Gene Symbol: MSG5
Description: tyrosine/serine/threonine protein phosphatase MSG5
Alias: tyrosine/serine/threonine protein phosphatase MSG5
Species: Saccharomyces cerevisiae S288c
Products:     MSG5

Top Publications

  1. Doi K, Gartner A, Ammerer G, Errede B, Shinkawa H, Sugimoto K, et al. MSG5, a novel protein phosphatase promotes adaptation to pheromone response in S. cerevisiae. EMBO J. 1994;13:61-70 pubmed
    ..Overexpression of a novel gene called MSG5 suppresses this inhibition of cell division...
  2. Marin M, Flandez M, Bermejo C, Arroyo J, Martín H, Molina M. Different modulation of the outputs of yeast MAPK-mediated pathways by distinct stimuli and isoforms of the dual-specificity phosphatase Msg5. Mol Genet Genomics. 2009;281:345-59 pubmed publisher
    ..The yeast dual-specificity phosphatase (DSP) Msg5, expressed as two isoforms of different length, dephosphorylates the MAPKs of mating and cell integrity pathways, ..
  3. Remenyi A, Good M, Bhattacharyya R, Lim W. The role of docking interactions in mediating signaling input, output, and discrimination in the yeast MAPK network. Mol Cell. 2005;20:951-62 pubmed
    ..Induced fit recognition may allow docking peptides to achieve discrimination by exploiting subtle differences in kinase flexibility. ..
  4. Flandez M, Cosano I, Nombela C, Martín H, Molina M. Reciprocal regulation between Slt2 MAPK and isoforms of Msg5 dual-specificity protein phosphatase modulates the yeast cell integrity pathway. J Biol Chem. 2004;279:11027-34 pubmed
    ..Here we show that Msg5 DSP activity is essential for maintaining a low level of signaling through the cell integrity pathway in ..
  5. Blackwell E, Halatek I, Kim H, Ellicott A, Obukhov A, Stone D. Effect of the pheromone-responsive G(alpha) and phosphatase proteins of Saccharomyces cerevisiae on the subcellular localization of the Fus3 mitogen-activated protein kinase. Mol Cell Biol. 2003;23:1135-50 pubmed
    ..was also inhibited by overexpression of either wild-type Gpa1, the E364K hyperadaptive mutant form of Gpa1, or the Msg5 dually specific phosphatase. The effects of Gpa1 and Msg5 on Fus3 are partially interdependent...
  6. Martin H, Rodríguez Pachón J, Ruiz C, Nombela C, Molina M. Regulatory mechanisms for modulation of signaling through the cell integrity Slt2-mediated pathway in Saccharomyces cerevisiae. J Biol Chem. 2000;275:1511-9 pubmed of the phosphatases involved in Slt2 dephosphorylation is likely to be the dual specificity phosphatase Msg5, since overexpression of MSG5 in a sac7Delta mutant eliminates the high Slt2 phosphorylation, and disruption of ..
  7. Hahn J, Thiele D. Regulation of the Saccharomyces cerevisiae Slt2 kinase pathway by the stress-inducible Sdp1 dual specificity phosphatase. J Biol Chem. 2002;277:21278-84 pubmed
    ..via dephosphorylation by two protein-tyrosine phosphatases, Ptp2 and Ptp3, and a dual specificity phosphatase, Msg5. In this study, we provide genetic and biochemical evidence that the stress-inducible dual specificity phosphatase, ..
  8. Palacios L, Dickinson R, Sacristán Reviriego A, Didmon M, Marin M, Martín H, et al. Distinct docking mechanisms mediate interactions between the Msg5 phosphatase and mating or cell integrity mitogen-activated protein kinases (MAPKs) in Saccharomyces cerevisiae. J Biol Chem. 2011;286:42037-50 pubmed publisher
    ..For example, the yeast dual specificity phosphatase Msg5 dephosphorylates the Fus3 and Slt2 MAPKs operating in the mating and cell wall integrity pathways, respectively...
  9. Alonso Rodríguez E, Fernández Piñar P, Sacristán Reviriego A, Molina M, Martín H. An Analog-sensitive Version of the Protein Kinase Slt2 Allows Identification of Novel Targets of the Yeast Cell Wall Integrity Pathway. J Biol Chem. 2016;291:5461-72 pubmed publisher
    ..Taking advantage of this chemical-genetic approach, we found that Slt2 phosphorylates the MAPK phosphatase Msg5 both in the N-terminal regulatory and C-terminal catalytic domains...

More Information


  1. Davenport K, Williams K, Ullmann B, Gustin M. Activation of the Saccharomyces cerevisiae filamentation/invasion pathway by osmotic stress in high-osmolarity glycogen pathway mutants. Genetics. 1999;153:1091-103 pubmed
    ..Three high-copy suppressors of pbs2-3 osmosensitivity were identified: MSG5, CAK1, and TRX1...
  2. Leza M, Elion E. POG1, a novel yeast gene, promotes recovery from pheromone arrest via the G1 cyclin CLN2. Genetics. 1999;151:531-43 pubmed
    ..We have isolated a novel gene, POG1, whose promotion of recovery parallels that of the MAPK phosphatase Msg5. POG1 confers alpha-factor resistance when overexpressed and enhances alpha-factor sensitivity when deleted in the ..
  3. Hermansyah -, Laviña W, Sugiyama M, Kaneko Y, Harashima S. Identification of protein kinase disruptions as suppressors of the calcium sensitivity of S. cerevisiae Deltaptp2 Deltamsg5 protein phosphatase double disruptant. Arch Microbiol. 2010;192:157-65 pubmed publisher
    ..cerevisiae protein phosphatase (PPase) genes, PTP2 (phosphotyrosine-specific PPase) and MSG5 (phosphotyrosine and phosphothreonine/serine-PPase) causes calcium-sensitive growth (Cas)...
  4. Hermansyah -, Sugiyama M, Kaneko Y, Harashima S. Yeast protein phosphatases Ptp2p and Msg5p are involved in G1-S transition, CLN2 transcription, and vacuole morphogenesis. Arch Microbiol. 2009;191:721-33 pubmed publisher
    ..reported that double disruption of protein phosphatase (PPase) genes PTP2 (phosphotyrosine-specific PPase) and MSG5 (phosphotyrosine and phosphothreonine/serine-PPase) causes Ca(2+) sensitive growth, whereas the single disruptions ..
  5. Blackwell E, Kim H, Stone D. The pheromone-induced nuclear accumulation of the Fus3 MAPK in yeast depends on its phosphorylation state and on Dig1 and Dig2. BMC Cell Biol. 2007;8:44 pubmed
    ..We have previously reported that overexpression of the Msg5 phosphatase inhibits the nuclear localization of Fus3...
  6. Nagiec M, McCarter P, Kelley J, Dixit G, Elston T, Dohlman H. Signal inhibition by a dynamically regulated pool of monophosphorylated MAPK. Mol Biol Cell. 2015;26:3359-71 pubmed publisher
    ..Conversely, cells lacking the dual-specificity phosphatase (msg5Δ) or that are deficient in docking to the MAPK-scaffold (Ste5(ND)) accumulate a greater proportion of dual-..
  7. Laviña W, Hermansyah -, Sugiyama M, Kaneko Y, Harashima S. Functionally redundant protein phosphatase genes PTP2 and MSG5 co-regulate the calcium signaling pathway in Saccharomyces cerevisiae upon exposure to high extracellular calcium concentration. J Biosci Bioeng. 2013;115:138-46 pubmed publisher
    ..We have previously reported that the disruption of two protein phosphatase (PPase) genes, PTP2 and MSG5, causes calcium sensitivity indicating that functional redundancy exists between the two PPases in response to high ..
  8. Yamamoto K, Tatebayashi K, Tanaka K, Saito H. Dynamic control of yeast MAP kinase network by induced association and dissociation between the Ste50 scaffold and the Opy2 membrane anchor. Mol Cell. 2010;40:87-98 pubmed publisher
    ..Thus, dynamic regulation of Ste50-Opy2 interaction fine-tunes the MAPK signaling network. ..
  9. Watanabe Y, Irie K, Matsumoto K. Yeast RLM1 encodes a serum response factor-like protein that may function downstream of the Mpk1 (Slt2) mitogen-activated protein kinase pathway. Mol Cell Biol. 1995;15:5740-9 pubmed
    ..A screening for dosage suppressors yielded the MSG5 genes, which encode a dual-specificity protein phosphatase...
  10. Protchenko O, Rodriguez Suarez R, Androphy R, Bussey H, Philpott C. A screen for genes of heme uptake identifies the FLC family required for import of FAD into the endoplasmic reticulum. J Biol Chem. 2006;281:21445-57 pubmed
    ..We propose that the FLC genes are required for import of FAD into the lumen of the endoplasmic reticulum, where it is required for disulfide bond formation. ..
  11. Parrish W, Stefan C, Emr S. PtdIns(3)P accumulation in triple lipid-phosphatase-deletion mutants triggers lethal hyperactivation of the Rho1p/Pkc1p cell-integrity MAP kinase pathway. J Cell Sci. 2005;118:5589-601 pubmed
    ..Taken together, the results of this study indicated that aberrant Rho1p/Pkc1p signaling contributes to the lethal effects of PtdIns(3)P accumulation in cells deficient in PI 3-phosphatase activity. ..
  12. Sakumoto N, Matsuoka I, Mukai Y, Ogawa N, Kaneko Y, Harashima S. A series of double disruptants for protein phosphatase genes in Saccharomyces cerevisiae and their phenotypic analysis. Yeast. 2002;19:587-99 pubmed
    ..The msg5 ptp2 double disruptant, like the ynr022c single one, did not grow on complete medium containing 0.3 M CaCl(2)...
  13. Maeder C, Hink M, Kinkhabwala A, Mayr R, Bastiaens P, Knop M. Spatial regulation of Fus3 MAP kinase activity through a reaction-diffusion mechanism in yeast pheromone signalling. Nat Cell Biol. 2007;9:1319-26 pubmed
    ..Propagation of signalling from the shmoo is, therefore, spatially constrained by a gradient-generating reaction-diffusion mechanism. ..
  14. Vink E, Vossen J, Ram A, van den Ende H, Brekelmans S, de Nobel H, et al. The protein kinase Kic1 affects 1,6-beta-glucan levels in the cell wall of Saccharomyces cerevisiae. Microbiology. 2002;148:4035-48 pubmed
    ..It is proposed that Kic1 affects cell wall construction in multiple ways and in particular in regulating 1,6-beta-glucan levels in the wall. ..