 and procrossover factors, Msh5 and Mlh3, are antagonistic since Sgs1 prevents dHJ formation in msh5 cells and sgs1 mutation alleviates crossover ..
  3. Zanders S, Alani E. The pch2Delta mutation in baker's yeast alters meiotic crossover levels and confers a defect in crossover interference. PLoS Genet. 2009;5:e1000571 pubmed publisher
    ..Third, crossovers observed in pch2Delta require both Msh4-Msh5 and Mms4-Mus81 functions...
  4. Argueso J, Wanat J, Gemici Z, Alani E. Competing crossover pathways act during meiosis in Saccharomyces cerevisiae. Genetics. 2004;168:1805-16 pubmed
    In Saccharomyces cerevisiae the MSH4-MSH5, MLH1-MLH3, and MUS81-MMS4 complexes act to promote crossing over during meiosis. MSH4-MSH5, but not MUS81-MMS4, promotes crossovers that display interference...
  5. Wanat J, Kim K, Koszul R, Zanders S, Weiner B, Kleckner N, et al. Csm4, in collaboration with Ndj1, mediates telomere-led chromosome dynamics and recombination during yeast meiosis. PLoS Genet. 2008;4:e1000188 pubmed publisher
  6. Pochart P, Woltering D, Hollingsworth N. Conserved properties between functionally distinct MutS homologs in yeast. J Biol Chem. 1997;272:30345-9 pubmed
    ..MSH2, 3, and 6 function in mismatch repair in both vegetative and meiotic cells, whereas MSH4 and MSH5 act specifically to facilitate crossovers between homologs during meiosis...
  7. Börner G, Kleckner N, Hunter N. Crossover/noncrossover differentiation, synaptonemal complex formation, and regulatory surveillance at the leptotene/zygotene transition of meiosis. Cell. 2004;117:29-45 pubmed
    Yeast mutants lacking meiotic proteins Zip1, Zip2, Zip3, Mer3, and/or Msh5 (ZMMs) were analyzed for recombination, synaptonemal complex (SC), and meiotic progression...
  8. Lao J, Oh S, Shinohara M, Shinohara A, Hunter N. Rad52 promotes postinvasion steps of meiotic double-strand-break repair. Mol Cell. 2008;29:517-24 pubmed publisher
    ..Our findings show that Rad52 functions in temporally and biochemically distinct reactions and suggest a general annealing mechanism for reuniting DSB ends during recombination. ..
  9. Rakshambikai R, Srinivasan N, Nishant K. Structural insights into Saccharomyces cerevisiae Msh4-Msh5 complex function using homology modeling. PLoS ONE. 2013;8:e78753 pubmed publisher
    The Msh4-Msh5 protein complex in eukaryotes is involved in stabilizing Holliday junctions and its progenitors to facilitate crossing over during Meiosis I...

More Information


  1. Thacker D, Mohibullah N, Zhu X, Keeney S. Homologue engagement controls meiotic DNA break number and distribution. Nature. 2014;510:241-6 pubmed publisher
    ..Thus, feedback tied to ZMM function contributes in unexpected ways to spatial patterning of recombination. ..
  2. Chan A, Borts R, Hoffmann E. Temperature-dependent modulation of chromosome segregation in msh4 mutants of budding yeast. PLoS ONE. 2009;4:e7284 pubmed publisher
    ..In budding yeast, the evolutionarily conserved mismatch-repair paralogues, Msh4 and Msh5, promote crossover formation in conjunction with several other proteins, collectively termed the Synapsis ..
  3. Nishant K, Chen C, Shinohara M, Shinohara A, Alani E. Genetic analysis of baker's yeast Msh4-Msh5 reveals a threshold crossover level for meiotic viability. PLoS Genet. 2010;6: pubmed publisher
    During meiosis, the Msh4-Msh5 complex is thought to stabilize single-end invasion intermediates that form during early stages of recombination and subsequently bind to Holliday junctions to facilitate crossover formation...
  4. Sonntag Brown M, Lim E, Chen C, Nishant K, Alani E. Genetic analysis of mlh3 mutations reveals interactions between crossover promoting factors during meiosis in baker's yeast. G3 (Bethesda). 2013;3:9-22 pubmed publisher
    ..In baker's yeast, two heterodimeric complexes, Msh4-Msh5 and Mlh1-Mlh3, act in meiosis to promote interference-dependent crossing over...
  5. Hong E, Roeder G. A role for Ddc1 in signaling meiotic double-strand breaks at the pachytene checkpoint. Genes Dev. 2002;16:363-76 pubmed
    ..The Rad24 checkpoint protein interacts with Ddc1 and with recombination proteins (Sae1, Sae2, Rad57, and Msh5) in the two-hybrid protein system, suggesting that Rad24 also functions at DSB sites...
  6. Voelkel Meiman K, Cheng S, Morehouse S, MacQueen A. Synaptonemal Complex Proteins of Budding Yeast Define Reciprocal Roles in MutS?-Mediated Crossover Formation. Genetics. 2016;203:1091-103 pubmed publisher
    ..The latter class directly or indirectly limits MutS?-mediated crossovers along meiotic chromosomes. Our findings illustrate how reciprocal roles in crossover recombination can be simultaneously linked to the SC structure. ..