Gene Symbol: MSB3
Description: Rab GTPase-activating protein MSB3
Alias: GYP3, Rab GTPase-activating protein MSB3
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Tcheperegine S, Gao X, Bi E. Regulation of cell polarity by interactions of Msb3 and Msb4 with Cdc42 and polarisome components. Mol Cell Biol. 2005;25:8567-80 pubmed
    ..Here we show that the Rab GTPase-activating proteins (GAPs) Msb3 and Msb4 interact directly with Spa2, a scaffold protein of the "polarisome" that also interacts with the ..
  2. Bi E, Chiavetta J, Chen H, Chen G, Chan C, Pringle J. Identification of novel, evolutionarily conserved Cdc42p-interacting proteins and of redundant pathways linking Cdc24p and Cdc42p to actin polarization in yeast. Mol Biol Cell. 2000;11:773-93 pubmed
    ..targets of Cdc24p instead identified two previously known genes, MSB1 and CLA4, and one novel gene, designated MSB3, all of which appear to function in the Cdc24p-Cdc42p pathway...
  3. Lachmann J, Barr F, Ungermann C. The Msb3/Gyp3 GAP controls the activity of the Rab GTPases Vps21 and Ypt7 at endosomes and vacuoles. Mol Biol Cell. 2012;23:2516-26 pubmed publisher
    ..Here we identify Msb3/Gyp3 as a specific Vps21 GTPase-activating protein (GAP)...
  4. Nickerson D, Russell M, Lo S, Chapin H, Milnes J, Merz A. Termination of isoform-selective Vps21/Rab5 signaling at endolysosomal organelles by Msb3/Gyp3. Traffic. 2012;13:1411-1428 pubmed publisher
    ..The requirement for Rab5 activity in stress tolerance facilitated identification of Msb3/Gyp3 as the principal Rab5 GAP (GTPase accelerating protein)...
  5. Gao X, Albert S, Tcheperegine S, Burd C, Gallwitz D, Bi E. The GAP activity of Msb3p and Msb4p for the Rab GTPase Sec4p is required for efficient exocytosis and actin organization. J Cell Biol. 2003;162:635-46 pubmed
    ..Using a strain defective in polarized secretion and actin-patch organization, we showed that a change in actin-patch organization could be a consequence of the fusion of mistargeted vesicles with the plasma membrane. ..
  6. Albert S, Gallwitz D. Two new members of a family of Ypt/Rab GTPase activating proteins. Promiscuity of substrate recognition. J Biol Chem. 1999;274:33186-9 pubmed
    ..Mdr1/Gyp2p is an efficient GAP for Ypt6p and Sec4p, whereas Msb3/Gyp3p is a potent GAP for Sec4p, Ypt6p, Ypt51p, Ypt31/Ypt32p, and Ypt1p...
  7. Albert S, Gallwitz D. Msb4p, a protein involved in Cdc42p-dependent organization of the actin cytoskeleton, is a Ypt/Rab-specific GAP. Biol Chem. 2000;381:453-6 pubmed
    ..Purified Msb4p/Gyp4p acts primarily on Sec4p, Ypt6p and Ypt7p and might have a role in polarized secretion. ..
  8. Bizimungu C, Vandenbol M. At least two regions of the oncoprotein Tre2 are involved in its lack of GAP activity. Biochem Biophys Res Commun. 2005;335:883-90 pubmed
    ..These complementation experiments revealed, in addition to the TBC domain, a second Tre2 region involved in the oncoprotein's lack of GAP activity: a 93-aa region flanking the TBC domain on the C-terminal side. ..
  9. Dechamps C, Portetelle D, Vandenbol M. Multicopy suppression screen in the msb3 msb4 Saccharomyces cerevisiae double mutant, affected in Ypt/RabGAP activity. Biotechnol Lett. 2005;27:1439-49 pubmed
    ..The msb3 msb4 yeast double mutation causes growth inhibition in the presence of DMSO and/or caffeine, affects the ..

More Information


  1. John Peter A, Lachmann J, Rana M, Bunge M, Cabrera M, Ungermann C. The BLOC-1 complex promotes endosomal maturation by recruiting the Rab5 GTPase-activating protein Msb3. J Cell Biol. 2013;201:97-111 pubmed publisher
    ..Additionally, our data show that BLOC-1 is both a Vps21 effector and an adapter for its GAP Msb3. BLOC-1 and Msb3 interacted in vivo, and both mutants resulted in a redistribution of active Vps21 to the vacuole ..
  2. Li X, Ferro Novick S, Novick P. Different polarisome components play distinct roles in Slt2p-regulated cortical ER inheritance in Saccharomyces cerevisiae. Mol Biol Cell. 2013;24:3145-54 pubmed publisher
    ..Our results point to a common mechanism for the regulation of ER inheritance in which Slt2p activity at the bud tip controls the association of the ER with the actin-based cytoskeleton...
  3. Biver S, Portetelle D, Vandenbol M. Multicopy suppression screen in a Saccharomyces cerevisiae strain lacking the Rab GTPase-activating protein Msb3p. Biotechnol Lett. 2011;33:123-9 pubmed publisher
    ..To see if Msb3p might play an additional, specific role, we first tested the sensitivities of msb3 and msb4 mutant strains to different drugs and then screened a genomic library for multicopy suppressors of msb3 ..
  4. Iwanejko L, Smith K, Loeillet S, Nicolas A, Fabre F. Disruption and functional analysis of six ORFs on chromosome XV: YOL117w, YOL115w ( TRF4), YOL114c, YOL112w ( MSB4), YOL111c and YOL072w. Yeast. 1999;15:1529-39 pubmed
    ..Mutants disrupted for YOL072w, the predicted product of which is unrelated to any protein of known function, grow slowly, are cold-sensitive and sporulate with reduced efficiency. ..
  5. Rana M, Lachmann J, Ungermann C. Identification of a Rab GTPase-activating protein cascade that controls recycling of the Rab5 GTPase Vps21 from the vacuole. Mol Biol Cell. 2015;26:2535-49 pubmed publisher
    ..BLOC-1 complex as an effector of the yeast Rab5 Vps21, which also recruits the GTPase-activating protein (GAP) Msb3. This raises the question of when Vps21 is inactivated on endosomes...
  6. Mayordomo I, Sanz P. The Saccharomyces cerevisiae 14-3-3 protein Bmh2 is required for regulation of the phosphorylation status of Fin1, a novel intermediate filament protein. Biochem J. 2002;365:51-6 pubmed
    ..We also identified components of the vesicular transport machinery such as Gic2 and Msb3, proteins involved in transcriptional regulation such as Mbf1, Gcr2 and Reg2, and a variety of other different ..
  7. Bach S, Bouchat O, Portetelle D, Vandenbol M. Co-deletion of the MSB3 and MSB4 coding regions affects bipolar budding and perturbs the organization of the actin cytoskeleton. Yeast. 2000;16:1015-23 pubmed
    ..the cellular functions of these proteins, we constructed single and double yeast mutants by disrupting the MSB3 gene, the MSB4 gene, or both...
  8. Kozminski K, Alfaro G, Dighe S, Beh C. Homologues of oxysterol-binding proteins affect Cdc42p- and Rho1p-mediated cell polarization in Saccharomyces cerevisiae. Traffic. 2006;7:1224-42 pubmed
    ..Disruption of all OSH gene function caused specific defects in polarized exocytosis, indicating that the Osh proteins are collectively required for a secretory pathway implicated in the maintenance of polarized growth. ..
  9. Zanolari B, Rockenbauch U, Trautwein M, Clay L, Barral Y, Spang A. Transport to the plasma membrane is regulated differently early and late in the cell cycle in Saccharomyces cerevisiae. J Cell Sci. 2011;124:1055-66 pubmed publisher
    ..We propose that transport to and from the bud neck is regulated differently in small- and large-budded cells, and differs early and late in the cell cycle. ..
  10. Bizimungu C, De Neve N, Burny A, Bach S, Bontemps F, Portetelle D, et al. Expression in a RabGAP yeast mutant of two human homologues, one of which is an oncogene. Biochem Biophys Res Commun. 2003;310:498-504 pubmed
    ..the biochemical function of Tre2 oncogene, we expressed its cDNA and, as a control, the RN-tre cDNA, in an msb3 msb4 double mutant yeast strain...