MPS2

Summary

Gene Symbol: MPS2
Description: Mps2p
Alias: MMC1, Mps2p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Winey M, Goetsch L, Baum P, Byers B. MPS1 and MPS2: novel yeast genes defining distinct steps of spindle pole body duplication. J Cell Biol. 1991;114:745-54 pubmed
    ..Newly isolated mutations described here (mps1 and mps2, for monopolar spindle) similarly cause monopolar mitosis but their underlying effects on SPB duplication are ..
  2. Muñoz Centeno M, McBratney S, Monterrosa A, Byers B, Mann C, Winey M. Saccharomyces cerevisiae MPS2 encodes a membrane protein localized at the spindle pole body and the nuclear envelope. Mol Biol Cell. 1999;10:2393-406 pubmed
    ..Visualization of a green fluorescent protein (GFP) Mps2p fusion protein in living cells and indirect immunofluorescence microscopy of 9xmyc-Mps2p revealed a perinuclear ..
  3. Jaspersen S, Giddings T, Winey M. Mps3p is a novel component of the yeast spindle pole body that interacts with the yeast centrin homologue Cdc31p. J Cell Biol. 2002;159:945-56 pubmed
  4. Schramm C, Elliott S, Shevchenko A, Schiebel E. The Bbp1p-Mps2p complex connects the SPB to the nuclear envelope and is essential for SPB duplication. EMBO J. 2000;19:421-33 pubmed
    ..This function in SPB duplication is probably fulfilled by a stable complex of Bbp1p and Mps2p, a nuclear envelope protein that is also essential for duplication plaque insertion...
  5. Niepel M, Strambio de Castillia C, Fasolo J, Chait B, Rout M. The nuclear pore complex-associated protein, Mlp2p, binds to the yeast spindle pole body and promotes its efficient assembly. J Cell Biol. 2005;170:225-35 pubmed
    ..Based on these data, we propose that Mlp2p links the SPB to the peripheral Mlp assembly, and that this linkage is required for efficient incorporation of components into the SPB. ..
  6. Araki Y, Lau C, Maekawa H, Jaspersen S, Giddings T, Schiebel E, et al. The Saccharomyces cerevisiae spindle pole body (SPB) component Nbp1p is required for SPB membrane insertion and interacts with the integral membrane proteins Ndc1p and Mps2p. Mol Biol Cell. 2006;17:1959-70 pubmed
    ..Furthermore, Nbp1p is in the Mps2p-Bbp1p complex in the SPB...
  7. Witkin K, Friederichs J, COHEN FIX O, Jaspersen S. Changes in the nuclear envelope environment affect spindle pole body duplication in Saccharomyces cerevisiae. Genetics. 2010;186:867-83 pubmed publisher
    ..We propose a model whereby nuclear pore complexes either compete with the spindle pole body for insertion into the nuclear membrane or affect spindle pole body duplication by altering the nuclear envelope environment. ..
  8. Jaspersen S, Martin A, Glazko G, Giddings T, Morgan G, Mushegian A, et al. The Sad1-UNC-84 homology domain in Mps3 interacts with Mps2 to connect the spindle pole body with the nuclear envelope. J Cell Biol. 2006;174:665-75 pubmed
    ..Integral membrane proteins including Mps2 anchor the soluble core SPB in the nuclear envelope...
  9. Horigome C, Okada T, Shimazu K, Gasser S, Mizuta K. Ribosome biogenesis factors bind a nuclear envelope SUN domain protein to cluster yeast telomeres. EMBO J. 2011;30:3799-811 pubmed publisher
    ..Our results suggest that the ribosome biogenesis factors Ebp2 and Rrs1 cooperate with Mps3 to mediate telomere clustering, but not telomere tethering, by binding Sir4. ..

More Information

Publications24

  1. Loog M, Morgan D. Cyclin specificity in the phosphorylation of cyclin-dependent kinase substrates. Nature. 2005;434:104-8 pubmed
    ..Thus, Clb5 and Clb2 use distinct mechanisms to enhance the phosphorylation of S-phase and M-phase substrates. ..
  2. Sezen B, Seedorf M, Schiebel E. The SESA network links duplication of the yeast centrosome with the protein translation machinery. Genes Dev. 2009;23:1559-70 pubmed publisher
    ..We suggest that the SESA network provides a mechanism by which cells can regulate the translation of specific mRNAs. This regulation is used to coordinate competing events in the nuclear envelope. ..
  3. Hardwick K, Li R, Mistrot C, Chen R, Dann P, Rudner A, et al. Lesions in many different spindle components activate the spindle checkpoint in the budding yeast Saccharomyces cerevisiae. Genetics. 1999;152:509-18 pubmed
    ..In contrast, the cell cycle arrest caused by mutations that induce DNA damage (cdc13), inactivate the cyclin proteolysis machinery (cdc16 and cdc23), or arrest cells in anaphase (cdc15) is independent of the spindle checkpoint. ..
  4. Sezen B. Reduction of Saccharomyces cerevisiae Pom34 protein level by SESA network is related to membrane lipid composition. FEMS Yeast Res. 2015;15: pubmed publisher
    ..Thus, we propose that SESA's action in SPB duplication process is dependent on the alteration of membrane lipid composition to facilitate the insertion process. ..
  5. Li P, Shao Y, Jin H, Yu H. Ndj1, a telomere-associated protein, regulates centrosome separation in budding yeast meiosis. J Cell Biol. 2015;209:247-59 pubmed publisher
    ..These findings reveal the underlying mechanism that coordinates yeast centrosome dynamics with meiotic telomere movement and cell cycle progression. ..
  6. Le Masson I, Yu D, Jensen K, Chevalier A, Courbeyrette R, Boulard Y, et al. Yaf9, a novel NuA4 histone acetyltransferase subunit, is required for the cellular response to spindle stress in yeast. Mol Cell Biol. 2003;23:6086-102 pubmed
    ..These results strongly suggest that acetylation of histone H4 by NuA4 is required for the cellular resistance to spindle stress. ..
  7. McBratney S, Winey M. Mutant membrane protein of the budding yeast spindle pole body is targeted to the endoplasmic reticulum degradation pathway. Genetics. 2002;162:567-78 pubmed
    ..The Mps2-1p mutant protein level is markedly reduced compared to wild-type Mps2p, and deletion of CUE1 restores the level of Mps2-1p to nearly wild-type levels...
  8. Casey A, Dawson T, Chen J, Friederichs J, Jaspersen S, Wente S. Integrity and function of the Saccharomyces cerevisiae spindle pole body depends on connections between the membrane proteins Ndc1, Rtn1, and Yop1. Genetics. 2012;192:441-55 pubmed publisher
    ..We found that overexpression of the SPB insertion factors NDC1, MPS2, or BBP1 rescued the SPB defects observed in rtn1? yop1? cells...
  9. Anderson V, Prudden J, Prochnik S, Giddings T, Hardwick K. Novel sfi1 alleles uncover additional functions for Sfi1p in bipolar spindle assembly and function. Mol Biol Cell. 2007;18:2047-56 pubmed
    ..3 microm apart. Importantly, these SPBs have completed duplication, but they are not separated, suggesting a possible defect in splitting of the bridge. We discuss possible roles for Sfi1p in this step in bipolar spindle assembly. ..
  10. Zizlsperger N, Keating A. Specific coiled-coil interactions contribute to a global model of the structure of the spindle pole body. J Struct Biol. 2010;170:246-56 pubmed publisher
    ..Self-associating coiled coils from Bbp1, Mps2, and Nbp1 were observed to form stable parallel homodimers in solution...
  11. Keck J, Jones M, Wong C, Binkley J, Chen D, Jaspersen S, et al. A cell cycle phosphoproteome of the yeast centrosome. Science. 2011;332:1557-61 pubmed publisher
    ..Our work establishes the extent and complexity of this prominent posttranslational modification in centrosome biology and provides specific examples of phosphorylation control in centrosome function. ..
  12. Le Masson I, Saveanu C, Chevalier A, Namane A, Gobin R, Fromont Racine M, et al. Spc24 interacts with Mps2 and is required for chromosome segregation, but is not implicated in spindle pole body duplication. Mol Microbiol. 2002;43:1431-43 pubmed
    b>Mps2 (monopolar spindle protein) is a coiled-coil protein found at the spindle pole body (SPB) and at the nuclear envelope that is required for insertion of the SPB into the nuclear envelope...
  13. Kupke T, Malsam J, Schiebel E. A ternary membrane protein complex anchors the spindle pole body in the nuclear envelope in budding yeast. J Biol Chem. 2017;292:8447-8458 pubmed publisher
    ..The SPB is built from 18 different proteins, including the three integral membrane proteins Mps3, Ndc1, and Mps2. These membrane proteins play an essential role in the insertion of the new SPB into the NE...
  14. Ravid T, Kreft S, Hochstrasser M. Membrane and soluble substrates of the Doa10 ubiquitin ligase are degraded by distinct pathways. EMBO J. 2006;25:533-43 pubmed
    ..Thus, while Doa10 ubiquitinates both membrane and soluble proteins, the mechanisms of subsequent proteasome targeting differ. ..
  15. Katta S, Chen J, Gardner J, Friederichs J, Smith S, Gogol M, et al. Sec66-Dependent Regulation of Yeast Spindle-Pole Body Duplication Through Pom152. Genetics. 2015;201:1479-95 pubmed publisher
    ..we carried out genome-wide screens for suppressors of deletion alleles of SPB components, including Mps3 and Mps2. In addition to the nucleoporins POM152 and POM34, we found that elimination of SEC66/SEC71/KAR7 suppressed ..