MPH1

Summary

Gene Symbol: MPH1
Description: 3'-5' DNA helicase
Alias: 3'-5' DNA helicase
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Panico E, Ede C, Schildmann M, Schürer K, Kramer W. Genetic evidence for a role of Saccharomyces cerevisiae Mph1 in recombinational DNA repair under replicative stress. Yeast. 2010;27:11-27 pubmed publisher
    ..The Saccharomyces cerevisiae MPH1 gene, homologue of human FANCM, has been involved in homologous recombination and DNA repair...
  2. Scheller J, Schürer A, Rudolph C, Hettwer S, Kramer W. MPH1, a yeast gene encoding a DEAH protein, plays a role in protection of the genome from spontaneous and chemically induced damage. Genetics. 2000;155:1069-81 pubmed
    We have characterized the MPH1 gene from Saccharomyces cerevisiae. mph1 mutants display a spontaneous mutator phenotype. Homologs were found in archaea and in the EST libraries of Drosophila, mouse, and man...
  3. Yan Z, Delannoy M, Ling C, Daee D, Osman F, Muniandy P, et al. A histone-fold complex and FANCM form a conserved DNA-remodeling complex to maintain genome stability. Mol Cell. 2010;37:865-78 pubmed publisher
    ..Thus, FANCM-MHF is an essential DNA-remodeling complex that protects replication forks from yeast to human. ..
  4. Schürer K, Rudolph C, Ulrich H, Kramer W. Yeast MPH1 gene functions in an error-free DNA damage bypass pathway that requires genes from Homologous recombination, but not from postreplicative repair. Genetics. 2004;166:1673-86 pubmed
    The MPH1 gene from Saccharomyces cerevisiae, encoding a member of the DEAH family of proteins, had been identified by virtue of the spontaneous mutator phenotype of respective deletion mutants...
  5. Ward T, Dudasova Z, Sarkar S, Bhide M, Vlasáková D, Chovanec M, et al. Components of a Fanconi-like pathway control Pso2-independent DNA interstrand crosslink repair in yeast. PLoS Genet. 2012;8:e1002884 pubmed publisher
    ..The FA repair pathway is not thought to be conserved in budding yeast, and although the yeast Mph1 helicase is a putative homolog of human FANCM, yeast cells disrupted for MPH1 are not sensitive to ICLs...
  6. Lyndaker A, Goldfarb T, Alani E. Mutants defective in Rad1-Rad10-Slx4 exhibit a unique pattern of viability during mating-type switching in Saccharomyces cerevisiae. Genetics. 2008;179:1807-21 pubmed publisher
  7. Chen Y, Choi K, Szakal B, Arenz J, Duan X, Ye H, et al. Interplay between the Smc5/6 complex and the Mph1 helicase in recombinational repair. Proc Natl Acad Sci U S A. 2009;106:21252-7 pubmed publisher
    ..Here we report that the budding yeast Smc5/6 complex directly binds to the DNA helicase Mph1. Mph1 and its helicase activity define a replication-associated recombination subpathway...
  8. Daee D, Ferrari E, Longerich S, Zheng X, Xue X, Branzei D, et al. Rad5-dependent DNA repair functions of the Saccharomyces cerevisiae FANCM protein homolog Mph1. J Biol Chem. 2012;287:26563-75 pubmed publisher
    ..In this study we genetically characterized a conserved yeast ICL repair pathway composed of the yeast homologs (Mph1, Chl1, Mhf1, Mhf2) of four FA proteins (FANCM, FANCJ, MHF1, MHF2)...
  9. Chavez A, Agrawal V, Johnson F. Homologous recombination-dependent rescue of deficiency in the structural maintenance of chromosomes (Smc) 5/6 complex. J Biol Chem. 2011;286:5119-25 pubmed publisher
    ..Here we investigate how deletion of MPH1, encoding the orthologue of the human FANCM DNA helicase, rescues the DNA damage sensitivity of smc5/6 but not sgs1?..

More Information

Publications44

  1. Banerjee S, Smith S, Oum J, Liaw H, Hwang J, Sikdar N, et al. Mph1p promotes gross chromosomal rearrangement through partial inhibition of homologous recombination. J Cell Biol. 2008;181:1083-93 pubmed publisher
    ..In yeast, multiple pathways cooperate to suppress GCR. In a screen for genes that promote GCR, we identified MPH1, which encodes a 3'-5' DNA helicase...
  2. Tay Y, Sidebotham J, Wu L. Mph1 requires mismatch repair-independent and -dependent functions of MutSalpha to regulate crossover formation during homologous recombination repair. Nucleic Acids Res. 2010;38:1889-901 pubmed publisher
    In budding yeast the DNA helicase Mph1 prevents genome rearrangements during ectopic homologous recombination (HR) by suppressing the formation of crossovers (COs)...
  3. Ede C, Rudolph C, Lehmann S, Schürer K, Kramer W. Budding yeast Mph1 promotes sister chromatid interactions by a mechanism involving strand invasion. DNA Repair (Amst). 2011;10:45-55 pubmed publisher
    ..In cells lacking Mph1, a member of the FANCM family of DNA helicases, that has been implicated in a pathway for fork reactivation ..
  4. Kang Y, Kang M, Kim J, Lee C, Cho I, Hurwitz J, et al. The MPH1 gene of Saccharomyces cerevisiae functions in Okazaki fragment processing. J Biol Chem. 2009;284:10376-86 pubmed publisher
    Saccharomyces cerevisiae MPH1 was first identified as a gene encoding a 3' to 5' DNA helicase, which when deleted leads to a mutator phenotype...
  5. Prakash R, Satory D, Dray E, Papusha A, Scheller J, Kramer W, et al. Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination. Genes Dev. 2009;23:67-79 pubmed publisher
    ..We show here that budding yeast Mph1, an ortholog of human FancM helicase, utilizes its helicase activity to suppress spontaneous unequal sister ..
  6. Mitchel K, Lehner K, Jinks Robertson S. Heteroduplex DNA position defines the roles of the Sgs1, Srs2, and Mph1 helicases in promoting distinct recombination outcomes. PLoS Genet. 2013;9:e1003340 pubmed publisher
    The contributions of the Sgs1, Mph1, and Srs2 DNA helicases during mitotic double-strand break (DSB) repair in yeast were investigated using a gap-repair assay...
  7. Mazon G, Symington L. Mph1 and Mus81-Mms4 prevent aberrant processing of mitotic recombination intermediates. Mol Cell. 2013;52:63-74 pubmed publisher
    ..Here we show that the Saccharomyces cerevisiae Mph1 helicase prevents crossovers between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10 cleavage...
  8. Meetei A, Medhurst A, Ling C, Xue Y, Singh T, Bier P, et al. A human ortholog of archaeal DNA repair protein Hef is defective in Fanconi anemia complementation group M. Nat Genet. 2005;37:958-63 pubmed
    ..FAAP250 or FANCM has sequence similarity to known DNA-repair proteins, including archaeal Hef, yeast MPH1 and human ERCC4 or XPF...
  9. Luke Glaser S, Luke B. The Mph1 helicase can promote telomere uncapping and premature senescence in budding yeast. PLoS ONE. 2012;7:e42028 pubmed publisher
    ..Here we have investigated how the yeast helicase, Mph1, affects DNA repair at both DSBs and telomeres...
  10. Choi K, Szakal B, Chen Y, Branzei D, Zhao X. The Smc5/6 complex and Esc2 influence multiple replication-associated recombination processes in Saccharomyces cerevisiae. Mol Biol Cell. 2010;21:2306-14 pubmed publisher
    ..Here, we show that three of these factors, Mph1, Mms2, and the Shu complex, can act independently to promote the formation of recombination intermediates during ..
  11. Zheng X, Prakash R, Saro D, Longerich S, Niu H, Sung P. Processing of DNA structures via DNA unwinding and branch migration by the S. cerevisiae Mph1 protein. DNA Repair (Amst). 2011;10:1034-43 pubmed publisher
    The budding yeast Mph1 protein, the putative ortholog of human FANCM, possesses a 3' to 5' DNA helicase activity and is capable of disrupting the D-loop structure to suppress chromosome arm crossovers in mitotic homologous recombination...
  12. Schmidt K, Viebranz E, Harris L, Mirzaei Souderjani H, Syed S, Medicus R. Defects in DNA lesion bypass lead to spontaneous chromosomal rearrangements and increased cell death. Eukaryot Cell. 2010;9:315-24 pubmed publisher
    Rev3 polymerase and Mph1 DNA helicase participate in error-prone and error-free pathways, respectively, for the bypassing of template lesions during DNA replication...
  13. Prakash R, Krejci L, Van Komen S, Anke Schürer K, Kramer W, Sung P. Saccharomyces cerevisiae MPH1 gene, required for homologous recombination-mediated mutation avoidance, encodes a 3' to 5' DNA helicase. J Biol Chem. 2005;280:7854-60 pubmed
    The MPH1 (mutator pHenotype 1) gene of Saccharomyces cerevisiae was identified on the basis of elevated spontaneous mutation rates of haploid cells deleted for this gene...
  14. Lafuente Barquero J, Luke Glaser S, Graf M, Silva S, Gómez González B, Lockhart A, et al. The Smc5/6 complex regulates the yeast Mph1 helicase at RNA-DNA hybrid-mediated DNA damage. PLoS Genet. 2017;13:e1007136 pubmed publisher
    ..We demonstrate that Mph1, the yeast homolog of Fanconi anemia protein M (FANCM), is required for cell viability in the absence of RNase H ..
  15. Dummer A, Su Z, Cherney R, Choi K, DENU J, Zhao X, et al. Binding of the Fkh1 Forkhead Associated Domain to a Phosphopeptide within the Mph1 DNA Helicase Regulates Mating-Type Switching in Budding Yeast. PLoS Genet. 2016;12:e1006094 pubmed publisher
    ..In contrast, Fkh1's interaction with the Mph1 DNA repair helicase regulated donor preference during mating-type switching...
  16. Lehner K, Jinks Robertson S. Shared genetic pathways contribute to the tolerance of endogenous and low-dose exogenous DNA damage in yeast. Genetics. 2014;198:519-30 pubmed publisher
    ..These results have important implications when considering what constitutes a safe and acceptable level of exogenous DNA damage. ..
  17. Hoopes J, Hughes A, Hobson L, Cortez L, Brown A, Roberts S. Avoidance of APOBEC3B-induced mutation by error-free lesion bypass. Nucleic Acids Res. 2017;45:5243-5254 pubmed publisher
    ..However, strains without error-free lesion bypass proteins Ubc13, Mms2 and Mph1 displayed respective 4.9-, 2.8- and 7.8-fold higher frequency of APOBEC3B-induced CanR...
  18. Allen Soltero S, Martinez S, Putnam C, Kolodner R. A saccharomyces cerevisiae RNase H2 interaction network functions to suppress genome instability. Mol Cell Biol. 2014;34:1521-34 pubmed publisher
    ..This analysis suggests that cells with RNase H2 defects have increased levels of DNA damage and depend on other pathways of DNA metabolism to overcome the deleterious effects of this DNA damage. ..
  19. Xue X, Choi K, Bonner J, Chiba T, Kwon Y, Xu Y, et al. Restriction of replication fork regression activities by a conserved SMC complex. Mol Cell. 2014;56:436-45 pubmed publisher
    ..the structural maintenance of chromosome complex Smc5-Smc6 restrains the replication fork regression activity of Mph1 helicase, but not its D loop disruptive activity...
  20. Xue X, Papusha A, Choi K, Bonner J, Kumar S, Niu H, et al. Differential regulation of the anti-crossover and replication fork regression activities of Mph1 by Mte1. Genes Dev. 2016;30:687-99 pubmed publisher
    We identified Mte1 (Mph1-associated telomere maintenance protein 1) as a multifunctional regulator of Saccharomyces cerevisiae Mph1, a member of the FANCM family of DNA motor proteins important for DNA replication fork repair and ..
  21. Chen Y, Szakal B, Castellucci F, Branzei D, Zhao X. DNA damage checkpoint and recombinational repair differentially affect the replication stress tolerance of Smc6 mutants. Mol Biol Cell. 2013;24:2431-41 pubmed publisher
    ..Research in budding yeast shows that removal of the DNA helicase Mph1 improves survival of cells with defective Smc5/6 complex under replication stress...
  22. Vasianovich Y, Harrington L, Makovets S. Break-induced replication requires DNA damage-induced phosphorylation of Pif1 and leads to telomere lengthening. PLoS Genet. 2014;10:e1004679 pubmed publisher
    ..We propose a model whereby the passage of BIR forks through telomeres promotes telomerase activity and leads to telomere lengthening. ..
  23. Xue X, Choi K, Bonner J, Szakal B, Chen Y, Papusha A, et al. Selective modulation of the functions of a conserved DNA motor by a histone fold complex. Genes Dev. 2015;29:1000-5 pubmed publisher
    Budding yeast Mph1 helicase and its orthologs drive multiple DNA transactions. Elucidating the mechanisms that regulate these motor proteins is central to understanding genome maintenance processes...
  24. Kramarz K, Litwin I, Cal Bakowska M, Szakal B, Branzei D, Wysocki R, et al. Swi2/Snf2-like protein Uls1 functions in the Sgs1-dependent pathway of maintenance of rDNA stability and alleviation of replication stress. DNA Repair (Amst). 2014;21:24-35 pubmed publisher
    ..of the replication stress response pathways mediated by the endonucleases Mus81 and Yen1 and the helicases Mph1 and Srs2...
  25. Choi K, Batke S, Szakal B, Lowther J, Hao F, Sarangi P, et al. Concerted and differential actions of two enzymatic domains underlie Rad5 contributions to DNA damage tolerance. Nucleic Acids Res. 2015;43:2666-77 pubmed publisher
    ..These findings delineate how two Rad5 enzymatic domains concertedly influence PCNA modification, and unveil their discrete contributions to stress tolerance. ..
  26. Putnam C, Hayes T, Kolodner R. Post-replication repair suppresses duplication-mediated genome instability. PLoS Genet. 2010;6:e1000933 pubmed publisher
    ..Our analysis is consistent with models in which PRR prevents replication damage from becoming double strand breaks (DSBs) and/or regulates the activity of HR on DSBs. ..
  27. Silva S, Altmannova V, Luke Glaser S, Henriksen P, Gallina I, Yang X, et al. Mte1 interacts with Mph1 and promotes crossover recombination and telomere maintenance. Genes Dev. 2016;30:700-17 pubmed publisher
    b>Mph1 is a member of the conserved FANCM family of DNA motor proteins that play key roles in genome maintenance processes underlying Fanconi anemia, a cancer predisposition syndrome in humans...
  28. Kucejova B, Foury F. Search for protein partners of mitochondrial single-stranded DNA-binding protein Rim1p using a yeast two-hybrid system. Folia Microbiol (Praha). 2003;48:183-8 pubmed
  29. Mehta A, Beach A, Haber J. Homology Requirements and Competition between Gene Conversion and Break-Induced Replication during Double-Strand Break Repair. Mol Cell. 2017;65:515-526.e3 pubmed publisher
    ..With short second-end homology, absence of the RecQ helicase Sgs1 promotes gene conversion, whereas deletion of the FANCM-related Mph1 helicase promotes BIR.
  30. Ang J, Duffy S, Segovia R, Stirling P, Hieter P. Dosage Mutator Genes in Saccharomyces cerevisiae: A Novel Mutator Mode-of-Action of the Mph1 DNA Helicase. Genetics. 2016;204:975-986 pubmed publisher
    ..screened ?5100 plasmids, each overexpressing a unique single gene, and characterized the five strongest mutators, MPH1 (mutator phenotype 1), RRM3, UBP12, PIF1, and DNA2 We show that, for MPH1, the yeast homolog of Fanconi Anemia ..
  31. Stafa A, Donnianni R, Timashev L, Lam A, Symington L. Template switching during break-induced replication is promoted by the Mph1 helicase in Saccharomyces cerevisiae. Genetics. 2014;196:1017-28 pubmed publisher
    ..The frequency of BIR increased in the mph1? mutant, but the percentage of template switch events was significantly decreased, revealing an important role for ..
  32. Kang Y, Munashingha P, Lee C, Nguyen T, Seo Y. Biochemical studies of the Saccharomyces cerevisiae Mph1 helicase on junction-containing DNA structures. Nucleic Acids Res. 2012;40:2089-106 pubmed publisher
    Saccharomyces cerevisiae Mph1 is a 3-5' DNA helicase, required for the maintenance of genome integrity...
  33. Singh S, Shemesh K, Liefshitz B, Kupiec M. Genetic and physical interactions between the yeast ELG1 gene and orthologs of the Fanconi anemia pathway. Cell Cycle. 2013;12:1625-36 pubmed publisher
    ..We show that Elg1 physically contacts the Mhf1/Mhf2 histone-like complex and genetically interacts with MPH1 (ortholog of the FANCM helicase) and CHL1 (ortholog of the FANCJ helicase) genes...
  34. Jain S, Sugawara N, Mehta A, Ryu T, Haber J. Sgs1 and Mph1 Helicases Enforce the Recombination Execution Checkpoint During DNA Double-Strand Break Repair in Saccharomyces cerevisiae. Genetics. 2016;203:667-75 pubmed publisher
    ..Here we report that the simultaneous deletion of two 3'?5' helicases, Sgs1 and Mph1, largely abolishes the REC-mediated lag normally observed during the repair of large gaps and BIR substrates, which ..
  35. Kim D, Harris B, Wang F, Seidel C, McCroskey S, Gerton J. Mms21 SUMO Ligase Activity Promotes Nucleolar Function in Saccharomyces cerevisiae. Genetics. 2016;204:645-658 pubmed
    ..Deletion of a previously identified strong suppressor, MPH1, rescues both the accumulation of ribosome subunits and translational stress...