MEH1

Summary

Gene Symbol: MEH1
Description: Meh1p
Alias: EGO1, GSE2, Meh1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Powis K, Zhang T, Panchaud N, Wang R, De Virgilio C, Ding J. Crystal structure of the Ego1-Ego2-Ego3 complex and its role in promoting Rag GTPase-dependent TORC1 signaling. Cell Res. 2015;25:1043-59 pubmed publisher
    ..Amino acid-dependent activation of TORC1 is mediated via the yeast EGO complex (EGOC) consisting of Gtr1, Gtr2, Ego1, and Ego3...
  2. Gao X, Wang J, Keppler Ross S, Dean N. ERS1 encodes a functional homologue of the human lysosomal cystine transporter. FEBS J. 2005;272:2497-511 pubmed
    ..A genetic screen for multicopy suppressors of an ers1Delta yeast strain identified a novel gene, MEH1, which is implicated in regulating Ers1 function...
  3. Nadolski M, Linder M. Molecular recognition of the palmitoylation substrate Vac8 by its palmitoyltransferase Pfa3. J Biol Chem. 2009;284:17720-30 pubmed publisher
    ..This was confirmed by use of a chimeric protein in which the SH4 domain of Vac8 was swapped for that of Meh1, another palmitoylated and N-myristoylated protein in yeast...
  4. Sekiguchi T, Kamada Y, Furuno N, Funakoshi M, Kobayashi H. Amino acid residues required for Gtr1p-Gtr2p complex formation and its interactions with the Ego1p-Ego3p complex and TORC1 components in yeast. Genes Cells. 2014;19:449-63 pubmed publisher
    ..The GTP-bound Gtr2p suppressed a Kog1p mutation. Our findings indicate that the interactions of the Gtr1p-Gtr2p complex with the Ego1p-Ego3p complex and TORC1 components Kog1p and Tco89p play a role in TORC1 function. ..
  5. Kira S, Kumano Y, Ukai H, Takeda E, Matsuura A, Noda T. Dynamic relocation of the TORC1-Gtr1/2-Ego1/2/3 complex is regulated by Gtr1 and Gtr2. Mol Biol Cell. 2016;27:382-96 pubmed publisher
    ..Here we show that Ego consists of Ego1 and Ego3, and novel subunit Ego2...
  6. Dubouloz F, Deloche O, Wanke V, Cameroni E, De Virgilio C. The TOR and EGO protein complexes orchestrate microautophagy in yeast. Mol Cell. 2005;19:15-26 pubmed
    ..subfamily of Ras-related GTPases, Gtr2, which acts in a vacuolar membrane-associated protein complex together with Ego1 and Ego3 to ensure proper exit from rapamycin-induced growth arrest...
  7. Hou H, Subramanian K, LaGrassa T, Markgraf D, Dietrich L, Urban J, et al. The DHHC protein Pfa3 affects vacuole-associated palmitoylation of the fusion factor Vac8. Proc Natl Acad Sci U S A. 2005;102:17366-71 pubmed
    ..yeast vacuoles: the fusion factor Vac8, the kinase Yck3, and a novel adaptor protein implicated in microautophagy, Meh1. Here, we analyze the role that putative acyltransferases of the DHHC family play in their localization and ..
  8. Gao M, Kaiser C. A conserved GTPase-containing complex is required for intracellular sorting of the general amino-acid permease in yeast. Nat Cell Biol. 2006;8:657-67 pubmed
    ..Together, these studies provide evidence that the GSE complex has a key role in trafficking Gap1p out of the endosome and may serve as coat proteins in this process. ..
  9. Wang Y, Kurihara Y, Sato T, Toh H, Kobayashi H, Sekiguchi T. Gtr1p differentially associates with Gtr2p and Ego1p. Gene. 2009;437:32-8 pubmed
    ..Gtr2p mutants S23N and T44N, but not Q66L, rescued the gtr2 disruptant. Our findings indicate that the formation of heterodimers by Gtr1p differs between Gtr2p and Ego1p. ..

More Information

Publications13

  1. Binda M, Péli Gulli M, Bonfils G, Panchaud N, Urban J, Sturgill T, et al. The Vam6 GEF controls TORC1 by activating the EGO complex. Mol Cell. 2009;35:563-73 pubmed publisher
    ..Thus, in addition to its regulatory role in homotypic vacuolar fusion and vacuole protein sorting within the HOPS complex, Vam6 also controls TORC1 function by activating the Gtr1 subunit of the EGO complex. ..
  2. Kogan K, Spear E, Kaiser C, Fass D. Structural conservation of components in the amino acid sensing branch of the TOR pathway in yeast and mammals. J Mol Biol. 2010;402:388-98 pubmed publisher
    ..Together, these findings reveal that key components of the TOR signaling pathway are structurally conserved between yeast and mammals, despite divergence of sequence to a degree that thwarts detection through simple homology searches. ..
  3. Zhang T, Péli Gulli M, Yang H, De Virgilio C, Ding J. Ego3 functions as a homodimer to mediate the interaction between Gtr1-Gtr2 and Ego1 in the ego complex to activate TORC1. Structure. 2012;20:2151-60 pubmed publisher
    The yeast EGO complex, consisting of Gtr1, Gtr2, Ego1, and Ego3, localizes to the endosomal and vacuolar membranes and plays a pivotal role in cell growth and autophagy regulation through relaying amino acid signals to activate TORC1...
  4. Kim A, Cunningham K. A LAPF/phafin1-like protein regulates TORC1 and lysosomal membrane permeabilization in response to endoplasmic reticulum membrane stress. Mol Biol Cell. 2015;26:4631-45 pubmed publisher
    ..Rapamycin prevented the death of the pathogenic yeast Candida albicans during exposure to fluconazole plus a calcineurin inhibitor, suggesting that TORC1 broadly promotes sensitivity to fungistats in yeasts. ..