Gene Symbol: MRF1
Species: Saccharomyces cerevisiae S288c
Heurgué Hamard V, Champ S, Mora L, Merkulova Rainon T, Merkoulova Rainon T, Kisselev L, et al
. The glutamine residue of the conserved GGQ motif in Saccharomyces cerevisiae release factor eRF1 is methylated by the product of the YDR140w gene. J Biol Chem. 2005;280:2439-45 pubmed
Pel H, Maat C, Rep M, Grivell L. The yeast nuclear gene MRF1 encodes a mitochondrial peptide chain release factor and cures several mitochondrial RNA splicing defects. Nucleic Acids Res. 1992;20:6339-46 pubmed
..and genetic characterization of the first gene encoding an organellar polypeptide chain release factor, the MRF1 gene of the yeast Saccharomyces cerevisiae...
Claisse M, Boguta M, Zagórski W. Translational readthrough of a termination codon in the yeast mitochondrial mRNA VAR1 as a result of mutation in the release factor mRF1. Acta Biochim Pol. 2005;52:129-37 pubmed
..Translation of he mitochondrially encoded polypeptides in strains with mutated mitochondrial release factor, mRF1, was found to result in the synthesis of a novel protein, V2...
Askarian Amiri M, Pel H, Guevremont D, McCaughan K, Poole E, Sumpter V, et al
. Functional characterization of yeast mitochondrial release factor 1. J Biol Chem. 2000;275:17241-8 pubmed
The yeast Saccharomyces cerevisiae mitochondrial release factor was expressed from the cloned MRF1 gene, purified from inclusion bodies, and refolded to give functional activity...
Grandier Vazeille X, Bathany K, Chaignepain S, Camougrand N, Manon S, Schmitter J. Yeast mitochondrial dehydrogenases are associated in a supramolecular complex. Biochemistry. 2001;40:9758-69 pubmed
..The association of these proteins is discussed in terms of NADH-channeling. ..
Towpik J, Chacinska A, Ciesla M, Ginalski K, Boguta M. Mutations in the yeast mrf1 gene encoding mitochondrial release factor inhibit translation on mitochondrial ribosomes. J Biol Chem. 2004;279:14096-103 pubmed
..stop codons in mitochondrial genes were localized in the chromosomal gene encoding the mitochondrial release factor mRF1. The mrf1-13 and mrf1-780 mutant genes, in contrast to other alleles, caused a non-respiratory phenotype that ..
Towpik J, Kutner J, Boguta M. Expression of mitochondrial release factor in relation to respiratory competence in yeast. Curr Genet. 2005;48:101-8 pubmed
..The yeast mitochondrial release factor, mRF1, is a protein of 43 kDa that emerges from its precursor by cleavage of a mitochondrial targeting sequence...
Polevoda B, Span L, Sherman F. The yeast translation release factors Mrf1p and Sup45p (eRF1) are methylated, respectively, by the methyltransferases Mtq1p and Mtq2p. J Biol Chem. 2006;281:2562-71 pubmed
..The Saccharomyces cerevisiae RFs, mitochondrial Mrf1p and cytoplasmic Sup45p (eRF1), have sequence similarities to the bacterial RFs, including the potential site of ..
Pel H, Rep M, Dubbink H, Grivell L. Single point mutations in domain II of the yeast mitochondrial release factor mRF-1 affect ribosome binding. Nucleic Acids Res. 1993;21:5308-15 pubmed
We have recently described two yeast strains that are mutated in the MRF1 gene encoding the mitochondrial release factor mRF-1. Both mutants provoke gene-specific defects in mitochondrial translational termination...