Gene Symbol: MRS4
Description: Fe(2+) transporter
Alias: Fe(2+) transporter
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Li L, Kaplan J. A mitochondrial-vacuolar signaling pathway in yeast that affects iron and copper metabolism. J Biol Chem. 2004;279:33653-61 pubmed
    ..Overexpression of MRS3 or MRS4 suppresses the high iron sensitivity of Deltaccc1 cells...
  2. Froschauer E, Schweyen R, Wiesenberger G. The yeast mitochondrial carrier proteins Mrs3p/Mrs4p mediate iron transport across the inner mitochondrial membrane. Biochim Biophys Acta. 2009;1788:1044-50 pubmed publisher
    ..We conclude that the carrier proteins Mrs3p and Mrs4p transport Fe(2+) across the inner mitochondrial membrane. Their activity is dependent on the pH gradient and it is stimulated by iron shortage. ..
  3. Muhlenhoff U, Stadler J, Richhardt N, Seubert A, Eickhorst T, Schweyen R, et al. A specific role of the yeast mitochondrial carriers MRS3/4p in mitochondrial iron acquisition under iron-limiting conditions. J Biol Chem. 2003;278:40612-20 pubmed
    The yeast genes MRS3 and MRS4 encode two members of the mitochondrial carrier family with high sequence similarity...
  4. Li L, Miao R, Jia X, Ward D, Kaplan J. Expression of the yeast cation diffusion facilitators Mmt1 and Mmt2 affects mitochondrial and cellular iron homeostasis: evidence for mitochondrial iron export. J Biol Chem. 2014;289:17132-41 pubmed publisher
    ..The phenotypes due to overexpression of MMT1&2 were similar to that seen in cells with deletions in MRS3 and MRS4, genes that encode the mitochondrial iron importers...
  5. Farooq M, Pracheil T, Dong Z, Xiao F, Liu Z. Mitochondrial DNA instability in cells lacking aconitase correlates with iron citrate toxicity. Oxid Med Cell Longev. 2013;2013:493536 pubmed publisher
    ..Increased activity of citrate synthase leads to iron accumulation in the mitochondria. Mutations in MRS3 and MRS4, encoding two mitochondrial iron transporters, also prevented mtDNA loss due to aco1?...
  6. Zhang Y, Lyver E, Nakamaru Ogiso E, Yoon H, Amutha B, Lee D, et al. Dre2, a conserved eukaryotic Fe/S cluster protein, functions in cytosolic Fe/S protein biogenesis. Mol Cell Biol. 2008;28:5569-82 pubmed publisher
    ..mutation of the essential DRE2 gene was found to confer lethality when combined with Delta mrs3 and Delta mrs4. The dre2 mutant or Dre2-depleted cells were deficient in cytosolic Fe/S cluster protein activities while ..
  7. Li L, Murdock G, Bagley D, Jia X, Ward D, Kaplan J. Genetic dissection of a mitochondria-vacuole signaling pathway in yeast reveals a link between chronic oxidative stress and vacuolar iron transport. J Biol Chem. 2010;285:10232-42 pubmed publisher
    Deletion of two homologous genes, MRS3 and MRS4, that encode mitochondrial iron transporters affects the activity of the vacuolar iron importer Ccc1...
  8. Zhang Y, Lyver E, Knight S, Pain D, Lesuisse E, Dancis A. Mrs3p, Mrs4p, and frataxin provide iron for Fe-S cluster synthesis in mitochondria. J Biol Chem. 2006;281:22493-502 pubmed
    ..These findings show that Mrs3/4p transports iron into mitochondria, whereas frataxin makes iron already within mitochondria available for Fe-S cluster synthesis. ..
  9. Gregan J, Kolisek M, Schweyen R. Mitochondrial Mg(2+) homeostasis is critical for group II intron splicing in vivo. Genes Dev. 2001;15:2229-37 pubmed
    ..It explains finally why protein factors modulating Mg(2+) homeostasis had been identified in genetic screens for bona fide RNA splicing factors. ..

More Information


  1. Wiesenberger G, Link T, von Ahsen U, Waldherr M, Schweyen R. MRS3 and MRS4, two suppressors of mtRNA splicing defects in yeast, are new members of the mitochondrial carrier family. J Mol Biol. 1991;217:23-37 pubmed
    When present in high copy number plasmids, the nuclear genes MRS3 and MRS4 from Saccharomyces cerevisiae can suppress the mitochondrial RNA splicing defects of several mit- intron mutations...
  2. Lin H, Li L, Jia X, Ward D, Kaplan J. Genetic and biochemical analysis of high iron toxicity in yeast: iron toxicity is due to the accumulation of cytosolic iron and occurs under both aerobic and anaerobic conditions. J Biol Chem. 2011;286:3851-62 pubmed publisher aerobically grown ?ccc1 cells encode organelle iron transporters including mitochondrial iron transporters MRS3, MRS4, and RIM2...
  3. Yoon H, Zhang Y, Pain J, Lyver E, Lesuisse E, Pain D, et al. Rim2, a pyrimidine nucleotide exchanger, is needed for iron utilization in mitochondria. Biochem J. 2011;440:137-46 pubmed publisher
    ..screen looking for yeast mutants that are synthetically lethal with the mitochondrial iron carriers Mrs3 and Mrs4. Several genes were identified, including an isolate mutated for Yfh1, the yeast frataxin homologue...
  4. Froschauer E, Rietzschel N, Hassler M, Binder M, Schweyen R, Lill R, et al. The mitochondrial carrier Rim2 co-imports pyrimidine nucleotides and iron. Biochem J. 2013;455:57-65 pubmed publisher
    ..The mitochondrial carrier family members Mrs3 and Mrs4 (homologues of vertebrate mitoferrin) function in organellar iron supply, yet other low efficiency transporters may ..
  5. Brazzolotto X, Pierrel F, Pelosi L. Three conserved histidine residues contribute to mitochondrial iron transport through mitoferrins. Biochem J. 2014;460:79-89 pubmed publisher
    ..A model describing how these residues help iron to transit smoothly across the carrier cavity is proposed and compared with the structural and biochemical data available for other carriers in this family. ..
  6. Shaw G, Cope J, Li L, Corson K, Hersey C, Ackermann G, et al. Mitoferrin is essential for erythroid iron assimilation. Nature. 2006;440:96-100 pubmed
    ..Disruption of the yeast mfrn orthologues, MRS3 and MRS4, causes defects in iron metabolism and mitochondrial Fe-S cluster biogenesis...
  7. Zhang Y, Lyver E, Knight S, Lesuisse E, Dancis A. Frataxin and mitochondrial carrier proteins, Mrs3p and Mrs4p, cooperate in providing iron for heme synthesis. J Biol Chem. 2005;280:19794-807 pubmed
    ..Deletion of the yeast frataxin homolog (YFH1) was combined with deletions of MRS3 and MRS4, mitochondrial carrier proteins implicated in iron homeostasis...
  8. Waldherr M, Ragnini A, Jank B, Teply R, Wiesenberger G, Schweyen R. A multitude of suppressors of group II intron-splicing defects in yeast. Curr Genet. 1993;24:301-6 pubmed
    ..MRS3 and MRS4, the genes for two mitochondrial-solute carrier proteins, can suppress both phenotypes when present in high-copy-..