Gene Symbol: MSB2
Description: Msb2p
Alias: Msb2p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Cullen P, Sabbagh W, Graham E, Irick M, van Olden E, Neal C, et al. A signaling mucin at the head of the Cdc42- and MAPK-dependent filamentous growth pathway in yeast. Genes Dev. 2004;18:1695-708 pubmed
    ..This factor, called Msb2, is a FG-pathway-specific factor that promotes differential activation of the MAPK for the FG pathway, Kss1...
  2. Vadaie N, Dionne H, Akajagbor D, Nickerson S, Krysan D, Cullen P. Cleavage of the signaling mucin Msb2 by the aspartyl protease Yps1 is required for MAPK activation in yeast. J Cell Biol. 2008;181:1073-81 pubmed publisher
    ..We found that the Saccharomyces cerevisiae mucin Msb2p, which functions at the head of the Cdc42p-dependent MAPK pathway that controls filamentous growth, is processed ..
  3. Bender A, Pringle J. A Ser/Thr-rich multicopy suppressor of a cdc24 bud emergence defect. Yeast. 1992;8:315-23 pubmed
    ..Its Ser+Thr-richness and hydrophobicity profile suggest that Msb2p may be an integral membrane protein containing a long, periplasmic, N-terminal domain and a short, cytoplasmic, C-..
  4. O Rourke S, Herskowitz I. A third osmosensing branch in Saccharomyces cerevisiae requires the Msb2 protein and functions in parallel with the Sho1 branch. Mol Cell Biol. 2002;22:4739-49 pubmed
    ..We identified the MSB2 gene, which encodes a protein with a single membrane-spanning domain and a large presumptive extracellular domain...
  5. Tatebayashi K, Tanaka K, Yang H, Yamamoto K, Matsushita Y, Tomida T, et al. Transmembrane mucins Hkr1 and Msb2 are putative osmosensors in the SHO1 branch of yeast HOG pathway. EMBO J. 2007;26:3521-33 pubmed
    ..Here, we show that the mucin-like transmembrane proteins Hkr1 and Msb2 are the potential osmosensors for the SHO1 branch...
  6. Abdullah U, Cullen P. The tRNA modification complex elongator regulates the Cdc42-dependent mitogen-activated protein kinase pathway that controls filamentous growth in yeast. Eukaryot Cell. 2009;8:1362-72 pubmed publisher
    ..Genetic suppression analysis showed that elongator functions at the level of Msb2p, the signaling mucin that operates at the head of the pathway, which led to the finding that elongator regulates ..
  7. Chavel C, Dionne H, Birkaya B, Joshi J, Cullen P. Multiple signals converge on a differentiation MAPK pathway. PLoS Genet. 2010;6:e1000883 pubmed publisher
    ..used a novel screening approach (called secretion profiling) that measures release of the extracellular domain of Msb2p, the signaling mucin which functions at the head of the filamentous growth (FG) MAPK pathway...
  8. Adhikari H, Cullen P. Role of phosphatidylinositol phosphate signaling in the regulation of the filamentous-growth mitogen-activated protein kinase pathway. Eukaryot Cell. 2015;14:427-40 pubmed publisher
    ..MAPK regulatory proteins that function at the PM, including Msb2p, Sho1p, and Cdc42p, were mislocalized in the pik1-83 mutant, which may account for the signaling defects of the PI(..
  9. Zuzuarregui A, Li T, Friedmann C, Ammerer G, Alepuz P. Msb2 is a Ste11 membrane concentrator required for full activation of the HOG pathway. Biochim Biophys Acta. 2015;1849:722-30 pubmed publisher
    ..interaction profile during acute stress exposure, with an emphasis on the sensory system of the so-called Sho1/Msb2 signaling branch...

More Information


  1. Bender A, Pringle J. Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the ras-related gene RSR1. Proc Natl Acad Sci U S A. 1989;86:9976-80 pubmed
    ..However, one of the other genes that was isolated by virtue of its ability to suppress cdc24 can also suppress cdc42. This gene, named MSB1, maps between ADE9 and HIS3 on the right arm of chromosome XV. ..
  2. Rodríguez Peña J, Diez Muñiz S, Bermejo C, Nombela C, Arroyo J. Activation of the yeast cell wall integrity MAPK pathway by zymolyase depends on protease and glucanase activities and requires the mucin-like protein Hkr1 but not Msb2. FEBS Lett. 2013;587:3675-80 pubmed publisher
    ..Moreover, we show that the osmosensor Hkr1 is required for signaling, being the most upstream element identified to date. ..
  3. Lien E, Nagiec M, Dohlman H. Proper protein glycosylation promotes mitogen-activated protein kinase signal fidelity. Biochemistry. 2013;52:115-24 pubmed publisher
    ..Deletion of the cell surface mucin Msb2 suppressed the mnn11? phenotype, while mutation of a single glycosylation site within Msb2 was sufficient to confer ..
  4. Adhikari H, Caccamise L, Pande T, Cullen P. Comparative Analysis of Transmembrane Regulators of the Filamentous Growth Mitogen-Activated Protein Kinase Pathway Uncovers Functional and Regulatory Differences. Eukaryot Cell. 2015;14:868-83 pubmed publisher
    ..Several transmembrane (TM) proteins regulate the filamentous growth pathway, including the signaling mucin Msb2p, the tetraspan osmosensor Sho1p, and an adaptor Opy2p...
  5. Karunanithi S, Joshi J, Chavel C, Birkaya B, Grell L, Cullen P. Regulation of mat responses by a differentiation MAPK pathway in Saccharomyces cerevisiae. PLoS ONE. 2012;7:e32294 pubmed publisher
    ..Signaling pathways may regulate similar processes in pathogens, whose highly nuanced responses are required for virulence. ..
  6. Yamamoto K, Tatebayashi K, Tanaka K, Saito H. Dynamic control of yeast MAP kinase network by induced association and dissociation between the Ste50 scaffold and the Opy2 membrane anchor. Mol Cell. 2010;40:87-98 pubmed publisher
    ..Thus, dynamic regulation of Ste50-Opy2 interaction fine-tunes the MAPK signaling network. ..
  7. Pitoniak A, Birkaya B, Dionne H, Vadaie N, Cullen P. The signaling mucins Msb2 and Hkr1 differentially regulate the filamentation mitogen-activated protein kinase pathway and contribute to a multimodal response. Mol Biol Cell. 2009;20:3101-14 pubmed publisher
    ..FG pathway activity was modulated by the HOG pathway, which led to the finding that the signaling mucins Msb2p and Hkr1p, which operate at the head of the HOG pathway, differentially regulate the FG pathway...
  8. Yang H, Tatebayashi K, Yamamoto K, Saito H. Glycosylation defects activate filamentous growth Kss1 MAPK and inhibit osmoregulatory Hog1 MAPK. EMBO J. 2009;28:1380-91 pubmed publisher
    ..The O-glycosylated membrane proteins Msb2 and Opy2 are both essential for activating the FG MAPK pathway, but only defective glycosylation of Msb2 activates ..
  9. Hirschmann W, Westendorf H, Mayer A, Cannarozzi G, Cramer P, Jansen R. Scp160p is required for translational efficiency of codon-optimized mRNAs in yeast. Nucleic Acids Res. 2014;42:4043-55 pubmed publisher
    ..Our data indicate that Scp160p might increase the efficiency of tRNA recharge, or prevent diffusion of discharged tRNAs, both of which were also proposed to be the likely basis for the translational fitness effect of tRNA pairing...
  10. Tanaka K, Tatebayashi K, Nishimura A, Yamamoto K, Yang H, Saito H. Yeast osmosensors Hkr1 and Msb2 activate the Hog1 MAPK cascade by different mechanisms. Sci Signal. 2014;7:ra21 pubmed publisher
    ..Furthermore, the Sho1 signaling branch itself also involves the two functionally redundant osmosensors Hkr1 and Msb2. However, any single osmosensor (Sln1, Hkr1, or Msb2) is sufficient for osmoadaptation...
  11. Adhikari H, Vadaie N, Chow J, Caccamise L, Chavel C, Li B, et al. Role of the unfolded protein response in regulating the mucin-dependent filamentous-growth mitogen-activated protein kinase pathway. Mol Cell Biol. 2015;35:1414-32 pubmed publisher
    Signaling mucins are evolutionarily conserved regulators of signal transduction pathways. The signaling mucin Msb2p regulates the Cdc42p-dependent mitogen-activated protein kinase (MAPK) pathway that controls filamentous growth in yeast...
  12. Yamamoto K, Tatebayashi K, Saito H. Binding of the Extracellular Eight-Cysteine Motif of Opy2 to the Putative Osmosensor Msb2 Is Essential for Activation of the Yeast High-Osmolarity Glycerol Pathway. Mol Cell Biol. 2016;36:475-87 pubmed publisher that the extracellular cysteine-rich (CR) domain of the transmembrane-anchor protein Opy2 binds to the Hkr1-Msb2 homology (HMH) domain of the putative osmosensor Msb2 and that formation of the Opy2-Msb2 complex is essential for ..
  13. Li S, Diakov T, Rizzo J, Kane P. Vacuolar H+-ATPase works in parallel with the HOG pathway to adapt Saccharomyces cerevisiae cells to osmotic stress. Eukaryot Cell. 2012;11:282-91 pubmed publisher
    ..Together, these data suggest that the V-ATPase acts in parallel with the HOG pathway in order to mediate salt detoxification. ..
  14. Karunanithi S, Cullen P. The filamentous growth MAPK Pathway Responds to Glucose Starvation Through the Mig1/2 transcriptional repressors in Saccharomyces cerevisiae. Genetics. 2012;192:869-87 pubmed publisher
    ..with other regulators of the filamentous growth pathway including the cytosolic domain of the signaling mucin Msb2, the MAP kinase kinase Ste7, and the MAP kinase Kss1...
  15. Gandhi M, Goode B, Chan C. Four novel suppressors of gic1 gic2 and their roles in cytokinesis and polarized cell growth in Saccharomyces cerevisiae. Genetics. 2006;174:665-78 pubmed
    ..Our analysis of the second pair of gic1 gic2 suppressors, VHS2 and MLF3, suggests that they regulate polarization of the actin cytoskeleton and cell growth and function in a pathway distinct from and parallel to GIC1 and GIC2. ..
  16. Basu S, Vadaie N, Prabhakar A, Li B, Adhikari H, Pitoniak A, et al. Spatial landmarks regulate a Cdc42-dependent MAPK pathway to control differentiation and the response to positional compromise. Proc Natl Acad Sci U S A. 2016;113:E2019-28 pubmed publisher
    ..Therefore, a surveillance mechanism monitors spatial position in response to extrinsic and intrinsic stress and modulates the response through a differentiation MAPK pathway. ..