MEK1

Summary

Gene Symbol: MEK1
Description: serine/threonine protein kinase MEK1
Alias: MRE4, serine/threonine protein kinase MEK1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Rockmill B, Roeder G. A meiosis-specific protein kinase homolog required for chromosome synapsis and recombination. Genes Dev. 1991;5:2392-404 pubmed
    The mek1 (meiotic kinase) mutant of Saccharomyces cerevisiae was isolated in a screen for sporulation-proficient, meiotic-lethal mutants. Diploids homozygous for a mek1 null mutation produce only 13% viable spores...
  2. Xu L, Weiner B, Kleckner N. Meiotic cells monitor the status of the interhomolog recombination complex. Genes Dev. 1997;11:106-18 pubmed
    ..We report here the effects of mutations in two other (meiosis-specific) genes, RED1 and MEK1/MRE4, that encode a chromosome structure component and a protein kinase, respectively...
  3. Hollingsworth N, Ponte L. Genetic interactions between HOP1, RED1 and MEK1 suggest that MEK1 regulates assembly of axial element components during meiosis in the yeast Saccharomyces cerevisiae. Genetics. 1997;147:33-42 pubmed
    ..Genetic and cytological data suggest that three meiosis-specific genes, HOP1, RED1 and MEK1, are involved in axial element formation in the yeast Saccharomyces cerevisiae...
  4. Leem S, Ogawa H. The MRE4 gene encodes a novel protein kinase homologue required for meiotic recombination in Saccharomyces cerevisiae. Nucleic Acids Res. 1992;20:449-57 pubmed
    The MRE4 gene was cloned by complementation of the defects of meiotic recombination and haploidization in an mre4-1 mutant. Disruption of MRE4 resulted in reduced meiotic recombination and spore inviability...
  5. Niu H, Wan L, Baumgartner B, Schaefer D, Loidl J, Hollingsworth N. Partner choice during meiosis is regulated by Hop1-promoted dimerization of Mek1. Mol Biol Cell. 2005;16:5804-18 pubmed
    ..choice is due in part to a barrier to sister chromatid repair (BSCR) created by the meiosis-specific kinase, Mek1, in a complex with two other meiosis-specific proteins, Hop1 and Red1...
  6. Zanders S, Sonntag Brown M, Chen C, Alani E. Pch2 modulates chromatid partner choice during meiotic double-strand break repair in Saccharomyces cerevisiae. Genetics. 2011;188:511-21 pubmed publisher
    ..Results from genetic epistasis analyses involving spo13?, rad54?, and mek1/MEK1 alleles and an intersister recombination reporter assay are also consistent with Pch2 acting to limit ..
  7. Wu H, Ho H, Burgess S. Mek1 kinase governs outcomes of meiotic recombination and the checkpoint response. Curr Biol. 2010;20:1707-16 pubmed publisher
    ..In yeast, activation of Mek1 kinase upholds this bias...
  8. Niu H, Wan L, Busygina V, Kwon Y, Allen J, Li X, et al. Regulation of meiotic recombination via Mek1-mediated Rad54 phosphorylation. Mol Cell. 2009;36:393-404 pubmed publisher
    ..the bias for interhomolog recombination in meiosis requires the Dmc1 recombinase and the meiosis-specific kinase Mek1, which suppresses engagement of sister chromatids by the mitotic recombinase Rad51...
  9. Bailis J, Roeder G. Pachytene exit controlled by reversal of Mek1-dependent phosphorylation. Cell. 2000;101:211-21 pubmed
    ..This pachytene checkpoint requires two meiotic chromosomal proteins, Red1 and Mek1; Mek1 is a kinase that phosphorylates Red1...

More Information

Publications28

  1. Wan L, de Los Santos T, Zhang C, Shokat K, Hollingsworth N. Mek1 kinase activity functions downstream of RED1 in the regulation of meiotic double strand break repair in budding yeast. Mol Biol Cell. 2004;15:11-23 pubmed
    Three meiosis-specific chromosomal components in budding yeast, Mek1, Red1, and Hop1, are required for recombination, proper segregation of homologs, and the meiotic recombination checkpoint. Mek1 is a protein kinase...
  2. Lo H, Hollingsworth N. Using the semi-synthetic epitope system to identify direct substrates of the meiosis-specific budding yeast kinase, Mek1. Methods Mol Biol. 2011;745:135-49 pubmed publisher
    Recent studies have shown that the meiosis-specific kinase, Mek1, plays a key role in promoting recombination between homologous chromosomes during meiosis in budding yeast by suppressing recombination between sister chromatids, as well ..
  3. Chen X, Suhandynata R, Sandhu R, Rockmill B, Mohibullah N, Niu H, et al. Phosphorylation of the Synaptonemal Complex Protein Zip1 Regulates the Crossover/Noncrossover Decision during Yeast Meiosis. PLoS Biol. 2015;13:e1002329 pubmed publisher
    ..Zip1 4S phosphorylation is promoted both by double-strand breaks (DSBs) and the meiosis-specific kinase, MEK1/MRE4, demonstrating a role for MEK1 in the regulation of interhomolog crossover formation, as well as interhomolog bias...
  4. Peoples T, Dean E, Gonzalez O, Lambourne L, Burgess S. Close, stable homolog juxtaposition during meiosis in budding yeast is dependent on meiotic recombination, occurs independently of synapsis, and is distinct from DSB-independent pairing contacts. Genes Dev. 2002;16:1682-95 pubmed
    ..These data suggest that stable, close homolog juxtaposition in yeast is distinct from pre-DSB pairing interactions, requires both DSB and SEI formation, but does not depend on crossovers or SC. ..
  5. de los Santos T, Hollingsworth N. Red1p, a MEK1-dependent phosphoprotein that physically interacts with Hop1p during meiosis in yeast. J Biol Chem. 1999;274:1783-90 pubmed
    ..cerevisiae, HOP1 and RED1, is critical for proper assembly and function of the SC. A third meiosis-specific gene, MEK1, which encodes a putative serine/threonine protein kinase, is also important for proper AE function, suggesting ..
  6. Ajimura M, Leem S, Ogawa H. Identification of new genes required for meiotic recombination in Saccharomyces cerevisiae. Genetics. 1993;133:51-66 pubmed
    ..groups (MRE2 and MRE11) and eight previously identified groups, which include SPO11, HOP1, REC114, MRE4/MEK1 and genes in the RAD52 epistasis group...
  7. Burns N, Grimwade B, Ross MacDonald P, Choi E, Finberg K, Roeder G, et al. Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae. Genes Dev. 1994;8:1087-105 pubmed
    ..staining pattern, Ty1-beta-gal fusion proteins localize to cytoplasmic dots, and the meiosis-specific Mek1/Mre4-beta-gal and Spo11-beta-gal fusion proteins reside in the nucleus...
  8. Callender T, Laureau R, Wan L, Chen X, Sandhu R, Laljee S, et al. Mek1 Down Regulates Rad51 Activity during Yeast Meiosis by Phosphorylation of Hed1. PLoS Genet. 2016;12:e1006226 pubmed publisher
    ..This inhibition is dependent upon the meiosis-specific kinase Mek1 and occurs through two different mechanisms that prevent complex formation with the Rad51 accessory factor Rad54: (..
  9. Najor N, Weatherford L, Brush G. Prevention of DNA Rereplication Through a Meiotic Recombination Checkpoint Response. G3 (Bethesda). 2016;6:3869-3881 pubmed publisher
    ..Downstream of MEC1, MEK1 is required through its function to inhibit repair between sister chromatids...
  10. Ontoso D, Acosta I, van Leeuwen F, Freire R, San Segundo P. Dot1-dependent histone H3K79 methylation promotes activation of the Mek1 meiotic checkpoint effector kinase by regulating the Hop1 adaptor. PLoS Genet. 2013;9:e1003262 pubmed publisher
    ..We also define the phosphorylation events involving activation of the meiotic checkpoint effector Mek1 kinase. Dot1 is required for Mek1 autophosphorylation, but not for its Mec1/Tel1-dependent phosphorylation...
  11. Cooper K, Mallory M, Guacci V, Lowe K, Strich R. Pds1p is required for meiotic recombination and prophase I progression in Saccharomyces cerevisiae. Genetics. 2009;181:65-79 pubmed publisher
    ..This role is meiosis specific as Mcd1p destruction is not altered in vegetative pds1Delta cultures. These results define a previously undescribed role for Pds1p in cohesin maintenance, recombination, and meiotic progression. ..
  12. Callender T, Hollingsworth N. Mek1 suppression of meiotic double-strand break repair is specific to sister chromatids, chromosome autonomous and independent of Rec8 cohesin complexes. Genetics. 2010;185:771-82 pubmed publisher
    ..In budding yeast, interhomolog bias is created in part by the activity of a meiosis-specific kinase, Mek1, which is localized to the protein cores of condensed sister chromatids...
  13. Lo H, Wan L, Rosebrock A, Futcher B, Hollingsworth N. Cdc7-Dbf4 regulates NDT80 transcription as well as reductional segregation during budding yeast meiosis. Mol Biol Cell. 2008;19:4956-67 pubmed publisher
    ..The use of the same kinase to regulate several distinct meiosis-specific processes may be important for the coordination of these processes during meiosis. ..
  14. Lao J, Cloud V, Huang C, Grubb J, Thacker D, Lee C, et al. Meiotic crossover control by concerted action of Rad51-Dmc1 in homolog template bias and robust homeostatic regulation. PLoS Genet. 2013;9:e1003978 pubmed publisher
    ..This function of Dmc1 is independent of Hed1, but requires the meiotic kinase, Mek1. Hed1 makes a relatively minor contribution to homolog bias, but nonetheless this is important for normal ..
  15. Prugar E, Burnett C, Chen X, Hollingsworth N. Coordination of Double Strand Break Repair and Meiotic Progression in Yeast by a Mek1-Ndt80 Negative Feedback Loop. Genetics. 2017;206:497-512 pubmed publisher
    ..The meiosis-specific kinase Mek1 is critical for this regulation...
  16. Subramanian V, MacQueen A, Vader G, Shinohara M, Sanchez A, Borde V, et al. Chromosome Synapsis Alleviates Mek1-Dependent Suppression of Meiotic DNA Repair. PLoS Biol. 2016;14:e1002369 pubmed publisher
    ..than the equally available sister chromatid, a bias that in Saccharomyces cerevisiae depends on the meiotic kinase, Mek1. Mek1 is thought to mediate repair template bias by specifically suppressing sister-directed repair...
  17. Bailis J, Smith A, Roeder G. Bypass of a meiotic checkpoint by overproduction of meiotic chromosomal proteins. Mol Cell Biol. 2000;20:4838-48 pubmed
    ..Overproduction of either the meiotic chromosomal protein Red1 or the meiotic kinase Mek1 bypasses this checkpoint, allowing zip1 cells to sporulate...
  18. Bailis J, Roeder G. Synaptonemal complex morphogenesis and sister-chromatid cohesion require Mek1-dependent phosphorylation of a meiotic chromosomal protein. Genes Dev. 1998;12:3551-63 pubmed
    Development of yeast meiotic chromosome cores into full-length synaptonemal complexes requires the MEK1 gene product, a meiosis-specific protein kinase homolog...
  19. Kniewel R, Murakami H, Liu Y, Ito M, Ohta K, Hollingsworth N, et al. Histone H3 Threonine 11 Phosphorylation Is Catalyzed Directly by the Meiosis-Specific Kinase Mek1 and Provides a Molecular Readout of Mek1 Activity in Vivo. Genetics. 2017;207:1313-1333 pubmed publisher
    i>Saccharomyces cerevisiae Mek1 is a CHK2/Rad53-family kinase that regulates meiotic recombination and progression upon its activation in response to DNA double-strand breaks (DSBs)...