Gene Symbol: MKC7
Description: aspartyl protease
Alias: YPS2, aspartyl protease
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Krysan D, Ting E, Abeijon C, Kroos L, Fuller R. Yapsins are a family of aspartyl proteases required for cell wall integrity in Saccharomyces cerevisiae. Eukaryot Cell. 2005;4:1364-74 pubmed
    ..Taken together, these data indicate that the yapsins play an important role in glucan homeostasis in S. cerevisiae and that yapsin homologues may play a similar role in the pathogenic yeasts C. albicans and C. glabrata. ..
  2. Hamada K, Terashima H, Arisawa M, Yabuki N, Kitada K. Amino acid residues in the omega-minus region participate in cellular localization of yeast glycosylphosphatidylinositol-attached proteins. J Bacteriol. 1999;181:3886-9 pubmed
    ..These correlations clearly suggest that GPI-attached proteins have two different signals which act positively or negatively in cell wall incorporation for their cellular localization. ..
  3. Komano H, Rockwell N, Wang G, Krafft G, Fuller R. Purification and characterization of the yeast glycosylphosphatidylinositol-anchored, monobasic-specific aspartyl protease yapsin 2 (Mkc7p). J Biol Chem. 1999;274:24431-7 pubmed
    The Saccharomyces cerevisiae YPS2 (formerly MKC7) gene product is a glycosylphosphatidylinositol-linked aspartyl protease that functions as a yeast secretase...
  4. Miller K, Didone L, Krysan D. Extracellular secretion of overexpressed glycosylphosphatidylinositol-linked cell wall protein Utr2/Crh2p as a novel protein quality control mechanism in Saccharomyces cerevisiae. Eukaryot Cell. 2010;9:1669-79 pubmed publisher
    ..cerevisiae. This model provides an explanation for the deleterious effects of Yps1/2p on the industrial production of some recombinant proteins in S. cerevisiae. ..
  5. Komano H, Fuller R. Shared functions in vivo of a glycosyl-phosphatidylinositol-linked aspartyl protease, Mkc7, and the proprotein processing protease Kex2 in yeast. Proc Natl Acad Sci U S A. 1995;92:10752-6 pubmed
    ..MKC7 encodes an aspartyl protease most closely related to product of the YAP3 gene, a previously isolated multicopy suppressor of the pro-..
  6. Oluwatosin Y, Kane P. Mutations in the yeast KEX2 gene cause a Vma(-)-like phenotype: a possible role for the Kex2 endoprotease in vacuolar acidification. Mol Cell Biol. 1998;18:1534-43 pubmed
    ..This is the first time a mutation of this type has been identified. ..
  7. Vadaie N, Dionne H, Akajagbor D, Nickerson S, Krysan D, Cullen P. Cleavage of the signaling mucin Msb2 by the aspartyl protease Yps1 is required for MAPK activation in yeast. J Cell Biol. 2008;181:1073-81 pubmed publisher
    ..Cleavage of the extracellular inhibitory domain of Msb2p by the aspartyl protease Yps1p generated the active form of the protein by a mechanism incorporating cellular nutritional status...
  8. Friedmann E, Salzberg Y, Weinberger A, Shaltiel S, Gerst J. YOS9, the putative yeast homolog of a gene amplified in osteosarcomas, is involved in the endoplasmic reticulum (ER)-Golgi transport of GPI-anchored proteins. J Biol Chem. 2002;277:35274-81 pubmed
    ..Yos9 binds directly to Gas1 and Mkc7 and accelerates Gas1 transport and processing in cells overexpressing YOS9...
  9. Zhang W, Espinoza D, Hines V, Innis M, Mehta P, Miller D. Characterization of beta-amyloid peptide precursor processing by the yeast Yap3 and Mkc7 proteases. Biochim Biophys Acta. 1997;1359:110-22 pubmed
    ..Deletions of the genes encoding the GPI-linked aspartyl proteases Yap3 and Mkc7 decreased alpha-secretase activity by 56 and 29%, respectively; whereas, the double deletion decreased the activity ..

More Information


  1. Tomishige N, Noda Y, Adachi H, Shimoi H, Takatsuki A, Yoda K. Mutations that are synthetically lethal with a gas1Delta allele cause defects in the cell wall of Saccharomyces cerevisiae. Mol Genet Genomics. 2003;269:562-73 pubmed
    ..A cell wall defect was also found in the kex2Delta mutant, which was suppressible by multiple copies of the MKC7 or YAP3 gene, both of which encode other GPI-anchored proteases...
  2. Cho E, Cheon S, Kim H, Choo J, Lee D, Ryu H, et al. Multiple-yapsin-deficient mutant strains for high-level production of intact recombinant proteins in Saccharomyces cerevisiae. J Biotechnol. 2010;149:1-7 pubmed publisher
    ..The present data strongly indicate the involvement of other yapsin members besides Yps1p in the proteolysis of secretory recombinant proteins, particularly under high-density growth conditions. ..