Gene Symbol: MSB4
Description: Rab GTPase-activating protein MSB4
Alias: Rab GTPase-activating protein MSB4
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Bi E, Chiavetta J, Chen H, Chen G, Chan C, Pringle J. Identification of novel, evolutionarily conserved Cdc42p-interacting proteins and of redundant pathways linking Cdc24p and Cdc42p to actin polarization in yeast. Mol Biol Cell. 2000;11:773-93 pubmed
    ..MSB3 has a close homologue (designated MSB4) and two more distant homologues (MDR1 and YPL249C) in S...
  2. Albert S, Gallwitz D. Msb4p, a protein involved in Cdc42p-dependent organization of the actin cytoskeleton, is a Ypt/Rab-specific GAP. Biol Chem. 2000;381:453-6 pubmed
    ..Purified Msb4p/Gyp4p acts primarily on Sec4p, Ypt6p and Ypt7p and might have a role in polarized secretion. ..
  3. Tcheperegine S, Gao X, Bi E. Regulation of cell polarity by interactions of Msb3 and Msb4 with Cdc42 and polarisome components. Mol Cell Biol. 2005;25:8567-80 pubmed
    ..Here we show that the Rab GTPase-activating proteins (GAPs) Msb3 and Msb4 interact directly with Spa2, a scaffold protein of the "polarisome" that also interacts with the formin ..
  4. Iwanejko L, Smith K, Loeillet S, Nicolas A, Fabre F. Disruption and functional analysis of six ORFs on chromosome XV: YOL117w, YOL115w ( TRF4), YOL114c, YOL112w ( MSB4), YOL111c and YOL072w. Yeast. 1999;15:1529-39 pubmed
    ..Disruption of three of the ORFs-YOL117w, YOL114c, and YOL112w (also known as MSB4)-does not result in any noteworthy phenotype with respect to temperature or nutritional requirements, but yol112w ..
  5. Bach S, Bouchat O, Portetelle D, Vandenbol M. Co-deletion of the MSB3 and MSB4 coding regions affects bipolar budding and perturbs the organization of the actin cytoskeleton. Yeast. 2000;16:1015-23 pubmed
    ..functions of these proteins, we constructed single and double yeast mutants by disrupting the MSB3 gene, the MSB4 gene, or both...
  6. Gao X, Albert S, Tcheperegine S, Burd C, Gallwitz D, Bi E. The GAP activity of Msb3p and Msb4p for the Rab GTPase Sec4p is required for efficient exocytosis and actin organization. J Cell Biol. 2003;162:635-46 pubmed
    ..Using a strain defective in polarized secretion and actin-patch organization, we showed that a change in actin-patch organization could be a consequence of the fusion of mistargeted vesicles with the plasma membrane. ..
  7. Bizimungu C, De Neve N, Burny A, Bach S, Bontemps F, Portetelle D, et al. Expression in a RabGAP yeast mutant of two human homologues, one of which is an oncogene. Biochem Biophys Res Commun. 2003;310:498-504 pubmed
    ..the biochemical function of Tre2 oncogene, we expressed its cDNA and, as a control, the RN-tre cDNA, in an msb3 msb4 double mutant yeast strain...
  8. Bizimungu C, Vandenbol M. At least two regions of the oncoprotein Tre2 are involved in its lack of GAP activity. Biochem Biophys Res Commun. 2005;335:883-90 pubmed
    ..These complementation experiments revealed, in addition to the TBC domain, a second Tre2 region involved in the oncoprotein's lack of GAP activity: a 93-aa region flanking the TBC domain on the C-terminal side. ..
  9. Dechamps C, Portetelle D, Vandenbol M. Multicopy suppression screen in the msb3 msb4 Saccharomyces cerevisiae double mutant, affected in Ypt/RabGAP activity. Biotechnol Lett. 2005;27:1439-49 pubmed
    ..The msb3 msb4 yeast double mutation causes growth inhibition in the presence of DMSO and/or caffeine, affects the organization of ..

More Information


  1. Kozminski K, Alfaro G, Dighe S, Beh C. Homologues of oxysterol-binding proteins affect Cdc42p- and Rho1p-mediated cell polarization in Saccharomyces cerevisiae. Traffic. 2006;7:1224-42 pubmed
    ..Disruption of all OSH gene function caused specific defects in polarized exocytosis, indicating that the Osh proteins are collectively required for a secretory pathway implicated in the maintenance of polarized growth. ..
  2. Zanolari B, Rockenbauch U, Trautwein M, Clay L, Barral Y, Spang A. Transport to the plasma membrane is regulated differently early and late in the cell cycle in Saccharomyces cerevisiae. J Cell Sci. 2011;124:1055-66 pubmed publisher
    ..We propose that transport to and from the bud neck is regulated differently in small- and large-budded cells, and differs early and late in the cell cycle. ..
  3. Li X, Ferro Novick S, Novick P. Different polarisome components play distinct roles in Slt2p-regulated cortical ER inheritance in Saccharomyces cerevisiae. Mol Biol Cell. 2013;24:3145-54 pubmed publisher
    ..Our results point to a common mechanism for the regulation of ER inheritance in which Slt2p activity at the bud tip controls the association of the ER with the actin-based cytoskeleton...