Gene Symbol: MNS1
Description: mannosyl-oligosaccharide 1,2-alpha-mannosidase
Alias: mannosyl-oligosaccharide 1,2-alpha-mannosidase
Species: Saccharomyces cerevisiae S288c
Puccia R, Grondin B, Herscovics A. Disruption of the processing alpha-mannosidase gene does not prevent outer chain synthesis in Saccharomyces cerevisiae. Biochem J. 1993;290 ( Pt 1):21-6 pubmed
..cerevisiae disrupted in the MNS1 gene encoding a specific alpha 1,2-mannosidase responsible for Man8GlcNAc2 synthesis [Camirand, Heysen, Grondin and ..
Knop M, Hauser N, Wolf D. N-Glycosylation affects endoplasmic reticulum degradation of a mutated derivative of carboxypeptidase yscY in yeast. Yeast. 1996;12:1229-38 pubmed
..Our results suggest that the ER of yeast contains a glycosylation-dependent quality control system, as has been shown for higher eukaryotic cells. ..
Camirand A, Heysen A, Grondin B, Herscovics A. Glycoprotein biosynthesis in Saccharomyces cerevisiae. Isolation and characterization of the gene encoding a specific processing alpha-mannosidase. J Biol Chem. 1991;266:15120-7 pubmed
..The gene, designated MNS1, encodes a 549-amino acid polypeptide of calculated molecular size 63,017 Da produced by an mRNA species of ..
Burke J, Lipari F, Igdoura S, Herscovics A. The Saccharomyces cerevisiae processing alpha 1,2-mannosidase is localized in the endoplasmic reticulum, independently of known retrieval motifs. Eur J Cell Biol. 1996;70:298-305 pubmed
..As Mns1p contains none of the known endoplasmic reticulum retrieval motifs (HDEL, KK or RR), these results suggest that Mns1p is localized in the endoplasmic reticulum by a different retentin mechanism. ..
Lipari F, Herscovics A. Role of the cysteine residues in the alpha1,2-mannosidase involved in N-glycan biosynthesis in Saccharomyces cerevisiae. The conserved Cys340 and Cys385 residues form an essential disulfide bond. J Biol Chem. 1996;271:27615-22 pubmed
..Since Cys340 and Cys385 are conserved residues, it is likely that this disulfide bond is important to maintain the correct structure in the other members of the alpha1, 2-mannosidase family. ..
Jakob C, Burda P, Roth J, Aebi M. Degradation of misfolded endoplasmic reticulum glycoproteins in Saccharomyces cerevisiae is determined by a specific oligosaccharide structure. J Cell Biol. 1998;142:1223-33 pubmed
..They may indicate the presence of a Man8GlcNAc2-binding lectin involved in targeting of misfolded glycoproteins to degradation in S. cerevisiae. ..
Massaad M, Herscovics A. Interaction of the endoplasmic reticulum alpha 1,2-mannosidase Mns1p with Rer1p using the split-ubiquitin system. J Cell Sci. 2001;114:4629-35 pubmed
..Furthermore, the split-ubiquitin system demonstrates that the C-terminal of Rer1p is in the cytosol. ..
Herscovics A, Romero P, Tremblay L. The specificity of the yeast and human class I ER alpha 1,2-mannosidases involved in ER quality control is not as strict previously reported. Glycobiology. 2002;12:14G-15G pubmed
Hosomi A, Tanabe K, Hirayama H, Kim I, Rao H, Suzuki T. Identification of an Htm1 (EDEM)-dependent, Mns1-independent Endoplasmic Reticulum-associated Degradation (ERAD) pathway in Saccharomyces cerevisiae: application of a novel assay for glycoprotein ERAD. J Biol Chem. 2010;285:24324-34 pubmed publisher
..Interestingly, ER-mannosidase Mns1 was not involved in RTA degradation, but it was dependent on Htm1 (ERAD-related alpha-mannosidase in yeast) and ..