Gene Symbol: MIH1
Description: putative tyrosine protein phosphatase MIH1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Lin F, Arndt K. The role of Saccharomyces cerevisiae type 2A phosphatase in the actin cytoskeleton and in entry into mitosis. EMBO J. 1995;14:2745-59 pubmed
    ..Unlike wild-type strains, a PP2A-deficient strain was sensitive to the loss of MIH1, which is a homolog of the Schizosaccharomyces pombe mitotic inducer cdc25+...
  2. Booher R, Deshaies R, Kirschner M. Properties of Saccharomyces cerevisiae wee1 and its differential regulation of p34CDC28 in response to G1 and G2 cyclins. EMBO J. 1993;12:3417-26 pubmed
    ..short spindles whereas deletion of SWE1 did not alter the cell cycle but did eliminate the G2 delay observed in mih1- mutants...
  3. Longtine M, Theesfeld C, McMillan J, Weaver E, Pringle J, Lew D. Septin-dependent assembly of a cell cycle-regulatory module in Saccharomyces cerevisiae. Mol Cell Biol. 2000;20:4049-61 pubmed
    ..e., after a bud has formed). ..
  4. Pal G, Paraz M, Kellogg D. Regulation of Mih1/Cdc25 by protein phosphatase 2A and casein kinase 1. J Cell Biol. 2008;180:931-45 pubmed publisher
    ..more about the mechanisms that regulate entry into mitosis, we have characterized the function and regulation of Mih1, the budding yeast homologue of Cdc25...
  5. Russell P, Moreno S, Reed S. Conservation of mitotic controls in fission and budding yeasts. Cell. 1989;57:295-303 pubmed
    ..cerevisiae. The wee1+ activity is counteracted in S. cerevisiae by the gene product of MIH1, a newly identified gene capable of encoding a protein of MW 54,000, which is a structural and functional homolog ..
  6. McMillan J, Theesfeld C, Harrison J, Bardes E, Lew D. Determinants of Swe1p degradation in Saccharomyces cerevisiae. Mol Biol Cell. 2002;13:3560-75 pubmed
    ..The other region did not appear to affect interactions with known Swe1p regulators, suggesting that other as-yet-unknown regulators exist. ..
  7. Yasutis K, Vignali M, Ryder M, Tameire F, Dighe S, Fields S, et al. Zds2p regulates Swe1p-dependent polarized cell growth in Saccharomyces cerevisiae via a novel Cdc55p interaction domain. Mol Biol Cell. 2010;21:4373-86 pubmed publisher
    ..This negative regulation requires the CDC55 gene. Together these data indicate that the Cdc55p/Zds2p module has a function in the regulation of the Swe1p-dependent G2/M checkpoint. ..
  8. Harrison J, Bardes E, Ohya Y, Lew D. A role for the Pkc1p/Mpk1p kinase cascade in the morphogenesis checkpoint. Nat Cell Biol. 2001;3:417-20 pubmed
    ..The G2 delay involves stabilization of Swe1p in response to various actin perturbations, although this alone is insufficient to produce a long G2 delay. ..
  9. Yano K, Uesono Y, Yoshida S, Kikuchi A, Kashiwazaki J, Mabuchi I, et al. Mih1/Cdc25 is negatively regulated by Pkc1 in Saccharomyces cerevisiae. Genes Cells. 2013;18:425-41 pubmed publisher
    ..In Saccharomyces cerevisiae, regulation of Mih1 (Cdc25 homologue) remains unclear because inactivation/degradation of Swe1 (Wee1 homologue) is the main trigger for ..

More Information


  1. Szkotnicki L, Crutchley J, Zyla T, Bardes E, Lew D. The checkpoint kinase Hsl1p is activated by Elm1p-dependent phosphorylation. Mol Biol Cell. 2008;19:4675-86 pubmed publisher
    ..We identified elm1 mutants in a screen for defects in Swe1p degradation and show that a phosphomimic T273E mutation in HSL1 bypasses the need for Elm1p in this pathway. ..
  2. Crutchley J, King K, Keaton M, Szkotnicki L, Orlando D, Zyla T, et al. Molecular dissection of the checkpoint kinase Hsl1p. Mol Biol Cell. 2009;20:1926-36 pubmed publisher
    ..We suggest that Hsl1p responds to alterations in septin organization, which themselves occur in response to the local geometry of the cell cortex. ..
  3. Kim J, Rose M. Stable Pseudohyphal Growth in Budding Yeast Induced by Synergism between Septin Defects and Altered MAP-kinase Signaling. PLoS Genet. 2015;11:e1005684 pubmed publisher
    ..Taken together, our findings show that budding yeast can access a stable constitutive pseudohyphal growth state with very few genetic and regulatory changes. ..
  4. Rossio V, Kazatskaya A, Hirabayashi M, Yoshida S. Comparative genetic analysis of PP2A-Cdc55 regulators in budding yeast. Cell Cycle. 2014;13:2073-83 pubmed publisher
  5. Juanes M, Khoueiry R, Kupka T, Castro A, Mudrak I, Ogris E, et al. Budding yeast greatwall and endosulfines control activity and spatial regulation of PP2A(Cdc55) for timely mitotic progression. PLoS Genet. 2013;9:e1003575 pubmed publisher
    ..However, this conserved module is adapted to account for differences in the regulation of mitotic entry in different organisms. ..
  6. Matsuda K, Makise M, Sueyasu Y, Takehara M, Asano T, Mizushima T. Yeast two-hybrid analysis of the origin recognition complex of Saccharomyces cerevisiae: interaction between subunits and identification of binding proteins. FEMS Yeast Res. 2007;7:1263-9 pubmed
    ..We discuss roles of these interactions in functions of ORC. ..
  7. McQueen J, van Dyk D, Young B, Loewen C, Measday V. The Mck1 GSK-3 kinase inhibits the activity of Clb2-Cdk1 post-nuclear division. Cell Cycle. 2012;11:3421-32 pubmed publisher
    ..to increased Clb2-Cdk1 activity caused either by overexpression of Clb2 or the Cdk1-activating phosphatase Mih1. Deletion of the Cdk1 inhibitory kinase, SWE1, in combination with a mck1? mutant results in a synthetic growth ..
  8. Starovoytova A, Sorokin M, Sokolov S, Severin F, Knorre D. Mitochondrial signaling in Saccharomyces cerevisiae pseudohyphae formation induced by butanol. FEMS Yeast Res. 2013;13:367-74 pubmed publisher
    ..Interestingly, repression of the MIH1 gene encoding phosphatase activator of the G2/M transition partially restores the ability of yeast to form ..
  9. Cui T, Peterson T, Burd C. A CDC25 family protein phosphatase gates cargo recognition by the Vps26 retromer subunit. elife. 2017;6: pubmed publisher
    ..spontaneously arising mutation that activates the yeast (Saccharomyces cerevisiae) CDC25 family phosphatase, Mih1, results in accelerated turnover of a subset of endocytosed plasma membrane proteins due to deficient sorting into ..
  10. Raspelli E, Cassani C, Lucchini G, Fraschini R. Budding yeast Dma1 and Dma2 participate in regulation of Swe1 levels and localization. Mol Biol Cell. 2011;22:2185-97 pubmed publisher
    ..Taken together, the data highlight a previously unknown role of these proteins in the complex regulation of Swe1 and suggest that they might contribute to control, directly or indirectly, Swe1 ubiquitylation. ..
  11. Snead J, Sullivan M, Lowery D, Cohen M, Zhang C, Randle D, et al. A coupled chemical-genetic and bioinformatic approach to Polo-like kinase pathway exploration. Chem Biol. 2007;14:1261-72 pubmed
    ..Finally, an examination of Cdc5 binding by SPB-localized proteins expanded our knowledge of Cdc5 function at the SPB. ..
  12. Boronat S, Campbell J. Mitotic Cdc6 stabilizes anaphase-promoting complex substrates by a partially Cdc28-independent mechanism, and this stabilization is suppressed by deletion of Cdc55. Mol Cell Biol. 2007;27:1158-71 pubmed
    ..This demonstrates that the levels and timing of accumulation of Cdc6p in mitosis are appropriate for mediating the modulation of APC/Cdc20. ..
  13. Martinez Anaya C, Dickinson J, Sudbery P. In yeast, the pseudohyphal phenotype induced by isoamyl alcohol results from the operation of the morphogenesis checkpoint. J Cell Sci. 2003;116:3423-31 pubmed
    ..These observations show that IAA induces the Swe1-dependent morphogenesis checkpoint and so the resulting pseudohyphal phenotype arises in an entirely different way from the formation of pseudohyphae induced by nitrogen-limited growth. ..
  14. Kennedy E, Dysart M, Lianga N, Williams E, Pilon S, Doré C, et al. Redundant Regulation of Cdk1 Tyrosine Dephosphorylation in Saccharomyces cerevisiae. Genetics. 2016;202:903-10 pubmed publisher
    ..In contrast, deletion of MIH1, the cdc25 homolog in Saccharomyces cerevisiae, is viable...
  15. Finnigan G, Sterling S, Duvalyan A, Liao E, Sargsyan A, Garcia G, et al. Coordinate action of distinct sequence elements localizes checkpoint kinase Hsl1 to the septin collar at the bud neck in Saccharomyces cerevisiae. Mol Biol Cell. 2016;27:2213-33 pubmed publisher
  16. Vinton P, Weinert T. A Slowed Cell Cycle Stabilizes the Budding Yeast Genome. Genetics. 2017;206:811-828 pubmed publisher
    ..And, some chromosome errors may not signal checkpoint-mediated responses, or do not sufficiently signal to allow correction, and their correction benefits from this "time checkpoint." ..