Gene Symbol: MID1
Description: Mid1p
Alias: Mid1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Courchesne W, Vlasek C, Klukovich R, Coffee S. Ethanol induces calcium influx via the Cch1-Mid1 transporter in Saccharomyces cerevisiae. Arch Microbiol. 2011;193:323-34 pubmed publisher
    ..The Ca(2+) flux is primarily via the Cch1 Ca(2+) influx channel because strains carrying deletions of the cch1 and mid1 genes show greater than 90% reduction in Ca(2+) flux...
  2. Yoshimura H, Tada T, Iida H. Subcellular localization and oligomeric structure of the yeast putative stretch-activated Ca2+ channel component Mid1. Exp Cell Res. 2004;293:185-95 pubmed
    The yeast Mid1 protein with an apparent molecular mass of 100 kDa is required for Ca2+ influx stimulated by the mating pheromone and by a capacitative calcium entrylike mechanism acting in response to Ca2+ depletion from the endoplasmic ..
  3. Kanzaki M, Nagasawa M, Kojima I, Sato C, Naruse K, Sokabe M, et al. Molecular identification of a eukaryotic, stretch-activated nonselective cation channel. Science. 1999;285:882-6 pubmed
    ..However, genes encoding such channels have not been identified in eukaryotes. The yeast MID1 gene product (Mid1) is required for calcium influx in the yeast Saccharomyces cerevisiae...
  4. Peiter E, Fischer M, Sidaway K, Roberts S, Sanders D. The Saccharomyces cerevisiae Ca2+ channel Cch1pMid1p is essential for tolerance to cold stress and iron toxicity. FEBS Lett. 2005;579:5697-703 pubmed
    Cch1p and Mid1p are components of a high-affinity Ca(2+)-permeable channel in the yeast plasma membrane...
  5. Martin D, Kim H, Mackin N, Maldonado Baez L, Evangelista C, BEAUDRY V, et al. New regulators of a high affinity Ca2+ influx system revealed through a genome-wide screen in yeast. J Biol Chem. 2011;286:10744-54 pubmed publisher
    ..HACS consists of two known subunits, Cch1 and Mid1, that are homologous and analogous to the catalytic ?-subunits and regulatory ?2?-subunits of mammalian voltage-..
  6. D Hooge P, Coun C, Van Eyck V, Faes L, Ghillebert R, Mariën L, et al. Ca(2+) homeostasis in the budding yeast Saccharomyces cerevisiae: Impact of ER/Golgi Ca(2+) storage. Cell Calcium. 2015;58:226-35 pubmed publisher
    ..In the pmr1Δ strain, these effects were insensitive to calcineurin activity, independent of Cch1/Mid1 Ca(2+) channels and Pmc1 but required Vcx1...
  7. Kajiwara K, Muneoka T, Watanabe Y, Karashima T, Kitagaki H, Funato K. Perturbation of sphingolipid metabolism induces endoplasmic reticulum stress-mediated mitochondrial apoptosis in budding yeast. Mol Microbiol. 2012;86:1246-61 pubmed publisher
  8. Vlahakis A, Lopez Muniozguren N, Powers T. Calcium channel regulator Mid1 links TORC2-mediated changes in mitochondrial respiration to autophagy. J Cell Biol. 2016;215:779-788 pubmed
    ..Here we demonstrate that activation of calcineurin requires Mid1, an endoplasmic reticulum-localized calcium channel regulatory protein implicated in the oxidative stress response...
  9. Paidhungat M, Garrett S. A homolog of mammalian, voltage-gated calcium channels mediates yeast pheromone-stimulated Ca2+ uptake and exacerbates the cdc1(Ts) growth defect. Mol Cell Biol. 1997;17:6339-47 pubmed
    ..One of the cdc1(Ts) suppressors is identical to a gene, MID1, recently implicated in mating pheromone-stimulated Ca2+ uptake...

More Information


  1. Iida K, Tada T, Iida H. Molecular cloning in yeast by in vivo homologous recombination of the yeast putative alpha1 subunit of the voltage-gated calcium channel. FEBS Lett. 2004;576:291-6 pubmed
    ..coli. Overexpression of the cloned CCH1 or MID1 alone, which encodes a putative stretch-activated Ca2+ channel component, does not increase Ca2+ uptake activity, ..
  2. Groppi S, Belotti F, Brandão R, Martegani E, Tisi R. Glucose-induced calcium influx in budding yeast involves a novel calcium transport system and can activate calcineurin. Cell Calcium. 2011;49:376-86 pubmed publisher
  3. Locke E, Bonilla M, Liang L, Takita Y, Cunningham K. A homolog of voltage-gated Ca(2+) channels stimulated by depletion of secretory Ca(2+) in yeast. Mol Cell Biol. 2000;20:6686-94 pubmed
    ..Cch1p and Mid1p were localized to the plasma membrane, coimmunoprecipitated from solubilized membranes, and shown to function ..
  4. Noma S, Iida K, Iida H. Polarized morphogenesis regulator Spa2 is required for the function of putative stretch-activated Ca2+-permeable channel component Mid1 in Saccharomyces cerevisiae. Eukaryot Cell. 2005;4:1353-63 pubmed
    b>Mid1 is a putative stretch-activated Ca2+ channel component and is required for the maintenance of viability in the mating process...
  5. Rigamonti M, Groppi S, Belotti F, Ambrosini R, Filippi G, Martegani E, et al. Hypotonic stress-induced calcium signaling in Saccharomyces cerevisiae involves TRP-like transporters on the endoplasmic reticulum membrane. Cell Calcium. 2015;57:57-68 pubmed publisher
    ..compartments is peculiarly inhibited by external calcium in a calcineurin-independent and Cch1-, but not Mid1-, driven manner...
  6. Courchesne W, Ozturk S. Amiodarone induces a caffeine-inhibited, MID1-depedent rise in free cytoplasmic calcium in Saccharomyces cerevisiae. Mol Microbiol. 2003;47:223-34 pubmed
    ..b>Mid1p and Cch1p are believed to function in a capacitive calcium entry (CCE)-like process...