Gene Symbol: MPT5
Description: Mpt5p
Alias: HTR1, PUF5, UTH4, Mpt5p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Kennedy B, Gotta M, Sinclair D, Mills K, McNabb D, Murthy M, et al. Redistribution of silencing proteins from telomeres to the nucleolus is associated with extension of life span in S. cerevisiae. Cell. 1997;89:381-91 pubmed
    ..Furthermore, this relocalization is dependent on both UTH4 a novel yeast gene that extends life span, and its homologue YGL023...
  2. Kaeberlein M, Kirkland K, Fields S, Kennedy B. Genes determining yeast replicative life span in a long-lived genetic background. Mech Ageing Dev. 2005;126:491-504 pubmed
    ..These results define a limited number of genes likely to regulate replicative life span in a strain-independent manner, and create a basis for future epistasis analysis to determine genetic pathways of aging. ..
  3. Hook B, Goldstrohm A, Seay D, Wickens M. Two yeast PUF proteins negatively regulate a single mRNA. J Biol Chem. 2007;282:15430-8 pubmed Saccharomyces cerevisiae, encoding the HO endonuclease, is regulated by two distinct PUF proteins, Puf4p and Mpt5p. These proteins bind to adjacent sites and can co-occupy the mRNA...
  4. Hata H, Mitsui H, Liu H, Bai Y, Denis C, Shimizu Y, et al. Dhh1p, a putative RNA helicase, associates with the general transcription factors Pop2p and Ccr4p from Saccharomyces cerevisiae. Genetics. 1998;148:571-9 pubmed
    ..five multicopy suppressor genes of a pop2 deletion mutation: CCR4, DHH1 (a putative RNA helicase), PKC1, STM1, and MPT5 (multicopy suppressor of pop two)...
  5. Prinz S, Aldridge C, Ramsey S, Taylor R, Galitski T. Control of signaling in a MAP-kinase pathway by an RNA-binding protein. PLoS ONE. 2007;2:e249 pubmed
    ..Here we show that a PUF-family RNA-binding protein, Mpt5, represses the yeast MAP-kinase pathway controlling differentiation to the filamentous form...
  6. Kikuchi Y, Oka Y, Kobayashi M, Uesono Y, Toh e A, Kikuchi A. A new yeast gene, HTR1, required for growth at high temperature, is needed for recovery from mating pheromone-induced G1 arrest. Mol Gen Genet. 1994;245:107-16 pubmed
    ..A DNA fragment was cloned based on its ability to complement this temperature sensitivity. The HTR1 gene encodes a putative protein of 93 kDa without significant homology to any known proteins...
  7. Chen T, Kurjan J. Saccharomyces cerevisiae Mpt5p interacts with Sst2p and plays roles in pheromone sensitivity and recovery from pheromone arrest. Mol Cell Biol. 1997;17:3429-39 pubmed
    ..We have identified an interaction between Sst2p and the previously identified Mpt5p by using the two-hybrid system...
  8. Ulbricht R, Olivas W. Puf1p acts in combination with other yeast Puf proteins to control mRNA stability. RNA. 2008;14:246-62 pubmed
  9. Chritton J, Wickens M. Translational repression by PUF proteins in vitro. RNA. 2010;16:1217-25 pubmed publisher
    ..We suggest a model in which PUF proteins can control translation termination or elongation. ..

More Information


  1. Kaeberlein M, Guarente L. Saccharomyces cerevisiae MPT5 and SSD1 function in parallel pathways to promote cell wall integrity. Genetics. 2002;160:83-95 pubmed
    ..We show here that MPT5 also functions to promote cell wall integrity. Loss of Mpt5p results in phenotypes associated with a weakened cell wall, including sorbitol-remedial temperature sensitivity ..
  2. Olivas W, Parker R. The Puf3 protein is a transcript-specific regulator of mRNA degradation in yeast. EMBO J. 2000;19:6602-11 pubmed
    ..of five members of the Puf family present in the yeast genome (referred to as JSN1/PUF1, PUF2, PUF3, PUF4 and MPT5/PUF5). Yeast strains lacking all five Puf proteins showed differential expression of numerous yeast mRNAs...
  3. Tadauchi T, Matsumoto K, Herskowitz I, Irie K. Post-transcriptional regulation through the HO 3'-UTR by Mpt5, a yeast homolog of Pumilio and FBF. EMBO J. 2001;20:552-61 pubmed
    ..elegans, humans, and yeasts. Budding yeast, Saccharomyces cerevisiae, has five proteins with conserved Puf motifs: Mpt5/Uth4, Ygl014w, Yll013c, Jsn1, and Ypr042c. Here we report that Mpt5 negatively regulates expression of the HO gene...
  4. Stewart M, Krause S, McGhie J, Gray J. Mpt5p, a stress tolerance- and lifespan-promoting PUF protein in Saccharomyces cerevisiae, acts upstream of the cell wall integrity pathway. Eukaryot Cell. 2007;6:262-70 pubmed
    ..The PUF domain is required and sufficient for this function. One Saccharomyces cerevisiae PUF protein, Mpt5p (also called Puf5p or Uth4p), promotes stress tolerance and replicative life span (the maximum number of doublings ..
  5. Xu B, Skowronek K, Kurjan J. The N terminus of Saccharomyces cerevisiae Sst2p plays an RGS-domain-independent, Mpt5p-dependent role in recovery from pheromone arrest. Genetics. 2001;159:1559-71 pubmed
    ..Other results indicate that Sst2p acts through Mpt5p and that this action occurs downstream of Fus3p and through Cln3p/Cdc28p...
  6. Goldstrohm A, Hook B, Seay D, Wickens M. PUF proteins bind Pop2p to regulate messenger RNAs. Nat Struct Mol Biol. 2006;13:533-9 pubmed
    ..We suggest that the PUF-Pop2p interaction underlies regulated deadenylation, mRNA decay and repression by PUF proteins. ..
  7. Russo J, Olivas W. Conditional regulation of Puf1p, Puf4p, and Puf5p activity alters YHB1 mRNA stability for a rapid response to toxic nitric oxide stress in yeast. Mol Biol Cell. 2015;26:1015-29 pubmed publisher
    ..Puf1p and Puf4p can bind target mRNA in inactivating conditions; however, Puf5p binding is reduced. This work demonstrates how multiple Puf proteins coordinately regulate YHB1 mRNA to protect cells from nitric oxide stress. ..
  8. Li L, Miles S, Melville Z, Prasad A, Bradley G, Breeden L. Key events during the transition from rapid growth to quiescence in budding yeast require posttranscriptional regulators. Mol Biol Cell. 2013;24:3697-709 pubmed publisher
    ..Ssd1 and the related mRNA-binding protein Mpt5 play critical overlapping roles in Q-cell formation and longevity...
  9. Fujii M, Miki K, Takayama S, Ayusawa D. Identification of genes that affect sensitivity to 5-bromodeoxyuridine in the yeast Saccharomyces cerevisiae. Mol Genet Genomics. 2010;283:461-8 pubmed publisher
    ..Our genetic screening has revealed that a defect in MPT5/HTR1/UTH4/PUF5 led to an increased sensitivity to BrdU, and that overexpression of VHT1 or SDT1 led to resistance to BrdU...
  10. Cridge A, Castelli L, Smirnova J, Selley J, Rowe W, Hubbard S, et al. Identifying eIF4E-binding protein translationally-controlled transcripts reveals links to mRNAs bound by specific PUF proteins. Nucleic Acids Res. 2010;38:8039-50 pubmed publisher
    ..Thus the combined action of each 4E-BP with specific 3'-UTR-binding proteins mediates mRNA-specific translational control in yeast, showing that this form of translational control is more widely employed than previously thought. ..
  11. Takeuchi J, Okada M, Toh e A, Kikuchi Y. The SMS1 gene encoding a serine-rich transmembrane protein suppresses the temperature sensitivity of the htr1 disruptant in Saccharomyces cerevisiae. Biochim Biophys Acta. 1995;1260:94-6 pubmed
    ..suppressor) of Saccharomyces cerevisiae was isolated by the ability to suppress the temperature sensitivity of the htr1 disruptant (Kikuchi et al. (1994) Mol. Gen. Genetics, in press) by increasing its gene dosage...
  12. Traven A, Lo T, Lithgow T, Heierhorst J. The yeast PUF protein Puf5 has Pop2-independent roles in response to DNA replication stress. PLoS ONE. 2010;5:e10651 pubmed publisher
    PUFs are RNA binding proteins that promote mRNA deadenylation and decay and inhibit translation. Yeast Puf5 is the prototype for studying PUF-dependent gene repression...
  13. Bourens M, Panozzo C, Nowacka A, Imbeaud S, Mucchielli M, Herbert C. Mutations in the Saccharomyces cerevisiae kinase Cbk1p lead to a fertility defect that can be suppressed by the absence of Brr1p or Mpt5p (Puf5p), proteins involved in RNA metabolism. Genetics. 2009;183:161-73 pubmed publisher
    ..Among these, mutations in BRR1 or MPT5 lead to a restoration of fertility and a more-or-less pronounced restoration of polarity; they also show genetic ..
  14. Yosefzon Y, Koh Y, Chritton J, Lande A, Leibovich L, Barziv L, et al. Divergent RNA binding specificity of yeast Puf2p. RNA. 2011;17:1479-88 pubmed publisher
    ..This study expands the repertoire of cis elements bound by PUF proteins and suggests new modes by which PUF proteins recognize their mRNA targets. ..
  15. Blewett N, Goldstrohm A. A eukaryotic translation initiation factor 4E-binding protein promotes mRNA decapping and is required for PUF repression. Mol Cell Biol. 2012;32:4181-94 pubmed
    ..The implication of these findings is that a 4E-BP can repress protein expression by promoting specific mRNA degradation steps in addition to or in lieu of inhibiting translation initiation. ..
  16. Utsugi T, Toh e A, Kikuchi Y. A high dose of the STM1 gene suppresses the temperature sensitivity of the tom1 and htr1 mutants in Saccharomyces cerevisiae. Biochim Biophys Acta. 1995;1263:285-8 pubmed
    ..The gene could also suppress the temperature sensitivity of the htr1 disruptant (Kikuchi et al. (1994) Mol. Gen. Genet...
  17. Shimizu Y, Nagai M, Yeasmin A, Koike N, Talukdar M, Ushimaru T. Elucidation of novel budding yeast separase mutants. Biosci Biotechnol Biochem. 2016;80:473-8 pubmed publisher
    ..The Drosophila Pumilio homolog MPT5 was isolated as a high-dosage suppressor of esp1-ts cells...
  18. Wilinski D, Qiu C, Lapointe C, Nevil M, Campbell Z, Tanaka Hall T, et al. RNA regulatory networks diversified through curvature of the PUF protein scaffold. Nat Commun. 2015;6:8213 pubmed publisher
    ..A single PUF protein repeat is sufficient to induce broadening of specificity. Changes in protein architecture, such as alterations in curvature, may lead to evolution of mRNA regulatory networks. ..